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The map label for this gene is thrS

Identifier: 229916382

GI number: 229916382

Start: 602410

End: 604353

Strand: Reverse

Name: thrS

Synonym: EAT1b_0652

Alternate gene names: 229916382

Gene position: 604353-602410 (Counterclockwise)

Preceding gene: 229916383

Following gene: 229916381

Centisome position: 20.15

GC content: 49.07

Gene sequence:

>1944_bases
ATGTACATGATTCAATTAACATTTCCAGATGGCGCGGTAAAAGAGTTCGAAGCCGGCGTCACAGCAGAAGACGTCGCGGG
CTCGATCAGTCCGGGACTCCGTAAAAAAGCAATCGCAGCAAAACTAGACGGAGAACTCATCGACTACCGTCGTCCAATCG
AACATGACGGCAAAATCGAGCTCGTGATGCCGGACTCGGAAGACGGTCTTGATTTGATGCGTCACTCGTCGGCTCACTTG
ATGGCTCAAGCAATCAAACGGTTATACGGAGAAGACAACAACATCTATCTCGGAATTGGACCGACGATTGAGAACGGATT
CTATTACGATATCGAGATGGATCGTCGCATCAATGAAGAAGACCTTCCTGAAATCGAAAAGATGATGAAGCGCATCGTCG
ATGAGAACTTGGAAATCACACGCGAAGTCGTTTCTCGTGACGAGGCGCTCGCTCGCTATAAAGAGTTGGGAGACCCGCTC
AAGATCGAGTTGATCGAGGATATTCCTGCTGATCAAACACTGACAATCTACCATCAAGGTGAGTTCTTTGACCTTTGCCG
TGGACCACACGTCCCGTCAACATCGAAGTTGAAAATTTTCAAATTGATGAGCGTGGCTGGTGCCTACTGGCGTGGGGACT
CTGACAATAAGATGTTACAGCGTATTTATGGAACAGCGTGGGCGACGAAAGAACAACTCGCTGAACACTTGCGTCTTCTT
GAAGAGGCGAAAGAACGCGATCATCGTAAACTTGGGAAAGAACTCGACCTCTTCTTCGTCTCACAAGAAGTCGGTCAGGG
GCTTCCGATGTGGCTTCCAAAAGGGGCTTCGATTCGTCGTACGGTCGAGCGCTACATCGTTGATAAAGAGCTCGAACTCG
GATATCAACATGTCTACACACCGGTTCTCGGTTCAGTCGACTTGTACAAGACATCTGGACACTGGGATCACTACCAAGAT
GACATGTTCCCGAAAATGGAGATGGACAACGAAGAACTCGTCCTTCGTCCGATGAACTGCCCACACCATATGACAATCTA
CAAACATGAGCCACGTTCGTACCGTGAACTTCCACTTCGAATCGCTGAGCTTGGTGGGATGCACCGTTATGAGATGTCAG
GGGCGCTCACAGGTCTTCAACGTGTTCGCTACATGGTCTTGAATGATGGACATACGTTCGTTACACCAGAACAGATGAAA
CAAGAATTCAAAGACATCGTTCATTTGATTCAAGAAGTATACGCTGACTTCGGTATCAAAGATTATCGTTTCCGTCTCTC
ATATCGCGATCCTGCTGATAAAGAGAAGTACTTTGATAACGATGCGATTTGGGAAATGGCACAGAGTCAGTTGAAGGAAA
CGATGGATGAGCTCGAGCTTCCGTACTTTGAGGCGGAAGGGGAAGCGGCATTCTACGGACCGAAACTTGACGTGCAAGTA
CGGACGGCGCTCGGGAAAGAAGAGACGCTCTCGACTGTCCAACTCGACTTCTTGCTTCCGGAACGTTTCGATTTGACATA
CACTGGGCCAGACGGTAAGGACCACCGTCCAATCGTGTTGCACCGTGGTGTCGTCTCGACGATGGAACGGTTTGTCGCTT
ACTTGATTGAAGAGTATAAAGGCGCGTTCCCGACATGGCTCGCACCGGTCCAAGTCAAGTTGATCCCGGTTTCACACGTA
CACGACGAATACGTCGCGGAAGTGAAAGCAGAACTCGTCAAACGTGGCGTCCGTGTCGAAACGGATCTCCGTGACGAGAA
ACTAGGTTATAAGATTCGCGAAGCACAAATGAAGAAGATTCCGATGACGCTCGTTCTTGGGGATAAAGAACGGGATGAGC
GTGCCGTCAATATTCGCCGCTACGGTCAGCAGGAGCAAGTTTCTGCTTCACTTGATGAATTTATCGCATCGCTCACAGAT
GAGATTGCGAACCGTTCACGTTAA

Upstream 100 bases:

>100_bases
CAAGCAGATTGCTTGGGAACGAGGGTGGTACCACGAGAATCAAAGCTCGTCCCTTTTTGAGGGATGGGCTTTTTTTATAT
TCATTTTTGAGAGGGGACAA

Downstream 100 bases:

>100_bases
GTGAGGGACCACCTTTCCAAACAGAAAGGTGGTTTTTTTCGGTCTATAGACGGATGTGTATTTGTTCATCTACACGTATC
GTAAACATAAGGAGTGATAC

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS

Number of amino acids: Translated: 647; Mature: 647

Protein sequence:

>647_residues
MYMIQLTFPDGAVKEFEAGVTAEDVAGSISPGLRKKAIAAKLDGELIDYRRPIEHDGKIELVMPDSEDGLDLMRHSSAHL
MAQAIKRLYGEDNNIYLGIGPTIENGFYYDIEMDRRINEEDLPEIEKMMKRIVDENLEITREVVSRDEALARYKELGDPL
KIELIEDIPADQTLTIYHQGEFFDLCRGPHVPSTSKLKIFKLMSVAGAYWRGDSDNKMLQRIYGTAWATKEQLAEHLRLL
EEAKERDHRKLGKELDLFFVSQEVGQGLPMWLPKGASIRRTVERYIVDKELELGYQHVYTPVLGSVDLYKTSGHWDHYQD
DMFPKMEMDNEELVLRPMNCPHHMTIYKHEPRSYRELPLRIAELGGMHRYEMSGALTGLQRVRYMVLNDGHTFVTPEQMK
QEFKDIVHLIQEVYADFGIKDYRFRLSYRDPADKEKYFDNDAIWEMAQSQLKETMDELELPYFEAEGEAAFYGPKLDVQV
RTALGKEETLSTVQLDFLLPERFDLTYTGPDGKDHRPIVLHRGVVSTMERFVAYLIEEYKGAFPTWLAPVQVKLIPVSHV
HDEYVAEVKAELVKRGVRVETDLRDEKLGYKIREAQMKKIPMTLVLGDKERDERAVNIRRYGQQEQVSASLDEFIASLTD
EIANRSR

Sequences:

>Translated_647_residues
MYMIQLTFPDGAVKEFEAGVTAEDVAGSISPGLRKKAIAAKLDGELIDYRRPIEHDGKIELVMPDSEDGLDLMRHSSAHL
MAQAIKRLYGEDNNIYLGIGPTIENGFYYDIEMDRRINEEDLPEIEKMMKRIVDENLEITREVVSRDEALARYKELGDPL
KIELIEDIPADQTLTIYHQGEFFDLCRGPHVPSTSKLKIFKLMSVAGAYWRGDSDNKMLQRIYGTAWATKEQLAEHLRLL
EEAKERDHRKLGKELDLFFVSQEVGQGLPMWLPKGASIRRTVERYIVDKELELGYQHVYTPVLGSVDLYKTSGHWDHYQD
DMFPKMEMDNEELVLRPMNCPHHMTIYKHEPRSYRELPLRIAELGGMHRYEMSGALTGLQRVRYMVLNDGHTFVTPEQMK
QEFKDIVHLIQEVYADFGIKDYRFRLSYRDPADKEKYFDNDAIWEMAQSQLKETMDELELPYFEAEGEAAFYGPKLDVQV
RTALGKEETLSTVQLDFLLPERFDLTYTGPDGKDHRPIVLHRGVVSTMERFVAYLIEEYKGAFPTWLAPVQVKLIPVSHV
HDEYVAEVKAELVKRGVRVETDLRDEKLGYKIREAQMKKIPMTLVLGDKERDERAVNIRRYGQQEQVSASLDEFIASLTD
EIANRSR
>Mature_647_residues
MYMIQLTFPDGAVKEFEAGVTAEDVAGSISPGLRKKAIAAKLDGELIDYRRPIEHDGKIELVMPDSEDGLDLMRHSSAHL
MAQAIKRLYGEDNNIYLGIGPTIENGFYYDIEMDRRINEEDLPEIEKMMKRIVDENLEITREVVSRDEALARYKELGDPL
KIELIEDIPADQTLTIYHQGEFFDLCRGPHVPSTSKLKIFKLMSVAGAYWRGDSDNKMLQRIYGTAWATKEQLAEHLRLL
EEAKERDHRKLGKELDLFFVSQEVGQGLPMWLPKGASIRRTVERYIVDKELELGYQHVYTPVLGSVDLYKTSGHWDHYQD
DMFPKMEMDNEELVLRPMNCPHHMTIYKHEPRSYRELPLRIAELGGMHRYEMSGALTGLQRVRYMVLNDGHTFVTPEQMK
QEFKDIVHLIQEVYADFGIKDYRFRLSYRDPADKEKYFDNDAIWEMAQSQLKETMDELELPYFEAEGEAAFYGPKLDVQV
RTALGKEETLSTVQLDFLLPERFDLTYTGPDGKDHRPIVLHRGVVSTMERFVAYLIEEYKGAFPTWLAPVQVKLIPVSHV
HDEYVAEVKAELVKRGVRVETDLRDEKLGYKIREAQMKKIPMTLVLGDKERDERAVNIRRYGQQEQVSASLDEFIASLTD
EIANRSR

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI38202255, Length=648, Percent_Identity=37.6543209876543, Blast_Score=450, Evalue=1e-126,
Organism=Homo sapiens, GI38570111, Length=621, Percent_Identity=37.1980676328502, Blast_Score=443, Evalue=1e-124,
Organism=Homo sapiens, GI20070344, Length=636, Percent_Identity=35.5345911949685, Blast_Score=389, Evalue=1e-108,
Organism=Escherichia coli, GI1788013, Length=645, Percent_Identity=41.5503875968992, Blast_Score=537, Evalue=1e-154,
Organism=Escherichia coli, GI1786392, Length=163, Percent_Identity=25.1533742331288, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71984184, Length=638, Percent_Identity=36.2068965517241, Blast_Score=435, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI71984192, Length=639, Percent_Identity=36.4632237871675, Blast_Score=435, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6322112, Length=661, Percent_Identity=34.0393343419062, Blast_Score=413, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6322654, Length=390, Percent_Identity=35.3846153846154, Blast_Score=243, Evalue=9e-65,
Organism=Drosophila melanogaster, GI24583842, Length=638, Percent_Identity=38.2445141065831, Blast_Score=454, Evalue=1e-128,
Organism=Drosophila melanogaster, GI28574141, Length=638, Percent_Identity=38.2445141065831, Blast_Score=454, Evalue=1e-128,
Organism=Drosophila melanogaster, GI24583839, Length=638, Percent_Identity=38.2445141065831, Blast_Score=454, Evalue=1e-128,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYT_EXISA (C4L461)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002885028.1
- ProteinModelPortal:   C4L461
- GeneID:   7871424
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_0652
- OMA:   GRKWQLG
- ProtClustDB:   PRK00413
- GO:   GO:0005737
- HAMAP:   MF_00184_B
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.10.20.30
- PRINTS:   PR01047
- SMART:   SM00863
- TIGRFAMs:   TIGR00418

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD; SSF52954 Anticodon_bd; SSF81271 TGS-like; SSF55186 Thr/Ala-tRNA-synth_IIc_edit

