Click here to switch to the map view.

The map label for this gene is yaaJ [C]

Identifier: 229916723

GI number: 229916723

Start: 939119

End: 940480

Strand: Reverse

Name: yaaJ [C]

Synonym: EAT1b_0996

Alternate gene names: 229916723

Gene position: 940480-939119 (Counterclockwise)

Preceding gene: 229916724

Following gene: 229916721

Centisome position: 31.35

GC content: 52.06

Gene sequence:

>1362_bases
GTGAATCAAATTGAAAGTTGGCTGACGAGTTTATCCAATCTCGTATGGGGGCCACATTTACTCATATTACTTGTCGGGAC
AGGGATTTTCTTGACAGTCCGTCTCGGATTTATCCAAGTGACGCGACTTGGGGTCGGTCTGAAAGAGGCATTTATACCAA
AACCAGAACAACCGAAGAAAAAGCAACGTGGGGACATCTCGCATTTCCAAGCACTTATGACGGCATTGGCGGCCACGGTC
GGTACCGGAAACATGGTCGGTGTCGCGACGGCGATTGTCCTCGGGGGACCGGGTGCTGTCGTTTGGATGTGGTTATCTGG
TTTCGTCGGGATGGCGACGAAATATGCGGAGGCCATCCTTGCCGTCAAATATCGTGTCGTCGATGAACGAGGCGAAATTG
CCGGGGGTCCGATGTACTACTTAGAACACGGCTTGAAGAAAAAATGGCTCGGCGTCTTGTTCGCCGTCTTTGCATCGGTC
GCAGCCTTTGGGATTGGGAACGGAGTCCAGACGAACTCGATCAGTCTCGCCTTGAACAATTCGTTCAACGTCCCAATGGT
TGTGACAGGGATCGTCATGATGGCGGTCACGGCATTCGTCATCTTAGGTGGTGTCAAATCAATCGGGAAAGTCGTCGGAA
TCTTCGTCCCGTTCATGATTTTCGCATATGTCGGGAGCGGGCTCGTGATTCTCATCATGAACCTTGAATTGGTACCAGGT
GCCTTGTCGACGATGTTCTCGAGCACATTCAGTGCCGAAGCCATCGGTGGGGGTGCATTAGGTGCCGCGATTCGCTACGG
GGTCGCGCGCGGTGTGTTCTCTAACGAGGCAGGACTTGGATCTGCACCGATTGCGGCCGCGGCAGCGAAGACCGACTTCC
CGGGACGTCAAGCACTCGTCTCGATGACACAAGTGTTGATTGACACACTCATCGTCTGTTCTATCACGGGACTCACGATC
GTTATGGGTGGTCAATATACGGAAGGGCTTGAAGGGATTGCCTTGACGCAAGCCTCGTTCGCTTACTTCTTAGGACCTAT
TGGCGAAATCATCGTGACGATCTCGCTTGTCTTCTTCGCCTATTCGACCGTCCTCGGTTGGTGTTATTACGGTGAACGTT
CAGCGTATTATTTACTCGGGGAAAAAGTGATCGTCCCGTATCGTGTCCTCTACATTTTAGTCGCGGGACTCGGTGCGATG
ACGACGAACTTGAATTTGATCTGGTCGATCTCGGATGTGTTCAACGGTCTCATGGCTATCCCGAACTTGATTGGACTCTT
GTTTCTCTCAGGTGTGGTCGTCGCCGAGACAAAACGATTCGAACTCGAACGGAAAAAAGAACGGCAGTCATCTGCCGCTT
GA

Upstream 100 bases:

>100_bases
AAAGCGTTTTCTTAATATAGGCTACCTTGACTCGATTGTGAATGTTCTGAAAAATATAGGGAGAGCGCTCAAATCAAGAA
CGTAGGAGGAGAGCAACACG

Downstream 100 bases:

>100_bases
TGAGAAAACCCGCCGGACTACACGTGTCGGCGGGTTTTTGTCATACCTTGAATTGGCGAATCAGTGAATCAAGTTCTTCC
ATCTTTTGTTGCGACTGTTC

Product: amino acid carrier protein

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MNQIESWLTSLSNLVWGPHLLILLVGTGIFLTVRLGFIQVTRLGVGLKEAFIPKPEQPKKKQRGDISHFQALMTALAATV
GTGNMVGVATAIVLGGPGAVVWMWLSGFVGMATKYAEAILAVKYRVVDERGEIAGGPMYYLEHGLKKKWLGVLFAVFASV
AAFGIGNGVQTNSISLALNNSFNVPMVVTGIVMMAVTAFVILGGVKSIGKVVGIFVPFMIFAYVGSGLVILIMNLELVPG
ALSTMFSSTFSAEAIGGGALGAAIRYGVARGVFSNEAGLGSAPIAAAAAKTDFPGRQALVSMTQVLIDTLIVCSITGLTI
VMGGQYTEGLEGIALTQASFAYFLGPIGEIIVTISLVFFAYSTVLGWCYYGERSAYYLLGEKVIVPYRVLYILVAGLGAM
TTNLNLIWSISDVFNGLMAIPNLIGLLFLSGVVVAETKRFELERKKERQSSAA

Sequences:

>Translated_453_residues
MNQIESWLTSLSNLVWGPHLLILLVGTGIFLTVRLGFIQVTRLGVGLKEAFIPKPEQPKKKQRGDISHFQALMTALAATV
GTGNMVGVATAIVLGGPGAVVWMWLSGFVGMATKYAEAILAVKYRVVDERGEIAGGPMYYLEHGLKKKWLGVLFAVFASV
AAFGIGNGVQTNSISLALNNSFNVPMVVTGIVMMAVTAFVILGGVKSIGKVVGIFVPFMIFAYVGSGLVILIMNLELVPG
ALSTMFSSTFSAEAIGGGALGAAIRYGVARGVFSNEAGLGSAPIAAAAAKTDFPGRQALVSMTQVLIDTLIVCSITGLTI
VMGGQYTEGLEGIALTQASFAYFLGPIGEIIVTISLVFFAYSTVLGWCYYGERSAYYLLGEKVIVPYRVLYILVAGLGAM
TTNLNLIWSISDVFNGLMAIPNLIGLLFLSGVVVAETKRFELERKKERQSSAA
>Mature_453_residues
MNQIESWLTSLSNLVWGPHLLILLVGTGIFLTVRLGFIQVTRLGVGLKEAFIPKPEQPKKKQRGDISHFQALMTALAATV
GTGNMVGVATAIVLGGPGAVVWMWLSGFVGMATKYAEAILAVKYRVVDERGEIAGGPMYYLEHGLKKKWLGVLFAVFASV
AAFGIGNGVQTNSISLALNNSFNVPMVVTGIVMMAVTAFVILGGVKSIGKVVGIFVPFMIFAYVGSGLVILIMNLELVPG
ALSTMFSSTFSAEAIGGGALGAAIRYGVARGVFSNEAGLGSAPIAAAAAKTDFPGRQALVSMTQVLIDTLIVCSITGLTI
VMGGQYTEGLEGIALTQASFAYFLGPIGEIIVTISLVFFAYSTVLGWCYYGERSAYYLLGEKVIVPYRVLYILVAGLGAM
TTNLNLIWSISDVFNGLMAIPNLIGLLFLSGVVVAETKRFELERKKERQSSAA

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=448, Percent_Identity=37.5, Blast_Score=266, Evalue=3e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 47984; Mature: 47984

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQIESWLTSLSNLVWGPHLLILLVGTGIFLTVRLGFIQVTRLGVGLKEAFIPKPEQPKK
CCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCH
KQRGDISHFQALMTALAATVGTGNMVGVATAIVLGGPGAVVWMWLSGFVGMATKYAEAIL
HHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHH
AVKYRVVDERGEIAGGPMYYLEHGLKKKWLGVLFAVFASVAAFGIGNGVQTNSISLALNN
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECC
SFNVPMVVTGIVMMAVTAFVILGGVKSIGKVVGIFVPFMIFAYVGSGLVILIMNLELVPG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHCCH
ALSTMFSSTFSAEAIGGGALGAAIRYGVARGVFSNEAGLGSAPIAAAAAKTDFPGRQALV
HHHHHHHCCCCHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCHHHHHH
SMTQVLIDTLIVCSITGLTIVMGGQYTEGLEGIALTQASFAYFLGPIGEIIVTISLVFFA
HHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHH
YSTVLGWCYYGERSAYYLLGEKVIVPYRVLYILVAGLGAMTTNLNLIWSISDVFNGLMAI
HHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHH
PNLIGLLFLSGVVVAETKRFELERKKERQSSAA
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNQIESWLTSLSNLVWGPHLLILLVGTGIFLTVRLGFIQVTRLGVGLKEAFIPKPEQPKK
CCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCH
KQRGDISHFQALMTALAATVGTGNMVGVATAIVLGGPGAVVWMWLSGFVGMATKYAEAIL
HHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHH
AVKYRVVDERGEIAGGPMYYLEHGLKKKWLGVLFAVFASVAAFGIGNGVQTNSISLALNN
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECC
SFNVPMVVTGIVMMAVTAFVILGGVKSIGKVVGIFVPFMIFAYVGSGLVILIMNLELVPG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHCCH
ALSTMFSSTFSAEAIGGGALGAAIRYGVARGVFSNEAGLGSAPIAAAAAKTDFPGRQALV
HHHHHHHCCCCHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCHHHHHH
SMTQVLIDTLIVCSITGLTIVMGGQYTEGLEGIALTQASFAYFLGPIGEIIVTISLVFFA
HHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHH
YSTVLGWCYYGERSAYYLLGEKVIVPYRVLYILVAGLGAMTTNLNLIWSISDVFNGLMAI
HHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHH
PNLIGLLFLSGVVVAETKRFELERKKERQSSAA
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]