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The map label for this gene is sigK [H]

Identifier: 229917220

GI number: 229917220

Start: 1461509

End: 1462069

Strand: Direct

Name: sigK [H]

Synonym: EAT1b_1495

Alternate gene names: 229917220

Gene position: 1461509-1462069 (Clockwise)

Preceding gene: 229917219

Following gene: 229917221

Centisome position: 48.72

GC content: 46.35

Gene sequence:

>561_bases
ATGACTGACTTAGAGTTATATGAGCGTGTCAGGCAAAAGGACAAAGAAGCGCTCGAAATATTGTATGATCGATACGAACG
CATTTTATACGCATTTGTCCTACAACTGACAAAAGATCGTGATTTGGCCGAAGAAGCCATGCAAGAAGTATTCATCAAGC
TTTGGCGTGGCGGAGGCGTTTACGATGAGTCAAAAGGAAAATTTCGTTCTTGGCTCTTCACAATGAGCCGTCATGTGGCC
ATTGATTTAATTCGGAAGCGTAAACCGGATTTACAATTAGATGAATCTTACGCTGAGGCCATCCCTTCCGACTCACCGTC
CGTCGAGAAAGAAGTCGAGTGGCAAGAAGAGCGCGCCCAAATCGAGACAGCCATGGCCACATTACCAGCCGAACAACAGC
AGGTCGTTCGACTCATGTATTTTCAAGGATTGAGCCAGCAAAAAATTGCTGATGCATGCGGCATCCCGCTCGGAACGGTA
AAAGGAAGGATTCGACTGGCGCTAAGGCAACTGAGGAAACAGTTATCCCATGAATTCATCGGAAAGGAGGTAGAACAATG
A

Upstream 100 bases:

>100_bases
GACATGTTCAATAAATAGTCACACAAAATAGGCATCCATTTTTCGATTTTATACGTTAGATCAATACAAGGGACATCCCT
TCTGCGCAAAGGAGTATGTT

Downstream 100 bases:

>100_bases
ACGCATGCGACCAATTGATTGACTACTTCAACCAACATTTAACGGAAGAAGAACGAATGAAATTTGAACAACATCTGGCA
ACGTGTAAAGACTGTCAAGA

Product: RNA polymerase, sigma-24 subunit, ECF subfamily

Products: NA

Alternate protein names: Sigma-K factor [H]

Number of amino acids: Translated: 186; Mature: 185

Protein sequence:

>186_residues
MTDLELYERVRQKDKEALEILYDRYERILYAFVLQLTKDRDLAEEAMQEVFIKLWRGGGVYDESKGKFRSWLFTMSRHVA
IDLIRKRKPDLQLDESYAEAIPSDSPSVEKEVEWQEERAQIETAMATLPAEQQQVVRLMYFQGLSQQKIADACGIPLGTV
KGRIRLALRQLRKQLSHEFIGKEVEQ

Sequences:

>Translated_186_residues
MTDLELYERVRQKDKEALEILYDRYERILYAFVLQLTKDRDLAEEAMQEVFIKLWRGGGVYDESKGKFRSWLFTMSRHVA
IDLIRKRKPDLQLDESYAEAIPSDSPSVEKEVEWQEERAQIETAMATLPAEQQQVVRLMYFQGLSQQKIADACGIPLGTV
KGRIRLALRQLRKQLSHEFIGKEVEQ
>Mature_185_residues
TDLELYERVRQKDKEALEILYDRYERILYAFVLQLTKDRDLAEEAMQEVFIKLWRGGGVYDESKGKFRSWLFTMSRHVAI
DLIRKRKPDLQLDESYAEAIPSDSPSVEKEVEWQEERAQIETAMATLPAEQQQVVRLMYFQGLSQQKIADACGIPLGTVK
GRIRLALRQLRKQLSHEFIGKEVEQ

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 21796; Mature: 21665

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLELYERVRQKDKEALEILYDRYERILYAFVLQLTKDRDLAEEAMQEVFIKLWRGGGV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
YDESKGKFRSWLFTMSRHVAIDLIRKRKPDLQLDESYAEAIPSDSPSVEKEVEWQEERAQ
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHH
IETAMATLPAEQQQVVRLMYFQGLSQQKIADACGIPLGTVKGRIRLALRQLRKQLSHEFI
HHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
GKEVEQ
HHHCCC
>Mature Secondary Structure 
TDLELYERVRQKDKEALEILYDRYERILYAFVLQLTKDRDLAEEAMQEVFIKLWRGGGV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
YDESKGKFRSWLFTMSRHVAIDLIRKRKPDLQLDESYAEAIPSDSPSVEKEVEWQEERAQ
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHH
IETAMATLPAEQQQVVRLMYFQGLSQQKIADACGIPLGTVKGRIRLALRQLRKQLSHEFI
HHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
GKEVEQ
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA