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The map label for this gene is mscL

Identifier: 229917020

GI number: 229917020

Start: 1269965

End: 1270345

Strand: Direct

Name: mscL

Synonym: EAT1b_1294

Alternate gene names: 229917020

Gene position: 1269965-1270345 (Clockwise)

Preceding gene: 229917009

Following gene: 229917025

Centisome position: 42.33

GC content: 43.83

Gene sequence:

>381_bases
ATGTGGAAAGAATTTAAAAAATTCGCGATGCGTGGGAATGTCATCGACTTAGCCGTCGCCGTCGTACTCGGCGCGGCCTT
CACGGCTATCGTGAATTCACTCGTCAACGATATCTTTATGCCGCTTCTCGGAATCATTATCGGAGGAATCGATTTTTCAA
GCTTAAAAGCGAGTATCTTAGGCGTTGACGTTTTATACGGCAATTTCATACAACAGATTGTATCCTTCTTCTTGATTGCC
ATCGCCCTCTTCTTGATTGTGAAAGTCATCAATCGTCTACAACGCGAGAAAGAAGTCGAAGAAGCTGCTATTCCGACCCC
AACGAAAGAAGAGCAACTGTTGACAGAGATTCGTGATCTGCTAAAAGATCGTTCTTTATGA

Upstream 100 bases:

>100_bases
AAAGTCGAACAAGCATCCTTCCCTGATAGAAGTCGAACGATTTATTTCTTTTCCTTCAAATATGCTACGCTTAAACTATT
CAACGTCAGGAGGTTTGTTT

Downstream 100 bases:

>100_bases
ATGCGAAGGCGCCCGACTACTGTCGTGGCGCCTTTTCAATGAGGTGACGCACGCGTTGGACCGCATTCGTCAGCAACCGA
GCTGCTTCCCAAATCGCTTC

Product: large conductance mechanosensitive channel protein

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 126; Mature: 126

Protein sequence:

>126_residues
MWKEFKKFAMRGNVIDLAVAVVLGAAFTAIVNSLVNDIFMPLLGIIIGGIDFSSLKASILGVDVLYGNFIQQIVSFFLIA
IALFLIVKVINRLQREKEVEEAAIPTPTKEEQLLTEIRDLLKDRSL

Sequences:

>Translated_126_residues
MWKEFKKFAMRGNVIDLAVAVVLGAAFTAIVNSLVNDIFMPLLGIIIGGIDFSSLKASILGVDVLYGNFIQQIVSFFLIA
IALFLIVKVINRLQREKEVEEAAIPTPTKEEQLLTEIRDLLKDRSL
>Mature_126_residues
MWKEFKKFAMRGNVIDLAVAVVLGAAFTAIVNSLVNDIFMPLLGIIIGGIDFSSLKASILGVDVLYGNFIQQIVSFFLIA
IALFLIVKVINRLQREKEVEEAAIPTPTKEEQLLTEIRDLLKDRSL

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=130, Percent_Identity=56.1538461538462, Blast_Score=139, Evalue=5e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_EXISA (C4KYQ8)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002885666.1
- GeneID:   7868721
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_1294
- OMA:   GANVLAW
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 14022; Mature: 14022

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x2672f418)-; HASH(0x40ea3180)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWKEFKKFAMRGNVIDLAVAVVLGAAFTAIVNSLVNDIFMPLLGIIIGGIDFSSLKASIL
CCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
GVDVLYGNFIQQIVSFFLIAIALFLIVKVINRLQREKEVEEAAIPTPTKEEQLLTEIRDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
LKDRSL
HHHCCH
>Mature Secondary Structure
MWKEFKKFAMRGNVIDLAVAVVLGAAFTAIVNSLVNDIFMPLLGIIIGGIDFSSLKASIL
CCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
GVDVLYGNFIQQIVSFFLIAIALFLIVKVINRLQREKEVEEAAIPTPTKEEQLLTEIRDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
LKDRSL
HHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA