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The map label for this gene is rpfG [H]

Identifier: 229916769

GI number: 229916769

Start: 989364

End: 992048

Strand: Reverse

Name: rpfG [H]

Synonym: EAT1b_1042

Alternate gene names: 229916769

Gene position: 992048-989364 (Counterclockwise)

Preceding gene: 229916772

Following gene: 229916765

Centisome position: 33.07

GC content: 49.76

Gene sequence:

>2685_bases
GTGACAAACCCCCATCATTCTTCATACGACACATTGTTGCGTTATATGCCCCAAGGCTACGTCGAGTTTAAAGTGATTTA
CGACGACGCTTCGGAGCCGATTGACTATGAATTCTTTGAAGTGAATCCTGCCTTTGAGAAGATCATGAAAGGGAAGCGAA
AACACTTCATCGGGAAAAAAGTATCAAGCGCCTTAAAGGACTTTGAATATAGTGAGCGGGAGTGGATGATTCAAGTGGCA
CACGTCGTCAACGAAGGCGGAACGGTGACGTTTGAACATTTCTCTCGGCGCAACAAGTCATGGTTCGAGATGTCCGTGTT
CAGTGAACGCCAAGGATTCGTATCGGTCGTGTTCCATGACATCACCCGACATGTTCAAGAAAACAATGCGTTGAAGCAGC
TCGTCAACGTCTCCCATCGCTTCGTCTCGACGGGTAGCGATTTATTGATGAATCAACAATTCCTCGACCAGATTCGTGAC
TTCACGGGAGCGAAGGCGGTCGCGCTCAATTTAATGGAGAACGATGGAGAGTCCTACACGACGGTCGCCTTATCCGGCAT
GGACGAGATGTTAGAAAGAAGTCTCGATATCCTTGGATTTCATCCGGTCGGAAGGAAATGGCCGATGACGGATCAGCGGC
GTGACCTGTTCAAACAATCGAATCCTCGTGTCTTTGATGGGATTGACCCACTGATTGATGGCGCGCTGCCGAAACGGCTC
GTCAAAAATCTACTCCGTATCTTCTCGCTTGGAGAAGTCGTTCTCTTTAAAATCGAAAAAGATGGCGTCTTGTATGGAGA
TTTGACGATTGTGATGGAGCAAGGGAAACAAGCGAATCGCCTCGGTCTCGTCGAGTTGTACCTCGTCCATGTGCTTCAGC
AATTGACGAAAGCCGATCATCAAGTGGGTGAGACGAAGAAATCGATGTTTGATGGATTTGGAACGCTCGACTTGGAATGC
ATCATGGCCTTCAACACACGGCAAGTGCTGCACTTGAACGAAACATGGGAGCGCGTTTTAGGGTTTTCGCTTGATGAGTT
GGAGAATATCGATTTTCTTAGTCTGATTCACCCGGACGATGACGTGATCACGGAAGCAGGGTTTGCGGAACTTGAAGAAC
GTGGCGAGTTGTTCCGATTCGTCAACCGCTTCCGCACAATCGACGATACGTACCGCTCAATCGAATGGCATTGCAAACTT
CAAGGGGATTACATTTATGCGGTCGCCAAGGACGTGACGGATCGGGTCCAATTGGAAGAAAAGTTGGCCGACCGGGACCG
CCTCTTGACGAAACTATCCGACCAAATCCCGGGTGCCATCTATCAATTCGAATTGTTGCCTGACGGAAGCTTCAACTTCC
CGTTCTTTAGCCGGGGCTTTGAAGATATGTTAGGGATTTCGGCAGAAGAGGTACAGGCGGACGCGAGTCTTGTCTTCCGC
CGGATGCACCCGGATGATTATCCCCATGTCATGCAGTCGATTGATGAATCGTATCGTAATTTGACGATTTGGGACGCAGA
GTTTCGTGTGTTGACCGAGGACGGACGAACCATCTGGATTCTCGGGTCATCTCGTCCGGAGAAGATTGCGGAAGGCCATG
TCTTGTGGCACGGCTATATCGGAGATGTCACGGAGAAGAAGACATACGAGAAAGAGATTGAATATCTCAGTTATCACGAT
CAATTGACGGGTATCAAAAACCGACGCTACTTTGAAGAGGCGTTGGAGCGCTATGATGATGAGCATTTTTATCCGCTCAC
ACTCATCGTCATCGATATCAATGGTCTGAAACTCACGAACGATGCGTTCGGTCACTTGGTCGGGGATCAGCTGCTCGTCG
AGGCGGCAAATATTTTACGAAATGAATTGCGCGCGAAAGATGAAGTGGCGCGAGTCGGAGGCGATGAGTTCGTCGTGATC
TCTCCGAATACGACGCTCGATGAGGCGAAGCAACTGTCGGACCGTCTCGGTCGTCTCTTCCATGAACAGACGGTGGAAGG
GTTACCGATTTCCGCATCGTTCGGTGAAGCGGTCAAACATGACGCCACCGTCGCGTTGAGCGAGGTGTTCAACTTAGCAG
AAGACCGGATGTATCACCATAAGGTTTCTGAAAAGCAGAGTCGACGCCACCATTCCATCCAAATCATCATGCGCACCCTG
TATGAGAAGATTCCACGCGAAGAAGCGCACTCTCAACGGGTCGCCAATTTAGCGGGGAAACTTGCGGAAGCGATGGGCTT
CACGAGTGCCGAAGTGAATGAGATTCGAACGGCCGCCATTTTGCACGATATCGGAAAAGTGGCAATCAGTAATGACATTT
TAGATAAACCTGGTGCGTTGACGGAATCGGAATGGTATGAAATCAAGCGCCATCCGGAAATCAGTTACAATATTTTGAGT
ACGGTACCCGAATATGTCGCCTTGTCCGAGATTGTCTTGGCGCACCATGAGCGATGGGACGGTAAAGGATATCCGAAAGG
GTTGAGCGATTATGACATCCCGCTCGCCTCGCGAATTATCGCCGTCGCCGACACGTTCGATGTCATGGTGACGGGGCGAC
CATATCGACAAGCGAAGACGATTGACGAGGCACTTGCCATCATCAAACAAGAGGCAGGGAAACAGTTCGACCCATCTCTC
GTCGACGTCTTCTTTGAAAAAGTCGTACCGACACTTCAACAATAA

Upstream 100 bases:

>100_bases
ACTTAAAATAAAAAGTTGGGAATGAGTTCGGAAAAAATAGGGAATCTATCACAAATGAGGTAAACTAAGTGGAGATTTTA
TTTGCTGAGGAGGAAGTGAC

Downstream 100 bases:

>100_bases
ACAAGCGAGTCCTCCTGAATGTGGGGGACTCGCTTTTTCAAACGGTCAGAGGTCGTTTCTTCAACACGAGGATGGTGAAG
AGGAAGACGATGCCGGTCAT

Product: diguanylate cyclase and metal dependent phosphohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 894; Mature: 893

Protein sequence:

>894_residues
MTNPHHSSYDTLLRYMPQGYVEFKVIYDDASEPIDYEFFEVNPAFEKIMKGKRKHFIGKKVSSALKDFEYSEREWMIQVA
HVVNEGGTVTFEHFSRRNKSWFEMSVFSERQGFVSVVFHDITRHVQENNALKQLVNVSHRFVSTGSDLLMNQQFLDQIRD
FTGAKAVALNLMENDGESYTTVALSGMDEMLERSLDILGFHPVGRKWPMTDQRRDLFKQSNPRVFDGIDPLIDGALPKRL
VKNLLRIFSLGEVVLFKIEKDGVLYGDLTIVMEQGKQANRLGLVELYLVHVLQQLTKADHQVGETKKSMFDGFGTLDLEC
IMAFNTRQVLHLNETWERVLGFSLDELENIDFLSLIHPDDDVITEAGFAELEERGELFRFVNRFRTIDDTYRSIEWHCKL
QGDYIYAVAKDVTDRVQLEEKLADRDRLLTKLSDQIPGAIYQFELLPDGSFNFPFFSRGFEDMLGISAEEVQADASLVFR
RMHPDDYPHVMQSIDESYRNLTIWDAEFRVLTEDGRTIWILGSSRPEKIAEGHVLWHGYIGDVTEKKTYEKEIEYLSYHD
QLTGIKNRRYFEEALERYDDEHFYPLTLIVIDINGLKLTNDAFGHLVGDQLLVEAANILRNELRAKDEVARVGGDEFVVI
SPNTTLDEAKQLSDRLGRLFHEQTVEGLPISASFGEAVKHDATVALSEVFNLAEDRMYHHKVSEKQSRRHHSIQIIMRTL
YEKIPREEAHSQRVANLAGKLAEAMGFTSAEVNEIRTAAILHDIGKVAISNDILDKPGALTESEWYEIKRHPEISYNILS
TVPEYVALSEIVLAHHERWDGKGYPKGLSDYDIPLASRIIAVADTFDVMVTGRPYRQAKTIDEALAIIKQEAGKQFDPSL
VDVFFEKVVPTLQQ

Sequences:

>Translated_894_residues
MTNPHHSSYDTLLRYMPQGYVEFKVIYDDASEPIDYEFFEVNPAFEKIMKGKRKHFIGKKVSSALKDFEYSEREWMIQVA
HVVNEGGTVTFEHFSRRNKSWFEMSVFSERQGFVSVVFHDITRHVQENNALKQLVNVSHRFVSTGSDLLMNQQFLDQIRD
FTGAKAVALNLMENDGESYTTVALSGMDEMLERSLDILGFHPVGRKWPMTDQRRDLFKQSNPRVFDGIDPLIDGALPKRL
VKNLLRIFSLGEVVLFKIEKDGVLYGDLTIVMEQGKQANRLGLVELYLVHVLQQLTKADHQVGETKKSMFDGFGTLDLEC
IMAFNTRQVLHLNETWERVLGFSLDELENIDFLSLIHPDDDVITEAGFAELEERGELFRFVNRFRTIDDTYRSIEWHCKL
QGDYIYAVAKDVTDRVQLEEKLADRDRLLTKLSDQIPGAIYQFELLPDGSFNFPFFSRGFEDMLGISAEEVQADASLVFR
RMHPDDYPHVMQSIDESYRNLTIWDAEFRVLTEDGRTIWILGSSRPEKIAEGHVLWHGYIGDVTEKKTYEKEIEYLSYHD
QLTGIKNRRYFEEALERYDDEHFYPLTLIVIDINGLKLTNDAFGHLVGDQLLVEAANILRNELRAKDEVARVGGDEFVVI
SPNTTLDEAKQLSDRLGRLFHEQTVEGLPISASFGEAVKHDATVALSEVFNLAEDRMYHHKVSEKQSRRHHSIQIIMRTL
YEKIPREEAHSQRVANLAGKLAEAMGFTSAEVNEIRTAAILHDIGKVAISNDILDKPGALTESEWYEIKRHPEISYNILS
TVPEYVALSEIVLAHHERWDGKGYPKGLSDYDIPLASRIIAVADTFDVMVTGRPYRQAKTIDEALAIIKQEAGKQFDPSL
VDVFFEKVVPTLQQ
>Mature_893_residues
TNPHHSSYDTLLRYMPQGYVEFKVIYDDASEPIDYEFFEVNPAFEKIMKGKRKHFIGKKVSSALKDFEYSEREWMIQVAH
VVNEGGTVTFEHFSRRNKSWFEMSVFSERQGFVSVVFHDITRHVQENNALKQLVNVSHRFVSTGSDLLMNQQFLDQIRDF
TGAKAVALNLMENDGESYTTVALSGMDEMLERSLDILGFHPVGRKWPMTDQRRDLFKQSNPRVFDGIDPLIDGALPKRLV
KNLLRIFSLGEVVLFKIEKDGVLYGDLTIVMEQGKQANRLGLVELYLVHVLQQLTKADHQVGETKKSMFDGFGTLDLECI
MAFNTRQVLHLNETWERVLGFSLDELENIDFLSLIHPDDDVITEAGFAELEERGELFRFVNRFRTIDDTYRSIEWHCKLQ
GDYIYAVAKDVTDRVQLEEKLADRDRLLTKLSDQIPGAIYQFELLPDGSFNFPFFSRGFEDMLGISAEEVQADASLVFRR
MHPDDYPHVMQSIDESYRNLTIWDAEFRVLTEDGRTIWILGSSRPEKIAEGHVLWHGYIGDVTEKKTYEKEIEYLSYHDQ
LTGIKNRRYFEEALERYDDEHFYPLTLIVIDINGLKLTNDAFGHLVGDQLLVEAANILRNELRAKDEVARVGGDEFVVIS
PNTTLDEAKQLSDRLGRLFHEQTVEGLPISASFGEAVKHDATVALSEVFNLAEDRMYHHKVSEKQSRRHHSIQIIMRTLY
EKIPREEAHSQRVANLAGKLAEAMGFTSAEVNEIRTAAILHDIGKVAISNDILDKPGALTESEWYEIKRHPEISYNILST
VPEYVALSEIVLAHHERWDGKGYPKGLSDYDIPLASRIIAVADTFDVMVTGRPYRQAKTIDEALAIIKQEAGKQFDPSLV
DVFFEKVVPTLQQ

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=192, Percent_Identity=32.8125, Blast_Score=95, Evalue=2e-20,
Organism=Escherichia coli, GI87082007, Length=165, Percent_Identity=32.1212121212121, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI1788381, Length=261, Percent_Identity=27.5862068965517, Blast_Score=80, Evalue=6e-16,
Organism=Escherichia coli, GI1787262, Length=153, Percent_Identity=31.3725490196078, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1786584, Length=117, Percent_Identity=36.7521367521368, Blast_Score=74, Evalue=5e-14,
Organism=Escherichia coli, GI1787056, Length=159, Percent_Identity=33.3333333333333, Blast_Score=69, Evalue=2e-12,
Organism=Escherichia coli, GI1787541, Length=168, Percent_Identity=29.1666666666667, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI145693134, Length=127, Percent_Identity=30.7086614173228, Blast_Score=66, Evalue=9e-12,
Organism=Escherichia coli, GI1788085, Length=149, Percent_Identity=30.8724832214765, Blast_Score=63, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 102632; Mature: 102501

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNPHHSSYDTLLRYMPQGYVEFKVIYDDASEPIDYEFFEVNPAFEKIMKGKRKHFIGKK
CCCCCCCCHHHHHHHCCCCCEEEEEEEECCCCCCCCEEEECCHHHHHHHCCHHHHHHHHH
VSSALKDFEYSEREWMIQVAHVVNEGGTVTFEHFSRRNKSWFEMSVFSERQGFVSVVFHD
HHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEHHHHCCCCCCEEEHHHHCCCCHHHHHHHH
ITRHVQENNALKQLVNVSHRFVSTGSDLLMNQQFLDQIRDFTGAKAVALNLMENDGESYT
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHEEEEEHHCCCCCEE
TVALSGMDEMLERSLDILGFHPVGRKWPMTDQRRDLFKQSNPRVFDGIDPLIDGALPKRL
EEEECCHHHHHHHCCHHEECCCCCCCCCCCHHHHHHHHCCCCCEECCCHHHHCCHHHHHH
VKNLLRIFSLGEVVLFKIEKDGVLYGDLTIVMEQGKQANRLGLVELYLVHVLQQLTKADH
HHHHHHHHCCCCEEEEEEECCCEEEEHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
QVGETKKSMFDGFGTLDLECIMAFNTRQVLHLNETWERVLGFSLDELENIDFLSLIHPDD
HHHHHHHHHHCCCCCCCEEEEEEECCCEEEEECHHHHHHHCCCHHHHCCCCEEEEECCCH
DVITEAGFAELEERGELFRFVNRFRTIDDTYRSIEWHCKLQGDYIYAVAKDVTDRVQLEE
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCEEEEEHHHHHHHHHHHH
KLADRDRLLTKLSDQIPGAIYQFELLPDGSFNFPFFSRGFEDMLGISAEEVQADASLVFR
HHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHCCHHHHHCCCHHHHHHHHHHHHH
RMHPDDYPHVMQSIDESYRNLTIWDAEFRVLTEDGRTIWILGSSRPEKIAEGHVLWHGYI
HCCCCCHHHHHHHHHHHHCCEEEEECEEEEEECCCCEEEEEECCCCCHHHCCCEEEEECC
GDVTEKKTYEKEIEYLSYHDQLTGIKNRRYFEEALERYDDEHFYPLTLIVIDINGLKLTN
CCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEEEEEECCEEECC
DAFGHLVGDQLLVEAANILRNELRAKDEVARVGGDEFVVISPNTTLDEAKQLSDRLGRLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHH
HEQTVEGLPISASFGEAVKHDATVALSEVFNLAEDRMYHHKVSEKQSRRHHSIQIIMRTL
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YEKIPREEAHSQRVANLAGKLAEAMGFTSAEVNEIRTAAILHDIGKVAISNDILDKPGAL
HHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
TESEWYEIKRHPEISYNILSTVPEYVALSEIVLAHHERWDGKGYPKGLSDYDIPLASRII
CCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHH
AVADTFDVMVTGRPYRQAKTIDEALAIIKQEAGKQFDPSLVDVFFEKVVPTLQQ
HHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TNPHHSSYDTLLRYMPQGYVEFKVIYDDASEPIDYEFFEVNPAFEKIMKGKRKHFIGKK
CCCCCCCHHHHHHHCCCCCEEEEEEEECCCCCCCCEEEECCHHHHHHHCCHHHHHHHHH
VSSALKDFEYSEREWMIQVAHVVNEGGTVTFEHFSRRNKSWFEMSVFSERQGFVSVVFHD
HHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEHHHHCCCCCCEEEHHHHCCCCHHHHHHHH
ITRHVQENNALKQLVNVSHRFVSTGSDLLMNQQFLDQIRDFTGAKAVALNLMENDGESYT
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHEEEEEHHCCCCCEE
TVALSGMDEMLERSLDILGFHPVGRKWPMTDQRRDLFKQSNPRVFDGIDPLIDGALPKRL
EEEECCHHHHHHHCCHHEECCCCCCCCCCCHHHHHHHHCCCCCEECCCHHHHCCHHHHHH
VKNLLRIFSLGEVVLFKIEKDGVLYGDLTIVMEQGKQANRLGLVELYLVHVLQQLTKADH
HHHHHHHHCCCCEEEEEEECCCEEEEHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
QVGETKKSMFDGFGTLDLECIMAFNTRQVLHLNETWERVLGFSLDELENIDFLSLIHPDD
HHHHHHHHHHCCCCCCCEEEEEEECCCEEEEECHHHHHHHCCCHHHHCCCCEEEEECCCH
DVITEAGFAELEERGELFRFVNRFRTIDDTYRSIEWHCKLQGDYIYAVAKDVTDRVQLEE
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCEEEEEHHHHHHHHHHHH
KLADRDRLLTKLSDQIPGAIYQFELLPDGSFNFPFFSRGFEDMLGISAEEVQADASLVFR
HHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHCCHHHHHCCCHHHHHHHHHHHHH
RMHPDDYPHVMQSIDESYRNLTIWDAEFRVLTEDGRTIWILGSSRPEKIAEGHVLWHGYI
HCCCCCHHHHHHHHHHHHCCEEEEECEEEEEECCCCEEEEEECCCCCHHHCCCEEEEECC
GDVTEKKTYEKEIEYLSYHDQLTGIKNRRYFEEALERYDDEHFYPLTLIVIDINGLKLTN
CCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEEEEEEECCEEECC
DAFGHLVGDQLLVEAANILRNELRAKDEVARVGGDEFVVISPNTTLDEAKQLSDRLGRLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHH
HEQTVEGLPISASFGEAVKHDATVALSEVFNLAEDRMYHHKVSEKQSRRHHSIQIIMRTL
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YEKIPREEAHSQRVANLAGKLAEAMGFTSAEVNEIRTAAILHDIGKVAISNDILDKPGAL
HHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
TESEWYEIKRHPEISYNILSTVPEYVALSEIVLAHHERWDGKGYPKGLSDYDIPLASRII
CCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHH
AVADTFDVMVTGRPYRQAKTIDEALAIIKQEAGKQFDPSLVDVFFEKVVPTLQQ
HHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]