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The map label for this gene is lcfB [H]

Identifier: 229917488

GI number: 229917488

Start: 1729868

End: 1731274

Strand: Direct

Name: lcfB [H]

Synonym: EAT1b_1763

Alternate gene names: 229917488

Gene position: 1729868-1731274 (Clockwise)

Preceding gene: 229917487

Following gene: 229917489

Centisome position: 57.66

GC content: 50.46

Gene sequence:

>1407_bases
ATGACGCTTTTTCAGACACTCGAGCAGTACATGGATCGTGAGGCGGTCTATGCGGACCGCTCTTATTCATATAGAGAGAT
GTTGGAAGTCGGGGACTCGATTTGTCAGGAAGTCGGCGAACGGTCGCTCGTCTTCTGCTTATGTGCCAACCGAGTCGAGT
CGCTCTTCGGATATATCGGCTTTCTCCGTGGACGGGTCGTCCCGGTCTTGCTCGATGCAGATATTCCAGTAGACCAATTG
CAGAAGTTGATGGAACGGTATCAACCGACTCACGTCTGGGCTCCTGAGACGAGGGAGGATTTGGCGAACTCGTTGGATGC
CTTCTATGGCTATGGTGACTACGCCTTATTTGAAACCGAAACATCGGTTCGCCACCAACTTCATGAGGAGCTGGCTCTTC
TGCTGACGACATCAGGTAGTACTGGAAGTCCGAAACTTGTACGCCTCACATACAAAAATCTGATGGCGAACGCTTCATCC
ATCATCGACTATCTCGACATCGATCAGACGGATAAGCCAATCACAACCCTTCCGATGAACTATTCGTATGGACTCTCCAT
CATCAACAGTCATCTGTTGAAAGGGGCGACCCTCATCCTGACAGACCGTTCGATCGTTCAAAAGGAGTTTTGGGAGCTGT
GTCGTGCCGAGAAGGCGACGACGTTTGGTGGGGTCCCGTTTCATTACGAGATGTTGAAACGATTAAAGTTTGAGACGTAC
GACCTCCCAAGTCTGAAGAAGTTGACACAAGCGGGTGGCAAGTTAGACCCGAAGCTTGCGCAGCACTTCGCAACAGTATG
TGAAGAGAAAGGGATTCAATTTTTCATCATGTACGGTCAGACGGAGGCGACGGCCCGGATGTCGTATTTACCGGATCGGA
TGCATGCGAAAAAAGCAGGGAGTATCGGAATCCCCATCCCTGGTGGAACGCTCTCGTTACGAGATGAAGCCGGGAACGAC
ATCAAAGAGGCGAACGTGACGGGCGAACTCGTGTATCAAGGTGATAACGTGTCGTTCGGATACGCAACATCGTTAGCGGA
CCTTTCAAAAGGTGACGACAATCAAGGTATCTTGCATACGGGTGACATGGCGTATCGCGACGAGGATGGATATTTCTTCA
TCGTCGGCCGAATCAAGCGCATGATCAAACTGTATGGCAGTCGAATCAGCCTAGACGAAGTCGAGGCGCTCCTTCGAGAG
CATGGACATGATTGCGTCTGTGCCGGGGAAGACGACCAGCTATACGTGTATACGTTACAAGATGACGTCGCACGCATTCA
AAAAATCATCAAAGAACAAATCAAGGTGAAAGGTGTTCGGGTGATGCAAATTGACGAGATTCCGCGAAATGCGTTCGGGA
AGATTCGTTATGCTGCATTGCCGAAGCGAGAACTTGTGACCACCTGA

Upstream 100 bases:

>100_bases
ATGCGTTTGACGTCTATATCGACTCGGATGAAATGACAGAGTTCCATTCGTATCAGTCTGGCATCGCGTTGCTGCAGCGC
CTCGGTGTGGACATCCAGTC

Downstream 100 bases:

>100_bases
TTGTCAGGAATCGGTACATCGTCAAGTTTCATAACATCAGGTGGTAAATATGACTTTTATTTCGATTGAATTTCTCTTAT
TTTTAACACTCATCGTTGGA

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MTLFQTLEQYMDREAVYADRSYSYREMLEVGDSICQEVGERSLVFCLCANRVESLFGYIGFLRGRVVPVLLDADIPVDQL
QKLMERYQPTHVWAPETREDLANSLDAFYGYGDYALFETETSVRHQLHEELALLLTTSGSTGSPKLVRLTYKNLMANASS
IIDYLDIDQTDKPITTLPMNYSYGLSIINSHLLKGATLILTDRSIVQKEFWELCRAEKATTFGGVPFHYEMLKRLKFETY
DLPSLKKLTQAGGKLDPKLAQHFATVCEEKGIQFFIMYGQTEATARMSYLPDRMHAKKAGSIGIPIPGGTLSLRDEAGND
IKEANVTGELVYQGDNVSFGYATSLADLSKGDDNQGILHTGDMAYRDEDGYFFIVGRIKRMIKLYGSRISLDEVEALLRE
HGHDCVCAGEDDQLYVYTLQDDVARIQKIIKEQIKVKGVRVMQIDEIPRNAFGKIRYAALPKRELVTT

Sequences:

>Translated_468_residues
MTLFQTLEQYMDREAVYADRSYSYREMLEVGDSICQEVGERSLVFCLCANRVESLFGYIGFLRGRVVPVLLDADIPVDQL
QKLMERYQPTHVWAPETREDLANSLDAFYGYGDYALFETETSVRHQLHEELALLLTTSGSTGSPKLVRLTYKNLMANASS
IIDYLDIDQTDKPITTLPMNYSYGLSIINSHLLKGATLILTDRSIVQKEFWELCRAEKATTFGGVPFHYEMLKRLKFETY
DLPSLKKLTQAGGKLDPKLAQHFATVCEEKGIQFFIMYGQTEATARMSYLPDRMHAKKAGSIGIPIPGGTLSLRDEAGND
IKEANVTGELVYQGDNVSFGYATSLADLSKGDDNQGILHTGDMAYRDEDGYFFIVGRIKRMIKLYGSRISLDEVEALLRE
HGHDCVCAGEDDQLYVYTLQDDVARIQKIIKEQIKVKGVRVMQIDEIPRNAFGKIRYAALPKRELVTT
>Mature_467_residues
TLFQTLEQYMDREAVYADRSYSYREMLEVGDSICQEVGERSLVFCLCANRVESLFGYIGFLRGRVVPVLLDADIPVDQLQ
KLMERYQPTHVWAPETREDLANSLDAFYGYGDYALFETETSVRHQLHEELALLLTTSGSTGSPKLVRLTYKNLMANASSI
IDYLDIDQTDKPITTLPMNYSYGLSIINSHLLKGATLILTDRSIVQKEFWELCRAEKATTFGGVPFHYEMLKRLKFETYD
LPSLKKLTQAGGKLDPKLAQHFATVCEEKGIQFFIMYGQTEATARMSYLPDRMHAKKAGSIGIPIPGGTLSLRDEAGNDI
KEANVTGELVYQGDNVSFGYATSLADLSKGDDNQGILHTGDMAYRDEDGYFFIVGRIKRMIKLYGSRISLDEVEALLREH
GHDCVCAGEDDQLYVYTLQDDVARIQKIIKEQIKVKGVRVMQIDEIPRNAFGKIRYAALPKRELVTT

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=334, Percent_Identity=27.2455089820359, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI42544132, Length=393, Percent_Identity=25.6997455470738, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI45580730, Length=361, Percent_Identity=24.9307479224377, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI145693145, Length=347, Percent_Identity=27.9538904899135, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1788107, Length=346, Percent_Identity=26.878612716763, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1789201, Length=320, Percent_Identity=29.0625, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1786810, Length=493, Percent_Identity=23.3265720081136, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17559526, Length=359, Percent_Identity=28.4122562674095, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71985884, Length=361, Percent_Identity=26.3157894736842, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17557194, Length=313, Percent_Identity=26.8370607028754, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17560308, Length=326, Percent_Identity=26.9938650306748, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17538037, Length=376, Percent_Identity=25.7978723404255, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17531443, Length=365, Percent_Identity=25.4794520547945, Blast_Score=72, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI71996755, Length=347, Percent_Identity=24.207492795389, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319699, Length=351, Percent_Identity=25.9259259259259, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6319264, Length=307, Percent_Identity=26.7100977198697, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323182, Length=305, Percent_Identity=26.2295081967213, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI21355181, Length=362, Percent_Identity=29.2817679558011, Blast_Score=99, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24648260, Length=296, Percent_Identity=26.3513513513513, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI18859661, Length=417, Percent_Identity=25.8992805755396, Blast_Score=89, Evalue=9e-18,
Organism=Drosophila melanogaster, GI281365686, Length=271, Percent_Identity=25.830258302583, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24581924, Length=283, Percent_Identity=26.1484098939929, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI21356947, Length=413, Percent_Identity=24.455205811138, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI20130357, Length=344, Percent_Identity=25.2906976744186, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21358303, Length=274, Percent_Identity=22.6277372262774, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24653035, Length=273, Percent_Identity=24.9084249084249, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI161076582, Length=273, Percent_Identity=24.9084249084249, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI21356441, Length=277, Percent_Identity=24.9097472924188, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24648257, Length=268, Percent_Identity=26.4925373134328, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 52877; Mature: 52746

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLFQTLEQYMDREAVYADRSYSYREMLEVGDSICQEVGERSLVFCLCANRVESLFGYIG
CCHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHH
FLRGRVVPVLLDADIPVDQLQKLMERYQPTHVWAPETREDLANSLDAFYGYGDYALFETE
HHHCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECC
TSVRHQLHEELALLLTTSGSTGSPKLVRLTYKNLMANASSIIDYLDIDQTDKPITTLPMN
HHHHHHHHHHHEEEEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCEEECCC
YSYGLSIINSHLLKGATLILTDRSIVQKEFWELCRAEKATTFGGVPFHYEMLKRLKFETY
CCHHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCC
DLPSLKKLTQAGGKLDPKLAQHFATVCEEKGIQFFIMYGQTEATARMSYLPDRMHAKKAG
CCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCHHHHHHCCC
SIGIPIPGGTLSLRDEAGNDIKEANVTGELVYQGDNVSFGYATSLADLSKGDDNQGILHT
CCCCCCCCCCEEECCCCCCCHHHCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCCEEEE
GDMAYRDEDGYFFIVGRIKRMIKLYGSRISLDEVEALLREHGHDCVCAGEDDQLYVYTLQ
CCCCEECCCCEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEECCCCEEEEEECC
DDVARIQKIIKEQIKVKGVRVMQIDEIPRNAFGKIRYAALPKRELVTT
HHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHCCEEEEECCCHHHCCC
>Mature Secondary Structure 
TLFQTLEQYMDREAVYADRSYSYREMLEVGDSICQEVGERSLVFCLCANRVESLFGYIG
CHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHH
FLRGRVVPVLLDADIPVDQLQKLMERYQPTHVWAPETREDLANSLDAFYGYGDYALFETE
HHHCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECC
TSVRHQLHEELALLLTTSGSTGSPKLVRLTYKNLMANASSIIDYLDIDQTDKPITTLPMN
HHHHHHHHHHHEEEEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCEEECCC
YSYGLSIINSHLLKGATLILTDRSIVQKEFWELCRAEKATTFGGVPFHYEMLKRLKFETY
CCHHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCC
DLPSLKKLTQAGGKLDPKLAQHFATVCEEKGIQFFIMYGQTEATARMSYLPDRMHAKKAG
CCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCHHHHHHCCC
SIGIPIPGGTLSLRDEAGNDIKEANVTGELVYQGDNVSFGYATSLADLSKGDDNQGILHT
CCCCCCCCCCEEECCCCCCCHHHCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCCEEEE
GDMAYRDEDGYFFIVGRIKRMIKLYGSRISLDEVEALLREHGHDCVCAGEDDQLYVYTLQ
CCCCEECCCCEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEECCCCEEEEEECC
DDVARIQKIIKEQIKVKGVRVMQIDEIPRNAFGKIRYAALPKRELVTT
HHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHCCEEEEECCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]