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The map label for this gene is speE1 [H]

Identifier: 229917109

GI number: 229917109

Start: 1354139

End: 1354978

Strand: Reverse

Name: speE1 [H]

Synonym: EAT1b_1383

Alternate gene names: 229917109

Gene position: 1354978-1354139 (Counterclockwise)

Preceding gene: 229917114

Following gene: 229917108

Centisome position: 45.17

GC content: 46.9

Gene sequence:

>840_bases
ATGGACCAAAAATTAAAATTATGGTTTACGGAGCACCAAACAGAAGATTACGGGATTACGTTCCGTGTAAACCACGTTTA
TGAGAGCGAACAAACGGAGTTTCAACGACTAGAGATGGTCGAAACGGACGAGTTCGGAACGATGTTGTTACTTGACGGGA
TGGTCATGACGACGGATAAGGACGAATTCGTCTATCACGAGATGGTCGCACACGTCCCACTCTTTACACACCCGAATCCG
AAGCATGTGCTCGTAGTCGGTGGGGGAGACGGCGGTGTCATCCGTGAAATTATGAAACACCCATCAGTAGAAAAAGCGGT
TCTCGTAGATATCGACGGGAAAGTTATTGAATATTCGAAGAAATACTTACCAAATATTGCGGGTGAGCTTGATAACCCGC
GTGTTGAAGTCATCGTTGGTGACGGATTCATGCATATCGCGAAGTCAGAAAACGAGTACGACGTCATTATGGTCGACTCG
ACTGAACCGGTCGGACCAGCAGCCAACCTCTTCACAAAAGGGTTCTATGCAGGGATTTCAAAAGCGCTCAAAGAGGATGG
AATCTTTGTGGCTCAAACGGATAACCCATGGTTCACACCAGAACTCATCCAAACGGTTCAACGTGACGTGAAGGAAATCT
TCCCAATCACGAAATTGTACACAGCAAACGTACCGACGTACCCGAGCGGCATGTGGACGTTCACAATCGGTTCGAAGAAA
TACGATCCGGAACAAGTATCAGCGGAGCGTTTCCATGACATCGAAACGAAGTACTATACGCCGAAGCTTCACAATGCAGC
GTTCGTTCTTCCGAAATTCGTGGAGGACTTAACAAAATGA

Upstream 100 bases:

>100_bases
ATGGGTACAGGTTTTGTATACTTCCTATCGTATGATTAAGAAGCGCCTCAAAACGCCACTCGCATACGAACAACTTTTTG
AACAGAAAGGGGTTCTATCG

Downstream 100 bases:

>100_bases
TTAAGCGTTTCGATGAAGCCTACTCAGGTAAAGTGTTTATCGCCAGCCTCCCAGAGGTGACAGAGGCGAAGACGGTTCTT
TACGGGATGCCGATGGACTG

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase 1; PAPT 1; SPDSY 1 [H]

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MDQKLKLWFTEHQTEDYGITFRVNHVYESEQTEFQRLEMVETDEFGTMLLLDGMVMTTDKDEFVYHEMVAHVPLFTHPNP
KHVLVVGGGDGGVIREIMKHPSVEKAVLVDIDGKVIEYSKKYLPNIAGELDNPRVEVIVGDGFMHIAKSENEYDVIMVDS
TEPVGPAANLFTKGFYAGISKALKEDGIFVAQTDNPWFTPELIQTVQRDVKEIFPITKLYTANVPTYPSGMWTFTIGSKK
YDPEQVSAERFHDIETKYYTPKLHNAAFVLPKFVEDLTK

Sequences:

>Translated_279_residues
MDQKLKLWFTEHQTEDYGITFRVNHVYESEQTEFQRLEMVETDEFGTMLLLDGMVMTTDKDEFVYHEMVAHVPLFTHPNP
KHVLVVGGGDGGVIREIMKHPSVEKAVLVDIDGKVIEYSKKYLPNIAGELDNPRVEVIVGDGFMHIAKSENEYDVIMVDS
TEPVGPAANLFTKGFYAGISKALKEDGIFVAQTDNPWFTPELIQTVQRDVKEIFPITKLYTANVPTYPSGMWTFTIGSKK
YDPEQVSAERFHDIETKYYTPKLHNAAFVLPKFVEDLTK
>Mature_279_residues
MDQKLKLWFTEHQTEDYGITFRVNHVYESEQTEFQRLEMVETDEFGTMLLLDGMVMTTDKDEFVYHEMVAHVPLFTHPNP
KHVLVVGGGDGGVIREIMKHPSVEKAVLVDIDGKVIEYSKKYLPNIAGELDNPRVEVIVGDGFMHIAKSENEYDVIMVDS
TEPVGPAANLFTKGFYAGISKALKEDGIFVAQTDNPWFTPELIQTVQRDVKEIFPITKLYTANVPTYPSGMWTFTIGSKK
YDPEQVSAERFHDIETKYYTPKLHNAAFVLPKFVEDLTK

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=276, Percent_Identity=37.3188405797101, Blast_Score=225, Evalue=3e-59,
Organism=Escherichia coli, GI1786312, Length=280, Percent_Identity=38.5714285714286, Blast_Score=218, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI25154106, Length=265, Percent_Identity=39.2452830188679, Blast_Score=203, Evalue=6e-53,
Organism=Caenorhabditis elegans, GI212645616, Length=222, Percent_Identity=42.3423423423423, Blast_Score=197, Evalue=4e-51,
Organism=Saccharomyces cerevisiae, GI6325326, Length=276, Percent_Identity=38.4057971014493, Blast_Score=218, Evalue=7e-58,
Organism=Saccharomyces cerevisiae, GI6323175, Length=282, Percent_Identity=35.4609929078014, Blast_Score=193, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24645443, Length=282, Percent_Identity=39.3617021276596, Blast_Score=235, Evalue=3e-62,
Organism=Drosophila melanogaster, GI21355941, Length=212, Percent_Identity=40.5660377358491, Blast_Score=184, Evalue=8e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 31805; Mature: 31805

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1 ; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQKLKLWFTEHQTEDYGITFRVNHVYESEQTEFQRLEMVETDEFGTMLLLDGMVMTTDK
CCCCEEEEEEECCCCCCEEEEEEEEEECCCHHHHHHHHEEECCCCCEEEEECCEEEECCC
DEFVYHEMVAHVPLFTHPNPKHVLVVGGGDGGVIREIMKHPSVEKAVLVDIDGKVIEYSK
HHHHHHHHHHHCCEEECCCCCEEEEEECCCCHHHHHHHHCCCCCEEEEEECCCCCEEHHH
KYLPNIAGELDNPRVEVIVGDGFMHIAKSENEYDVIMVDSTEPVGPAANLFTKGFYAGIS
HHCCHHHCCCCCCEEEEEECCCEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHH
KALKEDGIFVAQTDNPWFTPELIQTVQRDVKEIFPITKLYTANVPTYPSGMWTFTIGSKK
HHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCHHEEECCCCCCCCCEEEEEECCCC
YDPEQVSAERFHDIETKYYTPKLHNAAFVLPKFVEDLTK
CCHHHHHHHHHHCCCCEEECCCCCCCEEHHHHHHHHHCC
>Mature Secondary Structure
MDQKLKLWFTEHQTEDYGITFRVNHVYESEQTEFQRLEMVETDEFGTMLLLDGMVMTTDK
CCCCEEEEEEECCCCCCEEEEEEEEEECCCHHHHHHHHEEECCCCCEEEEECCEEEECCC
DEFVYHEMVAHVPLFTHPNPKHVLVVGGGDGGVIREIMKHPSVEKAVLVDIDGKVIEYSK
HHHHHHHHHHHCCEEECCCCCEEEEEECCCCHHHHHHHHCCCCCEEEEEECCCCCEEHHH
KYLPNIAGELDNPRVEVIVGDGFMHIAKSENEYDVIMVDSTEPVGPAANLFTKGFYAGIS
HHCCHHHCCCCCCEEEEEECCCEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHH
KALKEDGIFVAQTDNPWFTPELIQTVQRDVKEIFPITKLYTANVPTYPSGMWTFTIGSKK
HHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCHHEEECCCCCCCCCEEEEEECCCC
YDPEQVSAERFHDIETKYYTPKLHNAAFVLPKFVEDLTK
CCHHHHHHHHHHCCCCEEECCCCCCCEEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]