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The map label for this gene is yflA [H]

Identifier: 229916525

GI number: 229916525

Start: 749361

End: 750743

Strand: Direct

Name: yflA [H]

Synonym: EAT1b_0795

Alternate gene names: 229916525

Gene position: 749361-750743 (Clockwise)

Preceding gene: 229916516

Following gene: 229916540

Centisome position: 24.98

GC content: 52.35

Gene sequence:

>1383_bases
ATGAATGATATTATTAATCTTGTCACGAAGGTCTCCGACTGGCTTTGGGGCGTGCCGATTATCTCGTTACTCGTCTTGTC
CGGTATCTTTCTCACCATCAAGCTCGGTTTCTTTCAGTTTCGTTACCCCATCTACATATTCAAGCAAACGTTCGGCAGTA
TCTTCAAAAAGCCGAAAGGTGAAGGTGATATCAGTCCGAGACAAGCGTTGACATCAGCCCTCTCTTCAACAGTCGGGGCC
GCGAACATCATCGGTGTACCTGCAGCGATCATGTTCGGGGGACCGGGTGCCATCTTCTGGATGTGGGTCATCGCTATCAT
CGGCATGGCCATCAAGTTTTCAGAGAGTGTGCTGGCCGTCCACTACCGTGAAAAAAATGACGCTGGCGAATTTGTCGGGG
GACCGGTCTACTACATATCAAAAGGACTCCGTTGGAAATGGCTCGCGACCTGGTTCGCCTTCGCCCTCATGATCGAACTC
ATTCCGAGTATCATGGTACAAGGGAACGCCGTCAGCTCGGCAGTCCGTGAGACGTTCAATGGGAACACGCTCATCACCGG
TATCGTCGTCGCTATTCTTGTTGGCATCATCGTCTTTGGTGGACTGAAGCGCATCGCGAAAGTCACGGAGATCGTCGTTC
CTGGCATGGCACTCGTCTATGTCGGAGCCGGAATCGTCATCGTCTTGATGAACTTCTCTGAGATTCCAAACGTGCTCAGT
CTCATCTTCTCGTATGCGTTCGAGCCGATGGCCGCGTTCGGAGGATTTGCCGGAGCTGCCGTCGCCGAGACGATTCGTTG
GGGCTTCGCCCGTGGACTCTATTCTAACGAAGCGGGTCTTGGAACTGCACCGATTGCCCACGCCGCTGCACAAACGGATC
ACCCAGTGCGCCAAGGATTCTGGGCGGTCATCGGTATCGTCATCGATACGCTCATCATTTGTAGCACGACCGCCTTCGTC
GTCTTGTCTTCCGGTGTGTGGACGGCAGACGGAGCGATGGATGACCCAGCGGCGTTGACGACGATCGCCTTCCGTGAATA
CTTCGGTTACACGGGAAGCTTACTCGTCACCATCTCGCTCATCTTCTTCGTTCTCTCAACCATTATCGTCATCATCTTTT
ACGGCTCGCGACAAGCCGAGTACTTGTTCGGTTTGACGGCAGGGAAAATCATGAAGGCCGTCTATATCGTTTCGATTGTC
ATCGGTGCAATCGGAGGCGCCCAAATGATTTGGAACTTCCTCGACTTGATGCTCGCGATGATCCTCATCCCGAACATGAT
TGCCGTCCTGCTTCTAAGTGGTGAAGTGAAACGATTGACGACCGAATTCTTCACATCCGAGAAGTACTATAAGAAAGATA
TCGCCGAAGAAGAGGCGTCTTAA

Upstream 100 bases:

>100_bases
ACTCTCATTCTATTTTCCTCCTTTATTTTGTCAAAGATGTTTAGAGGTCCTTACTTTAGGAAACAGTATTTAAGTAAAAT
AATGGAATTGGAGTGGTTAT

Downstream 100 bases:

>100_bases
CCAACAATCCGACTGCTTAGGCAGTCGGATCTTTTTTAGGATGCAGGTTCGACTTTCTCGATTCCCGCCTTGATGGCTTG
TACCGAGGCGTCAAACTTCT

Product: amino acid carrier protein

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MNDIINLVTKVSDWLWGVPIISLLVLSGIFLTIKLGFFQFRYPIYIFKQTFGSIFKKPKGEGDISPRQALTSALSSTVGA
ANIIGVPAAIMFGGPGAIFWMWVIAIIGMAIKFSESVLAVHYREKNDAGEFVGGPVYYISKGLRWKWLATWFAFALMIEL
IPSIMVQGNAVSSAVRETFNGNTLITGIVVAILVGIIVFGGLKRIAKVTEIVVPGMALVYVGAGIVIVLMNFSEIPNVLS
LIFSYAFEPMAAFGGFAGAAVAETIRWGFARGLYSNEAGLGTAPIAHAAAQTDHPVRQGFWAVIGIVIDTLIICSTTAFV
VLSSGVWTADGAMDDPAALTTIAFREYFGYTGSLLVTISLIFFVLSTIIVIIFYGSRQAEYLFGLTAGKIMKAVYIVSIV
IGAIGGAQMIWNFLDLMLAMILIPNMIAVLLLSGEVKRLTTEFFTSEKYYKKDIAEEEAS

Sequences:

>Translated_460_residues
MNDIINLVTKVSDWLWGVPIISLLVLSGIFLTIKLGFFQFRYPIYIFKQTFGSIFKKPKGEGDISPRQALTSALSSTVGA
ANIIGVPAAIMFGGPGAIFWMWVIAIIGMAIKFSESVLAVHYREKNDAGEFVGGPVYYISKGLRWKWLATWFAFALMIEL
IPSIMVQGNAVSSAVRETFNGNTLITGIVVAILVGIIVFGGLKRIAKVTEIVVPGMALVYVGAGIVIVLMNFSEIPNVLS
LIFSYAFEPMAAFGGFAGAAVAETIRWGFARGLYSNEAGLGTAPIAHAAAQTDHPVRQGFWAVIGIVIDTLIICSTTAFV
VLSSGVWTADGAMDDPAALTTIAFREYFGYTGSLLVTISLIFFVLSTIIVIIFYGSRQAEYLFGLTAGKIMKAVYIVSIV
IGAIGGAQMIWNFLDLMLAMILIPNMIAVLLLSGEVKRLTTEFFTSEKYYKKDIAEEEAS
>Mature_460_residues
MNDIINLVTKVSDWLWGVPIISLLVLSGIFLTIKLGFFQFRYPIYIFKQTFGSIFKKPKGEGDISPRQALTSALSSTVGA
ANIIGVPAAIMFGGPGAIFWMWVIAIIGMAIKFSESVLAVHYREKNDAGEFVGGPVYYISKGLRWKWLATWFAFALMIEL
IPSIMVQGNAVSSAVRETFNGNTLITGIVVAILVGIIVFGGLKRIAKVTEIVVPGMALVYVGAGIVIVLMNFSEIPNVLS
LIFSYAFEPMAAFGGFAGAAVAETIRWGFARGLYSNEAGLGTAPIAHAAAQTDHPVRQGFWAVIGIVIDTLIICSTTAFV
VLSSGVWTADGAMDDPAALTTIAFREYFGYTGSLLVTISLIFFVLSTIIVIIFYGSRQAEYLFGLTAGKIMKAVYIVSIV
IGAIGGAQMIWNFLDLMLAMILIPNMIAVLLLSGEVKRLTTEFFTSEKYYKKDIAEEEAS

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=457, Percent_Identity=36.3238512035011, Blast_Score=234, Evalue=7e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 49774; Mature: 49774

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: PS00873 NA_ALANINE_SYMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDIINLVTKVSDWLWGVPIISLLVLSGIFLTIKLGFFQFRYPIYIFKQTFGSIFKKPKG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EGDISPRQALTSALSSTVGAANIIGVPAAIMFGGPGAIFWMWVIAIIGMAIKFSESVLAV
CCCCCHHHHHHHHHHHHCCCHHHHCCCHHEEECCCHHHHHHHHHHHHHHHHHHCCCEEEE
HYREKNDAGEFVGGPVYYISKGLRWKWLATWFAFALMIELIPSIMVQGNAVSSAVRETFN
EEECCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
GNTLITGIVVAILVGIIVFGGLKRIAKVTEIVVPGMALVYVGAGIVIVLMNFSEIPNVLS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEEECCHHHHHHHHH
LIFSYAFEPMAAFGGFAGAAVAETIRWGFARGLYSNEAGLGTAPIAHAAAQTDHPVRQGF
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCHHHHHH
WAVIGIVIDTLIICSTTAFVVLSSGVWTADGAMDDPAALTTIAFREYFGYTGSLLVTISL
HHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
IFFVLSTIIVIIFYGSRQAEYLFGLTAGKIMKAVYIVSIVIGAIGGAQMIWNFLDLMLAM
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
ILIPNMIAVLLLSGEVKRLTTEFFTSEKYYKKDIAEEEAS
HHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHCCCC
>Mature Secondary Structure
MNDIINLVTKVSDWLWGVPIISLLVLSGIFLTIKLGFFQFRYPIYIFKQTFGSIFKKPKG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EGDISPRQALTSALSSTVGAANIIGVPAAIMFGGPGAIFWMWVIAIIGMAIKFSESVLAV
CCCCCHHHHHHHHHHHHCCCHHHHCCCHHEEECCCHHHHHHHHHHHHHHHHHHCCCEEEE
HYREKNDAGEFVGGPVYYISKGLRWKWLATWFAFALMIELIPSIMVQGNAVSSAVRETFN
EEECCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
GNTLITGIVVAILVGIIVFGGLKRIAKVTEIVVPGMALVYVGAGIVIVLMNFSEIPNVLS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEEECCHHHHHHHHH
LIFSYAFEPMAAFGGFAGAAVAETIRWGFARGLYSNEAGLGTAPIAHAAAQTDHPVRQGF
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCHHHHHH
WAVIGIVIDTLIICSTTAFVVLSSGVWTADGAMDDPAALTTIAFREYFGYTGSLLVTISL
HHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHH
IFFVLSTIIVIIFYGSRQAEYLFGLTAGKIMKAVYIVSIVIGAIGGAQMIWNFLDLMLAM
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
ILIPNMIAVLLLSGEVKRLTTEFFTSEKYYKKDIAEEEAS
HHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377 [H]