Click here to switch to the map view.

The map label for this gene is ytwF [H]

Identifier: 229917169

GI number: 229917169

Start: 1409824

End: 1410141

Strand: Reverse

Name: ytwF [H]

Synonym: EAT1b_1443

Alternate gene names: 229917169

Gene position: 1410141-1409824 (Counterclockwise)

Preceding gene: 229917170

Following gene: 229917168

Centisome position: 47.01

GC content: 47.8

Gene sequence:

>318_bases
TTGAAAGGGGAACTATGTATGCAAAGCATCCATGCGACAGAATTAAGAGAAAAATTAGAGAACGGGGAGTCTCTCCATAT
CATCGATGTGCGCGAGCAAGATGAATATGAGGCAGGGCATATCCCGAACGTACCACTCTACCCATTATCGGAATTCCCAC
AAGTGACAGAAAAGTTGGCACAAGATAATGTCTATCACGTCATCTGTCGTTCAGGTGGACGGAGCCAGACGGCGTGTGAT
TACTTAGAATCGCAAGGTTATAAAGTCGTCAATGTTGACGGCGGAATGCTCGCTTGGGACGGCGACATCGAGGCGTGA

Upstream 100 bases:

>100_bases
CGCAACGAAGCGTAAGTCCGGAGCATCCTTCTTATAGAAGGGTGCTTTTTTTGTGTTTTTTTGAATGGTAGCTGAGGTAA
ACTAAAAGAGGAATCCATGA

Downstream 100 bases:

>100_bases
TGCTAGAGAATCCTTGGGTCGTCGTACCGCTTCTCATTGCGCTCTACCTCACGGTCTATGCAGTGATGAAGCGATTTGAC
TGGGAGAAGTGGCGACTCGT

Product: Rhodanese domain protein

Products: H+; Sulfite; Thiocyanate [C]

Alternate protein names: NA

Number of amino acids: Translated: 105; Mature: 105

Protein sequence:

>105_residues
MKGELCMQSIHATELREKLENGESLHIIDVREQDEYEAGHIPNVPLYPLSEFPQVTEKLAQDNVYHVICRSGGRSQTACD
YLESQGYKVVNVDGGMLAWDGDIEA

Sequences:

>Translated_105_residues
MKGELCMQSIHATELREKLENGESLHIIDVREQDEYEAGHIPNVPLYPLSEFPQVTEKLAQDNVYHVICRSGGRSQTACD
YLESQGYKVVNVDGGMLAWDGDIEA
>Mature_105_residues
MKGELCMQSIHATELREKLENGESLHIIDVREQDEYEAGHIPNVPLYPLSEFPQVTEKLAQDNVYHVICRSGGRSQTACD
YLESQGYKVVNVDGGMLAWDGDIEA

Specific function: Catalyzes, Although With Low Efficiency, The Sulfur Transfer Reaction From Thiosulfate To Cyanide. The Relatively Low Affinity Of Glpe For Both Thiosulfate And Cyanide Suggests That These Compounds Are Not The Physiological Substrates. Thioredoxin 1 Or Re

COG id: COG0607

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: 2.8.1.1 [C]

Molecular weight: Translated: 11781; Mature: 11781

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGELCMQSIHATELREKLENGESLHIIDVREQDEYEAGHIPNVPLYPLSEFPQVTEKLA
CCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
QDNVYHVICRSGGRSQTACDYLESQGYKVVNVDGGMLAWDGDIEA
CCCEEEEEECCCCCCCHHHHHHHHCCCEEEEECCCEEEECCCCCC
>Mature Secondary Structure
MKGELCMQSIHATELREKLENGESLHIIDVREQDEYEAGHIPNVPLYPLSEFPQVTEKLA
CCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
QDNVYHVICRSGGRSQTACDYLESQGYKVVNVDGGMLAWDGDIEA
CCCEEEEEECCCCCCCHHHHHHHHCCCEEEEECCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cyanide; Thiosulfate [C]

Specific reaction: Cyanide + Thiosulfate --> H+ + Sulfite + Thiocyanate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]