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The map label for this gene is rlmH

Identifier: 229917484

GI number: 229917484

Start: 1727293

End: 1727772

Strand: Direct

Name: rlmH

Synonym: EAT1b_1759

Alternate gene names: 229917484

Gene position: 1727293-1727772 (Clockwise)

Preceding gene: 229917483

Following gene: 229917485

Centisome position: 57.58

GC content: 47.08

Gene sequence:

>480_bases
ATGAATATTTCAATTATCACGGTCGGCAAACTGAAAGAAAAATATTTAAAGCAAGGAATTGCCGAGTATACAAAACGGCT
GTCTGCCTACGCAAAAGTGCAGGAAATCGAGGTCTCAGACGAAAAAGCACCCGAGCATTTGAGTGAGGCAGATATGTTAC
TAGTAAAACAAAAGGAAGGCGAGCGGATTTTGGCGAAGATATCCCCAGATACGCACGTCATCGCCTTGGCAATCAAAGGA
AAGCAGCGGACGAGTGAAGAGTTCGCCCGTGAACTCGATCAACTCGCGACGTACGGAAAGAGTAAGATCGCCTTCGTCAT
CGGAGGATCGCTCGGTCTGTCGGACGATGTGATGAAGCGAGCAGATGATACGATTTCGTTCTCGAAGATGACGTTCCCCC
ACCAACTCATGAAGCTCGTGTTGTGTGAGCAGATTTATCGGGCGTATCGAATCAATCGGAATGAGCCGTACCATAAATAA

Upstream 100 bases:

>100_bases
TGTCAAGCAAAAACTAATTATCAACATGTGGATTTGTGGATAACTCTGTGGATAACCCTATGGATAACTCCAAAAAAACT
GTGGAAAAAGGAAGAATTGG

Downstream 100 bases:

>100_bases
CTTCAAAACCTGTATCTCTTATATGAGATACAGGTTTTTTCTTGCTTAAAAATAGAAATACAATTGTTGTTGTAAGAATC
AGCCATTACAATATAGATAA

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MNISIITVGKLKEKYLKQGIAEYTKRLSAYAKVQEIEVSDEKAPEHLSEADMLLVKQKEGERILAKISPDTHVIALAIKG
KQRTSEEFARELDQLATYGKSKIAFVIGGSLGLSDDVMKRADDTISFSKMTFPHQLMKLVLCEQIYRAYRINRNEPYHK

Sequences:

>Translated_159_residues
MNISIITVGKLKEKYLKQGIAEYTKRLSAYAKVQEIEVSDEKAPEHLSEADMLLVKQKEGERILAKISPDTHVIALAIKG
KQRTSEEFARELDQLATYGKSKIAFVIGGSLGLSDDVMKRADDTISFSKMTFPHQLMKLVLCEQIYRAYRINRNEPYHK
>Mature_159_residues
MNISIITVGKLKEKYLKQGIAEYTKRLSAYAKVQEIEVSDEKAPEHLSEADMLLVKQKEGERILAKISPDTHVIALAIKG
KQRTSEEFARELDQLATYGKSKIAFVIGGSLGLSDDVMKRADDTISFSKMTFPHQLMKLVLCEQIYRAYRINRNEPYHK

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=159, Percent_Identity=30.188679245283, Blast_Score=88, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_EXISA (C4L022)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002886130.1
- ProteinModelPortal:   C4L022
- GeneID:   7870806
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_1759
- OMA:   TANHPYH
- ProtClustDB:   PRK00103
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR016051
- InterPro:   IPR003742
- PIRSF:   PIRSF004505
- TIGRFAMs:   TIGR00246

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 18090; Mature: 18090

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNISIITVGKLKEKYLKQGIAEYTKRLSAYAKVQEIEVSDEKAPEHLSEADMLLVKQKEG
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHEEEEECCCC
ERILAKISPDTHVIALAIKGKQRTSEEFARELDQLATYGKSKIAFVIGGSLGLSDDVMKR
CEEEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHH
ADDTISFSKMTFPHQLMKLVLCEQIYRAYRINRNEPYHK
CCCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNISIITVGKLKEKYLKQGIAEYTKRLSAYAKVQEIEVSDEKAPEHLSEADMLLVKQKEG
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHEEEEECCCC
ERILAKISPDTHVIALAIKGKQRTSEEFARELDQLATYGKSKIAFVIGGSLGLSDDVMKR
CEEEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHH
ADDTISFSKMTFPHQLMKLVLCEQIYRAYRINRNEPYHK
CCCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA