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The map label for this gene is ppx [H]

Identifier: 229916308

GI number: 229916308

Start: 526448

End: 527977

Strand: Direct

Name: ppx [H]

Synonym: EAT1b_0577

Alternate gene names: 229916308

Gene position: 526448-527977 (Clockwise)

Preceding gene: 229916303

Following gene: 229916309

Centisome position: 17.55

GC content: 45.29

Gene sequence:

>1530_bases
ATGGAAAAGAAACTGGCAGTTATTGATATCGGATCCAACTCGATTCGAAACGTCATCTTCGAAGTGAACGAGGAAGGACA
ATACTTCGAACGGTTAAATGTGAAAGAAGTCGCGCGATTGTCCAGCCATATTAGTGATGCGAATGAAATGACTGAAGAAG
GAATCGCTTCACTCATCGATACGTTACGTCACTTTAAAGAGTTGAACGACCATCATCACGTTACCGATGTGCTCCCAGTA
GCGACAGCAGCCATTCGAAACGCAAATAACTCAGATGAGATTTTGCAGAATGTCAAACAGTCTACGGGGATGACCATCCG
TCTGTTGAGCGATTACGAAGAAGCCTTTTATGGATATTCGGCCATCGTCAACTCCACCTATGTGGAAGATGGATATTCCG
TCGATATTGGTGGGGGATCAATGGAAGTCACATATTTCCAGAACCGAGAGCTCGTCTTTTATCACAGTTTTCCGTTCGGT
GCCGTGACGTTAACGAAAGAATTTATGAAAGATGAGACGATGACGAAAGACGAGCGGAAAGCGTTGACCACATACTTAAA
GAAGGCATTCAAAGAAATTGAATGGCTCGAACCACATGGTGTCCCACTTGTCGGTGTCGGTGGGACGGCTCGTAACTTAG
TCCGGGTCGAACAGACCATATCGAACTTCCCGCTTGAAGGAATCCATCAGTATACGATTTCTGCTGAACAGTTAGAGGGC
GTCATAGATGAACTCGTCGGAATGTCGAGCAAACAACTCGGACGTTTGGATGGGCTGTCAAAAGACCGGGTCGACATCAT
CGCGCCTGCCGTGACAGCAATCGGCGAACTCGCTAAATATTTGATAGTACCTGAGTTCACGATGAGTAACAATGGACTCC
GTGAAGGTCTCTTCTATGAAAACTATTTACATGAGCATGATAAAGTTCGTTTTGCCGATGTGAAGGAAGAAAGTTTTCGT
CATTTTGAAAAACAATACGACGTGAATCCGACTCGTCATCAACATCTCATCCGATTGGCTAGACAGTTGTATACCGGATT
AATTGAAGTGAAGGCGATTAAACCGAAACCATTTGAGCCTGGTCTTCTTGAAGGTGCCTGCCGCCTTGCCTATGTCGGTG
AATATATCGATCACAACAATAAAAGTGACCACACGTTCCATCTGATTACCACAAACGATTTCAAAGGACTAAACAACGAG
GATCGACTCGCTCTTGCCCTCGTCACATCCTTCAAATCAAAACGAGTATTGGAACAACATATCGAGGCTTTCCCGAAATG
GTTTGATCAAAAGTTATTGAAGAGTTTGGAGTTGCTCGGGTCGATTGTGAAACTCGTCGATGCCCTCGACTTGACGGAGC
GTCAGATCGTGACGGACATCCATGTTGCTAGTACAAAGGACGGTCTCACACTTACGCTAGATACGAATGGTTCCGCACGT
TTTGAGTTGCAGCGAAGCACTCGCCATAAAAAGCATTTAGAGCGCGCAATTGATCAATCGATTGAGATTGTCTTGAGCGA
GGAGGAGTAA

Upstream 100 bases:

>100_bases
GCAAGACCGGTTTCATCTACCTAAATGTTAAAATTCACGTAAATCGTATGTTCGTTCAAAACCTTGTCGATTACACTAGT
ATTATGAAGGGGATGGTTTT

Downstream 100 bases:

>100_bases
CCATGTCCGAATATACGAATGAACATTTTAACAACCGTGAACTCAGCTGGCTCGACTTTAACGAGCGTGTCCTCGAGGAG
GCGATGGATGAACGAAATCC

Product: Ppx/GppA phosphatase

Products: NA

Alternate protein names: ExopolyPase; Metaphosphatase [H]

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MEKKLAVIDIGSNSIRNVIFEVNEEGQYFERLNVKEVARLSSHISDANEMTEEGIASLIDTLRHFKELNDHHHVTDVLPV
ATAAIRNANNSDEILQNVKQSTGMTIRLLSDYEEAFYGYSAIVNSTYVEDGYSVDIGGGSMEVTYFQNRELVFYHSFPFG
AVTLTKEFMKDETMTKDERKALTTYLKKAFKEIEWLEPHGVPLVGVGGTARNLVRVEQTISNFPLEGIHQYTISAEQLEG
VIDELVGMSSKQLGRLDGLSKDRVDIIAPAVTAIGELAKYLIVPEFTMSNNGLREGLFYENYLHEHDKVRFADVKEESFR
HFEKQYDVNPTRHQHLIRLARQLYTGLIEVKAIKPKPFEPGLLEGACRLAYVGEYIDHNNKSDHTFHLITTNDFKGLNNE
DRLALALVTSFKSKRVLEQHIEAFPKWFDQKLLKSLELLGSIVKLVDALDLTERQIVTDIHVASTKDGLTLTLDTNGSAR
FELQRSTRHKKHLERAIDQSIEIVLSEEE

Sequences:

>Translated_509_residues
MEKKLAVIDIGSNSIRNVIFEVNEEGQYFERLNVKEVARLSSHISDANEMTEEGIASLIDTLRHFKELNDHHHVTDVLPV
ATAAIRNANNSDEILQNVKQSTGMTIRLLSDYEEAFYGYSAIVNSTYVEDGYSVDIGGGSMEVTYFQNRELVFYHSFPFG
AVTLTKEFMKDETMTKDERKALTTYLKKAFKEIEWLEPHGVPLVGVGGTARNLVRVEQTISNFPLEGIHQYTISAEQLEG
VIDELVGMSSKQLGRLDGLSKDRVDIIAPAVTAIGELAKYLIVPEFTMSNNGLREGLFYENYLHEHDKVRFADVKEESFR
HFEKQYDVNPTRHQHLIRLARQLYTGLIEVKAIKPKPFEPGLLEGACRLAYVGEYIDHNNKSDHTFHLITTNDFKGLNNE
DRLALALVTSFKSKRVLEQHIEAFPKWFDQKLLKSLELLGSIVKLVDALDLTERQIVTDIHVASTKDGLTLTLDTNGSAR
FELQRSTRHKKHLERAIDQSIEIVLSEEE
>Mature_509_residues
MEKKLAVIDIGSNSIRNVIFEVNEEGQYFERLNVKEVARLSSHISDANEMTEEGIASLIDTLRHFKELNDHHHVTDVLPV
ATAAIRNANNSDEILQNVKQSTGMTIRLLSDYEEAFYGYSAIVNSTYVEDGYSVDIGGGSMEVTYFQNRELVFYHSFPFG
AVTLTKEFMKDETMTKDERKALTTYLKKAFKEIEWLEPHGVPLVGVGGTARNLVRVEQTISNFPLEGIHQYTISAEQLEG
VIDELVGMSSKQLGRLDGLSKDRVDIIAPAVTAIGELAKYLIVPEFTMSNNGLREGLFYENYLHEHDKVRFADVKEESFR
HFEKQYDVNPTRHQHLIRLARQLYTGLIEVKAIKPKPFEPGLLEGACRLAYVGEYIDHNNKSDHTFHLITTNDFKGLNNE
DRLALALVTSFKSKRVLEQHIEAFPKWFDQKLLKSLELLGSIVKLVDALDLTERQIVTDIHVASTKDGLTLTLDTNGSAR
FELQRSTRHKKHLERAIDQSIEIVLSEEE

Specific function: Degradation of inorganic polyphosphates. Orthophosphate is released progressively from the ends of polyphosphate of circa 500 residues long, while chains of circa 15 residues compete poorly with polyphosphate as substrate [H]

