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The map label for this gene is ebrB [H]

Identifier: 229916854

GI number: 229916854

Start: 1102456

End: 1102764

Strand: Direct

Name: ebrB [H]

Synonym: EAT1b_1127

Alternate gene names: 229916854

Gene position: 1102456-1102764 (Clockwise)

Preceding gene: 229916853

Following gene: 229916855

Centisome position: 36.75

GC content: 54.05

Gene sequence:

>309_bases
ATGGGATTCATCTATTTGATCGGGGCCATCGCCTCGGAACTATTCGGGTCGAGCATGTTGAAAGTGAATGCCACCTCGAC
GAGCCGTCTCCCGATTCTCGGGGTCGCCTTCGGTTATGTGACGGCGTTTTATCTCTTATCACTTGCCCTCTTCACCATCC
CGCTCAGCTTCGCGTATGCGTTTTGGAGCGGGGTTGGGACGGCACTCACGGCAGTGATCGGTTTCGTCGTCTTCCGGGAA
CCAATCAGTCGTCAAAAAATTGCAGGGATTGGGCTCGTCATCATCGGGCTCGTCTTGATGAAAGTGTGA

Upstream 100 bases:

>100_bases
TGGTTCGCTGTCTTGTATGGATATAGCGAATCGTTCAAGGACCGTGCGGCGACACTCATTCGGGAGGCATGTGATGAGTT
GCTTCAAGGAGAATTGTAAT

Downstream 100 bases:

>100_bases
GGTGAATCGGAATGAAAGCCTATCTTTTACTCTTCTTTTCTATCGGATTTGAGGTACTCGGGACGTCGATGTTGAAACTG
TCGGATGGATTCACGAATCT

Product: small multidrug resistance protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 102; Mature: 101

Protein sequence:

>102_residues
MGFIYLIGAIASELFGSSMLKVNATSTSRLPILGVAFGYVTAFYLLSLALFTIPLSFAYAFWSGVGTALTAVIGFVVFRE
PISRQKIAGIGLVIIGLVLMKV

Sequences:

>Translated_102_residues
MGFIYLIGAIASELFGSSMLKVNATSTSRLPILGVAFGYVTAFYLLSLALFTIPLSFAYAFWSGVGTALTAVIGFVVFRE
PISRQKIAGIGLVIIGLVLMKV
>Mature_101_residues
GFIYLIGAIASELFGSSMLKVNATSTSRLPILGVAFGYVTAFYLLSLALFTIPLSFAYAFWSGVGTALTAVIGFVVFREP
ISRQKIAGIGLVIIGLVLMKV

Specific function: Part of a multidrug efflux pump. Confers resistance to cationic lipophilic dyes such as ethidium bromide, acriflavine, pyronine Y and safranin O. The efflux is probably coupled to an influx of protons [H]

COG id: COG2076

COG function: function code P; Membrane transporters of cations and cationic drugs

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the small multidrug resistance (SMR) protein family. EbrA/ebrB (TC 2.A.7.1.6) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786755, Length=100, Percent_Identity=33, Blast_Score=59, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000390 [H]

Pfam domain/function: PF00893 Multi_Drug_Res [H]

EC number: NA

Molecular weight: Translated: 10840; Mature: 10709

Theoretical pI: Translated: 10.25; Mature: 10.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFIYLIGAIASELFGSSMLKVNATSTSRLPILGVAFGYVTAFYLLSLALFTIPLSFAYA
CCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FWSGVGTALTAVIGFVVFREPISRQKIAGIGLVIIGLVLMKV
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GFIYLIGAIASELFGSSMLKVNATSTSRLPILGVAFGYVTAFYLLSLALFTIPLSFAYA
CHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FWSGVGTALTAVIGFVVFREPISRQKIAGIGLVIIGLVLMKV
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10735876; 9384377 [H]