EC number: =6.1.1.3

Molecular weight: Translated: 74916; Mature: 74916

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYMIQLTFPDGAVKEFEAGVTAEDVAGSISPGLRKKAIAAKLDGELIDYRRPIEHDGKIE
CEEEEEECCCCHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHCCCCHHHCCCCCCCCCEEE
LVMPDSEDGLDLMRHSSAHLMAQAIKRLYGEDNNIYLGIGPTIENGFYYDIEMDRRINEE
EEECCCCCCHHHHHCCHHHHHHHHHHHHHCCCCCEEEEECCEECCCEEEEEEECCCCCCC
DLPEIEKMMKRIVDENLEITREVVSRDEALARYKELGDPLKIELIEDIPADQTLTIYHQG
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEECC
EFFDLCRGPHVPSTSKLKIFKLMSVAGAYWRGDSDNKMLQRIYGTAWATKEQLAEHLRLL
CCHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHH
EEAKERDHRKLGKELDLFFVSQEVGQGLPMWLPKGASIRRTVERYIVDKELELGYQHVYT
HHHHHHHHHHHCCHHHEEEEEHHHCCCCCCCCCCCCHHHHHHHHHHHCHHHHHCHHHHHH
PVLGSVDLYKTSGHWDHYQDDMFPKMEMDNEELVLRPMNCPHHMTIYKHEPRSYRELPLR
HHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEECCCCCHHHHHHH
IAELGGMHRYEMSGALTGLQRVRYMVLNDGHTFVTPEQMKQEFKDIVHLIQEVYADFGIK
HHHHCCCCEEHHHHHHHHHHHHEEEEEECCCCEECHHHHHHHHHHHHHHHHHHHHHCCCE
DYRFRLSYRDPADKEKYFDNDAIWEMAQSQLKETMDELELPYFEAEGEAAFYGPKLDVQV
EEEEEEECCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCEEEECCCCCEEE
RTALGKEETLSTVQLDFLLPERFDLTYTGPDGKDHRPIVLHRGVVSTMERFVAYLIEEYK
HHHCCCHHHHHEEEEEEECCCCCEEEECCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHC
GAFPTWLAPVQVKLIPVSHVHDEYVAEVKAELVKRGVRVETDLRDEKLGYKIREAQMKKI
CCCCCEECCEEEEEEEHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHCCHHHHHHHHHC
PMTLVLGDKERDERAVNIRRYGQQEQVSASLDEFIASLTDEIANRSR
CEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MYMIQLTFPDGAVKEFEAGVTAEDVAGSISPGLRKKAIAAKLDGELIDYRRPIEHDGKIE
CEEEEEECCCCHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHCCCCHHHCCCCCCCCCEEE
LVMPDSEDGLDLMRHSSAHLMAQAIKRLYGEDNNIYLGIGPTIENGFYYDIEMDRRINEE
EEECCCCCCHHHHHCCHHHHHHHHHHHHHCCCCCEEEEECCEECCCEEEEEEECCCCCCC
DLPEIEKMMKRIVDENLEITREVVSRDEALARYKELGDPLKIELIEDIPADQTLTIYHQG
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEECC
EFFDLCRGPHVPSTSKLKIFKLMSVAGAYWRGDSDNKMLQRIYGTAWATKEQLAEHLRLL
CCHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHH
EEAKERDHRKLGKELDLFFVSQEVGQGLPMWLPKGASIRRTVERYIVDKELELGYQHVYT
HHHHHHHHHHHCCHHHEEEEEHHHCCCCCCCCCCCCHHHHHHHHHHHCHHHHHCHHHHHH
PVLGSVDLYKTSGHWDHYQDDMFPKMEMDNEELVLRPMNCPHHMTIYKHEPRSYRELPLR
HHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEECCCCCHHHHHHH
IAELGGMHRYEMSGALTGLQRVRYMVLNDGHTFVTPEQMKQEFKDIVHLIQEVYADFGIK
HHHHCCCCEEHHHHHHHHHHHHEEEEEECCCCEECHHHHHHHHHHHHHHHHHHHHHCCCE
DYRFRLSYRDPADKEKYFDNDAIWEMAQSQLKETMDELELPYFEAEGEAAFYGPKLDVQV
EEEEEEECCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCEEEECCCCCEEE
RTALGKEETLSTVQLDFLLPERFDLTYTGPDGKDHRPIVLHRGVVSTMERFVAYLIEEYK
HHHCCCHHHHHEEEEEEECCCCCEEEECCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHC
GAFPTWLAPVQVKLIPVSHVHDEYVAEVKAELVKRGVRVETDLRDEKLGYKIREAQMKKI
CCCCCEECCEEEEEEEHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHCCHHHHHHHHHC
PMTLVLGDKERDERAVNIRRYGQQEQVSASLDEFIASLTDEIANRSR
CEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA