Click here to switch to the map view.

The map label for this gene is alsT [H]

Identifier: 229917563

GI number: 229917563

Start: 1809520

End: 1810992

Strand: Direct

Name: alsT [H]

Synonym: EAT1b_1838

Alternate gene names: 229917563

Gene position: 1809520-1810992 (Clockwise)

Preceding gene: 229917561

Following gene: 229917566

Centisome position: 60.32

GC content: 49.49

Gene sequence:

>1473_bases
ATGGAAGCGATTCAAGGTATGTTACAAGGAAGCGTCGACTTTTTGAACAACATTTTGTGGTCGTACGTATTGATCGCCGT
GCTCGTCGGAGCCGGGATCTACTTCACAGTCAGAACACGATTTATGCAGTTCCGTTACTTAAAAGAAATGTTCCGTACGA
TTACAGATAAATCGGACGTTACACTTTCAAAAGACGGGAAGAGTATTTCTTCAATGAAATCATTCTTTATCGGTGCTGCG
ACTCGAATCGGTACAGGTAACCTTGCCGGGGTCACGGTAGCGGTCACACTTGGTGGACCGGGAGCGGTCTTCTGGATGTG
GATCGTCGCCCTTCTAGGTGGTGCGACAGCCATGATTGAAAGCACGCTCGCTCAAGTATACAAAGTGAAAGACGATGTCG
CGTACCGCGGTGGTCCGGCTTACTACATCGAAAAAGGTTTAAACAACCGTGCACTTGGAATCGTATTCGCGGTTCTCATT
GCTGTCACATTCGGACTCATCTTCAACTCTGTTCAATCGAACACAATCGCTGCAGCATTCGATAACGCATTCGGAATCGA
TGCAGCAATCGGTGGAGCAATCTTGGTTGTTCTTACTGGTCTTGTCATCTTTGGTGGTGTACAACGTGTCGCGAACTTCT
CGGCAATCGTCGTACCTGTCATGGCTGTTCTCTATCTTGCCATCGCACTCTACGTTGTCGTTGTGAACTTCGCTGAACTT
CCAGGCGTCTTCGCTATGATTTTCCAAAGCGCATTTGGTTTGAACGAAGCAGTCGGTGGTTCAATCGGTGCTGCAATCTC
AATGGGTGTACGCCGTGGACTCTTCTCGAACGAAGCAGGTATGGGTTCTGCACCAAACGCAGCAGCAGCTGCTGAAGTAT
CGCACCCTGCGAAACAAGGTTTCCTTCAAGCACTCGGTGTATTCTTGGATACGTTGATCGTATGTACAGCAACTGCAGCG
ATCATCCTTCTCTCAGACAGCTACGCTGCTGGAAACGGTGAAGGGATTGTCCTTCTTCAAAACGCATTGACTGAGCAAAT
CGGAGCACTTGCTCCAGCATTCATCGCTGTCAGCGTCTTCTTGTTCGCCTTCAGCTCGATCGCAGGAAGCTACTACTACG
GTGAAACGAACATCGAATTCATTAAGAAGAGCAAAGGTGCAGTCCTCGGATTCCGTCTCGCGACAATGGCATTCGTCTTC
ATCGGAGCGGTCGCAAGCCTCGGATTCGTCTGGAGCCTCGCTGACCTCTTCATGGCAGGGATGACATTGATCAACATCGC
GGCAATCACATTGCTCGGTGGCGTTGCCTTCAAAGTTCTCAAAGACTACGAAGAGCAACGAGCTCAAGGTCTCAACCCAC
GCTTCTCGGCTCGTAAACTCGGTATCGAGAATACAGAGTGCTGGGATGTGGAAGAAGACGAAGTGGCACAAGGTACTGTT
CAAGCAGCAGCAGCCGACGCTTCAAAATCATAA

Upstream 100 bases:

>100_bases
AAAAATTATTGCTAATTGTCAGAATCTTTGTATAATTCAGAACTAAGACATCATAAAAAATTCTCAACTTCAGATTCACA
CAACAGGGAGGAACTTATTC

Downstream 100 bases:

>100_bases
TTCGTGAAATGCAACAAACAGGGACCCTGCTAATCGGGGTCCCTGTCTTTTTATGCATGATGACGTATCGGCTTGACGGT
TTGTTCCGTCTCCGATTTGT

Product: amino acid carrier protein

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MEAIQGMLQGSVDFLNNILWSYVLIAVLVGAGIYFTVRTRFMQFRYLKEMFRTITDKSDVTLSKDGKSISSMKSFFIGAA
TRIGTGNLAGVTVAVTLGGPGAVFWMWIVALLGGATAMIESTLAQVYKVKDDVAYRGGPAYYIEKGLNNRALGIVFAVLI
AVTFGLIFNSVQSNTIAAAFDNAFGIDAAIGGAILVVLTGLVIFGGVQRVANFSAIVVPVMAVLYLAIALYVVVVNFAEL
PGVFAMIFQSAFGLNEAVGGSIGAAISMGVRRGLFSNEAGMGSAPNAAAAAEVSHPAKQGFLQALGVFLDTLIVCTATAA
IILLSDSYAAGNGEGIVLLQNALTEQIGALAPAFIAVSVFLFAFSSIAGSYYYGETNIEFIKKSKGAVLGFRLATMAFVF
IGAVASLGFVWSLADLFMAGMTLINIAAITLLGGVAFKVLKDYEEQRAQGLNPRFSARKLGIENTECWDVEEDEVAQGTV
QAAAADASKS

Sequences:

>Translated_490_residues
MEAIQGMLQGSVDFLNNILWSYVLIAVLVGAGIYFTVRTRFMQFRYLKEMFRTITDKSDVTLSKDGKSISSMKSFFIGAA
TRIGTGNLAGVTVAVTLGGPGAVFWMWIVALLGGATAMIESTLAQVYKVKDDVAYRGGPAYYIEKGLNNRALGIVFAVLI
AVTFGLIFNSVQSNTIAAAFDNAFGIDAAIGGAILVVLTGLVIFGGVQRVANFSAIVVPVMAVLYLAIALYVVVVNFAEL
PGVFAMIFQSAFGLNEAVGGSIGAAISMGVRRGLFSNEAGMGSAPNAAAAAEVSHPAKQGFLQALGVFLDTLIVCTATAA
IILLSDSYAAGNGEGIVLLQNALTEQIGALAPAFIAVSVFLFAFSSIAGSYYYGETNIEFIKKSKGAVLGFRLATMAFVF
IGAVASLGFVWSLADLFMAGMTLINIAAITLLGGVAFKVLKDYEEQRAQGLNPRFSARKLGIENTECWDVEEDEVAQGTV
QAAAADASKS
>Mature_490_residues
MEAIQGMLQGSVDFLNNILWSYVLIAVLVGAGIYFTVRTRFMQFRYLKEMFRTITDKSDVTLSKDGKSISSMKSFFIGAA
TRIGTGNLAGVTVAVTLGGPGAVFWMWIVALLGGATAMIESTLAQVYKVKDDVAYRGGPAYYIEKGLNNRALGIVFAVLI
AVTFGLIFNSVQSNTIAAAFDNAFGIDAAIGGAILVVLTGLVIFGGVQRVANFSAIVVPVMAVLYLAIALYVVVVNFAEL
PGVFAMIFQSAFGLNEAVGGSIGAAISMGVRRGLFSNEAGMGSAPNAAAAAEVSHPAKQGFLQALGVFLDTLIVCTATAA
IILLSDSYAAGNGEGIVLLQNALTEQIGALAPAFIAVSVFLFAFSSIAGSYYYGETNIEFIKKSKGAVLGFRLATMAFVF
IGAVASLGFVWSLADLFMAGMTLINIAAITLLGGVAFKVLKDYEEQRAQGLNPRFSARKLGIENTECWDVEEDEVAQGTV
QAAAADASKS

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=480, Percent_Identity=41.25, Blast_Score=337, Evalue=1e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 51443; Mature: 51443

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00873 NA_ALANINE_SYMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAIQGMLQGSVDFLNNILWSYVLIAVLVGAGIYFTVRTRFMQFRYLKEMFRTITDKSDV
CHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCE
TLSKDGKSISSMKSFFIGAATRIGTGNLAGVTVAVTLGGPGAVFWMWIVALLGGATAMIE
EECCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCHHHHHH
STLAQVYKVKDDVAYRGGPAYYIEKGLNNRALGIVFAVLIAVTFGLIFNSVQSNTIAAAF
HHHHHHHHHHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
DNAFGIDAAIGGAILVVLTGLVIFGGVQRVANFSAIVVPVMAVLYLAIALYVVVVNFAEL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PGVFAMIFQSAFGLNEAVGGSIGAAISMGVRRGLFSNEAGMGSAPNAAAAAEVSHPAKQG
HHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCHHHHH
FLQALGVFLDTLIVCTATAAIILLSDSYAAGNGEGIVLLQNALTEQIGALAPAFIAVSVF
HHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHH
LFAFSSIAGSYYYGETNIEFIKKSKGAVLGFRLATMAFVFIGAVASLGFVWSLADLFMAG
HHHHHHHHCCEEECCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MTLINIAAITLLGGVAFKVLKDYEEQRAQGLNPRFSARKLGIENTECWDVEEDEVAQGTV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHH
QAAAADASKS
HHHHHCCCCC
>Mature Secondary Structure
MEAIQGMLQGSVDFLNNILWSYVLIAVLVGAGIYFTVRTRFMQFRYLKEMFRTITDKSDV
CHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCE
TLSKDGKSISSMKSFFIGAATRIGTGNLAGVTVAVTLGGPGAVFWMWIVALLGGATAMIE
EECCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCHHHHHH
STLAQVYKVKDDVAYRGGPAYYIEKGLNNRALGIVFAVLIAVTFGLIFNSVQSNTIAAAF
HHHHHHHHHHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
DNAFGIDAAIGGAILVVLTGLVIFGGVQRVANFSAIVVPVMAVLYLAIALYVVVVNFAEL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PGVFAMIFQSAFGLNEAVGGSIGAAISMGVRRGLFSNEAGMGSAPNAAAAAEVSHPAKQG
HHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCHHHHH
FLQALGVFLDTLIVCTATAAIILLSDSYAAGNGEGIVLLQNALTEQIGALAPAFIAVSVF
HHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHH
LFAFSSIAGSYYYGETNIEFIKKSKGAVLGFRLATMAFVFIGAVASLGFVWSLADLFMAG
HHHHHHHHCCEEECCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MTLINIAAITLLGGVAFKVLKDYEEQRAQGLNPRFSARKLGIENTECWDVEEDEVAQGTV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHH
QAAAADASKS
HHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969507; 9384377; 7710280 [H]