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The map label for this gene is prs [H]

Identifier: 229917412

GI number: 229917412

Start: 1638837

End: 1639793

Strand: Reverse

Name: prs [H]

Synonym: EAT1b_1687

Alternate gene names: 229917412

Gene position: 1639793-1638837 (Counterclockwise)

Preceding gene: 229917413

Following gene: 229917411

Centisome position: 54.66

GC content: 48.38

Gene sequence:

>957_bases
ATGTCTACTGTATACGAACGAGAAATTCGTCTATTTAGCTTAAACTCGAACCGCCCGCTTGCTGAAGAAATCGCGAAAGA
AATTGGTGTTCCGCTCAGCGATTGCCAAGTCAAACGTTTCAGCGACGGTGAACTCTATATCAACATCGAGGAAAGTGTTC
GTGGAGATGATGTGTATGTCATCCAATCAACAAGCTCACCTGTAAACGAAACGCTCATGGAATTACTTGTCATGATCGAT
GCACTCAAACGTGCTTCTGTCCGTACGATCAACATCGTGATGCCGTATTACGGGTATGCACGTCAAGACCGTAAAGCACG
CTCGCGTGAACCGATCACGGCGAAACTTGTCGCTGACCTTCTTACAGTAGCGGGTGCGACACGCGTCATCACGATGGATT
TGCATGCAGCGCAAATTCAAGGATTCTTCAATATTCCGGTTGACCAATTACTTGGTGTACCGCTCATCTCGACTTACTTT
GAAACAGAAGAGTTTCAAGCGAAAGACATCGTGGTCGTTTCTCCAGACCACGGTGGCGTGACACGTGCACGTAAATTGGC
AGAACGTTTGAAAGCGCCGATTGCCATCATTGACAAGCGTCGTCCGAAAGCGAACGTCGCCGAAGTCATGAACATCGTCG
GTAGCGTCGAAGGGAAAACGGCGATTTTGATTGACGACATCATCGATACAGCTGGAACGATCACACTTGCGGCTGATGCT
ATCGTCGAAGCGGGTGCGAAAGAAGTGTATGCGTCTTGTACACACCCAGTTCTTTCTGGTCCAGCGATGGAGCGGATCGA
CAATTCATCAATCAAAGAGTTGGTCGTCTTAAATACCATCGATTTGACGGGTCGCCCTTGCTCGAGCAAGATCAAGCAAA
TGTCGGTCGCCCATTTGCTTGGGGAAGCGATTATGCGCGTACACGAGCATAAATCGGTCAGTACCCTATTCGATTAA

Upstream 100 bases:

>100_bases
GCTAGCGATTGCACGCGAACGCCAAACGAATAAAGAAGGCTACACAAAACGATAACTTGCTGCCAATCATTATGGAAAAC
GATGGAGGAGAATCCTAAAC

Downstream 100 bases:

>100_bases
AAAACGAGGCCCGGAGGCATTCGCCTTTGGGCTTTTTTGTTCAGGAGGAAATGATATGAAATGCATCGTCGGTCTTGGGA
ATCCCGGGAAAAAGTTTGAG

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase [H]

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MSTVYEREIRLFSLNSNRPLAEEIAKEIGVPLSDCQVKRFSDGELYINIEESVRGDDVYVIQSTSSPVNETLMELLVMID
ALKRASVRTINIVMPYYGYARQDRKARSREPITAKLVADLLTVAGATRVITMDLHAAQIQGFFNIPVDQLLGVPLISTYF
ETEEFQAKDIVVVSPDHGGVTRARKLAERLKAPIAIIDKRRPKANVAEVMNIVGSVEGKTAILIDDIIDTAGTITLAADA
IVEAGAKEVYASCTHPVLSGPAMERIDNSSIKELVVLNTIDLTGRPCSSKIKQMSVAHLLGEAIMRVHEHKSVSTLFD

Sequences:

>Translated_318_residues
MSTVYEREIRLFSLNSNRPLAEEIAKEIGVPLSDCQVKRFSDGELYINIEESVRGDDVYVIQSTSSPVNETLMELLVMID
ALKRASVRTINIVMPYYGYARQDRKARSREPITAKLVADLLTVAGATRVITMDLHAAQIQGFFNIPVDQLLGVPLISTYF
ETEEFQAKDIVVVSPDHGGVTRARKLAERLKAPIAIIDKRRPKANVAEVMNIVGSVEGKTAILIDDIIDTAGTITLAADA
IVEAGAKEVYASCTHPVLSGPAMERIDNSSIKELVVLNTIDLTGRPCSSKIKQMSVAHLLGEAIMRVHEHKSVSTLFD
>Mature_317_residues
STVYEREIRLFSLNSNRPLAEEIAKEIGVPLSDCQVKRFSDGELYINIEESVRGDDVYVIQSTSSPVNETLMELLVMIDA
LKRASVRTINIVMPYYGYARQDRKARSREPITAKLVADLLTVAGATRVITMDLHAAQIQGFFNIPVDQLLGVPLISTYFE
TEEFQAKDIVVVSPDHGGVTRARKLAERLKAPIAIIDKRRPKANVAEVMNIVGSVEGKTAILIDDIIDTAGTITLAADAI
VEAGAKEVYASCTHPVLSGPAMERIDNSSIKELVVLNTIDLTGRPCSSKIKQMSVAHLLGEAIMRVHEHKSVSTLFD

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506129, Length=311, Percent_Identity=47.2668810289389, Blast_Score=285, Evalue=6e-77,
Organism=Homo sapiens, GI4506127, Length=312, Percent_Identity=46.7948717948718, Blast_Score=284, Evalue=6e-77,
Organism=Homo sapiens, GI28557709, Length=312, Percent_Identity=46.1538461538462, Blast_Score=283, Evalue=1e-76,
Organism=Homo sapiens, GI84875539, Length=314, Percent_Identity=46.8152866242038, Blast_Score=279, Evalue=3e-75,
Organism=Homo sapiens, GI4506133, Length=343, Percent_Identity=38.1924198250729, Blast_Score=191, Evalue=6e-49,
Organism=Homo sapiens, GI194018537, Length=343, Percent_Identity=37.0262390670554, Blast_Score=180, Evalue=1e-45,
Organism=Homo sapiens, GI310128524, Length=136, Percent_Identity=38.2352941176471, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI310115209, Length=136, Percent_Identity=38.2352941176471, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI310118259, Length=136, Percent_Identity=38.2352941176471, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI310119946, Length=136, Percent_Identity=38.2352941176471, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1787458, Length=314, Percent_Identity=48.7261146496815, Blast_Score=302, Evalue=1e-83,
Organism=Caenorhabditis elegans, GI25149168, Length=311, Percent_Identity=49.1961414790997, Blast_Score=294, Evalue=3e-80,
Organism=Caenorhabditis elegans, GI17554702, Length=311, Percent_Identity=49.1961414790997, Blast_Score=293, Evalue=6e-80,
Organism=Caenorhabditis elegans, GI71989924, Length=311, Percent_Identity=49.1961414790997, Blast_Score=292, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI17554704, Length=309, Percent_Identity=49.1909385113269, Blast_Score=292, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI17570245, Length=336, Percent_Identity=33.9285714285714, Blast_Score=184, Evalue=5e-47,
Organism=Saccharomyces cerevisiae, GI6320946, Length=323, Percent_Identity=46.1300309597523, Blast_Score=263, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6319403, Length=313, Percent_Identity=46.0063897763578, Blast_Score=263, Evalue=3e-71,
Organism=Saccharomyces cerevisiae, GI6321776, Length=316, Percent_Identity=45.253164556962, Blast_Score=256, Evalue=3e-69,
Organism=Saccharomyces cerevisiae, GI6322667, Length=199, Percent_Identity=41.2060301507538, Blast_Score=163, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6324511, Length=113, Percent_Identity=41.5929203539823, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21355239, Length=312, Percent_Identity=48.3974358974359, Blast_Score=288, Evalue=4e-78,
Organism=Drosophila melanogaster, GI45551540, Length=334, Percent_Identity=45.5089820359281, Blast_Score=276, Evalue=1e-74,
Organism=Drosophila melanogaster, GI24651458, Length=362, Percent_Identity=33.4254143646409, Blast_Score=191, Evalue=4e-49,
Organism=Drosophila melanogaster, GI24651456, Length=362, Percent_Identity=33.4254143646409, Blast_Score=191, Evalue=4e-49,
Organism=Drosophila melanogaster, GI281362873, Length=362, Percent_Identity=33.4254143646409, Blast_Score=191, Evalue=5e-49,
Organism=Drosophila melanogaster, GI24651454, Length=362, Percent_Identity=33.4254143646409, Blast_Score=191, Evalue=5e-49,
Organism=Drosophila melanogaster, GI45552010, Length=383, Percent_Identity=30.2872062663185, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24651462, Length=209, Percent_Identity=36.3636363636364, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24651464, Length=209, Percent_Identity=36.3636363636364, Blast_Score=139, Evalue=3e-33,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 34919; Mature: 34788

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00114 PRPP_SYNTHETASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTVYEREIRLFSLNSNRPLAEEIAKEIGVPLSDCQVKRFSDGELYINIEESVRGDDVYV
CCCCEECCEEEEEECCCCCHHHHHHHHHCCCCCCCEEEEECCCEEEEEECCCCCCCEEEE
IQSTSSPVNETLMELLVMIDALKRASVRTINIVMPYYGYARQDRKARSREPITAKLVADL
EECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHH
LTVAGATRVITMDLHAAQIQGFFNIPVDQLLGVPLISTYFETEEFQAKDIVVVSPDHGGV
HHHCCCCEEEEEECHHHHHCCEECCCHHHHHCCHHHHHHHCCCCCCCCEEEEECCCCCCH
TRARKLAERLKAPIAIIDKRRPKANVAEVMNIVGSVEGKTAILIDDIIDTAGTITLAADA
HHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEHHHHCCCCCEEEHHHH
IVEAGAKEVYASCTHPVLSGPAMERIDNSSIKELVVLNTIDLTGRPCSSKIKQMSVAHLL
HHHCCHHHHHHHCCCCHHCCHHHHHCCCCCHHHEEEEEEECCCCCCHHHHHHHHHHHHHH
GEAIMRVHEHKSVSTLFD
HHHHHHHHHCCCHHHHCC
>Mature Secondary Structure 
STVYEREIRLFSLNSNRPLAEEIAKEIGVPLSDCQVKRFSDGELYINIEESVRGDDVYV
CCCEECCEEEEEECCCCCHHHHHHHHHCCCCCCCEEEEECCCEEEEEECCCCCCCEEEE
IQSTSSPVNETLMELLVMIDALKRASVRTINIVMPYYGYARQDRKARSREPITAKLVADL
EECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHH
LTVAGATRVITMDLHAAQIQGFFNIPVDQLLGVPLISTYFETEEFQAKDIVVVSPDHGGV
HHHCCCCEEEEEECHHHHHCCEECCCHHHHHCCHHHHHHHCCCCCCCCEEEEECCCCCCH
TRARKLAERLKAPIAIIDKRRPKANVAEVMNIVGSVEGKTAILIDDIIDTAGTITLAADA
HHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEHHHHCCCCCEEEHHHH
IVEAGAKEVYASCTHPVLSGPAMERIDNSSIKELVVLNTIDLTGRPCSSKIKQMSVAHLL
HHHCCHHHHHHHCCCCHHCCHHHHHCCCCCHHHEEEEEEECCCCCCHHHHHHHHHHHHHH
GEAIMRVHEHKSVSTLFD
HHHHHHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]