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The map label for this gene is ywtE [H]

Identifier: 229917227

GI number: 229917227

Start: 1467828

End: 1468676

Strand: Reverse

Name: ywtE [H]

Synonym: EAT1b_1502

Alternate gene names: 229917227

Gene position: 1468676-1467828 (Counterclockwise)

Preceding gene: 229917233

Following gene: 229917226

Centisome position: 48.96

GC content: 46.64

Gene sequence:

>849_bases
ATGAAATTGATTGCGATTGATTTAGACGGGACGCTGTTATCGCGCACGGGCGTGATAACAAAGGCAAACCAGGAGGCGAT
TCGTCGTCGGCAAGAACAGGGTGATATCGTCACGATTTGTTCAGGACGTTCGATTCATGATATCGAGGAGCTGTTGAAGC
GAAGTGACCTCGTCTGTCCGATTTTGTCAGGTAACGGAGCAATCGGGTTTTATGAGAACGAGCGAATTTACTATCATGCG
ATGGATCAAACGGTTGTAGACGAGCTATGGGAAATCGCGAACGCAGATGATGCGTACGTCGAATTTTATACGAATACGGG
AGTGAAAGTACTCCGTTCAGGGAGTGATTTATTAAAACGGGAACTCAATGAGGTGTTGCCACATGATCAATCGTTCACCC
GTGAGTGGGCGGAACGTGAGATTGAAATCCAAAATGAGCAGTTCGGTCTGACATATATGGATTCGATGGCAGACATCGAC
TTTGAACGAGATGAAGTGTACAAGGTGTTTGTCTATTCGTTCGATCGCCGAAAGCTACACCGGTTGCGTAAAATCTATGA
ACCACGGAACGATATTTTGATTACGACTGCGGGGTGGACGAAAATTGAAATCGGACATCCTCATGCGAGTAAAGGCGCAG
CCTTGAAACAGCTAGCTGAGCATTATCAGATTCCGATGCGTGATGTCGTCGCCATCGGAGACAATCTGAATGATGTGTCG
ATGTTCGAAGTGGCAGAAACAAGGATTGCGATGGGGAATGCTGTGGATCCGTTAAAAGATTTGAGCACGCATATTACAAA
GGATGTAGAAGAGGACGGGGTCGCTTATGCGCTGACGCATTATGTATGA

Upstream 100 bases:

>100_bases
AACAAACAACGAGGGCAGGTAGACATCATACACATTGGTATTCCCTTCATCGTGTTTTACGCAGTTGAATCGGGAAAAGA
ACTAAGGAGGAGGGAACGAT

Downstream 100 bases:

>100_bases
ATTGGATTATGGAGAAATCGTGAACGAGGATTGAATCTCACTTAAAACGGATATTGTATACATGTAACCATTCACTTTCT
GAAATGAGTCGGGGAACGGG

Product: Cof-like hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MKLIAIDLDGTLLSRTGVITKANQEAIRRRQEQGDIVTICSGRSIHDIEELLKRSDLVCPILSGNGAIGFYENERIYYHA
MDQTVVDELWEIANADDAYVEFYTNTGVKVLRSGSDLLKRELNEVLPHDQSFTREWAEREIEIQNEQFGLTYMDSMADID
FERDEVYKVFVYSFDRRKLHRLRKIYEPRNDILITTAGWTKIEIGHPHASKGAALKQLAEHYQIPMRDVVAIGDNLNDVS
MFEVAETRIAMGNAVDPLKDLSTHITKDVEEDGVAYALTHYV

Sequences:

>Translated_282_residues
MKLIAIDLDGTLLSRTGVITKANQEAIRRRQEQGDIVTICSGRSIHDIEELLKRSDLVCPILSGNGAIGFYENERIYYHA
MDQTVVDELWEIANADDAYVEFYTNTGVKVLRSGSDLLKRELNEVLPHDQSFTREWAEREIEIQNEQFGLTYMDSMADID
FERDEVYKVFVYSFDRRKLHRLRKIYEPRNDILITTAGWTKIEIGHPHASKGAALKQLAEHYQIPMRDVVAIGDNLNDVS
MFEVAETRIAMGNAVDPLKDLSTHITKDVEEDGVAYALTHYV
>Mature_282_residues
MKLIAIDLDGTLLSRTGVITKANQEAIRRRQEQGDIVTICSGRSIHDIEELLKRSDLVCPILSGNGAIGFYENERIYYHA
MDQTVVDELWEIANADDAYVEFYTNTGVKVLRSGSDLLKRELNEVLPHDQSFTREWAEREIEIQNEQFGLTYMDSMADID
FERDEVYKVFVYSFDRRKLHRLRKIYEPRNDILITTAGWTKIEIGHPHASKGAALKQLAEHYQIPMRDVVAIGDNLNDVS
MFEVAETRIAMGNAVDPLKDLSTHITKDVEEDGVAYALTHYV

Specific function: Unknown

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family [H]

Homologues:

Organism=Escherichia coli, GI1786982, Length=276, Percent_Identity=26.0869565217391, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI2367265, Length=81, Percent_Identity=39.5061728395062, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 32256; Mature: 32256

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS01228 COF_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIAIDLDGTLLSRTGVITKANQEAIRRRQEQGDIVTICSGRSIHDIEELLKRSDLVCP
CEEEEEECCCCCHHCCCCEEECCHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCEEE
ILSGNGAIGFYENERIYYHAMDQTVVDELWEIANADDAYVEFYTNTGVKVLRSGSDLLKR
EECCCCEEEEEECCEEEEEECCHHHHHHHHHHCCCCCCEEEEEECCCHHHHHCCHHHHHH
ELNEVLPHDQSFTREWAEREIEIQNEQFGLTYMDSMADIDFERDEVYKVFVYSFDRRKLH
HHHHHCCCCHHHHHHHHHHCEEECCCCCCCEEHHHHHCCCCCHHHEEEEEEEECCHHHHH
RLRKIYEPRNDILITTAGWTKIEIGHPHASKGAALKQLAEHYQIPMRDVVAIGDNLNDVS
HHHHHHCCCCCEEEEECCCEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHEECCCCCCHH
MFEVAETRIAMGNAVDPLKDLSTHITKDVEEDGVAYALTHYV
HHHHHHHHHHCCCCCCHHHHHHHHHHHCHHHCCCEEEEEECC
>Mature Secondary Structure
MKLIAIDLDGTLLSRTGVITKANQEAIRRRQEQGDIVTICSGRSIHDIEELLKRSDLVCP
CEEEEEECCCCCHHCCCCEEECCHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCEEE
ILSGNGAIGFYENERIYYHAMDQTVVDELWEIANADDAYVEFYTNTGVKVLRSGSDLLKR
EECCCCEEEEEECCEEEEEECCHHHHHHHHHHCCCCCCEEEEEECCCHHHHHCCHHHHHH
ELNEVLPHDQSFTREWAEREIEIQNEQFGLTYMDSMADIDFERDEVYKVFVYSFDRRKLH
HHHHHCCCCHHHHHHHHHHCEEECCCCCCCEEHHHHHCCCCCHHHEEEEEEEECCHHHHH
RLRKIYEPRNDILITTAGWTKIEIGHPHASKGAALKQLAEHYQIPMRDVVAIGDNLNDVS
HHHHHHCCCCCEEEEECCCEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHEECCCCCCHH
MFEVAETRIAMGNAVDPLKDLSTHITKDVEEDGVAYALTHYV
HHHHHHHHHHCCCCCCHHHHHHHHHHHCHHHCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]