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The map label for this gene is engD [H]

Identifier: 229917469

GI number: 229917469

Start: 1712284

End: 1713384

Strand: Direct

Name: engD [H]

Synonym: EAT1b_1744

Alternate gene names: 229917469

Gene position: 1712284-1713384 (Clockwise)

Preceding gene: 229917468

Following gene: 229917470

Centisome position: 57.08

GC content: 49.23

Gene sequence:

>1101_bases
GTGGGATTAACAGCAGGAATTGTCGGATTGCCAAACGTCGGGAAATCGACATTGTTTAATGCGATTACACAAGCAGGAGT
CGAGGCGGCCAACTATCCGTTCGCGACAATCGACCCGAACGTCGGCGTCGTAGAAGTACCAGACGCGCGTCTTGATCGAT
TGACGGAACTCGTCAAACCGAAAAAGACAGTACCGACAGCATTTGAGTTTACAGATATCGCTGGAATCGTGAAAGGTGCA
TCAAAAGGGGAAGGTCTCGGAAACAAGTTCTTGGCGAACATTCGCGAAGTCGATGCAATTTGCCAAGTCGTCCGTTGTTT
CGAAGACGAGAACATCACGCACGTCGCAGGCCGTGTCTCGCCGATTGACGACATCACGACAATCAACCTTGAATTGATCT
TAGCTGACCTTGAGCAAGTCGATAAACGCATCGCACGTGTTCAAAAAATGGTGAAGGCAAAAGATAAAGGTGCGGTACAA
GAACTCGAAGCACTTGAAATCGTACGAGATGTGCTCGAGTCAGAGCGTCCGGCACGTGTTGCTGAATTATCAGAAGACCA
ACTCGCAATCGTCAAACACTTCCAATTGCTTACGATGAAGCCGATGCTTTACGTGGCGAACGTGAGTGAGGATGAAGTGG
CAGATGCCGATTCGAACGAGTTTGTACAAGCGGTCCGTGATCACGCGGCAACAGAAGGATCGAAAGTCATCGTCATCTGT
GCGCGAATCGAAGAAGAAATTTCAGAACTCGAACCGGAAGAACGCCTCGAATTCTTACAAGATCTCGGGATCGAAGAGTC
AGGACTTGATCAATTGATCCGTGCGGCCTACTCGACACTTGGCCTTGCGACATACTTCACGGCAGGTGAGAAGGAAGTTC
GTGCATGGACATTCAAACAAGGGATGAAAGCCCCACAATGTGCCGGTGTCATCCACTCTGACTTCGAACGTGGATTCATC
CGTGCCGAAGTCGTGTCGTATGAAGACCTTTCAGCAGCAGGAAACATGACGACGGTGAAAGAACAAGGGAAACTTCGTTC
AGAAGGAAAAGAATACGTCTTCCAAGATGGCGACGTTGTCACGTTCCGCTTCAACGTATAA

Upstream 100 bases:

>100_bases
CTATAATTGTGAAGGATGTGACGGTGTGGTGTACTGAATGTACCCACTCCACAGACGGCTTAACATTTCCCGGGAAACAC
CGGATGAGGAGGAACTACTA

Downstream 100 bases:

>100_bases
GTTGATTTACGGAAACGTATTGTAAAAGTTAAGGAAGTTTGATAGTATATTATGACGTGAGTAAAACAATTGTTTGCTCC
TTGCTCCTACAACCGGTAGG

Product: GTP-binding protein YchF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MGLTAGIVGLPNVGKSTLFNAITQAGVEAANYPFATIDPNVGVVEVPDARLDRLTELVKPKKTVPTAFEFTDIAGIVKGA
SKGEGLGNKFLANIREVDAICQVVRCFEDENITHVAGRVSPIDDITTINLELILADLEQVDKRIARVQKMVKAKDKGAVQ
ELEALEIVRDVLESERPARVAELSEDQLAIVKHFQLLTMKPMLYVANVSEDEVADADSNEFVQAVRDHAATEGSKVIVIC
ARIEEEISELEPEERLEFLQDLGIEESGLDQLIRAAYSTLGLATYFTAGEKEVRAWTFKQGMKAPQCAGVIHSDFERGFI
RAEVVSYEDLSAAGNMTTVKEQGKLRSEGKEYVFQDGDVVTFRFNV

Sequences:

>Translated_366_residues
MGLTAGIVGLPNVGKSTLFNAITQAGVEAANYPFATIDPNVGVVEVPDARLDRLTELVKPKKTVPTAFEFTDIAGIVKGA
SKGEGLGNKFLANIREVDAICQVVRCFEDENITHVAGRVSPIDDITTINLELILADLEQVDKRIARVQKMVKAKDKGAVQ
ELEALEIVRDVLESERPARVAELSEDQLAIVKHFQLLTMKPMLYVANVSEDEVADADSNEFVQAVRDHAATEGSKVIVIC
ARIEEEISELEPEERLEFLQDLGIEESGLDQLIRAAYSTLGLATYFTAGEKEVRAWTFKQGMKAPQCAGVIHSDFERGFI
RAEVVSYEDLSAAGNMTTVKEQGKLRSEGKEYVFQDGDVVTFRFNV
>Mature_365_residues
GLTAGIVGLPNVGKSTLFNAITQAGVEAANYPFATIDPNVGVVEVPDARLDRLTELVKPKKTVPTAFEFTDIAGIVKGAS
KGEGLGNKFLANIREVDAICQVVRCFEDENITHVAGRVSPIDDITTINLELILADLEQVDKRIARVQKMVKAKDKGAVQE
LEALEIVRDVLESERPARVAELSEDQLAIVKHFQLLTMKPMLYVANVSEDEVADADSNEFVQAVRDHAATEGSKVIVICA
RIEEEISELEPEERLEFLQDLGIEESGLDQLIRAAYSTLGLATYFTAGEKEVRAWTFKQGMKAPQCAGVIHSDFERGFIR
AEVVSYEDLSAAGNMTTVKEQGKLRSEGKEYVFQDGDVVTFRFNV

Specific function: GTP-dependent nucleic acid-binding protein which may act as a translation factor [H]

COG id: COG0012

COG function: function code J; Predicted GTPase, probable translation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI58761500, Length=369, Percent_Identity=41.7344173441734, Blast_Score=293, Evalue=2e-79,
Organism=Homo sapiens, GI58761502, Length=220, Percent_Identity=35.9090909090909, Blast_Score=149, Evalue=3e-36,
Organism=Escherichia coli, GI1787454, Length=366, Percent_Identity=56.8306010928962, Blast_Score=428, Evalue=1e-121,
Organism=Escherichia coli, GI1789574, Length=141, Percent_Identity=29.7872340425532, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17509631, Length=366, Percent_Identity=40.7103825136612, Blast_Score=288, Evalue=3e-78,
Organism=Saccharomyces cerevisiae, GI6319499, Length=370, Percent_Identity=40.8108108108108, Blast_Score=256, Evalue=4e-69,
Organism=Saccharomyces cerevisiae, GI6321773, Length=382, Percent_Identity=35.8638743455497, Blast_Score=227, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6321649, Length=349, Percent_Identity=26.647564469914, Blast_Score=88, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24640873, Length=367, Percent_Identity=39.2370572207085, Blast_Score=276, Evalue=1e-74,
Organism=Drosophila melanogaster, GI24640877, Length=367, Percent_Identity=39.2370572207085, Blast_Score=276, Evalue=1e-74,
Organism=Drosophila melanogaster, GI24640875, Length=367, Percent_Identity=39.2370572207085, Blast_Score=276, Evalue=1e-74,
Organism=Drosophila melanogaster, GI24640879, Length=326, Percent_Identity=36.8098159509202, Blast_Score=226, Evalue=3e-59,
Organism=Drosophila melanogaster, GI24585318, Length=102, Percent_Identity=34.3137254901961, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR004396
- InterPro:   IPR013029
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR012676
- InterPro:   IPR023192 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF06071 YchF-GTPase_C [H]

EC number: NA

Molecular weight: Translated: 40179; Mature: 40048

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLTAGIVGLPNVGKSTLFNAITQAGVEAANYPFATIDPNVGVVEVPDARLDRLTELVKP
CCCCCCCCCCCCCCHHHHHHHHHHHCCHHCCCCCEEECCCCCEEECCCHHHHHHHHHHCC
KKTVPTAFEFTDIAGIVKGASKGEGLGNKFLANIREVDAICQVVRCFEDENITHVAGRVS
CCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEHHCCCC
PIDDITTINLELILADLEQVDKRIARVQKMVKAKDKGAVQELEALEIVRDVLESERPARV
CCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHH
AELSEDQLAIVKHFQLLTMKPMLYVANVSEDEVADADSNEFVQAVRDHAATEGSKVIVIC
HHCCCHHHHHHHHHHHHHCCCEEEEECCCHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEH
ARIEEEISELEPEERLEFLQDLGIEESGLDQLIRAAYSTLGLATYFTAGEKEVRAWTFKQ
HHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
GMKAPQCAGVIHSDFERGFIRAEVVSYEDLSAAGNMTTVKEQGKLRSEGKEYVFQDGDVV
CCCCCHHHHHHHHHHHCCCHHHEEECHHHHHCCCCCCCHHHHCCHHHCCCCEEEECCCEE
TFRFNV
EEEECC
>Mature Secondary Structure 
GLTAGIVGLPNVGKSTLFNAITQAGVEAANYPFATIDPNVGVVEVPDARLDRLTELVKP
CCCCCCCCCCCCCHHHHHHHHHHHCCHHCCCCCEEECCCCCEEECCCHHHHHHHHHHCC
KKTVPTAFEFTDIAGIVKGASKGEGLGNKFLANIREVDAICQVVRCFEDENITHVAGRVS
CCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEHHCCCC
PIDDITTINLELILADLEQVDKRIARVQKMVKAKDKGAVQELEALEIVRDVLESERPARV
CCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHH
AELSEDQLAIVKHFQLLTMKPMLYVANVSEDEVADADSNEFVQAVRDHAATEGSKVIVIC
HHCCCHHHHHHHHHHHHHCCCEEEEECCCHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEH
ARIEEEISELEPEERLEFLQDLGIEESGLDQLIRAAYSTLGLATYFTAGEKEVRAWTFKQ
HHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
GMKAPQCAGVIHSDFERGFIRAEVVSYEDLSAAGNMTTVKEQGKLRSEGKEYVFQDGDVV
CCCCCHHHHHHHHHHHCCCHHHEEECHHHHHCCCCCCCHHHHCCHHHCCCCEEEECCCEE
TFRFNV
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]