COG id: COG0248

COG function: function code FP; Exopolyphosphatase

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gppA/ppx family [H]

Homologues:

Organism=Escherichia coli, GI1788848, Length=446, Percent_Identity=24.2152466367713, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI48994966, Length=414, Percent_Identity=23.1884057971014, Blast_Score=94, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6321185, Length=343, Percent_Identity=25.9475218658892, Blast_Score=71, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022371
- InterPro:   IPR003695 [H]

Pfam domain/function: PF02541 Ppx-GppA [H]

EC number: =3.6.1.11 [H]

Molecular weight: Translated: 57831; Mature: 57831

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKKLAVIDIGSNSIRNVIFEVNEEGQYFERLNVKEVARLSSHISDANEMTEEGIASLID
CCCCEEEEECCCCHHHHHHEEECCCCHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHH
TLRHFKELNDHHHVTDVLPVATAAIRNANNSDEILQNVKQSTGMTIRLLSDYEEAFYGYS
HHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEHHHHHHHHHHHH
AIVNSTYVEDGYSVDIGGGSMEVTYFQNRELVFYHSFPFGAVTLTKEFMKDETMTKDERK
HHHCCCEECCCCEEEECCCEEEEEEEECCEEEEEECCCCCHHHHHHHHHHHHCCCHHHHH
ALTTYLKKAFKEIEWLEPHGVPLVGVGGTARNLVRVEQTISNFPLEGIHQYTISAEQLEG
HHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHH
VIDELVGMSSKQLGRLDGLSKDRVDIIAPAVTAIGELAKYLIVPEFTMSNNGLREGLFYE
HHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCHHH
NYLHEHDKVRFADVKEESFRHFEKQYDVNPTRHQHLIRLARQLYTGLIEVKAIKPKPFEP
HHHHHCCCEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCC
GLLEGACRLAYVGEYIDHNNKSDHTFHLITTNDFKGLNNEDRLALALVTSFKSKRVLEQH
CHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCEEHHHHHHHHHHHHHHHH
IEAFPKWFDQKLLKSLELLGSIVKLVDALDLTERQIVTDIHVASTKDGLTLTLDTNGSAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHEEEECCCCCEEEEEECCCCEE
FELQRSTRHKKHLERAIDQSIEIVLSEEE
EEEHHHHHHHHHHHHHHCCCEEEEEECCC
>Mature Secondary Structure
MEKKLAVIDIGSNSIRNVIFEVNEEGQYFERLNVKEVARLSSHISDANEMTEEGIASLID
CCCCEEEEECCCCHHHHHHEEECCCCHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHH
TLRHFKELNDHHHVTDVLPVATAAIRNANNSDEILQNVKQSTGMTIRLLSDYEEAFYGYS
HHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEHHHHHHHHHHHH
AIVNSTYVEDGYSVDIGGGSMEVTYFQNRELVFYHSFPFGAVTLTKEFMKDETMTKDERK
HHHCCCEECCCCEEEECCCEEEEEEEECCEEEEEECCCCCHHHHHHHHHHHHCCCHHHHH
ALTTYLKKAFKEIEWLEPHGVPLVGVGGTARNLVRVEQTISNFPLEGIHQYTISAEQLEG
HHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHH
VIDELVGMSSKQLGRLDGLSKDRVDIIAPAVTAIGELAKYLIVPEFTMSNNGLREGLFYE
HHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCHHH
NYLHEHDKVRFADVKEESFRHFEKQYDVNPTRHQHLIRLARQLYTGLIEVKAIKPKPFEP
HHHHHCCCEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCC
GLLEGACRLAYVGEYIDHNNKSDHTFHLITTNDFKGLNNEDRLALALVTSFKSKRVLEQH
CHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCEEHHHHHHHHHHHHHHHH
IEAFPKWFDQKLLKSLELLGSIVKLVDALDLTERQIVTDIHVASTKDGLTLTLDTNGSAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHEEEECCCCCEEEEEECCCCEE
FELQRSTRHKKHLERAIDQSIEIVLSEEE
EEEHHHHHHHHHHHHHHCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]