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The map label for this gene is trmB

Identifier: 229916448

GI number: 229916448

Start: 670461

End: 671108

Strand: Reverse

Name: trmB

Synonym: EAT1b_0718

Alternate gene names: 229916448

Gene position: 671108-670461 (Counterclockwise)

Preceding gene: 229916450

Following gene: 229916447

Centisome position: 22.37

GC content: 44.44

Gene sequence:

>648_bases
ATGCGTTTACGACATAAGCCTTGGGCAAAAGAATATATGGAAGCTCAAGAACATGTATTTATTCAACATCCTGAACAGTT
GAAGGGTAACTGGAGCACTGAATTCGGGAATGACCACCCGCTTTTTATCGAGGTCGGTTCAGGAAAAGGACAATTTATTT
TAGGGATGGCGAAACAACACCCGGATGTGAACTTTATTGCAATCGAGTTGTTTGAGAGTGTAGCCGTCTCCATCGTACAA
AAATTAGTCGAAACCCCGATGCCGAACGTGAGAGTGTTGACGGTCGATGCGAAACGACTCGTCGACTATTTTGAAGCGGG
AGAAGTCGATCGCGTCTACTTAAACTTCTCCGATCCGTGGCCAAAGACACGACATGCGAAACGACGCCTAACGTATAAGA
CGTTTTTAGCGACATATGAGGCGATTCTTCCGAAAGCTGGAGAGATTCATTTCAAGACGGACAACCGTGGATTGTTTGAA
TATTCGCTTCAGAGCATGAGTCAATACGGGATGTTCTTCACAGAAATTTCACTAGATTTACATGTCAATGAACCAGAAGA
TAATATTCGAACAGAGTATGAAGAGCGCTTTAGTGCGCTCGGTCAGCCGATTTACCGAATGGAAGCCGTCTTCCGTCCGA
AAAATTGA

Upstream 100 bases:

>100_bases
TAAACTATGATTTCGTGTATAATCGCAAGAGGACTGCTCGTCCGTAACGTGCATATTCCCATTAAACTTGAACATTTGAT
GAACGAAAGGTAGGATTTTC

Downstream 100 bases:

>100_bases
CACGCTTTTTTCTTATTTTAATAGTATTTTCTTCACACTTTTTTCTTTCTTCTTGGTACAATAGGAATGACGCTAAACTG
GAATAAGAGAAGGGGGAACA

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MRLRHKPWAKEYMEAQEHVFIQHPEQLKGNWSTEFGNDHPLFIEVGSGKGQFILGMAKQHPDVNFIAIELFESVAVSIVQ
KLVETPMPNVRVLTVDAKRLVDYFEAGEVDRVYLNFSDPWPKTRHAKRRLTYKTFLATYEAILPKAGEIHFKTDNRGLFE
YSLQSMSQYGMFFTEISLDLHVNEPEDNIRTEYEERFSALGQPIYRMEAVFRPKN

Sequences:

>Translated_215_residues
MRLRHKPWAKEYMEAQEHVFIQHPEQLKGNWSTEFGNDHPLFIEVGSGKGQFILGMAKQHPDVNFIAIELFESVAVSIVQ
KLVETPMPNVRVLTVDAKRLVDYFEAGEVDRVYLNFSDPWPKTRHAKRRLTYKTFLATYEAILPKAGEIHFKTDNRGLFE
YSLQSMSQYGMFFTEISLDLHVNEPEDNIRTEYEERFSALGQPIYRMEAVFRPKN
>Mature_215_residues
MRLRHKPWAKEYMEAQEHVFIQHPEQLKGNWSTEFGNDHPLFIEVGSGKGQFILGMAKQHPDVNFIAIELFESVAVSIVQ
KLVETPMPNVRVLTVDAKRLVDYFEAGEVDRVYLNFSDPWPKTRHAKRRLTYKTFLATYEAILPKAGEIHFKTDNRGLFE
YSLQSMSQYGMFFTEISLDLHVNEPEDNIRTEYEERFSALGQPIYRMEAVFRPKN

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=174, Percent_Identity=28.1609195402299, Blast_Score=78, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_EXISA (C4L4Q4)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002885094.1
- ProteinModelPortal:   C4L4Q4
- GeneID:   7871490
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_0718
- OMA:   LRNKPWA
- ProtClustDB:   PRK00121
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 25147; Mature: 25147

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: BINDING 44-44 BINDING 69-69 BINDING 96-96 BINDING 118-118 BINDING 122-122 BINDING 154-154

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLRHKPWAKEYMEAQEHVFIQHPEQLKGNWSTEFGNDHPLFIEVGSGKGQFILGMAKQH
CCCCCCCHHHHHHHHHHCEEEECCHHHCCCCCCCCCCCCCEEEEECCCCCCEEEEECCCC
PDVNFIAIELFESVAVSIVQKLVETPMPNVRVLTVDAKRLVDYFEAGEVDRVYLNFSDPW
CCCCEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHCCCEEEEEEECCCCC
PKTRHAKRRLTYKTFLATYEAILPKAGEIHFKTDNRGLFEYSLQSMSQYGMFFTEISLDL
CCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCEEEEEEEEEE
HVNEPEDNIRTEYEERFSALGQPIYRMEAVFRPKN
EECCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCC
>Mature Secondary Structure
MRLRHKPWAKEYMEAQEHVFIQHPEQLKGNWSTEFGNDHPLFIEVGSGKGQFILGMAKQH
CCCCCCCHHHHHHHHHHCEEEECCHHHCCCCCCCCCCCCCEEEEECCCCCCEEEEECCCC
PDVNFIAIELFESVAVSIVQKLVETPMPNVRVLTVDAKRLVDYFEAGEVDRVYLNFSDPW
CCCCEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHCCCEEEEEEECCCCC
PKTRHAKRRLTYKTFLATYEAILPKAGEIHFKTDNRGLFEYSLQSMSQYGMFFTEISLDL
CCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCEEEEEEEEEE
HVNEPEDNIRTEYEERFSALGQPIYRMEAVFRPKN
EECCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA