Click here to switch to the map view.

The map label for this gene is ysgA [H]

Identifier: 229916372

GI number: 229916372

Start: 596440

End: 597186

Strand: Reverse

Name: ysgA [H]

Synonym: EAT1b_0642

Alternate gene names: 229916372

Gene position: 597186-596440 (Counterclockwise)

Preceding gene: 229916373

Following gene: 229916371

Centisome position: 19.91

GC content: 50.33

Gene sequence:

>747_bases
ATGACGAAGATTACGTCTACGAAGAGCGAGACGATTAAACGATTAAAACGATTGATGACGAAAAAAGGTCGTCAAGAAAC
GGGTCAATTCCTAGTAGAAGGAGAGCATTTGGTGGACGAGGCCATTCGTGCCGGTCGACTCGTCCAACTCATCATGGCTG
AGTCGTATTCACCAAAAGGAACGTGGCGTCTTGAAGATATGCCAGTCCTCGAGTTATCCGACCACGTTGCGGACCTTCTT
TCCGAAACGGAAAAGACCCAAGGCGTGTTTGCCGTTGTCAAGATGACACCGGTGGAACGAAAAATGAAGAAAGTACTCGT
ACTAGACCGAGTACAGGACCCGGGTAATCTCGGTACGATGATTCGGACAGCAGATGCTGCCGGATTCGATACGGTGCTAC
TCGGGAAAGGGACGGTCGATCCGTTCAACGCCAAAGTCGTACGTGCCACACAAGGTTCACTGTTTCACGTGAACGTGACC
TCGGTCGACTTGCTTGAAAAGTTACCTGAGCTGAAAGAGGATGGATTCCACGTCTACGGCACAGCTCTCTCAAAGGCAAC
ATCGTATGAGCAAGTTGCATTCGAAGAAAAAGTGGCCCTCGTCATCGGGAATGAGGCACAAGGGGTTCATGCTGATGTAT
TGGCACTCTGTGACACACGTGTGCATATTCCGGTGCTCGGCGACGTGGAATCGCTCAACGCGGCGGTCGCTGCCGGTATT
TTAATGTATCGCATCGCTTTACAATAA

Upstream 100 bases:

>100_bases
ATCATCCATAAGGATGATTACGAGGCAGCGGTACAACTCGTTGTCGCATTGACGAAACGTTTAGACCACGAAACGGTCCA
ATGGTTGAAAGAGGGATAAT

Downstream 100 bases:

>100_bases
TCTTGTCAGCACGTGAAAAACGTGATAGGTTAAACACATCGATGAATAAGATATGACGTTGAAAGAACATAGTAAGCGAA
TGACCGATTCCGTTCAGGGA

Product: tRNA/rRNA methyltransferase (SpoU)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MTKITSTKSETIKRLKRLMTKKGRQETGQFLVEGEHLVDEAIRAGRLVQLIMAESYSPKGTWRLEDMPVLELSDHVADLL
SETEKTQGVFAVVKMTPVERKMKKVLVLDRVQDPGNLGTMIRTADAAGFDTVLLGKGTVDPFNAKVVRATQGSLFHVNVT
SVDLLEKLPELKEDGFHVYGTALSKATSYEQVAFEEKVALVIGNEAQGVHADVLALCDTRVHIPVLGDVESLNAAVAAGI
LMYRIALQ

Sequences:

>Translated_248_residues
MTKITSTKSETIKRLKRLMTKKGRQETGQFLVEGEHLVDEAIRAGRLVQLIMAESYSPKGTWRLEDMPVLELSDHVADLL
SETEKTQGVFAVVKMTPVERKMKKVLVLDRVQDPGNLGTMIRTADAAGFDTVLLGKGTVDPFNAKVVRATQGSLFHVNVT
SVDLLEKLPELKEDGFHVYGTALSKATSYEQVAFEEKVALVIGNEAQGVHADVLALCDTRVHIPVLGDVESLNAAVAAGI
LMYRIALQ
>Mature_247_residues
TKITSTKSETIKRLKRLMTKKGRQETGQFLVEGEHLVDEAIRAGRLVQLIMAESYSPKGTWRLEDMPVLELSDHVADLLS
ETEKTQGVFAVVKMTPVERKMKKVLVLDRVQDPGNLGTMIRTADAAGFDTVLLGKGTVDPFNAKVVRATQGSLFHVNVTS
VDLLEKLPELKEDGFHVYGTALSKATSYEQVAFEEKVALVIGNEAQGVHADVLALCDTRVHIPVLGDVESLNAAVAAGIL
MYRIALQ

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Homo sapiens, GI8922534, Length=304, Percent_Identity=27.6315789473684, Blast_Score=91, Evalue=9e-19,
Organism=Homo sapiens, GI40068479, Length=213, Percent_Identity=29.1079812206573, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1790623, Length=140, Percent_Identity=34.2857142857143, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1790083, Length=148, Percent_Identity=29.7297297297297, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17555908, Length=197, Percent_Identity=32.48730964467, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24666840, Length=286, Percent_Identity=23.7762237762238, Blast_Score=67, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27157; Mature: 27026

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKITSTKSETIKRLKRLMTKKGRQETGQFLVEGEHLVDEAIRAGRLVQLIMAESYSPKG
CCCCCCCHHHHHHHHHHHHHHCCCHHHCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCCC
TWRLEDMPVLELSDHVADLLSETEKTQGVFAVVKMTPVERKMKKVLVLDRVQDPGNLGTM
CEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHCCCCCCCCCHH
IRTADAAGFDTVLLGKGTVDPFNAKVVRATQGSLFHVNVTSVDLLEKLPELKEDGFHVYG
EEECCCCCCCEEEECCCCCCCCCCEEEEECCCCEEEEEECHHHHHHHHHHHHHCCEEEEE
TALSKATSYEQVAFEEKVALVIGNEAQGVHADVLALCDTRVHIPVLGDVESLNAAVAAGI
HHHHHHCCHHHHHHHCEEEEEECCCCCCCHHHHHHHHCCEEEEEEECCHHHHHHHHHHHH
LMYRIALQ
HHHHHHCC
>Mature Secondary Structure 
TKITSTKSETIKRLKRLMTKKGRQETGQFLVEGEHLVDEAIRAGRLVQLIMAESYSPKG
CCCCCCHHHHHHHHHHHHHHCCCHHHCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCCC
TWRLEDMPVLELSDHVADLLSETEKTQGVFAVVKMTPVERKMKKVLVLDRVQDPGNLGTM
CEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHCCCCCCCCCHH
IRTADAAGFDTVLLGKGTVDPFNAKVVRATQGSLFHVNVTSVDLLEKLPELKEDGFHVYG
EEECCCCCCCEEEECCCCCCCCCCEEEEECCCCEEEEEECHHHHHHHHHHHHHCCEEEEE
TALSKATSYEQVAFEEKVALVIGNEAQGVHADVLALCDTRVHIPVLGDVESLNAAVAAGI
HHHHHHCCHHHHHHHCEEEEEECCCCCCCHHHHHHHHCCEEEEEEECCHHHHHHHHHHHH
LMYRIALQ
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]