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The map label for this gene is dnaB [H]

Identifier: 229916384

GI number: 229916384

Start: 605571

End: 606923

Strand: Reverse

Name: dnaB [H]

Synonym: EAT1b_0654

Alternate gene names: 229916384

Gene position: 606923-605571 (Counterclockwise)

Preceding gene: 229916385

Following gene: 229916383

Centisome position: 20.23

GC content: 46.56

Gene sequence:

>1353_bases
ATGGAGAAAGAACGTACAATCTTTGGGACGGACGAATTGTTAATCATGAGTACCGGTCTTGTCGACCGTGAATCGTACCA
GTCCCTTTACCACTTATATCAACCTGTCATCGGAGTGAAGGCGCTCGCACTCTATCAAACGTTATGGAGCGAACTGAAAC
CGGGTAAAATGAAATCGAACTATATGTCACATAGTGCGTTATGTGAGATGTTGGAGTTCTCTATCAATGAATTAATGGAC
AGTCTGTTCCGTTTAGAAGCGATTGGGTTAGTGCGCACTTATAAAACAAAAGACGCTCGATTGACACAATACGTCTATCA
GTTACGCGTCCCGCTTGCACCGCACACGTTTTTGAATGACGGGCTGTTGTCGAGTTTCCTCTTCTATAAAGTCGGGGAAG
TACGATTCAAACAATTGCAGGGGCGCTTTACGATTGATCCGTTGCCAGCAGGCATCGTCGAGGTGACAAAGGACTTCAAT
GAAGTATTTGATGTCAAAGGAGTGAACCATCGTGCGTTCACACAAAAAAATTATGAAAAGCCGGACCGGGCTAAGATTGA
GATCAACTATTCGTTCGATATGGAAATTGTGAAAAAACTGACGAATTTTCCAGTTGGATTTTTCACGAAAAAATTAGACG
AAACGATCACAAAACTGGCCTACGTGTCTCAACTTGATGAAGAGACGATGGCTGAGATGTTAAAAGAATATTTCGTCCAT
GAGGCGTATCTGCCACTCAGTGAGCAAGAGAAGCGGGACGGGTGTCGTCAAATGGTCTTACAATTGAAAAAGAAACAGAC
GCGCCATCGGAAAGAGAAGACCGTCGAACTTGAACAGACGAAACTTGGAGACTTGCATGACCCTGAGGTGATGGAGTCCG
CACATCCGCATCAATATGTCTCGACGCTTCGGAAGACCCCGCAACTTTCAAAGAGTGATGAACAGTTGGTCATTGAACTC
GTCGACACACTCGGTCTCGCGCCAGGGGTCATCAATGCGCTCTTTTATTACTGTATCGAAGTGAAGAAGCAGACGCGGCT
GAGCGAGAACTATGTCTTGCGCATTGCGACAAGTTGGAAAACAGCAGGCTATACGAACGCAAAAGAAGCGCTAGAGCGTG
AAGAATCTCAAGATGCGCTCATCGAGAAGAAACAACAAAAACGCGAGAGCGTGCAACGCACCACGGTCGGGTCACGACGG
AAACCCCAACAAGGGGATATGCCGAAGTGGCTCGAGGATGAGGCGAAACAACAGCGCTCATACGACCAAGCGAGGAAAAA
GGAACTCGAGTCGTCCGTTCCGGATGACGCGGAACTTGTGAAACTATTAAATGAATTGAAAGAGAAGGGGTGA

Upstream 100 bases:

>100_bases
ATCACGGTGTTCTTTAAAGAACTCGAAGATTTAATGAAGCAAGAAAATTCGACCAACTAAAGGGCGGCCTGCTGGTCGCC
CTTTTTCAAGGGAGAGAGCC

Downstream 100 bases:

>100_bases
GAGGATGGAACGAATCAATCAGTCGCTGTCTCAGTTGATGAACCGAAAAGAAGTTCGTGAGGCGTACGAGTCGATTCGCA
GACGTACACTGAATCACCCG

Product: replication initiation and membrane attachment family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MEKERTIFGTDELLIMSTGLVDRESYQSLYHLYQPVIGVKALALYQTLWSELKPGKMKSNYMSHSALCEMLEFSINELMD
SLFRLEAIGLVRTYKTKDARLTQYVYQLRVPLAPHTFLNDGLLSSFLFYKVGEVRFKQLQGRFTIDPLPAGIVEVTKDFN
EVFDVKGVNHRAFTQKNYEKPDRAKIEINYSFDMEIVKKLTNFPVGFFTKKLDETITKLAYVSQLDEETMAEMLKEYFVH
EAYLPLSEQEKRDGCRQMVLQLKKKQTRHRKEKTVELEQTKLGDLHDPEVMESAHPHQYVSTLRKTPQLSKSDEQLVIEL
VDTLGLAPGVINALFYYCIEVKKQTRLSENYVLRIATSWKTAGYTNAKEALEREESQDALIEKKQQKRESVQRTTVGSRR
KPQQGDMPKWLEDEAKQQRSYDQARKKELESSVPDDAELVKLLNELKEKG

