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The map label for this gene is ldh2 [H]

Identifier: 229916285

GI number: 229916285

Start: 501626

End: 502582

Strand: Reverse

Name: ldh2 [H]

Synonym: EAT1b_0554

Alternate gene names: 229916285

Gene position: 502582-501626 (Counterclockwise)

Preceding gene: 229916286

Following gene: 229916284

Centisome position: 16.75

GC content: 47.44

Gene sequence:

>957_bases
ATGGAAACATTAAATCATGCTAAAGTAACACGAGTCGCACTTGTAGGGGCAGGGGCAGTAGGTGCGAGTTTCGCTTACCA
ATTGACGACAGCTAGTCTATGTGAAGAACTTGTCATCATCGATATCAATAAAGCGAAGGCAGAAGGAGAAGCGATGGATT
TGAACCATGGGGTTTCATTCGCTTCATCACCGATGCGCGTCTGGGCAGGGGATTACTCGGATTGTGGCGTCGCTGATATC
GTGGTCATCACGGCTGGCGCACCACAGCGTCCAGGTGAAACACGTCTTGACCTCGTTGAAAAGAATGCAAAGATCATGAA
ATCCATGATTTCAGACATTATGGACTCTGGTTTTGACGGAATTATCATCATTGCTTCAAACCCGGTCGACATCATGACTC
ATCTCGCATGGAAATATTCTGGTCTTCCGAAACATCGTGTCTTCGGTTCAGGAACCGTGTTAGATACATCTCGCTTGCGA
TACATGCTCGGTGACTATTTCAACGTCGACCCGCGCAACTGTCATGCCTACATCATGGGTGAGCATGGCGATACGGAGTT
TGCTGCCTGGAGCAACGCGCGTATTTATGGAAAATCAGTCGAACAACTACTTGAAGAGCACGATGAGTACAGCTGGGAAG
ACCTTGAAGATATTTATGTCAACGTTCGTGACGCAGCATATCATATCATCGAACGTAAAGGTGCGACGTACTATGCGATT
GGACTCGGCCTCCTTCGGTTGGTCAAGGCTGTCCTTCGTAACGAGAACACGTTGTTGACAGTCGGTGCCCATCTTGATGG
GGAGTATGGGTTACATGATATCCATATCGGTGTGCCAGCAATCATCAACCGTCAAGGTGTGCGTGAAGTCGTAGAGATTG
AGTTGTCACAAGAAGAGCATGAGAAAATGCAACATTCTGCGAACGTACTCATGAAAACGATGGAACCTGTCTTGTAA

Upstream 100 bases:

>100_bases
GGTTTCCGCTGTAGTTAGAGTTTGTGAATGATGTTACAATCTATTTGTAACAGAGATAACAAAAACATGAAACAGATTGT
TCGAAGGGTGGAGAATCATT

Downstream 100 bases:

>100_bases
TCTCCATCCTTTGGAAACCCGTAGCCATATGGTTACGGGTTTTTATACATATAGAACGAATCATCGTTTGGTCGTGGGTC
GTGTCGTGATTTACAATAGA

Product: L-lactate dehydrogenase

Products: NA

Alternate protein names: L-LDH 2 [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
METLNHAKVTRVALVGAGAVGASFAYQLTTASLCEELVIIDINKAKAEGEAMDLNHGVSFASSPMRVWAGDYSDCGVADI
VVITAGAPQRPGETRLDLVEKNAKIMKSMISDIMDSGFDGIIIIASNPVDIMTHLAWKYSGLPKHRVFGSGTVLDTSRLR
YMLGDYFNVDPRNCHAYIMGEHGDTEFAAWSNARIYGKSVEQLLEEHDEYSWEDLEDIYVNVRDAAYHIIERKGATYYAI
GLGLLRLVKAVLRNENTLLTVGAHLDGEYGLHDIHIGVPAIINRQGVREVVEIELSQEEHEKMQHSANVLMKTMEPVL

Sequences:

>Translated_318_residues
METLNHAKVTRVALVGAGAVGASFAYQLTTASLCEELVIIDINKAKAEGEAMDLNHGVSFASSPMRVWAGDYSDCGVADI
VVITAGAPQRPGETRLDLVEKNAKIMKSMISDIMDSGFDGIIIIASNPVDIMTHLAWKYSGLPKHRVFGSGTVLDTSRLR
YMLGDYFNVDPRNCHAYIMGEHGDTEFAAWSNARIYGKSVEQLLEEHDEYSWEDLEDIYVNVRDAAYHIIERKGATYYAI
GLGLLRLVKAVLRNENTLLTVGAHLDGEYGLHDIHIGVPAIINRQGVREVVEIELSQEEHEKMQHSANVLMKTMEPVL
>Mature_318_residues
METLNHAKVTRVALVGAGAVGASFAYQLTTASLCEELVIIDINKAKAEGEAMDLNHGVSFASSPMRVWAGDYSDCGVADI
VVITAGAPQRPGETRLDLVEKNAKIMKSMISDIMDSGFDGIIIIASNPVDIMTHLAWKYSGLPKHRVFGSGTVLDTSRLR
YMLGDYFNVDPRNCHAYIMGEHGDTEFAAWSNARIYGKSVEQLLEEHDEYSWEDLEDIYVNVRDAAYHIIERKGATYYAI
GLGLLRLVKAVLRNENTLLTVGAHLDGEYGLHDIHIGVPAIINRQGVREVVEIELSQEEHEKMQHSANVLMKTMEPVL

Specific function: Catalyzes The Reversible Oxidation Of Malate To Oxaloacetate. [C]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. LDH family [H]

Homologues:

Organism=Homo sapiens, GI291575128, Length=305, Percent_Identity=38.3606557377049, Blast_Score=237, Evalue=1e-62,
Organism=Homo sapiens, GI4557032, Length=305, Percent_Identity=38.3606557377049, Blast_Score=237, Evalue=1e-62,
Organism=Homo sapiens, GI260099723, Length=305, Percent_Identity=36.7213114754098, Blast_Score=231, Evalue=8e-61,
Organism=Homo sapiens, GI5031857, Length=305, Percent_Identity=36.7213114754098, Blast_Score=231, Evalue=8e-61,
Organism=Homo sapiens, GI9257228, Length=306, Percent_Identity=39.5424836601307, Blast_Score=230, Evalue=2e-60,
Organism=Homo sapiens, GI4504973, Length=306, Percent_Identity=39.5424836601307, Blast_Score=230, Evalue=2e-60,
Organism=Homo sapiens, GI15082234, Length=306, Percent_Identity=36.9281045751634, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI47059044, Length=314, Percent_Identity=36.9426751592357, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI221136809, Length=314, Percent_Identity=36.9426751592357, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI260099727, Length=210, Percent_Identity=39.5238095238095, Blast_Score=179, Evalue=4e-45,
Organism=Homo sapiens, GI260099725, Length=209, Percent_Identity=39.7129186602871, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI207028494, Length=192, Percent_Identity=35.9375, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI103472011, Length=302, Percent_Identity=23.5099337748344, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI103472015, Length=183, Percent_Identity=25.1366120218579, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI21735621, Length=232, Percent_Identity=25, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1789632, Length=320, Percent_Identity=25.3125, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17535107, Length=299, Percent_Identity=36.1204013377926, Blast_Score=225, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI17554310, Length=264, Percent_Identity=26.5151515151515, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320125, Length=163, Percent_Identity=31.2883435582822, Blast_Score=63, Evalue=7e-11,
Organism=Drosophila melanogaster, GI17136226, Length=305, Percent_Identity=38.3606557377049, Blast_Score=239, Evalue=2e-63,
Organism=Drosophila melanogaster, GI45550422, Length=318, Percent_Identity=30.8176100628931, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24647881, Length=321, Percent_Identity=25.8566978193146, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR011304
- InterPro:   IPR018177
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.27 [H]

Molecular weight: Translated: 35162; Mature: 35162

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00064 L_LDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METLNHAKVTRVALVGAGAVGASFAYQLTTASLCEELVIIDINKAKAEGEAMDLNHGVSF
CCCCCHHHEEEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEECCCCCCC
ASSPMRVWAGDYSDCGVADIVVITAGAPQRPGETRLDLVEKNAKIMKSMISDIMDSGFDG
CCCCCEEECCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCE
IIIIASNPVDIMTHLAWKYSGLPKHRVFGSGTVLDTSRLRYMLGDYFNVDPRNCHAYIMG
EEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHCCCCCCCCCCEEEEEEE
EHGDTEFAAWSNARIYGKSVEQLLEEHDEYSWEDLEDIYVNVRDAAYHIIERKGATYYAI
CCCCCCEEECCCCEEEHHHHHHHHHHCCCCCCCCHHHHHEEHHHHHHHHHHCCCCEEEHH
GLGLLRLVKAVLRNENTLLTVGAHLDGEYGLHDIHIGVPAIINRQGVREVVEIELSQEEH
HHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEECCCHHHCHHHHHHHHHHHCCHHHH
EKMQHSANVLMKTMEPVL
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
METLNHAKVTRVALVGAGAVGASFAYQLTTASLCEELVIIDINKAKAEGEAMDLNHGVSF
CCCCCHHHEEEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEECCCCCCC
ASSPMRVWAGDYSDCGVADIVVITAGAPQRPGETRLDLVEKNAKIMKSMISDIMDSGFDG
CCCCCEEECCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCE
IIIIASNPVDIMTHLAWKYSGLPKHRVFGSGTVLDTSRLRYMLGDYFNVDPRNCHAYIMG
EEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHCCCCCCCCCCEEEEEEE
EHGDTEFAAWSNARIYGKSVEQLLEEHDEYSWEDLEDIYVNVRDAAYHIIERKGATYYAI
CCCCCCEEECCCCEEEHHHHHHHHHHCCCCCCCCHHHHHEEHHHHHHHHHHCCCCEEEHH
GLGLLRLVKAVLRNENTLLTVGAHLDGEYGLHDIHIGVPAIINRQGVREVVEIELSQEEH
HHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEECCCHHHCHHHHHHHHHHHCCHHHH
EKMQHSANVLMKTMEPVL
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]