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The map label for this gene is ispG

Identifier: 229916302

GI number: 229916302

Start: 520992

End: 522113

Strand: Direct

Name: ispG

Synonym: EAT1b_0571

Alternate gene names: 229916302

Gene position: 520992-522113 (Clockwise)

Preceding gene: 229916300

Following gene: 229916303

Centisome position: 17.37

GC content: 49.29

Gene sequence:

>1122_bases
ATGTCAGAAATTTTGCATCGTTCATTAACACGTCCGGTCCGCGTCGGTAACTTGATTATCGGAGGATCGAACGAAGTCAT
TATTCAATCTATGACCACTACGAAAACGCATGACGTCGAGGCGACAGTCGCCGAGATCAAACGTCTTGAAGAAGCTGGTT
GCCAAGTCGTTCGCGTGGCTTGTCCAGACGAACGTGCAGCTGACGCACTTGCAGAAATTAAGAGCCGCATCAACATCCCG
CTCGTCGTCGACATTCATTTCGACTACAAGCTTGCTTTAAAAGCCATCGAAAGTGGCGTCGATAAAATCCGGATCAACCC
GGGTAACATCGGCCGCCGTGAGAAAGTAGAGGCCGTCGTCAACGCGGCGAAAGCAAAAGGGATCCCAATTCGAATCGGGG
TCAACGCCGGTTCATTAGAAAAACAATTCCTTGAAAAGTATGGATATCCGACCGCGCAAGGTATGGTCGAGAGTGCGATG
CACCACGTGAAGATTCTCGAGGACCTAGGCTTCTACAACACGATCATCTCGCTCAAAGCTTCAGACGTAAACCTTGCTCT
TGAAGCCTACACGCTCGCTGCGAAGACGTTCGATTACCCACTTCACGTCGGAATCACAGAGTCAGGTCCACTCTTCTCCG
GTTCACTCAAATCGGCTGCGGGACTCGGTGCGATTCTGTCTCTTGGAATCGGTTCAACGGTTCGGGTCTCACTTTCAGAT
GACCCGGTTGAAGAAGTAAAAGTTGCGAAAGAAGTGCTCAAGTCATTCGGACTTGCGGCAAATGCCGCGACTTTGATCTC
TTGTCCGACATGCGGACGAATTGAAATTGATTTGATTTCGATTGCAAAAGAGGTAGAAGAATACATCCAAAACATCAACG
TCAACATTAAAGTCGCCGTTCTCGGATGTGCGGTAAACGGACCAGGTGAGGCTCGTGAAGCAGACATTGGAATCGCAGGC
GCACGTAATGAAGGACTTCTCTTCCGTCACGGAAAGATTATCCGCAAAGTGCCTGAGGCGACGATGGTCGAAGAATTGAA
AAAAGAAATCGATGCGATCGCCGCTGAAAAAATGGCGGAACGTGAGCGCGAAGAACAAGAGCTCGCAAACCAATCAAATT
AA

Upstream 100 bases:

>100_bases
GCATCCTAGATGCTTTTTCACATTCTTTTCGTTATGATAATGAAGAAACCTTATCATCTTAAATCGATTGGATTCTTTCC
AAAAGAAGGAGATTACGTAT

Downstream 100 bases:

>100_bases
TGAATCTCATGCCCGTACGGTTGCAACCGTACGGGTTTTTGATAGAATAGAGGGCATGTGAGGAGGAGTGATGAGATGCG
AATCGGCATTGATTTGGATG

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MSEILHRSLTRPVRVGNLIIGGSNEVIIQSMTTTKTHDVEATVAEIKRLEEAGCQVVRVACPDERAADALAEIKSRINIP
LVVDIHFDYKLALKAIESGVDKIRINPGNIGRREKVEAVVNAAKAKGIPIRIGVNAGSLEKQFLEKYGYPTAQGMVESAM
HHVKILEDLGFYNTIISLKASDVNLALEAYTLAAKTFDYPLHVGITESGPLFSGSLKSAAGLGAILSLGIGSTVRVSLSD
DPVEEVKVAKEVLKSFGLAANAATLISCPTCGRIEIDLISIAKEVEEYIQNINVNIKVAVLGCAVNGPGEAREADIGIAG
ARNEGLLFRHGKIIRKVPEATMVEELKKEIDAIAAEKMAEREREEQELANQSN

Sequences:

>Translated_373_residues
MSEILHRSLTRPVRVGNLIIGGSNEVIIQSMTTTKTHDVEATVAEIKRLEEAGCQVVRVACPDERAADALAEIKSRINIP
LVVDIHFDYKLALKAIESGVDKIRINPGNIGRREKVEAVVNAAKAKGIPIRIGVNAGSLEKQFLEKYGYPTAQGMVESAM
HHVKILEDLGFYNTIISLKASDVNLALEAYTLAAKTFDYPLHVGITESGPLFSGSLKSAAGLGAILSLGIGSTVRVSLSD
DPVEEVKVAKEVLKSFGLAANAATLISCPTCGRIEIDLISIAKEVEEYIQNINVNIKVAVLGCAVNGPGEAREADIGIAG
ARNEGLLFRHGKIIRKVPEATMVEELKKEIDAIAAEKMAEREREEQELANQSN
>Mature_372_residues
SEILHRSLTRPVRVGNLIIGGSNEVIIQSMTTTKTHDVEATVAEIKRLEEAGCQVVRVACPDERAADALAEIKSRINIPL
VVDIHFDYKLALKAIESGVDKIRINPGNIGRREKVEAVVNAAKAKGIPIRIGVNAGSLEKQFLEKYGYPTAQGMVESAMH
HVKILEDLGFYNTIISLKASDVNLALEAYTLAAKTFDYPLHVGITESGPLFSGSLKSAAGLGAILSLGIGSTVRVSLSDD
PVEEVKVAKEVLKSFGLAANAATLISCPTCGRIEIDLISIAKEVEEYIQNINVNIKVAVLGCAVNGPGEAREADIGIAGA
RNEGLLFRHGKIIRKVPEATMVEELKKEIDAIAAEKMAEREREEQELANQSN

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=365, Percent_Identity=48.4931506849315, Blast_Score=340, Evalue=8e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_EXISA (C4L3K5)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002884948.1
- GeneID:   7871310
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_0571
- OMA:   IRIAINS
- ProtClustDB:   PRK00366
- HAMAP:   MF_00159
- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF004640
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE; SSF51717 DHP_synth_like

EC number: =1.17.7.1

Molecular weight: Translated: 40140; Mature: 40009

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEILHRSLTRPVRVGNLIIGGSNEVIIQSMTTTKTHDVEATVAEIKRLEEAGCQVVRVA
CCHHHHHHHCCCEEECEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEE
CPDERAADALAEIKSRINIPLVVDIHFDYKLALKAIESGVDKIRINPGNIGRREKVEAVV
CCCHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHH
NAAKAKGIPIRIGVNAGSLEKQFLEKYGYPTAQGMVESAMHHVKILEDLGFYNTIISLKA
HHHHCCCCEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHEEEEEE
SDVNLALEAYTLAAKTFDYPLHVGITESGPLFSGSLKSAAGLGAILSLGIGSTVRVSLSD
CCCEEEEEHEEEHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECC
DPVEEVKVAKEVLKSFGLAANAATLISCPTCGRIEIDLISIAKEVEEYIQNINVNIKVAV
CCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCEEEHHHHHHHHHHHHHHCCCCEEEEEE
LGCAVNGPGEAREADIGIAGARNEGLLFRHGKIIRKVPEATMVEELKKEIDAIAAEKMAE
EEEEECCCCCCCCCCEEEECCCCCCEEEECCHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
REREEQELANQSN
HHHHHHHHHCCCC
>Mature Secondary Structure 
SEILHRSLTRPVRVGNLIIGGSNEVIIQSMTTTKTHDVEATVAEIKRLEEAGCQVVRVA
CHHHHHHHCCCEEECEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEE
CPDERAADALAEIKSRINIPLVVDIHFDYKLALKAIESGVDKIRINPGNIGRREKVEAVV
CCCHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHH
NAAKAKGIPIRIGVNAGSLEKQFLEKYGYPTAQGMVESAMHHVKILEDLGFYNTIISLKA
HHHHCCCCEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHEEEEEE
SDVNLALEAYTLAAKTFDYPLHVGITESGPLFSGSLKSAAGLGAILSLGIGSTVRVSLSD
CCCEEEEEHEEEHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECC
DPVEEVKVAKEVLKSFGLAANAATLISCPTCGRIEIDLISIAKEVEEYIQNINVNIKVAV
CCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCEEEHHHHHHHHHHHHHHCCCCEEEEEE
LGCAVNGPGEAREADIGIAGARNEGLLFRHGKIIRKVPEATMVEELKKEIDAIAAEKMAE
EEEEECCCCCCCCCCEEEECCCCCCEEEECCHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
REREEQELANQSN
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA