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The map label for this gene is ydfA [H]

Identifier: 229916752

GI number: 229916752

Start: 973091

End: 974377

Strand: Reverse

Name: ydfA [H]

Synonym: EAT1b_1025

Alternate gene names: 229916752

Gene position: 974377-973091 (Counterclockwise)

Preceding gene: 229916753

Following gene: 229916751

Centisome position: 32.48

GC content: 52.45

Gene sequence:

>1287_bases
GTGCAGTGGATTGCTAGTTTAATTTTTCTTGTGACGCTTTTCCTCATCATCAAGCAACCGAAAGGCCTCGGCATCGGTTA
CTCGGCGATGGGGATGGCCGTCATCGCCCTCATTTCAGGTGTCGTCAATCTTGATGACGTCGGCACGGTGTGGGGCATCA
TTTGGAACGCGACGTTCACCTTCATCGCGATCATCATCATTTCGCTCATTTTAGACGAGATTGGATTTTTCGAATGGGCC
GCCTTACATATGGCGCGAATCGCCAAAGGTGGCGGGGTGAAGCTCTTCGTCTATATCACGCTACTCGGTGCGCTCGTCGC
GGCTTTGTTTGCGAACGACGGGGCGGCGCTGATCTTGACGCCCATCGTCTTGGCGATGGTCCGGGCACTCAAACTGCCGG
ACGCGGTCGTCTTGGCATATGTCATGGCATCCGGATTCATCGCCGATACGGCGAGTCTCCCGTTCATCGTCTCGAACCTC
GTCAACATCGTTTCAGCCGACTTCTTTGATATCGGCTTCGCCGAGTACGCCGCGCGGATGGTATTTGTGAACGTGTTCGC
GGTCGCGGGGAGTCTGCTCGTGTTGTACCTTTATTTCAAAAAAGATATCCCGAAAAAGTATGACGCCTCACAGTTGGCTG
AACCTTCGTCCGTCATTAAAGACCATACGATGTTCCGCTTATCCTGGATTGTTCTCGGTTTATTGGTCATTGGGTACTTC
ACGAGTGATTTAGTCGAGATCCCCGTTTCGCTCGTCGCGGGTGTAATCGCCTTGTTCTTCCTCGTGATGGCGAACCGCTC
GTCAGCGGTCCAGACGACGCAAGTCATCAAAGGTGCACCGTGGGCCATCGTCTTCTTCTCGCTCGGGATGTATATCGTCG
TCTACGGATTACAAAATGCTGGACTCACACAAGTACTCGCCAACTTCATTGACTGGTTCGCGGTGAACGCCTTCAGTGCC
ACGTTCGGGATGGGATTTTTGGCTGCCATCTTGTCATCGGTCATGAACAACATGCCGACCGTCATGATTGATGCGCTCGC
CATCGACTTGACGCATACGACCGGTCAGATTCGGGAAGCGTTAATCTATGCGAACGTCATTGGTTCCGACCTCGGTCCAA
AGATTACCCCGATTGGGTCGCTAGCGACGCTTCTATGGCTCCACGTGCTCCGCACGAAAGGTCTCAAAATCGGATGGGGT
GAGTATTTCAAAATCGGGATCATCTTGACGATTCCGACATTGGCTATCACGTTGCTCGGCCTATACATCAGCTTATTACT
TTATTGA

Upstream 100 bases:

>100_bases
CGCTCGACGTTGAATCAACAGAAGAGGGAATGACACTCGTGTTGCACGGTTTGCAGCCGAGTTGCTAAAAAATAAATAGA
GATTATTGGAGGATTACATT

Downstream 100 bases:

>100_bases
GGAGTGGATTATATATGAAACTAGTCATCATTGGTTCAGTCGCTGCTGGGACATCGGTTGCGGCGAAAGCGCGTCGGAAC
AACGAAGACGCACAAATCAC

Product: arsenical pump membrane protein

Products: ADP; phosphate; arsenite [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MQWIASLIFLVTLFLIIKQPKGLGIGYSAMGMAVIALISGVVNLDDVGTVWGIIWNATFTFIAIIIISLILDEIGFFEWA
ALHMARIAKGGGVKLFVYITLLGALVAALFANDGAALILTPIVLAMVRALKLPDAVVLAYVMASGFIADTASLPFIVSNL
VNIVSADFFDIGFAEYAARMVFVNVFAVAGSLLVLYLYFKKDIPKKYDASQLAEPSSVIKDHTMFRLSWIVLGLLVIGYF
TSDLVEIPVSLVAGVIALFFLVMANRSSAVQTTQVIKGAPWAIVFFSLGMYIVVYGLQNAGLTQVLANFIDWFAVNAFSA
TFGMGFLAAILSSVMNNMPTVMIDALAIDLTHTTGQIREALIYANVIGSDLGPKITPIGSLATLLWLHVLRTKGLKIGWG
EYFKIGIILTIPTLAITLLGLYISLLLY

Sequences:

>Translated_428_residues
MQWIASLIFLVTLFLIIKQPKGLGIGYSAMGMAVIALISGVVNLDDVGTVWGIIWNATFTFIAIIIISLILDEIGFFEWA
ALHMARIAKGGGVKLFVYITLLGALVAALFANDGAALILTPIVLAMVRALKLPDAVVLAYVMASGFIADTASLPFIVSNL
VNIVSADFFDIGFAEYAARMVFVNVFAVAGSLLVLYLYFKKDIPKKYDASQLAEPSSVIKDHTMFRLSWIVLGLLVIGYF
TSDLVEIPVSLVAGVIALFFLVMANRSSAVQTTQVIKGAPWAIVFFSLGMYIVVYGLQNAGLTQVLANFIDWFAVNAFSA
TFGMGFLAAILSSVMNNMPTVMIDALAIDLTHTTGQIREALIYANVIGSDLGPKITPIGSLATLLWLHVLRTKGLKIGWG
EYFKIGIILTIPTLAITLLGLYISLLLY
>Mature_428_residues
MQWIASLIFLVTLFLIIKQPKGLGIGYSAMGMAVIALISGVVNLDDVGTVWGIIWNATFTFIAIIIISLILDEIGFFEWA
ALHMARIAKGGGVKLFVYITLLGALVAALFANDGAALILTPIVLAMVRALKLPDAVVLAYVMASGFIADTASLPFIVSNL
VNIVSADFFDIGFAEYAARMVFVNVFAVAGSLLVLYLYFKKDIPKKYDASQLAEPSSVIKDHTMFRLSWIVLGLLVIGYF
TSDLVEIPVSLVAGVIALFFLVMANRSSAVQTTQVIKGAPWAIVFFSLGMYIVVYGLQNAGLTQVLANFIDWFAVNAFSA
TFGMGFLAAILSSVMNNMPTVMIDALAIDLTHTTGQIREALIYANVIGSDLGPKITPIGSLATLLWLHVLRTKGLKIGWG
EYFKIGIILTIPTLAITLLGLYISLLLY

Specific function: Involved In Arsenical Resistance. Thought To Form The Channel Of An Arsenite Pump (By Similarity). [C]

COG id: COG1055

COG function: function code P; Na+/H+ antiporter NhaD and related arsenite permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ArsB family [H]

Homologues:

Organism=Escherichia coli, GI87082277, Length=423, Percent_Identity=59.5744680851064, Blast_Score=471, Evalue=1e-134,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000802 [H]

Pfam domain/function: PF02040 ArsB [H]

EC number: NA

Molecular weight: Translated: 46228; Mature: 46228

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHC
>Mature Secondary Structure
MQWIASLIFLVTLFLIIKQPKGLGIGYSAMGMAVIALISGVVNLDDVGTVWGIIWNATFT
CHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
FIAIIIISLILDEIGFFEWAALHMARIAKGGGVKLFVYITLLGALVAALFANDGAALILT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHH
PIVLAMVRALKLPDAVVLAYVMASGFIADTASLPFIVSNLVNIVSADFFDIGFAEYAARM
HHHHHHHHHHCCCHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VFVNVFAVAGSLLVLYLYFKKDIPKKYDASQLAEPSSVIKDHTMFRLSWIVLGLLVIGYF
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TSDLVEIPVSLVAGVIALFFLVMANRSSAVQTTQVIKGAPWAIVFFSLGMYIVVYGLQNA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCC
GLTQVLANFIDWFAVNAFSATFGMGFLAAILSSVMNNMPTVMIDALAIDLTHTTGQIREA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHH
LIYANVIGSDLGPKITPIGSLATLLWLHVLRTKGLKIGWGEYFKIGIILTIPTLAITLLG
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCEECCCCCEEEHHHHHHHHHHHHHHH
LYISLLLY
HHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arsenite [Cytoplasm]; H2O [C]

Specific reaction: ATP + arsenite [Cytoplasm] + H2O = ADP + phosphate + arsenite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]