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The map label for this gene is ytpR [H]

Identifier: 229916437

GI number: 229916437

Start: 662811

End: 663425

Strand: Reverse

Name: ytpR [H]

Synonym: EAT1b_0707

Alternate gene names: 229916437

Gene position: 663425-662811 (Counterclockwise)

Preceding gene: 229916438

Following gene: 229916436

Centisome position: 22.11

GC content: 46.18

Gene sequence:

>615_bases
ATGAATGTGTTTTATAACCGAGAAGGAATCGGTGATGTACTCATGGTGTTGTTAAAAGAAGGAGAACGACATCATCAATC
TGTCGAGCGGACGGGAGACGTGGCAATCGTCAAGTCGGCAGACGGAGAAGTGAATGGGTACAACCTGTTTCAAGCAAGTC
AACATGTTGATGTGACAGGATGGAATGGTCCTGTTGAGTTGACAGTTGAATTAGTGGAACAGTTGCAATCTATCTTGAAT
GATAACGGGATTGAAGAGGACTTTTCATGGGTTGACGTGACGCCGAAGTTTGTCGTCGGGTATGTTGAAAGCGCAGTAAA
ACACCCGGACGCTGACAAACTGTCAATCTGTCAAGTTCAAATCGACCAAGGGACTGTTCAAATAGTATGTGGCGCACCGA
ATGTGGCAACCGGGCAAAAAGTCGTTGTCGCAAAACCGGGTGCGGTGATGCCGACTGGTTTGATTATCCGTCCGTCTGCA
CTCCGTGGTGAAAGTTCACACGGGATGATTTGTTCTGCCCGTGAATTAAACCTTCCGAACGCCCCGCAAGAAAAAGGGAT
TTTAGTATTAGATGACGAATATGAAGTAGGTACAGCATTTAAAATCGGTCGCTAA

Upstream 100 bases:

>100_bases
ACCGATTTTCATTGTCGGAAAAAAGACCCCGCCATCGAACACATAATCTGATAAAATAAGTGAGATGAGTTTGAAAGAAG
GGACGTAGGAACGTATGCAA

Downstream 100 bases:

>100_bases
ATTCGACCGATTTTTTGTTAAATATGGATTGAGTGAAAAAGCGAGGAATCAAGTATGGATGCATATGAACGTTCACTAAA
GCAACAAGCAGAGCGAATCA

Product: t-RNA-binding domain protein

Products: AMP; diphosphate; L-phenylalanyl-tRNAPhe [C]

Alternate protein names: Partial phenylalanyl-tRNA synthetase; Partial PheRS; pFRS [H]

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MNVFYNREGIGDVLMVLLKEGERHHQSVERTGDVAIVKSADGEVNGYNLFQASQHVDVTGWNGPVELTVELVEQLQSILN
DNGIEEDFSWVDVTPKFVVGYVESAVKHPDADKLSICQVQIDQGTVQIVCGAPNVATGQKVVVAKPGAVMPTGLIIRPSA
LRGESSHGMICSARELNLPNAPQEKGILVLDDEYEVGTAFKIGR

Sequences:

>Translated_204_residues
MNVFYNREGIGDVLMVLLKEGERHHQSVERTGDVAIVKSADGEVNGYNLFQASQHVDVTGWNGPVELTVELVEQLQSILN
DNGIEEDFSWVDVTPKFVVGYVESAVKHPDADKLSICQVQIDQGTVQIVCGAPNVATGQKVVVAKPGAVMPTGLIIRPSA
LRGESSHGMICSARELNLPNAPQEKGILVLDDEYEVGTAFKIGR
>Mature_204_residues
MNVFYNREGIGDVLMVLLKEGERHHQSVERTGDVAIVKSADGEVNGYNLFQASQHVDVTGWNGPVELTVELVEQLQSILN
DNGIEEDFSWVDVTPKFVVGYVESAVKHPDADKLSICQVQIDQGTVQIVCGAPNVATGQKVVVAKPGAVMPTGLIIRPSA
LRGESSHGMICSARELNLPNAPQEKGILVLDDEYEVGTAFKIGR

Specific function: Unknown

COG id: COG0073

COG function: function code R; EMAP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788006, Length=96, Percent_Identity=40.625, Blast_Score=71, Evalue=5e-14,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR002547 [H]

Pfam domain/function: PF01588 tRNA_bind [H]

EC number: 6.1.1.20 [C]

Molecular weight: Translated: 22080; Mature: 22080

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVFYNREGIGDVLMVLLKEGERHHQSVERTGDVAIVKSADGEVNGYNLFQASQHVDVTG
CCEEECCCCHHHHHHHHHHCCHHHHHHHHHCCCEEEEECCCCCCCCEEEEECCCEEEEEC
WNGPVELTVELVEQLQSILNDNGIEEDFSWVDVTPKFVVGYVESAVKHPDADKLSICQVQ
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHHHCCCCCCCEEEEEEE
IDQGTVQIVCGAPNVATGQKVVVAKPGAVMPTGLIIRPSALRGESSHGMICSARELNLPN
ECCCEEEEEECCCCCCCCCEEEEECCCCCCCCCEEEECHHCCCCCCCCEEEEHEECCCCC
APQEKGILVLDDEYEVGTAFKIGR
CCCCCCEEEEECCCCCCCEEEECC
>Mature Secondary Structure
MNVFYNREGIGDVLMVLLKEGERHHQSVERTGDVAIVKSADGEVNGYNLFQASQHVDVTG
CCEEECCCCHHHHHHHHHHCCHHHHHHHHHCCCEEEEECCCCCCCCEEEEECCCEEEEEC
WNGPVELTVELVEQLQSILNDNGIEEDFSWVDVTPKFVVGYVESAVKHPDADKLSICQVQ
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHHHCCCCCCCEEEEEEE
IDQGTVQIVCGAPNVATGQKVVVAKPGAVMPTGLIIRPSALRGESSHGMICSARELNLPN
ECCCEEEEEECCCCCCCCCEEEEECCCCCCCCCEEEECHHCCCCCCCCEEEEHEECCCCC
APQEKGILVLDDEYEVGTAFKIGR
CCCCCCEEEEECCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Mg2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.125 {2'-deoxyadenosine} 0.009 {ATP}} 0.2 {2-chloroadenosine} 0.11 {2-chloroadenosine} [C]

Substrates: ATP; @PHEN01.txt*phenylalanine!; tRNAPhe [C]

Specific reaction: ATP + @PHEN01.txt*phenylalanine! + tRNAPhe = AMP + diphosphate + L-phenylalanyl-tRNAPhe [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 3'-Deoxyadenosine 5'-triphosphate; 3'-Methyl adenosine 5'-triphosphate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]