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The map label for this gene is cls1 [H]

Identifier: 229917113

GI number: 229917113

Start: 1357957

End: 1359477

Strand: Direct

Name: cls1 [H]

Synonym: EAT1b_1387

Alternate gene names: 229917113

Gene position: 1357957-1359477 (Clockwise)

Preceding gene: 229917112

Following gene: 229917123

Centisome position: 45.27

GC content: 48.39

Gene sequence:

>1521_bases
TTGAAACGTCGCGTGATTCAACTGATCATCATGGTCATCAGTGTCATCTTGCTTATCGGTCTTTTGTTTTGGCTTGATGT
CCTCATACCCGTACTAATCGGGATTGCCATCGTCGCACCGCTCGTCGTCGGATGGCAGATTTTATTTGACCAAGTACAAC
CTCAAGGAAAGCTCGCCTGGTTACTGACCGTCTTCTTTATTCCGTTCATCGGCGTCTTGGCATGGGTCATGTTCGGACGC
ACTCCCCGCCGTCATCGACGCTTCAAACGGACGTCATCAGAGATTCGGATGCTACGGCAAGCAATCGAAAGTGAGAAAAT
CGAGACCTCACCTGACGTCGTGTCCTCTTTTCCGTTGACACGGACACTCGAAAAACTAGGGGCCACGGGTGCGGACGCTC
ATACCTCGACTAAACTGCTCGTCAACGGGGAAGAAAAATTCGATGCATTGTTAGACGCCATCAACTCTGCAACCGATCAC
ATCCACATCCAATACTATCTCTTTCGGACGGATGACATTTCGTTAAAAATTAGAGACGCCCTTATCCAAAAATCGAATGA
AAATGTCATTGTCCGCTTTCTGTATGACGGGCTTGGCAGTCAAGAAATCGGGGAAGAGTTCATCCAACCGTTACGGGAGT
CCCGTGTCCACGTTCAGGCGTTTGACCCTGTCTTACATCCATACCTCGTGTTTAACGCCAACTTCCGCAACCATCAAAAA
CTGATTGTCGTGGATGGGCGAATCGCGTTAACAGGCGGGTTGAACGTCGGGGATGAATATCTTGGGAAGAATGATAAACT
CGGTTTCTGGCGTGACACCCACCTCCGTCTCGAAGGTCCGCTCGTCCGAGAATTACAGCAGCTCTTTGTCGAGAACTGGC
TATACGCAGGACAAGGGACGAGTGATGAGACGTGGGATCTGTTCGCAGATGAAAAGCACCTCTCTCAGTACTTTGTCAAT
GAACCAAAAGGGCAAAACGGAGCGACACAACTACTTGTCACATCACCTGGTAAAGACGTCACGATTCGCGATGGACTCAT
GCGTCTGTTGACGGATGCCAAAGAATCAATTTGGCTCACCACCCCATACCTCGTCCCTCCACCTGAATTGTTGGCGATTC
TCGAAGTCGCAGGTCGTAGCGGCATCGATGTCCGAATCGTCGTACCGGGCAAAGGAGACGAATGGTTCAGCTTTCATGCG
ACCGAGTATTATTTTGAACAACTGTTGAAGCGCAATATCAAAATTTATAAATACAACCGCCATTTCATCCATGCGAAAAC
GCTCCTGATTGATGGTAAAGTTGCCCTCGTCGGATCAGCCAACTTCGACTTTCGTAGTATGTTTCTCAACCACGAGATGA
TGATTGCCCAATTCGATACTACATCCATTCAAGAACTGCACGAGGCATTTCTAAAGGACTTTGATGAATCCATTTTAATC
GATTGGACCATGCTGCGGAAAAAGACGACCGGCAAACGTTTCGTCGAAGCGTTTTGTCACATTTTCTCGCCACTTCTTTG
A

Upstream 100 bases:

>100_bases
TTATGCGGACGAAATCAACGCTGTCTTGAACAGCGTGTACTAGAAAAACGAGGGGGCATCTCCCCTCGTTTTCATCGTTA
CTGGAAAGGTGGTGTTTCGC

Downstream 100 bases:

>100_bases
CGCATTCGCCACACAAGATACGTCATGGCGACGACGCCAACTAAAAACAGCTGCCATCCAATCGTCAACTGCATCGCTGC
CGTCACCGCCAGTCCTTCCA

Product: phospholipase D/transphosphatidylase

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: CL synthase 1 [H]

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MKRRVIQLIIMVISVILLIGLLFWLDVLIPVLIGIAIVAPLVVGWQILFDQVQPQGKLAWLLTVFFIPFIGVLAWVMFGR
TPRRHRRFKRTSSEIRMLRQAIESEKIETSPDVVSSFPLTRTLEKLGATGADAHTSTKLLVNGEEKFDALLDAINSATDH
IHIQYYLFRTDDISLKIRDALIQKSNENVIVRFLYDGLGSQEIGEEFIQPLRESRVHVQAFDPVLHPYLVFNANFRNHQK
LIVVDGRIALTGGLNVGDEYLGKNDKLGFWRDTHLRLEGPLVRELQQLFVENWLYAGQGTSDETWDLFADEKHLSQYFVN
EPKGQNGATQLLVTSPGKDVTIRDGLMRLLTDAKESIWLTTPYLVPPPELLAILEVAGRSGIDVRIVVPGKGDEWFSFHA
TEYYFEQLLKRNIKIYKYNRHFIHAKTLLIDGKVALVGSANFDFRSMFLNHEMMIAQFDTTSIQELHEAFLKDFDESILI
DWTMLRKKTTGKRFVEAFCHIFSPLL

Sequences:

>Translated_506_residues
MKRRVIQLIIMVISVILLIGLLFWLDVLIPVLIGIAIVAPLVVGWQILFDQVQPQGKLAWLLTVFFIPFIGVLAWVMFGR
TPRRHRRFKRTSSEIRMLRQAIESEKIETSPDVVSSFPLTRTLEKLGATGADAHTSTKLLVNGEEKFDALLDAINSATDH
IHIQYYLFRTDDISLKIRDALIQKSNENVIVRFLYDGLGSQEIGEEFIQPLRESRVHVQAFDPVLHPYLVFNANFRNHQK
LIVVDGRIALTGGLNVGDEYLGKNDKLGFWRDTHLRLEGPLVRELQQLFVENWLYAGQGTSDETWDLFADEKHLSQYFVN
EPKGQNGATQLLVTSPGKDVTIRDGLMRLLTDAKESIWLTTPYLVPPPELLAILEVAGRSGIDVRIVVPGKGDEWFSFHA
TEYYFEQLLKRNIKIYKYNRHFIHAKTLLIDGKVALVGSANFDFRSMFLNHEMMIAQFDTTSIQELHEAFLKDFDESILI
DWTMLRKKTTGKRFVEAFCHIFSPLL
>Mature_506_residues
MKRRVIQLIIMVISVILLIGLLFWLDVLIPVLIGIAIVAPLVVGWQILFDQVQPQGKLAWLLTVFFIPFIGVLAWVMFGR
TPRRHRRFKRTSSEIRMLRQAIESEKIETSPDVVSSFPLTRTLEKLGATGADAHTSTKLLVNGEEKFDALLDAINSATDH
IHIQYYLFRTDDISLKIRDALIQKSNENVIVRFLYDGLGSQEIGEEFIQPLRESRVHVQAFDPVLHPYLVFNANFRNHQK
LIVVDGRIALTGGLNVGDEYLGKNDKLGFWRDTHLRLEGPLVRELQQLFVENWLYAGQGTSDETWDLFADEKHLSQYFVN
EPKGQNGATQLLVTSPGKDVTIRDGLMRLLTDAKESIWLTTPYLVPPPELLAILEVAGRSGIDVRIVVPGKGDEWFSFHA
TEYYFEQLLKRNIKIYKYNRHFIHAKTLLIDGKVALVGSANFDFRSMFLNHEMMIAQFDTTSIQELHEAFLKDFDESILI
DWTMLRKKTTGKRFVEAFCHIFSPLL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=470, Percent_Identity=24.6808510638298, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1787007, Length=363, Percent_Identity=27.8236914600551, Blast_Score=131, Evalue=9e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 57995; Mature: 57995

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRRVIQLIIMVISVILLIGLLFWLDVLIPVLIGIAIVAPLVVGWQILFDQVQPQGKLAW
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
LLTVFFIPFIGVLAWVMFGRTPRRHRRFKRTSSEIRMLRQAIESEKIETSPDVVSSFPLT
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHH
RTLEKLGATGADAHTSTKLLVNGEEKFDALLDAINSATDHIHIQYYLFRTDDISLKIRDA
HHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEEEEECCCCEEEEHHH
LIQKSNENVIVRFLYDGLGSQEIGEEFIQPLRESRVHVQAFDPVLHPYLVFNANFRNHQK
HHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEECCHHHCCEEEEECCCCCCCE
LIVVDGRIALTGGLNVGDEYLGKNDKLGFWRDTHLRLEGPLVRELQQLFVENWLYAGQGT
EEEECCEEEEECCCCCCHHHCCCCCCCCEEECCCEEECCHHHHHHHHHHHHHCEECCCCC
SDETWDLFADEKHLSQYFVNEPKGQNGATQLLVTSPGKDVTIRDGLMRLLTDAKESIWLT
CCCCCCHHCCHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEHHHHHHHHHHHCCCCEEEE
TPYLVPPPELLAILEVAGRSGIDVRIVVPGKGDEWFSFHATEYYFEQLLKRNIKIYKYNR
CCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCEEECHHHHHHHHHHHCCCEEEEECC
HFIHAKTLLIDGKVALVGSANFDFRSMFLNHEMMIAQFDTTSIQELHEAFLKDFDESILI
EEEEEEEEEEECEEEEEECCCCCHHHHHHCCEEEEEECCCHHHHHHHHHHHHCCCCCEEE
DWTMLRKKTTGKRFVEAFCHIFSPLL
EHHHHHHHCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKRRVIQLIIMVISVILLIGLLFWLDVLIPVLIGIAIVAPLVVGWQILFDQVQPQGKLAW
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
LLTVFFIPFIGVLAWVMFGRTPRRHRRFKRTSSEIRMLRQAIESEKIETSPDVVSSFPLT
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHH
RTLEKLGATGADAHTSTKLLVNGEEKFDALLDAINSATDHIHIQYYLFRTDDISLKIRDA
HHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEEEEECCCCEEEEHHH
LIQKSNENVIVRFLYDGLGSQEIGEEFIQPLRESRVHVQAFDPVLHPYLVFNANFRNHQK
HHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEECCHHHCCEEEEECCCCCCCE
LIVVDGRIALTGGLNVGDEYLGKNDKLGFWRDTHLRLEGPLVRELQQLFVENWLYAGQGT
EEEECCEEEEECCCCCCHHHCCCCCCCCEEECCCEEECCHHHHHHHHHHHHHCEECCCCC
SDETWDLFADEKHLSQYFVNEPKGQNGATQLLVTSPGKDVTIRDGLMRLLTDAKESIWLT
CCCCCCHHCCHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEHHHHHHHHHHHCCCCEEEE
TPYLVPPPELLAILEVAGRSGIDVRIVVPGKGDEWFSFHATEYYFEQLLKRNIKIYKYNR
CCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCEEECHHHHHHHHHHHCCCEEEEECC
HFIHAKTLLIDGKVALVGSANFDFRSMFLNHEMMIAQFDTTSIQELHEAFLKDFDESILI
EEEEEEEEEEECEEEEEECCCCCHHHHHHCCEEEEEECCCHHHHHHHHHHHHCCCCCEEE
DWTMLRKKTTGKRFVEAFCHIFSPLL
EHHHHHHHCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]