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The map label for this gene is ispA [C]

Identifier: 229916250

GI number: 229916250

Start: 474598

End: 475476

Strand: Reverse

Name: ispA [C]

Synonym: EAT1b_0519

Alternate gene names: 229916250

Gene position: 475476-474598 (Counterclockwise)

Preceding gene: 229916251

Following gene: 229916249

Centisome position: 15.85

GC content: 51.08

Gene sequence:

>879_bases
ATGAACCGCACCGATGACTTGAACAAGTGGAAACGGGCCGTCGAACTGGCGATGCAGGAACTCATTGATTCAAGTGAGAT
GCCGAAACGTCTACGAGACGCAATGGCGTATTCTTTAGAGGCTGGCGGGAAACGCATTCGTCCGGCTCTCGTCTATGCCG
TCCTTGAAACGTATGGCATGTCACTCGAGCGTGGACGAGAAGTGGCGGCGAGTTTGGAGATGGTACATACATACTCACTC
ATCCATGATGATTTGCCGGCCATGGATGATGACGACCTTCGTCGTGGGCGTCCGACCAACCATCGTCAGTTTGATGAAGC
GACCGCTATTTTAGCGGGGGACGCTTTGTTGACGGATGCATTCCGTATTCTCACACAGATGCCACTCTCTGCACTAGATA
TCGTCGAGATCATTCGTATTTTCGGGTTGGCGGCCGGTAGCTCAGGCATGGTTGGTGGGCAGTTGGATGATATGCTGGCT
GAAAAACGAAATGACGTCACACTTGAAGAGCTTCAATCCATTCATGAACGGAAGACAGGTGCGCTACTCGTCTATGCACT
TGAAGCCGGTGCGATTTTGGCAGGCGCTTCTGTGGAAGACCGCATGCACCTCAAACAGTTCGGACGTCATCTCGGCATCG
CTTTTCAGATTCAAGACGATATTCTCGATGTCGTCGGTGATGAAGAAACGATCGGAAAACGCGTCGGCTCAGATGAGTTG
AATGACAAGACGACCTATCCGAAGCTTCTCGGCTTAGAGGGAGCGAAGCAAGCTCTTGACCAAGAAGTGGAAGCGGCACA
AGCTGCAATCGAATCACTTTCTGTTCCAGCGCCGCTGCTTCTTGAGATGCTATCATTTGTAGTGAAACGTAATCATTGA

Upstream 100 bases:

>100_bases
GCTTGTCTGCATTGTGCCAGACAAAACTCTCTGCCGCCGAGAAGAAAATGGATGAAATTTTAGAGCTTGATGGGACGCTT
CGTGAAAAAGGAGGATCCGC

Downstream 100 bases:

>100_bases
CCTCGGTGACACGATGAGCACATCGTGTCACCTTTGTTTTGGGGAATGTGTGGCATATGCCGTCACATTCTTGTAAAATG
TAAGGTACGAATCAGTAAAA

Product: polyprenyl synthetase

Products: NA

Alternate protein names: FPP synthase; (2E,6E)-farnesyl diphosphate synthase; Geranyltranstransferase [H]

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MNRTDDLNKWKRAVELAMQELIDSSEMPKRLRDAMAYSLEAGGKRIRPALVYAVLETYGMSLERGREVAASLEMVHTYSL
IHDDLPAMDDDDLRRGRPTNHRQFDEATAILAGDALLTDAFRILTQMPLSALDIVEIIRIFGLAAGSSGMVGGQLDDMLA
EKRNDVTLEELQSIHERKTGALLVYALEAGAILAGASVEDRMHLKQFGRHLGIAFQIQDDILDVVGDEETIGKRVGSDEL
NDKTTYPKLLGLEGAKQALDQEVEAAQAAIESLSVPAPLLLEMLSFVVKRNH

Sequences:

>Translated_292_residues
MNRTDDLNKWKRAVELAMQELIDSSEMPKRLRDAMAYSLEAGGKRIRPALVYAVLETYGMSLERGREVAASLEMVHTYSL
IHDDLPAMDDDDLRRGRPTNHRQFDEATAILAGDALLTDAFRILTQMPLSALDIVEIIRIFGLAAGSSGMVGGQLDDMLA
EKRNDVTLEELQSIHERKTGALLVYALEAGAILAGASVEDRMHLKQFGRHLGIAFQIQDDILDVVGDEETIGKRVGSDEL
NDKTTYPKLLGLEGAKQALDQEVEAAQAAIESLSVPAPLLLEMLSFVVKRNH
>Mature_292_residues
MNRTDDLNKWKRAVELAMQELIDSSEMPKRLRDAMAYSLEAGGKRIRPALVYAVLETYGMSLERGREVAASLEMVHTYSL
IHDDLPAMDDDDLRRGRPTNHRQFDEATAILAGDALLTDAFRILTQMPLSALDIVEIIRIFGLAAGSSGMVGGQLDDMLA
EKRNDVTLEELQSIHERKTGALLVYALEAGAILAGASVEDRMHLKQFGRHLGIAFQIQDDILDVVGDEETIGKRVGSDEL
NDKTTYPKLLGLEGAKQALDQEVEAAQAAIESLSVPAPLLLEMLSFVVKRNH

Specific function: Unknown

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=240, Percent_Identity=27.5, Blast_Score=82, Evalue=7e-16,
Organism=Escherichia coli, GI1786623, Length=266, Percent_Identity=46.2406015037594, Blast_Score=180, Evalue=9e-47,
Organism=Escherichia coli, GI1789578, Length=213, Percent_Identity=33.8028169014084, Blast_Score=120, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI17505681, Length=248, Percent_Identity=27.8225806451613, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24651612, Length=226, Percent_Identity=29.646017699115, Blast_Score=89, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.10 [H]

Molecular weight: Translated: 32195; Mature: 32195

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRTDDLNKWKRAVELAMQELIDSSEMPKRLRDAMAYSLEAGGKRIRPALVYAVLETYGM
CCCCCHHHHHHHHHHHHHHHHHCHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
SLERGREVAASLEMVHTYSLIHDDLPAMDDDDLRRGRPTNHRQFDEATAILAGDALLTDA
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
FRILTQMPLSALDIVEIIRIFGLAAGSSGMVGGQLDDMLAEKRNDVTLEELQSIHERKTG
HHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCC
ALLVYALEAGAILAGASVEDRMHLKQFGRHLGIAFQIQDDILDVVGDEETIGKRVGSDEL
EEEEEEECCCCEEECCCHHHHHHHHHHHHHHCEEEEEHHHHHHHHCCHHHHHHHCCCCCC
NDKTTYPKLLGLEGAKQALDQEVEAAQAAIESLSVPAPLLLEMLSFVVKRNH
CCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNRTDDLNKWKRAVELAMQELIDSSEMPKRLRDAMAYSLEAGGKRIRPALVYAVLETYGM
CCCCCHHHHHHHHHHHHHHHHHCHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
SLERGREVAASLEMVHTYSLIHDDLPAMDDDDLRRGRPTNHRQFDEATAILAGDALLTDA
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
FRILTQMPLSALDIVEIIRIFGLAAGSSGMVGGQLDDMLAEKRNDVTLEELQSIHERKTG
HHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCC
ALLVYALEAGAILAGASVEDRMHLKQFGRHLGIAFQIQDDILDVVGDEETIGKRVGSDEL
EEEEEEECCCCEEECCCHHHHHHHHHHHHHHCEEEEEHHHHHHHHCCHHHHHHHCCCCCC
NDKTTYPKLLGLEGAKQALDQEVEAAQAAIESLSVPAPLLLEMLSFVVKRNH
CCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8486607; 7918490 [H]