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The map label for this gene is carB [C]

Identifier: 229916916

GI number: 229916916

Start: 1159916

End: 1161142

Strand: Reverse

Name: carB [C]

Synonym: EAT1b_1189

Alternate gene names: 229916916

Gene position: 1161142-1159916 (Counterclockwise)

Preceding gene: 229916917

Following gene: 229916915

Centisome position: 38.71

GC content: 52.16

Gene sequence:

>1227_bases
ATGCATCAAGTTCCCATCATCCAAAAAGCGAAACAACAAGGGATATTCACCGTTCTCTGTGACTATTTACCGGATAATCC
AGGTCGACTCGTGGCCGACCGTTATTATTGTACGAGCACCCTTGATCGGGAGGCGGTCCTCGCCATTGCGAGAGAGGAAG
GCATCGATGGGATTGTCGCCTATGCCTCCGATCCGGCGGCGCGAACGGCCGCCTATGTGGGAGAAACACTCGGGTTAGTG
ACGAACTCGCTTGAAACGGTCGAAACGTTGACGAATAAAGCGAAGTTTCGGACATTCCTACAGCGACATGACTTTGCCGT
CCCGAAAACCTTTGCGTTCACCGAACTGGAAACGGCGGTCGACACGTTATGGGAGCGGGCAGGTGAATATATCATCAAAC
CGGCCGACGCCTCAGGGAGTAAAGGAATCAGCAAGTGGAGTCGGGGGCACGGGATTGAGCAGCTTCGTCGCCATCTTCAA
GTGGCATTTACGATGTCATTCAATCAGGAAGTAGTCGTGGAGGAGTTCATTACCCGAGACGGCTATCAATTAGCGGGTGA
TGGATTTGTCGTCGACGGAGAGTTGGTGTTTCGTTGTTTCGGAAACGGACACTTCAACGTGAACGGAGTCAACCCGTTCG
CCCCGATCAGTGCGAGTTTTCCGAGTGTGCATCCGTCTCACGTGCTTGAGCAAGTCCATGAGACGTTGCAACGCATGTTG
ACTGAAGTCGGCTTCAAGCAAGGGGCGTTCAACTTCGATATCTTCGTCAAAGGAGAAGACGTCTATCTGATGGATATCGG
TCCACGGAACGGAGGGAATTACATCCCACAAATCATCGAACAAGCGACAGGCGTCGACTTGGTCCAGGCGACGATTGATG
TCGCACTCGGGATGCCGGTGACGATCGAGATGAAACCAGTGACCGTACCGTCTGCCTATTTCATCATCGGCAGTCAAGAA
ACGGGGCGATTCATGGGCGTCCATTTCGATGAAGATGCATATACGATTCGACAAGCGAACATCTATTATAACGTCGGAGA
TGTGGTCCCGCGCTTCGAAGGGGCAAATCAAGCACTCGGTAGTTTGATTCTAGAGTTTGAAGACGTCGAGACGATGCTTC
GACTCGTCGAACATCCCGAGCGATGGCTGACCTTACACATCAAGTCGACCGAAGAAACGGAGGGAGAATCATGGACATCG
TCGTCACCGGTGCGAACGGTTTTTTAG

Upstream 100 bases:

>100_bases
CTGAACGCCAACACGGCTGCCGTCTACGTCGATTTGCTCCAACCACTCTACCGCAAAGGAGATTCTCACCATGCTGAAGC
TGCTGTTGCTCGGGGGAGCG

Downstream 100 bases:

>100_bases
GCAGACACCTCGTCCACGCCTTGGAGCGGGCTGGGCATCTCGTCCTCCCGATCACCCGTTCGACCTCATCCGTGGAACGA
AGCAGCTATCTGCGTCGAGC

Product: carbamoyl-phosphate-synthetase

Products: ADP; Carbamoyl phosphate; L-Glutamate; H+; Phosphate [C]

Alternate protein names: Carbamoyl-Phosphate Synthase Large Chain; L-Lactate Dehydrogenase; Phosphoribosylglycinamide Synthetase; Biotin Carboxylase; Carbamoyl-Phosphate-Synthetase Protein; CarB Family Protein; Carbamoyl-Phosphate Synthase Large Subunit; Carbamoyl-Phosphate Synthase Small Subunit; Dehydrogenase; Glycolate Oxidase; Carbamoyl Phosphate Synthase-Like Protein; Phosphoribosylglycinamide Synthetase ATP-Grasp; Oxidase Or Carboxylase

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MHQVPIIQKAKQQGIFTVLCDYLPDNPGRLVADRYYCTSTLDREAVLAIAREEGIDGIVAYASDPAARTAAYVGETLGLV
TNSLETVETLTNKAKFRTFLQRHDFAVPKTFAFTELETAVDTLWERAGEYIIKPADASGSKGISKWSRGHGIEQLRRHLQ
VAFTMSFNQEVVVEEFITRDGYQLAGDGFVVDGELVFRCFGNGHFNVNGVNPFAPISASFPSVHPSHVLEQVHETLQRML
TEVGFKQGAFNFDIFVKGEDVYLMDIGPRNGGNYIPQIIEQATGVDLVQATIDVALGMPVTIEMKPVTVPSAYFIIGSQE
TGRFMGVHFDEDAYTIRQANIYYNVGDVVPRFEGANQALGSLILEFEDVETMLRLVEHPERWLTLHIKSTEETEGESWTS
SSPVRTVF

Sequences:

>Translated_408_residues
MHQVPIIQKAKQQGIFTVLCDYLPDNPGRLVADRYYCTSTLDREAVLAIAREEGIDGIVAYASDPAARTAAYVGETLGLV
TNSLETVETLTNKAKFRTFLQRHDFAVPKTFAFTELETAVDTLWERAGEYIIKPADASGSKGISKWSRGHGIEQLRRHLQ
VAFTMSFNQEVVVEEFITRDGYQLAGDGFVVDGELVFRCFGNGHFNVNGVNPFAPISASFPSVHPSHVLEQVHETLQRML
TEVGFKQGAFNFDIFVKGEDVYLMDIGPRNGGNYIPQIIEQATGVDLVQATIDVALGMPVTIEMKPVTVPSAYFIIGSQE
TGRFMGVHFDEDAYTIRQANIYYNVGDVVPRFEGANQALGSLILEFEDVETMLRLVEHPERWLTLHIKSTEETEGESWTS
SSPVRTVF
>Mature_408_residues
MHQVPIIQKAKQQGIFTVLCDYLPDNPGRLVADRYYCTSTLDREAVLAIAREEGIDGIVAYASDPAARTAAYVGETLGLV
TNSLETVETLTNKAKFRTFLQRHDFAVPKTFAFTELETAVDTLWERAGEYIIKPADASGSKGISKWSRGHGIEQLRRHLQ
VAFTMSFNQEVVVEEFITRDGYQLAGDGFVVDGELVFRCFGNGHFNVNGVNPFAPISASFPSVHPSHVLEQVHETLQRML
TEVGFKQGAFNFDIFVKGEDVYLMDIGPRNGGNYIPQIIEQATGVDLVQATIDVALGMPVTIEMKPVTVPSAYFIIGSQE
TGRFMGVHFDEDAYTIRQANIYYNVGDVVPRFEGANQALGSLILEFEDVETMLRLVEHPERWLTLHIKSTEETEGESWTS
SSPVRTVF

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0439

COG function: function code I; Biotin carboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786216, Length=336, Percent_Identity=24.702380952381, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 4701 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.5.5 [C]

Molecular weight: Translated: 45417; Mature: 45417

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHQVPIIQKAKQQGIFTVLCDYLPDNPGRLVADRYYCTSTLDREAVLAIAREEGIDGIVA
CCCCCHHHHHHHCCCEEEEEHHCCCCCCCEEEEEEEECCCCCHHHHHEEHHHCCCCEEEE
YASDPAARTAAYVGETLGLVTNSLETVETLTNKAKFRTFLQRHDFAVPKTFAFTELETAV
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHH
DTLWERAGEYIIKPADASGSKGISKWSRGHGIEQLRRHLQVAFTMSFNQEVVVEEFITRD
HHHHHHCCCEEEECCCCCCCCCHHHHHCCCCHHHHHHHHEEEEEECCCCHHHHHHHHCCC
GYQLAGDGFVVDGELVFRCFGNGHFNVNGVNPFAPISASFPSVHPSHVLEQVHETLQRML
CCEECCCCEEECCEEEEEEECCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
TEVGFKQGAFNFDIFVKGEDVYLMDIGPRNGGNYIPQIIEQATGVDLVQATIDVALGMPV
HHCCCCCCCEEEEEEEECCEEEEEECCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCE
TIEMKPVTVPSAYFIIGSQETGRFMGVHFDEDAYTIRQANIYYNVGDVVPRFEGANQALG
EEEEECEECCCEEEEEECCCCCCEEEEEECCCCEEEEEEEEEEECCCCCCCCCCHHHHHH
SLILEFEDVETMLRLVEHPERWLTLHIKSTEETEGESWTSSSPVRTVF
HHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCEECC
>Mature Secondary Structure
MHQVPIIQKAKQQGIFTVLCDYLPDNPGRLVADRYYCTSTLDREAVLAIAREEGIDGIVA
CCCCCHHHHHHHCCCEEEEEHHCCCCCCCEEEEEEEECCCCCHHHHHEEHHHCCCCEEEE
YASDPAARTAAYVGETLGLVTNSLETVETLTNKAKFRTFLQRHDFAVPKTFAFTELETAV
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHH
DTLWERAGEYIIKPADASGSKGISKWSRGHGIEQLRRHLQVAFTMSFNQEVVVEEFITRD
HHHHHHCCCEEEECCCCCCCCCHHHHHCCCCHHHHHHHHEEEEEECCCCHHHHHHHHCCC
GYQLAGDGFVVDGELVFRCFGNGHFNVNGVNPFAPISASFPSVHPSHVLEQVHETLQRML
CCEECCCCEEECCEEEEEEECCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
TEVGFKQGAFNFDIFVKGEDVYLMDIGPRNGGNYIPQIIEQATGVDLVQATIDVALGMPV
HHCCCCCCCEEEEEEEECCEEEEEECCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCE
TIEMKPVTVPSAYFIIGSQETGRFMGVHFDEDAYTIRQANIYYNVGDVVPRFEGANQALG
EEEEECEECCCEEEEEECCCCCCEEEEEECCCCEEEEEEEEEEECCCCCCCCCCHHHHHH
SLILEFEDVETMLRLVEHPERWLTLHIKSTEETEGESWTSSSPVRTVF
HHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: AMP; GDP; GMP; GTP; IDP; IMP; ITP; N-Acetylglutamate; NEM; Orn; Thiol; XMP [C]

Metal ions: K+; Mg2+; Mn2+; NH4+ [C]

Kcat value (1/min): 120000 [C]

Specific activity: NA

Km value (mM): 93 {NH4+}} 0.38 {L-Gln}} [C]

Substrates: ATP; L-Glutamine; H2O; Bicarbonate [C]

Specific reaction: (2) ATP + L-Glutamine + H2O + Bicarbonate --> (2) ADP + Carbamoyl phosphate + L-Glutamate + (2) H+ + Phosphate [C]

General reaction: Amide group transfer; Amination; Phosphorylation [C]

Inhibitor: 6-Diazo-5-oxon/leucine; Alkyl hydrazines; Azaserine; Guanidine; H2O2; NH2OH; L-2-Amino-4-oxo-5-chloropentanoate; L-Cyanate; NEM; P1, P5-Di (adenosine 5')-pentaphosphate; UDP; UMP phosphate; Urea; UTP [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA