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The map label for this gene is smpB

Identifier: 229916579

GI number: 229916579

Start: 803254

End: 803724

Strand: Reverse

Name: smpB

Synonym: EAT1b_0851

Alternate gene names: 229916579

Gene position: 803724-803254 (Counterclockwise)

Preceding gene: 229916580

Following gene: 229916578

Centisome position: 26.79

GC content: 53.29

Gene sequence:

>471_bases
ATGGCCAAGAAGAAAGATTCGAACGCACTCGCCCAAAACCGCAAGGCGTCCTTCGATTACGCGATCGAAGATACGATTGA
GGCGGGCATGGTGCTCACAGGGACGGAGATCAAATCGGTCCGTCAATCGAAGATCAACATCGCGGATGCGTACGTCCGGT
TCGATGGGGGCGAGGCGACGCTTCATAACTGTCATATCAGCCCATTCGAACAGGGGAACCGCTTCAACCATGACCCACTC
CGCGTCCGAAAGCTATTGTTGCATAAGAAGCAAATCAATCAGCTCATTGGAGCATCAGGGCGTGACGGCTACACGATCAT
CCCGCTCAAAGTGTATATCAAGAACGGGTTCGCCAAGTGCCTGCTCGGCGTCGGAAAAGGGAAGAAGAAGTACGACAAAC
GTGAGGACTTGAAGAAGAAAGACGCGAAGCGTGACGTCGACCGTGCGATGCGCGATCGGCAAAAATACTGA

Upstream 100 bases:

>100_bases
CAAACGAAAAGGCGACGCCAAACGAGGTGCGAAGAAACGTCGCTGACGGGGAAGGGCTCATCCCTTCCTCCGTATCTTGT
AGATAAAGGACTGAATGTGT

Downstream 100 bases:

>100_bases
CAACGGATCCTAATTGTGGTATATTAAATAGAGTACCAATTACCACCTCGGGGACGTTATGGATTCGACGGGGGTAGGTC
GGTCGTATGTGGCGTGTCGA

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 156; Mature: 155

Protein sequence:

>156_residues
MAKKKDSNALAQNRKASFDYAIEDTIEAGMVLTGTEIKSVRQSKINIADAYVRFDGGEATLHNCHISPFEQGNRFNHDPL
RVRKLLLHKKQINQLIGASGRDGYTIIPLKVYIKNGFAKCLLGVGKGKKKYDKREDLKKKDAKRDVDRAMRDRQKY

Sequences:

>Translated_156_residues
MAKKKDSNALAQNRKASFDYAIEDTIEAGMVLTGTEIKSVRQSKINIADAYVRFDGGEATLHNCHISPFEQGNRFNHDPL
RVRKLLLHKKQINQLIGASGRDGYTIIPLKVYIKNGFAKCLLGVGKGKKKYDKREDLKKKDAKRDVDRAMRDRQKY
>Mature_155_residues
AKKKDSNALAQNRKASFDYAIEDTIEAGMVLTGTEIKSVRQSKINIADAYVRFDGGEATLHNCHISPFEQGNRFNHDPLR
VRKLLLHKKQINQLIGASGRDGYTIIPLKVYIKNGFAKCLLGVGKGKKKYDKREDLKKKDAKRDVDRAMRDRQKY

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=152, Percent_Identity=41.4473684210526, Blast_Score=125, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_EXISA (C4L5G5)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002885225.1
- GeneID:   7869016
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_0851
- OMA:   EKYEAGI
- ProtClustDB:   PRK05422
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 17753; Mature: 17622

Theoretical pI: Translated: 10.52; Mature: 10.52

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKKDSNALAQNRKASFDYAIEDTIEAGMVLTGTEIKSVRQSKINIADAYVRFDGGEAT
CCCCCCCHHHHHCCCCCCCHHHHHHHHCCEEEECHHHHHHHHHHCCEEEEEEEECCCCEE
LHNCHISPFEQGNRFNHDPLRVRKLLLHKKQINQLIGASGRDGYTIIPLKVYIKNGFAKC
EEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCHHHH
LLGVGKGKKKYDKREDLKKKDAKRDVDRAMRDRQKY
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKKKDSNALAQNRKASFDYAIEDTIEAGMVLTGTEIKSVRQSKINIADAYVRFDGGEAT
CCCCCCHHHHHCCCCCCCHHHHHHHHCCEEEECHHHHHHHHHHCCEEEEEEEECCCCEE
LHNCHISPFEQGNRFNHDPLRVRKLLLHKKQINQLIGASGRDGYTIIPLKVYIKNGFAKC
EEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCHHHH
LLGVGKGKKKYDKREDLKKKDAKRDVDRAMRDRQKY
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA