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The map label for this gene is nfo [H]

Identifier: 229916312

GI number: 229916312

Start: 531017

End: 531919

Strand: Reverse

Name: nfo [H]

Synonym: EAT1b_0581

Alternate gene names: 229916312

Gene position: 531919-531017 (Counterclockwise)

Preceding gene: 229916313

Following gene: 229916311

Centisome position: 17.73

GC content: 46.29

Gene sequence:

>903_bases
ATGTTAAAAATAGGTTCACACGTTTCGGTATCAGGTAAAAAGATGTTGTTAGCAGGGAGTGAAGAGGCAGTCTCTTACGG
GGCGAATACGATGATGGTGTATACAGGTGCGCCCCAAAACACACGCCGTAAACCCATTGAAGATTTAAATATTGAGGCAG
CACTCAAACATATGGCCGCACATGACATCGAAGAGATTGTTGTTCACGCACCATATATCATCAATCTCGGGAATACGACG
AAACCAGAGACGTTTGAATTGGCCGTCTCTTTCTTAACTCAGGAATTAAAGCGGGCAGAAGCACTCCAAGTGGCTCGTCA
CGTCGTTCTTCACCCGGGCGCTCATGTCGGAGCGGGAGAAGAAATCGGTCTCGCTCGAATCATCGAAGGATTGAATGAAG
TGTTGACGGGTGATGAAAAAGTGAAGATTGCATTGGAGACGATGGCAGGGAAAGGAAGCGAACTTGGTAAGACATTTGAA
GAGCTTGCAACCATCATTGATGGCGTGACGCACAATGATCGACTCTCAGTTTGCTTTGATACGTGTCACGTCCACGATGC
CGGATATGACTTAGTCGGTGATTTAGACGGCGTCATCGAACAATTCGATCGAATCGTCGGACTCGACCGTCTCGGAGTCA
TTCATGTAAATGATTCGAAGAACGTGCGCGGTGCGAAAAAAGACCGTCATGAAAACATCGGCTACGGAGAGATCGGGTTT
GATACATTGAATCGTGTCATTCATCACGAGGCATTTGCGCATCTACCGAAAATCCTAGAGACGCCATATATCCCGATTAG
CGAAAAAACGAAAGTCGCTCCATATAAACAAGAGATTGAAATGTTCCGTACCAATCAATTTGATGCCGAATGGCGTGAAC
GATTCACGCTCGCTCATCAATAA

Upstream 100 bases:

>100_bases
AAGAAAAACAAACGTTAATTTTTTGAGAGAATCGACTTCTTGGAGTCGGTTCTTCTTTTTGTGTAAACTAAAGAGAGAAG
CAAATGAAGGGAAGATGAGG

Downstream 100 bases:

>100_bases
ACGATGAACGTCCTACAAGTCGATCCAAAGCGATATGATGTCTGGAACGCATTCCCGACTTATTTTCTTGAACAAAGTCC
GGTTTATGTAAAGGGGTCCG

Product: apurinic endonuclease Apn1

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MLKIGSHVSVSGKKMLLAGSEEAVSYGANTMMVYTGAPQNTRRKPIEDLNIEAALKHMAAHDIEEIVVHAPYIINLGNTT
KPETFELAVSFLTQELKRAEALQVARHVVLHPGAHVGAGEEIGLARIIEGLNEVLTGDEKVKIALETMAGKGSELGKTFE
ELATIIDGVTHNDRLSVCFDTCHVHDAGYDLVGDLDGVIEQFDRIVGLDRLGVIHVNDSKNVRGAKKDRHENIGYGEIGF
DTLNRVIHHEAFAHLPKILETPYIPISEKTKVAPYKQEIEMFRTNQFDAEWRERFTLAHQ

Sequences:

>Translated_300_residues
MLKIGSHVSVSGKKMLLAGSEEAVSYGANTMMVYTGAPQNTRRKPIEDLNIEAALKHMAAHDIEEIVVHAPYIINLGNTT
KPETFELAVSFLTQELKRAEALQVARHVVLHPGAHVGAGEEIGLARIIEGLNEVLTGDEKVKIALETMAGKGSELGKTFE
ELATIIDGVTHNDRLSVCFDTCHVHDAGYDLVGDLDGVIEQFDRIVGLDRLGVIHVNDSKNVRGAKKDRHENIGYGEIGF
DTLNRVIHHEAFAHLPKILETPYIPISEKTKVAPYKQEIEMFRTNQFDAEWRERFTLAHQ
>Mature_300_residues
MLKIGSHVSVSGKKMLLAGSEEAVSYGANTMMVYTGAPQNTRRKPIEDLNIEAALKHMAAHDIEEIVVHAPYIINLGNTT
KPETFELAVSFLTQELKRAEALQVARHVVLHPGAHVGAGEEIGLARIIEGLNEVLTGDEKVKIALETMAGKGSELGKTFE
ELATIIDGVTHNDRLSVCFDTCHVHDAGYDLVGDLDGVIEQFDRIVGLDRLGVIHVNDSKNVRGAKKDRHENIGYGEIGF
DTLNRVIHHEAFAHLPKILETPYIPISEKTKVAPYKQEIEMFRTNQFDAEWRERFTLAHQ

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788483, Length=266, Percent_Identity=35.3383458646617, Blast_Score=144, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI17531193, Length=270, Percent_Identity=35.1851851851852, Blast_Score=142, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6322735, Length=266, Percent_Identity=29.6992481203008, Blast_Score=136, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: =3.1.21.2 [H]

Molecular weight: Translated: 33246; Mature: 33246

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00729 AP_NUCLEASE_F2_1 ; PS00730 AP_NUCLEASE_F2_2 ; PS00731 AP_NUCLEASE_F2_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKIGSHVSVSGKKMLLAGSEEAVSYGANTMMVYTGAPQNTRRKPIEDLNIEAALKHMAA
CCCCCCEEEECCCEEEEECCHHHHHCCCCEEEEEECCCCCCCCCCCHHCCHHHHHHHHHH
HDIEEIVVHAPYIINLGNTTKPETFELAVSFLTQELKRAEALQVARHVVLHPGAHVGAGE
HHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
EIGLARIIEGLNEVLTGDEKVKIALETMAGKGSELGKTFEELATIIDGVTHNDRLSVCFD
CCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEE
TCHVHDAGYDLVGDLDGVIEQFDRIVGLDRLGVIHVNDSKNVRGAKKDRHENIGYGEIGF
EHEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHCCCCCCCCCCH
DTLNRVIHHEAFAHLPKILETPYIPISEKTKVAPYKQEIEMFRTNQFDAEWRERFTLAHQ
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MLKIGSHVSVSGKKMLLAGSEEAVSYGANTMMVYTGAPQNTRRKPIEDLNIEAALKHMAA
CCCCCCEEEECCCEEEEECCHHHHHCCCCEEEEEECCCCCCCCCCCHHCCHHHHHHHHHH
HDIEEIVVHAPYIINLGNTTKPETFELAVSFLTQELKRAEALQVARHVVLHPGAHVGAGE
HHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
EIGLARIIEGLNEVLTGDEKVKIALETMAGKGSELGKTFEELATIIDGVTHNDRLSVCFD
CCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEE
TCHVHDAGYDLVGDLDGVIEQFDRIVGLDRLGVIHVNDSKNVRGAKKDRHENIGYGEIGF
EHEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHCCCCCCCCCCH
DTLNRVIHHEAFAHLPKILETPYIPISEKTKVAPYKQEIEMFRTNQFDAEWRERFTLAHQ
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA