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The map label for this gene is gltX [H]

Identifier: 229917381

GI number: 229917381

Start: 1598269

End: 1599723

Strand: Reverse

Name: gltX [H]

Synonym: EAT1b_1656

Alternate gene names: 229917381

Gene position: 1599723-1598269 (Counterclockwise)

Preceding gene: 229917382

Following gene: 229917380

Centisome position: 53.33

GC content: 48.18

Gene sequence:

>1455_bases
ATGAGTGAAGTGCGTGTACGCTATGCGCCTAGTCCGACGGGGCATCTCCATATCGGAAATGCGCGGACTGCGTTGTTTAA
CTACTTATTTGCTCGCCATGCTGGCGGAAAAATGATTCTTCGGATTGAAGACACTGACCAGAAACGGAATGTGTTGAACG
GTGTCGAGAGTCAGATGAAATACTTGGAATGGCTCGGCATCGACTGGGATGAAGGTCCAGGTCGTGACGGAGACTACGGC
CCATATTATCAAATGGAGCGTCTTGCGATTTATGAAAAATACGTCAACGAGTTGATGAGCAAAGGCCTCGCTTATAAATG
CTACATGACTTCTGAGGAGCTTGAAGCAGAGCGTGAAGCACAAATCGCACGTGGGGAGGCACCACGTTATTCGGGTGCGC
ACCGTAACCTCACTGTTGAAGAGCGTGAAGCGTTTGAAGCGGAAGGGCGTAAACCGTCTATTCGGATCCGTGTGCCGGAA
GGCGTGACGTATAAATGGACAGATGTCGTCAAAGGAGACGTCTCGTTTGAATCAAAAGATTTCGGTGACTGGGTCATCGT
GAAACAGGATGGCATTCCGACGTACAACTTCGCAGTTGTCGTGGACGACCATTTGATGAAGATCAGTCACGTGTTGCGTG
GGGACGATCACATCGCGAACACACCGAAACAGATGATGGTCTATGATGCACTCGGTTGGGACTATCCTGAATTCGGTCAC
ATGACGCTCATCTACAATGACCAACATAAGAAGTTATCAAAACGGGACGAATCGATTATTCAGTTCATCGAACAATATGC
GGATCTCGGCTACTTGCCAGCAGCACTCTTTAACTTCATTTCGCTCCTTGGCTGGTCACCGGTCGGGGAAGAAGAAATCT
TCTCAAAAGAAGAGTTCATTGAAATGTTTGATGCGAAACGTTTGTCGAAGAGTCCGGCAGTTTTTGACCAACAAAAACTT
TCTTGGATCAACAGTGTATACATGAAGAAGGCCACGCTTGACGAAGTGGTGGCACTCAGTTTACCATTCTTACAAGAAGC
AGGACGATTACCCGAGGCGGTATCTGTTGAGGAAGCGGATTGGGCGACAAACTTGATTGCATTGTATAAAGAACAAATGA
CGCACGGAGCAGAAATCGTCGCGTTGACAGACTTGTTCTTCAAAGATGAAATCGAGTATGACGAGGAAGCGAACGCTGTC
CTTTCAGGTGAAACGGTACCTGCTGTTCTTGCAGAGTTCAAACAGCAGCTCGAAGCAATCGAAGACTTCACACCGGAAGC
GATTAAAGCGGCGACGAAAGCGACGCAAAAAGCGACCGGTCAAAAAGGGAAGAACTTGTTCATGCCGATCCGTGTCGCTA
CGACAGGGCAAACACATGGACCGGAACTCCCGAACGCCATTTCGTTAATCGGAAAAGAGCGTGTGTTGAAGCGTCTGACA
ACTTTATTAGCGTAA

Upstream 100 bases:

>100_bases
ACGCGAAGAGGGAATCGCGGCGCAAGCGGTCATCTTACTCAACCGGGCTTGACAGAAATGGTATACTATTTGGGCAGAAG
AGAAGAAAGGTGGAACTATT

Downstream 100 bases:

>100_bases
TAAAGGCATCGATGAGGAAAGTAAGCGGTACGACACGTTGCAGAGACTAGAACCTAGGCTGGGAGTTCTAGATGTGTCCC
CCGAAGAAGTGCACCTCGGA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MSEVRVRYAPSPTGHLHIGNARTALFNYLFARHAGGKMILRIEDTDQKRNVLNGVESQMKYLEWLGIDWDEGPGRDGDYG
PYYQMERLAIYEKYVNELMSKGLAYKCYMTSEELEAEREAQIARGEAPRYSGAHRNLTVEEREAFEAEGRKPSIRIRVPE
GVTYKWTDVVKGDVSFESKDFGDWVIVKQDGIPTYNFAVVVDDHLMKISHVLRGDDHIANTPKQMMVYDALGWDYPEFGH
MTLIYNDQHKKLSKRDESIIQFIEQYADLGYLPAALFNFISLLGWSPVGEEEIFSKEEFIEMFDAKRLSKSPAVFDQQKL
SWINSVYMKKATLDEVVALSLPFLQEAGRLPEAVSVEEADWATNLIALYKEQMTHGAEIVALTDLFFKDEIEYDEEANAV
LSGETVPAVLAEFKQQLEAIEDFTPEAIKAATKATQKATGQKGKNLFMPIRVATTGQTHGPELPNAISLIGKERVLKRLT
TLLA

Sequences:

>Translated_484_residues
MSEVRVRYAPSPTGHLHIGNARTALFNYLFARHAGGKMILRIEDTDQKRNVLNGVESQMKYLEWLGIDWDEGPGRDGDYG
PYYQMERLAIYEKYVNELMSKGLAYKCYMTSEELEAEREAQIARGEAPRYSGAHRNLTVEEREAFEAEGRKPSIRIRVPE
GVTYKWTDVVKGDVSFESKDFGDWVIVKQDGIPTYNFAVVVDDHLMKISHVLRGDDHIANTPKQMMVYDALGWDYPEFGH
MTLIYNDQHKKLSKRDESIIQFIEQYADLGYLPAALFNFISLLGWSPVGEEEIFSKEEFIEMFDAKRLSKSPAVFDQQKL
SWINSVYMKKATLDEVVALSLPFLQEAGRLPEAVSVEEADWATNLIALYKEQMTHGAEIVALTDLFFKDEIEYDEEANAV
LSGETVPAVLAEFKQQLEAIEDFTPEAIKAATKATQKATGQKGKNLFMPIRVATTGQTHGPELPNAISLIGKERVLKRLT
TLLA
>Mature_483_residues
SEVRVRYAPSPTGHLHIGNARTALFNYLFARHAGGKMILRIEDTDQKRNVLNGVESQMKYLEWLGIDWDEGPGRDGDYGP
YYQMERLAIYEKYVNELMSKGLAYKCYMTSEELEAEREAQIARGEAPRYSGAHRNLTVEEREAFEAEGRKPSIRIRVPEG
VTYKWTDVVKGDVSFESKDFGDWVIVKQDGIPTYNFAVVVDDHLMKISHVLRGDDHIANTPKQMMVYDALGWDYPEFGHM
TLIYNDQHKKLSKRDESIIQFIEQYADLGYLPAALFNFISLLGWSPVGEEEIFSKEEFIEMFDAKRLSKSPAVFDQQKLS
WINSVYMKKATLDEVVALSLPFLQEAGRLPEAVSVEEADWATNLIALYKEQMTHGAEIVALTDLFFKDEIEYDEEANAVL
SGETVPAVLAEFKQQLEAIEDFTPEAIKAATKATQKATGQKGKNLFMPIRVATTGQTHGPELPNAISLIGKERVLKRLTT
LLA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=502, Percent_Identity=33.0677290836653, Blast_Score=252, Evalue=6e-67,
Organism=Escherichia coli, GI1788743, Length=481, Percent_Identity=37.6299376299376, Blast_Score=325, Evalue=3e-90,
Organism=Escherichia coli, GI226510925, Length=253, Percent_Identity=33.596837944664, Blast_Score=129, Evalue=6e-31,
Organism=Escherichia coli, GI1786895, Length=221, Percent_Identity=29.4117647058824, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71993394, Length=487, Percent_Identity=32.4435318275154, Blast_Score=233, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6324540, Length=509, Percent_Identity=33.2023575638507, Blast_Score=244, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6321192, Length=216, Percent_Identity=27.7777777777778, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324742, Length=143, Percent_Identity=31.4685314685315, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24665321, Length=496, Percent_Identity=33.8709677419355, Blast_Score=267, Evalue=1e-71,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 54891; Mature: 54760

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEVRVRYAPSPTGHLHIGNARTALFNYLFARHAGGKMILRIEDTDQKRNVLNGVESQMK
CCCCEEEECCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHH
YLEWLGIDWDEGPGRDGDYGPYYQMERLAIYEKYVNELMSKGLAYKCYMTSEELEAEREA
HHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHH
QIARGEAPRYSGAHRNLTVEEREAFEAEGRKPSIRIRVPEGVTYKWTDVVKGDVSFESKD
HHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCEEEEHHHEECCCCCCCCC
FGDWVIVKQDGIPTYNFAVVVDDHLMKISHVLRGDDHIANTPKQMMVYDALGWDYPEFGH
CCCEEEEECCCCCCEEEEEEECHHHHHHHHHHCCCCHHCCCCHHHEEEHHCCCCCCCCCC
MTLIYNDQHKKLSKRDESIIQFIEQYADLGYLPAALFNFISLLGWSPVGEEEIFSKEEFI
EEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
EMFDAKRLSKSPAVFDQQKLSWINSVYMKKATLDEVVALSLPFLQEAGRLPEAVSVEEAD
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
WATNLIALYKEQMTHGAEIVALTDLFFKDEIEYDEEANAVLSGETVPAVLAEFKQQLEAI
HHHHHHHHHHHHHCCCCEEEEEHHHHHHCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHH
EDFTPEAIKAATKATQKATGQKGKNLFMPIRVATTGQTHGPELPNAISLIGKERVLKRLT
HHCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHH
TLLA
HHHC
>Mature Secondary Structure 
SEVRVRYAPSPTGHLHIGNARTALFNYLFARHAGGKMILRIEDTDQKRNVLNGVESQMK
CCCEEEECCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHH
YLEWLGIDWDEGPGRDGDYGPYYQMERLAIYEKYVNELMSKGLAYKCYMTSEELEAEREA
HHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHH
QIARGEAPRYSGAHRNLTVEEREAFEAEGRKPSIRIRVPEGVTYKWTDVVKGDVSFESKD
HHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCEEEEHHHEECCCCCCCCC
FGDWVIVKQDGIPTYNFAVVVDDHLMKISHVLRGDDHIANTPKQMMVYDALGWDYPEFGH
CCCEEEEECCCCCCEEEEEEECHHHHHHHHHHCCCCHHCCCCHHHEEEHHCCCCCCCCCC
MTLIYNDQHKKLSKRDESIIQFIEQYADLGYLPAALFNFISLLGWSPVGEEEIFSKEEFI
EEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
EMFDAKRLSKSPAVFDQQKLSWINSVYMKKATLDEVVALSLPFLQEAGRLPEAVSVEEAD
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
WATNLIALYKEQMTHGAEIVALTDLFFKDEIEYDEEANAVLSGETVPAVLAEFKQQLEAI
HHHHHHHHHHHHHCCCCEEEEEHHHHHHCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHH
EDFTPEAIKAATKATQKATGQKGKNLFMPIRVATTGQTHGPELPNAISLIGKERVLKRLT
HHCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHH
TLLA
HHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA