Click here to switch to the map view.

The map label for this gene is rluF [H]

Identifier: 229917541

GI number: 229917541

Start: 1781712

End: 1782422

Strand: Reverse

Name: rluF [H]

Synonym: EAT1b_1816

Alternate gene names: 229917541

Gene position: 1782422-1781712 (Counterclockwise)

Preceding gene: 229917543

Following gene: 229917538

Centisome position: 59.42

GC content: 51.2

Gene sequence:

>711_bases
ATGCGCATCAATAAATTCATCAGTGAATCAGGCAAGGCGTCCCGTCGCCAAGCCGACCGTCTCGTCGAAGAGGGACGCGT
CACCATCAATGGGAAAAAAGCAAAAATCGGCGACCAAGTCAAACCGGGCGATGAAGTCCTCGTCAACGGTTCTGTCGCAC
GGGTTGCCCGAAACAACGTCTACATCGCCGTCCATAAGCCGGTCGGGATCACGAGTACGACAGAAAAAAAAGTAAAAGGG
AACTTGGTAGACATGGTCAACCACCCGCTCCGCATCTTCAACATCGGTCGTCTTGATAAAGACTCGGAAGGTCTCATCCT
CATGACGAATGACGGCGATATCGTCAACGAGATTCTCCGTGCAGAAGGCGAGCACGAGAAAGAATACATCGTCTCGGTTG
ATAAGCCAATCACACCAGAGTTCGTCGAGCAGATGGAAGCCGGTGTCAAAATCCTTGGCCAGAAGACACTTCCGTGTAAA
GTCCGTCCGCTTTCAAAGTTCGATTTCAACATTACCCTCACGCAAGGACTCAACCGCCAAATCCGTCGTATGTGTGAGGC
GCTCGGATACGAAGTATTCCGCCTCCAACGTGTTCGTGTCATGAACATCACGCTCGGGAAACTGCCGCCTGGACAATGGC
GCGATCTTTCGAAAAAAGAGCGTGAGCAGCTGTTCCGCGAATTGAATTACGAACCGAAAGAATGGTACTAA

Upstream 100 bases:

>100_bases
GCCTCCATGTCATCCCTCCTCTCCCGTTCGTTTCCCGCTTTCGAGAGTTTATAAAATGCGTTATAGTGAGAGGACGAAGC
CAAACAAAGGACGTGTACAC

Downstream 100 bases:

>100_bases
AAAGCAGCCTGAGTCGTCTCAGGCTGCTTTATTCTGGTCGAAGTGATGAGACATGGTCGGTCTCACGACGAATGAAACGT
TTGAGTTGAGCGCTCAAGTC

Product: RNA-binding S4 domain protein

Products: NA

Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F [H]

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MRINKFISESGKASRRQADRLVEEGRVTINGKKAKIGDQVKPGDEVLVNGSVARVARNNVYIAVHKPVGITSTTEKKVKG
NLVDMVNHPLRIFNIGRLDKDSEGLILMTNDGDIVNEILRAEGEHEKEYIVSVDKPITPEFVEQMEAGVKILGQKTLPCK
VRPLSKFDFNITLTQGLNRQIRRMCEALGYEVFRLQRVRVMNITLGKLPPGQWRDLSKKEREQLFRELNYEPKEWY

Sequences:

>Translated_236_residues
MRINKFISESGKASRRQADRLVEEGRVTINGKKAKIGDQVKPGDEVLVNGSVARVARNNVYIAVHKPVGITSTTEKKVKG
NLVDMVNHPLRIFNIGRLDKDSEGLILMTNDGDIVNEILRAEGEHEKEYIVSVDKPITPEFVEQMEAGVKILGQKTLPCK
VRPLSKFDFNITLTQGLNRQIRRMCEALGYEVFRLQRVRVMNITLGKLPPGQWRDLSKKEREQLFRELNYEPKEWY
>Mature_236_residues
MRINKFISESGKASRRQADRLVEEGRVTINGKKAKIGDQVKPGDEVLVNGSVARVARNNVYIAVHKPVGITSTTEKKVKG
NLVDMVNHPLRIFNIGRLDKDSEGLILMTNDGDIVNEILRAEGEHEKEYIVSVDKPITPEFVEQMEAGVKILGQKTLPCK
VRPLSKFDFNITLTQGLNRQIRRMCEALGYEVFRLQRVRVMNITLGKLPPGQWRDLSKKEREQLFRELNYEPKEWY

Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=228, Percent_Identity=60.5263157894737, Blast_Score=265, Evalue=2e-72,
Organism=Escherichia coli, GI1787524, Length=251, Percent_Identity=32.2709163346614, Blast_Score=109, Evalue=1e-25,
Organism=Escherichia coli, GI1788510, Length=226, Percent_Identity=29.646017699115, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI87081838, Length=145, Percent_Identity=28.9655172413793, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 26986; Mature: 26986

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRINKFISESGKASRRQADRLVEEGRVTINGKKAKIGDQVKPGDEVLVNGSVARVARNNV
CCCHHHHHCCCCHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCEEEECCCCCEEECCCE
YIAVHKPVGITSTTEKKVKGNLVDMVNHPLRIFNIGRLDKDSEGLILMTNDGDIVNEILR
EEEEECCCCCCCCCHHHHCCCHHHHHCCCEEEEEECCCCCCCCCEEEEECCCHHHHHHHH
AEGEHEKEYIVSVDKPITPEFVEQMEAGVKILGQKTLPCKVRPLSKFDFNITLTQGLNRQ
CCCCCCCEEEEECCCCCCHHHHHHHHHHHHEECCCCCCEEECCCCCCCEEEEEHHHHHHH
IRRMCEALGYEVFRLQRVRVMNITLGKLPPGQWRDLSKKEREQLFRELNYEPKEWY
HHHHHHHHCHHHHHHHHEEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRINKFISESGKASRRQADRLVEEGRVTINGKKAKIGDQVKPGDEVLVNGSVARVARNNV
CCCHHHHHCCCCHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCEEEECCCCCEEECCCE
YIAVHKPVGITSTTEKKVKGNLVDMVNHPLRIFNIGRLDKDSEGLILMTNDGDIVNEILR
EEEEECCCCCCCCCHHHHCCCHHHHHCCCEEEEEECCCCCCCCCEEEEECCCHHHHHHHH
AEGEHEKEYIVSVDKPITPEFVEQMEAGVKILGQKTLPCKVRPLSKFDFNITLTQGLNRQ
CCCCCCCEEEEECCCCCCHHHHHHHHHHHHEECCCCCCEEECCCCCCCEEEEEHHHHHHH
IRRMCEALGYEVFRLQRVRVMNITLGKLPPGQWRDLSKKEREQLFRELNYEPKEWY
HHHHHHHHCHHHHHHHHEEEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]