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The map label for this gene is glmU

Identifier: 229917413

GI number: 229917413

Start: 1639839

End: 1641194

Strand: Reverse

Name: glmU

Synonym: EAT1b_1688

Alternate gene names: 229917413

Gene position: 1641194-1639839 (Counterclockwise)

Preceding gene: 229917414

Following gene: 229917412

Centisome position: 54.71

GC content: 51.18

Gene sequence:

>1356_bases
ATGGAGCGTTTTGCAGTCATTTTGGCAGCAGGAAAAGGGACGCGAATGAAGTCGAAGTTATATAAAGTACTTCATCCGGT
TCTAGGGAAACCAATGGTCGAGCATGTTGTCGATCAGTTAGACCAAATCGGTGTCAGTCGTCAAATCGTAATCGTTGGCC
ATGGGGCGGAAGCGGTACAAGACACGCTCGGCACACGGGTAGAGTATGCGGTCCAGGAAGAACAGCTTGGAACAGGGCAC
GCTGTTCAGATGGCTGAAGCAGAGCTTGCGGGTAAATCAGGGGCGACGCTTGTCGTCTGTGGAGATACACCGTTACTCAC
GGCAGAGACGTTAGAAGCCCTTCTCGCGCATCACGAAGCGCAACAAGCAAAAGTCACGGTGTTGACTGCGATTGCTGACG
ATGCGACCGGATATGGTCGAGTCGTTCGTGGTGAAGATGGGAACGTGACGAAAGTCGTGGAACATAAAGATGCTTCAGAG
GCAGAACTCGCCATCAATGAAATCAACACTGGGACGTACGTATTTGATAACGAATTGTTGTTTGATGCACTCAAACAAGT
CGGGAACAACAATGCACAGGGTGAATATTACTTACCTGACGTCATTTCAATCGCAAAAGAAGCGGGCGAAGTGGTTGCGG
CGCATACAGCACCGACGTTTGATGAGACAATTGGGGTCAATGACCGCGTCGCCTTGTCGCAAGCAGAGGCCATCATGCGT
AAACGGACAAATGAACGTCTCATGCGTGAAGGGGTTACCTTCATGGACCCGGCATCGACATACATTTCACCAGATGTGGT
CATCGGTTCGGATACGGTCATTTATCCGGGAACGGTCATTCTTGGCAAGACGACGATTGGCTCAGAATGTGTCATCGGAC
CAAACTCGGACATCCGAAACAGTGTGATTGAAGATCATGCTGTCGTACGCCAGTCTGTCGTGACAGATAGCCGAATCGGT
GAGGCGGCTCAAGTCGGACCGTTCGCTCACTTACGTCAACAAGCTGTCCTCGGTGCGAACACGCGCGTCGGCAACTTTGT
AGAGATTAAAAAATCGACGTTCGGCGACGGCGCGAAAGCGTCACACTTGAGCTATATCGGGGATGCGTCAATCGGTGAGC
GCGTCAACTTAGGTTGTGGCTCGATCACAGTGAACTATGACGGCAAGAACAAGTTCGAGACAGTCGTGGAAGACGATGCG
TTCGTCGGCTGTAACGTCAACTTGATTGCCCCTGTAAAAGTCGGGAAAGGCGCGATTGTCGCTGCTGGATCGACGATTAC
GAGTGATGTACCAGAAGAGGCGCTAGCGATTGCACGCGAACGCCAAACGAATAAAGAAGGCTACACAAAACGATAA

Upstream 100 bases:

>100_bases
GGTTTCGATATAGTAGAAACGGTGTAAATAGGATTTAGTTGCAGAAAAGCCGAACTATACAGACCAATCACAGCTGTTGT
GAGATAGGAGCGAATCAAGC

Downstream 100 bases:

>100_bases
CTTGCTGCCAATCATTATGGAAAACGATGGAGGAGAATCCTAAACATGTCTACTGTATACGAACGAGAAATTCGTCTATT
TAGCTTAAACTCGAACCGCC

Product: UDP-N-acetylglucosamine pyrophosphorylase

Products: NA

Alternate protein names: UDP-N-acetylglucosamine pyrophosphorylase; N-acetylglucosamine-1-phosphate uridyltransferase; Glucosamine-1-phosphate N-acetyltransferase

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MERFAVILAAGKGTRMKSKLYKVLHPVLGKPMVEHVVDQLDQIGVSRQIVIVGHGAEAVQDTLGTRVEYAVQEEQLGTGH
AVQMAEAELAGKSGATLVVCGDTPLLTAETLEALLAHHEAQQAKVTVLTAIADDATGYGRVVRGEDGNVTKVVEHKDASE
AELAINEINTGTYVFDNELLFDALKQVGNNNAQGEYYLPDVISIAKEAGEVVAAHTAPTFDETIGVNDRVALSQAEAIMR
KRTNERLMREGVTFMDPASTYISPDVVIGSDTVIYPGTVILGKTTIGSECVIGPNSDIRNSVIEDHAVVRQSVVTDSRIG
EAAQVGPFAHLRQQAVLGANTRVGNFVEIKKSTFGDGAKASHLSYIGDASIGERVNLGCGSITVNYDGKNKFETVVEDDA
FVGCNVNLIAPVKVGKGAIVAAGSTITSDVPEEALAIARERQTNKEGYTKR

Sequences:

>Translated_451_residues
MERFAVILAAGKGTRMKSKLYKVLHPVLGKPMVEHVVDQLDQIGVSRQIVIVGHGAEAVQDTLGTRVEYAVQEEQLGTGH
AVQMAEAELAGKSGATLVVCGDTPLLTAETLEALLAHHEAQQAKVTVLTAIADDATGYGRVVRGEDGNVTKVVEHKDASE
AELAINEINTGTYVFDNELLFDALKQVGNNNAQGEYYLPDVISIAKEAGEVVAAHTAPTFDETIGVNDRVALSQAEAIMR
KRTNERLMREGVTFMDPASTYISPDVVIGSDTVIYPGTVILGKTTIGSECVIGPNSDIRNSVIEDHAVVRQSVVTDSRIG
EAAQVGPFAHLRQQAVLGANTRVGNFVEIKKSTFGDGAKASHLSYIGDASIGERVNLGCGSITVNYDGKNKFETVVEDDA
FVGCNVNLIAPVKVGKGAIVAAGSTITSDVPEEALAIARERQTNKEGYTKR
>Mature_451_residues
MERFAVILAAGKGTRMKSKLYKVLHPVLGKPMVEHVVDQLDQIGVSRQIVIVGHGAEAVQDTLGTRVEYAVQEEQLGTGH
AVQMAEAELAGKSGATLVVCGDTPLLTAETLEALLAHHEAQQAKVTVLTAIADDATGYGRVVRGEDGNVTKVVEHKDASE
AELAINEINTGTYVFDNELLFDALKQVGNNNAQGEYYLPDVISIAKEAGEVVAAHTAPTFDETIGVNDRVALSQAEAIMR
KRTNERLMREGVTFMDPASTYISPDVVIGSDTVIYPGTVILGKTTIGSECVIGPNSDIRNSVIEDHAVVRQSVVTDSRIG
EAAQVGPFAHLRQQAVLGANTRVGNFVEIKKSTFGDGAKASHLSYIGDASIGERVNLGCGSITVNYDGKNKFETVVEDDA
FVGCNVNLIAPVKVGKGAIVAAGSTITSDVPEEALAIARERQTNKEGYTKR

Specific function: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-GlcNAc. Responsible for the acetylation of Glc-N-1-P to give GlcNAc-1-P and for the uridyl transfer from UTP to GlcNAc-1-P which produces UDP-GlcNAc

COG id: COG1207

COG function: function code M; N-acetylglucosamine-1-phosphate uridyltransferase (contains nucleotidyltransferase and I-patch acetyltransferase domains)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transferase hexapeptide repeat family

Homologues:

Organism=Escherichia coli, GI1790168, Length=448, Percent_Identity=45.0892857142857, Blast_Score=361, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17509979, Length=408, Percent_Identity=27.9411764705882, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17509981, Length=395, Percent_Identity=27.3417721518987, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI133931050, Length=411, Percent_Identity=24.5742092457421, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6320148, Length=379, Percent_Identity=23.7467018469657, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLMU_EXISA (C4KZV1)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002886059.1
- ProteinModelPortal:   C4KZV1
- GeneID:   7869754
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_1688
- OMA:   MERTCLA
- ProtClustDB:   CLSK2488834
- GO:   GO:0005737
- HAMAP:   MF_01631
- InterPro:   IPR005882
- InterPro:   IPR018357
- InterPro:   IPR005835
- InterPro:   IPR011004
- TIGRFAMs:   TIGR01173

Pfam domain/function: PF00483 NTP_transferase; SSF51161 Trimer_LpxA_like

EC number: =2.7.7.23; =2.3.1.157

Molecular weight: Translated: 47952; Mature: 47952

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: ACT_SITE 362-362 BINDING 72-72 BINDING 139-139 BINDING 154-154 BINDING 169-169 BINDING 386-386 BINDING 422-422 BINDING 439-439

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERFAVILAAGKGTRMKSKLYKVLHPVLGKPMVEHVVDQLDQIGVSRQIVIVGHGAEAVQ
CCCEEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEECCHHHHH
DTLGTRVEYAVQEEQLGTGHAVQMAEAELAGKSGATLVVCGDTPLLTAETLEALLAHHEA
HHHCCCEEEEEHHHHCCCCCCEEHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHH
QQAKVTVLTAIADDATGYGRVVRGEDGNVTKVVEHKDASEAELAINEINTGTYVFDNELL
HCCEEEEEEEEECCCCCCCEEEECCCCCEEEEEECCCCCCHHEEEEECCCCEEEECCHHH
FDALKQVGNNNAQGEYYLPDVISIAKEAGEVVAAHTAPTFDETIGVNDRVALSQAEAIMR
HHHHHHHCCCCCCCCEECHHHHHHHHHHCCEEEECCCCCCHHHCCCCCCHHHHHHHHHHH
KRTNERLMREGVTFMDPASTYISPDVVIGSDTVIYPGTVILGKTTIGSECVIGPNSDIRN
HHHHHHHHHCCCCEECCCHHHCCCCEEECCCCEEECCEEEEECCCCCCEEEECCCCHHHH
SVIEDHAVVRQSVVTDSRIGEAAQVGPFAHLRQQAVLGANTRVGNFVEIKKSTFGDGAKA
HHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCC
SHLSYIGDASIGERVNLGCGSITVNYDGKNKFETVVEDDAFVGCNVNLIAPVKVGKGAIV
CHHEECCCCCCCCEEECCCCEEEEEECCCCCHHHEECCCCEEECCEEEEEEEEECCCEEE
AAGSTITSDVPEEALAIARERQTNKEGYTKR
EECCCCCCCCCHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MERFAVILAAGKGTRMKSKLYKVLHPVLGKPMVEHVVDQLDQIGVSRQIVIVGHGAEAVQ
CCCEEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEECCHHHHH
DTLGTRVEYAVQEEQLGTGHAVQMAEAELAGKSGATLVVCGDTPLLTAETLEALLAHHEA
HHHCCCEEEEEHHHHCCCCCCEEHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHH
QQAKVTVLTAIADDATGYGRVVRGEDGNVTKVVEHKDASEAELAINEINTGTYVFDNELL
HCCEEEEEEEEECCCCCCCEEEECCCCCEEEEEECCCCCCHHEEEEECCCCEEEECCHHH
FDALKQVGNNNAQGEYYLPDVISIAKEAGEVVAAHTAPTFDETIGVNDRVALSQAEAIMR
HHHHHHHCCCCCCCCEECHHHHHHHHHHCCEEEECCCCCCHHHCCCCCCHHHHHHHHHHH
KRTNERLMREGVTFMDPASTYISPDVVIGSDTVIYPGTVILGKTTIGSECVIGPNSDIRN
HHHHHHHHHCCCCEECCCHHHCCCCEEECCCCEEECCEEEEECCCCCCEEEECCCCHHHH
SVIEDHAVVRQSVVTDSRIGEAAQVGPFAHLRQQAVLGANTRVGNFVEIKKSTFGDGAKA
HHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCC
SHLSYIGDASIGERVNLGCGSITVNYDGKNKFETVVEDDAFVGCNVNLIAPVKVGKGAIV
CHHEECCCCCCCCEEECCCCEEEEEECCCCCHHHEECCCCEEECCEEEEEEEEECCCEEE
AAGSTITSDVPEEALAIARERQTNKEGYTKR
EECCCCCCCCCHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA