Click here to switch to the map view.

The map label for this gene is nos [H]

Identifier: 229916746

GI number: 229916746

Start: 962375

End: 963451

Strand: Reverse

Name: nos [H]

Synonym: EAT1b_1019

Alternate gene names: 229916746

Gene position: 963451-962375 (Counterclockwise)

Preceding gene: 229916747

Following gene: 229916745

Centisome position: 32.12

GC content: 55.25

Gene sequence:

>1077_bases
ATGAATCTGTATGAACAAGCGAGCGACTGGATGCGGCAATGGCATGACGACCCGTCCGAACGCCTCCGACAAATCAAAGA
TGAAATTGAGCGCACGGGCACCTATTCGTTGACGACGGAAGAGTTGTGTGAGGGTGCCAAGGTGGCGTGGCGGAATTCGA
ATCGTTGCATCGGCCGCTTGTTTTGGCAGACACTCCATGTGATCGATGCGCGGGACGCCGATACGTTTGAGGCTGTCTAC
GACCATCTTGTCGATCATCTTCGGTTCGCTACGAACGGTGGAAAAATCCGTTCGACGATGACGGTCCTCCCGAACGAGTT
CAAACTATTGAACACACAGTTGATTCGGTACGCGGGATATGAGACACCTGACGGAATCATCGGAGACCCGATGTCCGTCA
CGATGACGAAGCGCGCGAAAGAACTCGGATGGGTCGGAGCCGGCACGCCATTTGATATCCTCCCGCTCATGATCACGGAC
GGACGAGACGTCCGCTTGTTTGAGTTGCCGAACGAGGCCGTGCTTGAGGTGGACATTCAGCACGAGGATGTGGATCTGTT
TGACGGACGGACCATCAAATGGCACGCGGTCCCGGCGATTGCGGATATGGAACTTGAAATCGGAGGGCTCCGGTTTACCT
CGGTCCCGTTTAATGGCTGGTATATGGAGACGGAAATCGGGGCACGGAACTTGGCCGATACGGACCGTTATGATTTATTG
CCGCTCGTCGGACGGTCACTCGGGCTCGATACGACGAAAGAGCGGTCGCTTTGGCGCGACCGGGCACTCGTCGAATTGAA
TATCGCCGTCCTGCAATCGTTCGAACGGGCAGGTGTGACCATCGTCGACCATCATACGGCCGCCAAACAGTTCAAGCGTT
TCGAAAAAAACGAACGGGACGCGGGGCGTGACGTCACCGGGCAATGGAGTTGGCTCATCCCGCCGCTCTCTCCTGCGACG
ACGCACGTTTTTCACGAACATTACGACAATCAAATCAAGACACCGAACTTGTTCGCTCGAAGTGGATGTCCGTTCTCAGG
GTTTCGTCAGCCGGTAAAAGAGGAATTGAAAGGCTAG

Upstream 100 bases:

>100_bases
TGAGTACCCGACGTGTCGCGTGTGAGAATAGATAGTTGAAACAGGGGTCTCCTTTTCTCTAAGATGGAGAAAGAGAGAAT
TGAGTGAGGAGATCTTAGAT

Downstream 100 bases:

>100_bases
AACCGAGAGAAAGGATGATTGCTGTGGCAGTCCCACTTTGGAAACGAGCCGTCGCTGGTCAAATCGACCAACTCGTCTTT
CAGACACCGGCAAAACTGAT

Product: Nitric-oxide synthase

Products: NA

Alternate protein names: NOSoxy-like protein [H]

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MNLYEQASDWMRQWHDDPSERLRQIKDEIERTGTYSLTTEELCEGAKVAWRNSNRCIGRLFWQTLHVIDARDADTFEAVY
DHLVDHLRFATNGGKIRSTMTVLPNEFKLLNTQLIRYAGYETPDGIIGDPMSVTMTKRAKELGWVGAGTPFDILPLMITD
GRDVRLFELPNEAVLEVDIQHEDVDLFDGRTIKWHAVPAIADMELEIGGLRFTSVPFNGWYMETEIGARNLADTDRYDLL
PLVGRSLGLDTTKERSLWRDRALVELNIAVLQSFERAGVTIVDHHTAAKQFKRFEKNERDAGRDVTGQWSWLIPPLSPAT
THVFHEHYDNQIKTPNLFARSGCPFSGFRQPVKEELKG

Sequences:

>Translated_358_residues
MNLYEQASDWMRQWHDDPSERLRQIKDEIERTGTYSLTTEELCEGAKVAWRNSNRCIGRLFWQTLHVIDARDADTFEAVY
DHLVDHLRFATNGGKIRSTMTVLPNEFKLLNTQLIRYAGYETPDGIIGDPMSVTMTKRAKELGWVGAGTPFDILPLMITD
GRDVRLFELPNEAVLEVDIQHEDVDLFDGRTIKWHAVPAIADMELEIGGLRFTSVPFNGWYMETEIGARNLADTDRYDLL
PLVGRSLGLDTTKERSLWRDRALVELNIAVLQSFERAGVTIVDHHTAAKQFKRFEKNERDAGRDVTGQWSWLIPPLSPAT
THVFHEHYDNQIKTPNLFARSGCPFSGFRQPVKEELKG
>Mature_358_residues
MNLYEQASDWMRQWHDDPSERLRQIKDEIERTGTYSLTTEELCEGAKVAWRNSNRCIGRLFWQTLHVIDARDADTFEAVY
DHLVDHLRFATNGGKIRSTMTVLPNEFKLLNTQLIRYAGYETPDGIIGDPMSVTMTKRAKELGWVGAGTPFDILPLMITD
GRDVRLFELPNEAVLEVDIQHEDVDLFDGRTIKWHAVPAIADMELEIGGLRFTSVPFNGWYMETEIGARNLADTDRYDLL
PLVGRSLGLDTTKERSLWRDRALVELNIAVLQSFERAGVTIVDHHTAAKQFKRFEKNERDAGRDVTGQWSWLIPPLSPAT
THVFHEHYDNQIKTPNLFARSGCPFSGFRQPVKEELKG

Specific function: Catalyzes the production of nitric oxide [H]

COG id: COG4362

COG function: function code PE; Nitric oxide synthase, oxygenase domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NOS family. Bacterial NOS oxygenase subfamily [H]

Homologues:

Organism=Homo sapiens, GI24041029, Length=349, Percent_Identity=45.2722063037249, Blast_Score=322, Evalue=3e-88,
Organism=Homo sapiens, GI10835173, Length=326, Percent_Identity=44.7852760736196, Blast_Score=306, Evalue=3e-83,
Organism=Homo sapiens, GI231571329, Length=350, Percent_Identity=42, Blast_Score=300, Evalue=1e-81,
Organism=Homo sapiens, GI231571314, Length=350, Percent_Identity=42, Blast_Score=300, Evalue=1e-81,
Organism=Homo sapiens, GI231571356, Length=350, Percent_Identity=42, Blast_Score=300, Evalue=1e-81,
Organism=Homo sapiens, GI40254422, Length=350, Percent_Identity=42, Blast_Score=300, Evalue=1e-81,
Organism=Drosophila melanogaster, GI78706866, Length=340, Percent_Identity=42.3529411764706, Blast_Score=301, Evalue=5e-82,
Organism=Drosophila melanogaster, GI78706882, Length=340, Percent_Identity=42.3529411764706, Blast_Score=301, Evalue=5e-82,
Organism=Drosophila melanogaster, GI78706868, Length=340, Percent_Identity=42.3529411764706, Blast_Score=301, Evalue=6e-82,
Organism=Drosophila melanogaster, GI78706870, Length=340, Percent_Identity=42.3529411764706, Blast_Score=301, Evalue=6e-82,
Organism=Drosophila melanogaster, GI78706878, Length=340, Percent_Identity=42.3529411764706, Blast_Score=301, Evalue=6e-82,
Organism=Drosophila melanogaster, GI78706880, Length=340, Percent_Identity=42.3529411764706, Blast_Score=301, Evalue=6e-82,
Organism=Drosophila melanogaster, GI24583543, Length=340, Percent_Identity=42.3529411764706, Blast_Score=300, Evalue=9e-82,
Organism=Drosophila melanogaster, GI78706876, Length=340, Percent_Identity=42.3529411764706, Blast_Score=300, Evalue=9e-82,
Organism=Drosophila melanogaster, GI78706872, Length=150, Percent_Identity=40.6666666666667, Blast_Score=122, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017142
- InterPro:   IPR004030 [H]

Pfam domain/function: PF02898 NO_synthase [H]

EC number: NA

Molecular weight: Translated: 41005; Mature: 41005

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS60001 NOS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLYEQASDWMRQWHDDPSERLRQIKDEIERTGTYSLTTEELCEGAKVAWRNSNRCIGRL
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCEEEECCCCCHHHHH
FWQTLHVIDARDADTFEAVYDHLVDHLRFATNGGKIRSTMTVLPNEFKLLNTQLIRYAGY
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHCCC
ETPDGIIGDPMSVTMTKRAKELGWVGAGTPFDILPLMITDGRDVRLFELPNEAVLEVDIQ
CCCCCCCCCCCEEHHHHHHHHHCCCCCCCCHHEEEEEEECCCCEEEEECCCCEEEEEEEC
HEDVDLFDGRTIKWHAVPAIADMELEIGGLRFTSVPFNGWYMETEIGARNLADTDRYDLL
CCCEEEECCCEEEEEECCCEEECEEEECCEEEEECCCCCEEEEECCCCCCCCCCCCHHHH
PLVGRSLGLDTTKERSLWRDRALVELNIAVLQSFERAGVTIVDHHTAAKQFKRFEKNERD
HHHHHHCCCCCHHHHHHHHCCCEEEEEHHHHHHHHHCCCEEEECHHHHHHHHHHHCCCCC
AGRDVTGQWSWLIPPLSPATTHVFHEHYDNQIKTPNLFARSGCPFSGFRQPVKEELKG
CCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MNLYEQASDWMRQWHDDPSERLRQIKDEIERTGTYSLTTEELCEGAKVAWRNSNRCIGRL
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCEEEECCCCCHHHHH
FWQTLHVIDARDADTFEAVYDHLVDHLRFATNGGKIRSTMTVLPNEFKLLNTQLIRYAGY
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHCCC
ETPDGIIGDPMSVTMTKRAKELGWVGAGTPFDILPLMITDGRDVRLFELPNEAVLEVDIQ
CCCCCCCCCCCEEHHHHHHHHHCCCCCCCCHHEEEEEEECCCCEEEEECCCCEEEEEEEC
HEDVDLFDGRTIKWHAVPAIADMELEIGGLRFTSVPFNGWYMETEIGARNLADTDRYDLL
CCCEEEECCCEEEEEECCCEEECEEEECCEEEEECCCCCEEEEECCCCCCCCCCCCHHHH
PLVGRSLGLDTTKERSLWRDRALVELNIAVLQSFERAGVTIVDHHTAAKQFKRFEKNERD
HHHHHHCCCCCHHHHHHHHCCCEEEEEHHHHHHHHHCCCEEEECHHHHHHHHHHHCCCCC
AGRDVTGQWSWLIPPLSPATTHVFHEHYDNQIKTPNLFARSGCPFSGFRQPVKEELKG
CCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377; 11856757; 12220171 [H]