Sequences:

>Translated_450_residues
MEKERTIFGTDELLIMSTGLVDRESYQSLYHLYQPVIGVKALALYQTLWSELKPGKMKSNYMSHSALCEMLEFSINELMD
SLFRLEAIGLVRTYKTKDARLTQYVYQLRVPLAPHTFLNDGLLSSFLFYKVGEVRFKQLQGRFTIDPLPAGIVEVTKDFN
EVFDVKGVNHRAFTQKNYEKPDRAKIEINYSFDMEIVKKLTNFPVGFFTKKLDETITKLAYVSQLDEETMAEMLKEYFVH
EAYLPLSEQEKRDGCRQMVLQLKKKQTRHRKEKTVELEQTKLGDLHDPEVMESAHPHQYVSTLRKTPQLSKSDEQLVIEL
VDTLGLAPGVINALFYYCIEVKKQTRLSENYVLRIATSWKTAGYTNAKEALEREESQDALIEKKQQKRESVQRTTVGSRR
KPQQGDMPKWLEDEAKQQRSYDQARKKELESSVPDDAELVKLLNELKEKG
>Mature_450_residues
MEKERTIFGTDELLIMSTGLVDRESYQSLYHLYQPVIGVKALALYQTLWSELKPGKMKSNYMSHSALCEMLEFSINELMD
SLFRLEAIGLVRTYKTKDARLTQYVYQLRVPLAPHTFLNDGLLSSFLFYKVGEVRFKQLQGRFTIDPLPAGIVEVTKDFN
EVFDVKGVNHRAFTQKNYEKPDRAKIEINYSFDMEIVKKLTNFPVGFFTKKLDETITKLAYVSQLDEETMAEMLKEYFVH
EAYLPLSEQEKRDGCRQMVLQLKKKQTRHRKEKTVELEQTKLGDLHDPEVMESAHPHQYVSTLRKTPQLSKSDEQLVIEL
VDTLGLAPGVINALFYYCIEVKKQTRLSENYVLRIATSWKTAGYTNAKEALEREESQDALIEKKQQKRESVQRTTVGSRR
KPQQGDMPKWLEDEAKQQRSYDQARKKELESSVPDDAELVKLLNELKEKG

Specific function: Probable component of primosome involved in the initiation of DNA replication. It is essential for both replication initiation and membrane attachment of the origin region of the chromosome and plasmid pUB110 [H]

COG id: COG3611

COG function: function code L; Replication initiation/membrane attachment protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006343 [H]

Pfam domain/function: PF07261 DnaB_2 [H]

EC number: NA

Molecular weight: Translated: 52285; Mature: 52285

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKERTIFGTDELLIMSTGLVDRESYQSLYHLYQPVIGVKALALYQTLWSELKPGKMKSN
CCCCCCEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
YMSHSALCEMLEFSINELMDSLFRLEAIGLVRTYKTKDARLTQYVYQLRVPLAPHTFLND
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHH
GLLSSFLFYKVGEVRFKQLQGRFTIDPLPAGIVEVTKDFNEVFDVKGVNHRAFTQKNYEK
HHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCC
PDRAKIEINYSFDMEIVKKLTNFPVGFFTKKLDETITKLAYVSQLDEETMAEMLKEYFVH
CCCEEEEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
EAYLPLSEQEKRDGCRQMVLQLKKKQTRHRKEKTVELEQTKLGDLHDPEVMESAHPHQYV
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHH
STLRKTPQLSKSDEQLVIELVDTLGLAPGVINALFYYCIEVKKQTRLSENYVLRIATSWK
HHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
TAGYTNAKEALEREESQDALIEKKQQKRESVQRTTVGSRRKPQQGDMPKWLEDEAKQQRS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
YDQARKKELESSVPDDAELVKLLNELKEKG
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEKERTIFGTDELLIMSTGLVDRESYQSLYHLYQPVIGVKALALYQTLWSELKPGKMKSN
CCCCCCEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
YMSHSALCEMLEFSINELMDSLFRLEAIGLVRTYKTKDARLTQYVYQLRVPLAPHTFLND
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHH
GLLSSFLFYKVGEVRFKQLQGRFTIDPLPAGIVEVTKDFNEVFDVKGVNHRAFTQKNYEK
HHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCC
PDRAKIEINYSFDMEIVKKLTNFPVGFFTKKLDETITKLAYVSQLDEETMAEMLKEYFVH
CCCEEEEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
EAYLPLSEQEKRDGCRQMVLQLKKKQTRHRKEKTVELEQTKLGDLHDPEVMESAHPHQYV
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHH
STLRKTPQLSKSDEQLVIELVDTLGLAPGVINALFYYCIEVKKQTRLSENYVLRIATSWK
HHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
TAGYTNAKEALEREESQDALIEKKQQKRESVQRTTVGSRRKPQQGDMPKWLEDEAKQQRS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
YDQARKKELESSVPDDAELVKLLNELKEKG
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3027697; 3027671; 8969504; 9387221; 9384377 [H]