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The map label for this gene is ribE [H]

Identifier: 229916730

GI number: 229916730

Start: 946983

End: 947624

Strand: Reverse

Name: ribE [H]

Synonym: EAT1b_1003

Alternate gene names: 229916730

Gene position: 947624-946983 (Counterclockwise)

Preceding gene: 229916731

Following gene: 229916729

Centisome position: 31.59

GC content: 52.96

Gene sequence:

>642_bases
ATGTTCACGGGAATCGTAGAAGAAATCGGATCGGTCAAAGCGGTCAAGCGCAACGGACCGTCTTTACGACTCGTCTTGTC
CGCTGTGAAAGTGCTTGACGATGTGAAGCTCGGGGACAGCATAGCCGTGAATGGGATCTGCCTCACGGTGACGACGTTCG
ATGACGCGACGTTCTCAGTCGATGTCATGCCAGAAACGTTGAAGGCCTCGAGCCTAGATGGGGTCAAGCCGGGAACACAA
GTCAATCTCGAACGGGCGATGCCGGCGAACGGACGGTTTGGTGGACATTTCGTATCCGGACATGTCGACGGGGTCGGGCG
CATCACGAAGAAACGACCGCTCGCGAATGCCGTCTATATCGACATCCAATGTGATCGCGCGCTCCTTTCCTACATCGTCC
CGAAAGGTTCGATATGTGTAGATGGGACGAGTTTGACCGTGTTTGACGTGTCGGAACGTGGGTTTACCTTGTCCTTGATC
CCGCACACGTACAGCGAGACCGTGCTTGGGATGAAACAGACGGGTGATTTAGTCAATTTAGAATGTGATCTGTTAGCAAA
ATATATGGAAAAGGTCGTCGGGCAGTCGAAGATGACGCTCGAGTCCTTGACAGAACAAGGATATGGAAAGGCGGGATGGT
AA

Upstream 100 bases:

>100_bases
AGCAGGAAGGGACGATCGATGGGATATCCCGTTTTACAATCCATCAGGTTGAAACGATTGGTCCCGACCTAAAAGTCGTC
CTTCGAAAGGGGGCGCTCTG

Downstream 100 bases:

>100_bases
TGTTTGATTCGATTGAACTGGCGATTGAGGAACTGAGACGAGGGCGTCCCATCATCGTCGTGGATGACGAGGACCGTGAG
AATGAAGGGGACTTTGTCGT

Product: riboflavin synthase, subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MFTGIVEEIGSVKAVKRNGPSLRLVLSAVKVLDDVKLGDSIAVNGICLTVTTFDDATFSVDVMPETLKASSLDGVKPGTQ
VNLERAMPANGRFGGHFVSGHVDGVGRITKKRPLANAVYIDIQCDRALLSYIVPKGSICVDGTSLTVFDVSERGFTLSLI
PHTYSETVLGMKQTGDLVNLECDLLAKYMEKVVGQSKMTLESLTEQGYGKAGW

Sequences:

>Translated_213_residues
MFTGIVEEIGSVKAVKRNGPSLRLVLSAVKVLDDVKLGDSIAVNGICLTVTTFDDATFSVDVMPETLKASSLDGVKPGTQ
VNLERAMPANGRFGGHFVSGHVDGVGRITKKRPLANAVYIDIQCDRALLSYIVPKGSICVDGTSLTVFDVSERGFTLSLI
PHTYSETVLGMKQTGDLVNLECDLLAKYMEKVVGQSKMTLESLTEQGYGKAGW
>Mature_213_residues
MFTGIVEEIGSVKAVKRNGPSLRLVLSAVKVLDDVKLGDSIAVNGICLTVTTFDDATFSVDVMPETLKASSLDGVKPGTQ
VNLERAMPANGRFGGHFVSGHVDGVGRITKKRPLANAVYIDIQCDRALLSYIVPKGSICVDGTSLTVFDVSERGFTLSLI
PHTYSETVLGMKQTGDLVNLECDLLAKYMEKVVGQSKMTLESLTEQGYGKAGW

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats [H]

Homologues:

Organism=Escherichia coli, GI1787952, Length=188, Percent_Identity=39.8936170212766, Blast_Score=114, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6319733, Length=214, Percent_Identity=44.8598130841121, Blast_Score=165, Evalue=4e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001783
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00677 Lum_binding [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 22832; Mature: 22832

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00693 LUM_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTGIVEEIGSVKAVKRNGPSLRLVLSAVKVLDDVKLGDSIAVNGICLTVTTFDDATFSV
CCCCHHHHHCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEECCEEEEEEEECCCEEEE
DVMPETLKASSLDGVKPGTQVNLERAMPANGRFGGHFVSGHVDGVGRITKKRPLANAVYI
EECCHHHCCCCCCCCCCCCEEEHHHCCCCCCCCCCEEEECCCCCCCCHHCCCCCCEEEEE
DIQCDRALLSYIVPKGSICVDGTSLTVFDVSERGFTLSLIPHTYSETVLGMKQTGDLVNL
EEEECHHHHHHHCCCCCEEECCCEEEEEEECCCCEEEEECCCCCCHHHCCHHCCCCEEEC
ECDLLAKYMEKVVGQSKMTLESLTEQGYGKAGW
CHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MFTGIVEEIGSVKAVKRNGPSLRLVLSAVKVLDDVKLGDSIAVNGICLTVTTFDDATFSV
CCCCHHHHHCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEECCEEEEEEEECCCEEEE
DVMPETLKASSLDGVKPGTQVNLERAMPANGRFGGHFVSGHVDGVGRITKKRPLANAVYI
EECCHHHCCCCCCCCCCCCEEEHHHCCCCCCCCCCEEEECCCCCCCCHHCCCCCCEEEEE
DIQCDRALLSYIVPKGSICVDGTSLTVFDVSERGFTLSLIPHTYSETVLGMKQTGDLVNL
EEEECHHHHHHHCCCCCEEECCCEEEEEEECCCCEEEEECCCCCCHHHCCHHCCCCEEEC
ECDLLAKYMEKVVGQSKMTLESLTEQGYGKAGW
CHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9297088 [H]