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The map label for this gene is pth

Identifier: 229917411

GI number: 229917411

Start: 1638224

End: 1638781

Strand: Reverse

Name: pth

Synonym: EAT1b_1686

Alternate gene names: 229917411

Gene position: 1638781-1638224 (Counterclockwise)

Preceding gene: 229917412

Following gene: 229917410

Centisome position: 54.63

GC content: 45.88

Gene sequence:

>558_bases
ATGAAATGCATCGTCGGTCTTGGGAATCCCGGGAAAAAGTTTGAGATGACGCGACACAATGTCGGGTTTTTAGCGATTGA
CCGTCTCGCCGAAAAGCATGGCATCTCGCTGAATGAAGCGAAGTTTAATGCCCTTATGGGAACGGGAAGAATCAATGGTG
AACGCGTAGTACTCGTCAAACCACTTACGTATATGAACTTGTCGGGTGAAGCCGTCCGTCCCATTTTAGATTATTATAAG
ATTGAGATTGATGATTTATTAGTCATTTATGACGATTTGGATATGGTGCCTGGCAAACTTCGTTTCCGTCCGAAAGGGAG
CGCAGGTGGTCATAATGGGATCAAATCTCTCATTCAACACTTAGGAACGGCTGAATTCAAACGATTAAAGCTCGGAATCG
GTCGTCCCCCGCACCCGATTAAAGTCGTCGACTGGGTGTTGATGAACTATCGCAAAGACGAGTTGCCGGAATTAAATGAA
ACACTCGACAACGCCGTAACTGCAGCCACTGATTTTGTGGACACAGATTGGCTGGCGCTGATGAATCGATACAACTAA

Upstream 100 bases:

>100_bases
ATGCGCGTACACGAGCATAAATCGGTCAGTACCCTATTCGATTAAAAAACGAGGCCCGGAGGCATTCGCCTTTGGGCTTT
TTTGTTCAGGAGGAAATGAT

Downstream 100 bases:

>100_bases
AGGGTCATTGTACCCTTTTTTGTCGTATGAGTTGGAGGAAAACAAATGAATGCTTTAGAGAGATTTATGGTGGCGCTCCC
TGAAACGAATGTGATTCGAG

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MKCIVGLGNPGKKFEMTRHNVGFLAIDRLAEKHGISLNEAKFNALMGTGRINGERVVLVKPLTYMNLSGEAVRPILDYYK
IEIDDLLVIYDDLDMVPGKLRFRPKGSAGGHNGIKSLIQHLGTAEFKRLKLGIGRPPHPIKVVDWVLMNYRKDELPELNE
TLDNAVTAATDFVDTDWLALMNRYN

Sequences:

>Translated_185_residues
MKCIVGLGNPGKKFEMTRHNVGFLAIDRLAEKHGISLNEAKFNALMGTGRINGERVVLVKPLTYMNLSGEAVRPILDYYK
IEIDDLLVIYDDLDMVPGKLRFRPKGSAGGHNGIKSLIQHLGTAEFKRLKLGIGRPPHPIKVVDWVLMNYRKDELPELNE
TLDNAVTAATDFVDTDWLALMNRYN
>Mature_185_residues
MKCIVGLGNPGKKFEMTRHNVGFLAIDRLAEKHGISLNEAKFNALMGTGRINGERVVLVKPLTYMNLSGEAVRPILDYYK
IEIDDLLVIYDDLDMVPGKLRFRPKGSAGGHNGIKSLIQHLGTAEFKRLKLGIGRPPHPIKVVDWVLMNYRKDELPELNE
TLDNAVTAATDFVDTDWLALMNRYN

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family

Homologues:

Organism=Homo sapiens, GI50897284, Length=173, Percent_Identity=31.7919075144509, Blast_Score=91, Evalue=6e-19,
Organism=Escherichia coli, GI1787455, Length=180, Percent_Identity=41.6666666666667, Blast_Score=107, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6321983, Length=184, Percent_Identity=32.6086956521739, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTH_EXISA (C4KZU9)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002886057.1
- GeneID:   7869752
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_1686
- OMA:   AMHRLHS
- ProtClustDB:   PRK05426
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00083
- InterPro:   IPR001328
- InterPro:   IPR018171
- Gene3D:   G3DSA:3.40.50.1470
- PANTHER:   PTHR17224
- TIGRFAMs:   TIGR00447

Pfam domain/function: PF01195 Pept_tRNA_hydro; SSF53178 Pept_tRNA_hydro

EC number: =3.1.1.29

Molecular weight: Translated: 20790; Mature: 20790

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKCIVGLGNPGKKFEMTRHNVGFLAIDRLAEKHGISLNEAKFNALMGTGRINGERVVLVK
CEEEEECCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHEEECCCCCCCEEEEEC
PLTYMNLSGEAVRPILDYYKIEIDDLLVIYDDLDMVPGKLRFRPKGSAGGHNGIKSLIQH
CEEEECCCCHHHHHHHHHHEEEECCEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHH
LGTAEFKRLKLGIGRPPHPIKVVDWVLMNYRKDELPELNETLDNAVTAATDFVDTDWLAL
CCCHHHHHHEECCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHH
MNRYN
HHCCC
>Mature Secondary Structure
MKCIVGLGNPGKKFEMTRHNVGFLAIDRLAEKHGISLNEAKFNALMGTGRINGERVVLVK
CEEEEECCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHEEECCCCCCCEEEEEC
PLTYMNLSGEAVRPILDYYKIEIDDLLVIYDDLDMVPGKLRFRPKGSAGGHNGIKSLIQH
CEEEECCCCHHHHHHHHHHEEEECCEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHH
LGTAEFKRLKLGIGRPPHPIKVVDWVLMNYRKDELPELNETLDNAVTAATDFVDTDWLAL
CCCHHHHHHEECCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHH
MNRYN
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA