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The map label for this gene is yqiS [H]

Identifier: 229916242

GI number: 229916242

Start: 465017

End: 465913

Strand: Reverse

Name: yqiS [H]

Synonym: EAT1b_0511

Alternate gene names: 229916242

Gene position: 465913-465017 (Counterclockwise)

Preceding gene: 229916243

Following gene: 229916241

Centisome position: 15.53

GC content: 49.39

Gene sequence:

>897_bases
ATGAATTTTGAACGGATGCTGACGAACGCAAAGCAGTTAGAAGGCTGTACCGTGTCCATTGCCGGGGCGGCGGACGAAGA
GGTAATTCGCGCCGTAAAACTCGCTACGGATCATGGATTATCACGCTTCATTTTGTTCGGGGAAGCTCGGGAAATCCGAA
AGTTATGTGAACAGCATGGAATCGACGAGTCAGCCGTCGATATTTTACATGCGAAAGGTGACGTTGAGATTGCGCGACGT
GCCGCATTAGCTGTGAAAAATAAAGAGGCGGACGTCCTGATGAAGGGAATGGTCTCGACATCGACATTTATGAAAGCCGT
CCTCAATCGAGAAGCTGGACTCCGTACGAAACGGACGCTTAGCCACATCGCACTATTCCAAATTCCGAATCGTGAAAAGT
TGATTGGAGTGACGGATGCGGCAATTCATATCGCACCGACACTCGAAGAGAAGGTCGAAATCATTCACAATGCGGTAGAA
GCGATGCATGACATCGGTTACGAATCGCCAAAAGTCGCAGCCATTGGAGCAGTCGAGGTTGTCAATCCACAAATGAATGC
GACTCTCGACGCGGCACTACTTAGCCAAATGAACCGACGTGGACAAATCAAGGGCTGTGTCGTAGATGGACCGCTCGCGC
TCGACAACGCTGTCGACATGGTCGCAGCAAAGCAAAAAGGAATCAACAGTGAAGTTGCCGGACAAGCCGACTTATTAGTC
GTACCTTACATAGAGGTCGGCAACGTCCTTTATAAATCAATCATGTATTTCGCAGAGGCGAGCGTAGCGGCGATTGTCGT
CGGCGCATCTGCCCCTGTCGTATTGACAAGTCGTGCCGATACTGCAGAAGCGAAACTATATTCACTCGCATTCGCTTTGT
TACATGCTAAACATTAA

Upstream 100 bases:

>100_bases
GGTAAGCATTAATTTGCATGGTCATCCGCATTATAAAGCGCTTACAATTTAGGAAACAAACTTGCTTATGATATAGTGTA
GAGAGCAAGGGGGGACAATG

Downstream 100 bases:

>100_bases
TCGTACTTAACAGGAGGAATTAATCATGATGGAAACAAACACAGAGCCACGCTTCCGTATTTTTGATGTCTTACAATCTG
AGGACTACGAGCAAATCGTA

Product: phosphate butyryltransferase

Products: NA

Alternate protein names: Phosphotransbutyrylase [H]

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MNFERMLTNAKQLEGCTVSIAGAADEEVIRAVKLATDHGLSRFILFGEAREIRKLCEQHGIDESAVDILHAKGDVEIARR
AALAVKNKEADVLMKGMVSTSTFMKAVLNREAGLRTKRTLSHIALFQIPNREKLIGVTDAAIHIAPTLEEKVEIIHNAVE
AMHDIGYESPKVAAIGAVEVVNPQMNATLDAALLSQMNRRGQIKGCVVDGPLALDNAVDMVAAKQKGINSEVAGQADLLV
VPYIEVGNVLYKSIMYFAEASVAAIVVGASAPVVLTSRADTAEAKLYSLAFALLHAKH

Sequences:

>Translated_298_residues
MNFERMLTNAKQLEGCTVSIAGAADEEVIRAVKLATDHGLSRFILFGEAREIRKLCEQHGIDESAVDILHAKGDVEIARR
AALAVKNKEADVLMKGMVSTSTFMKAVLNREAGLRTKRTLSHIALFQIPNREKLIGVTDAAIHIAPTLEEKVEIIHNAVE
AMHDIGYESPKVAAIGAVEVVNPQMNATLDAALLSQMNRRGQIKGCVVDGPLALDNAVDMVAAKQKGINSEVAGQADLLV
VPYIEVGNVLYKSIMYFAEASVAAIVVGASAPVVLTSRADTAEAKLYSLAFALLHAKH
>Mature_298_residues
MNFERMLTNAKQLEGCTVSIAGAADEEVIRAVKLATDHGLSRFILFGEAREIRKLCEQHGIDESAVDILHAKGDVEIARR
AALAVKNKEADVLMKGMVSTSTFMKAVLNREAGLRTKRTLSHIALFQIPNREKLIGVTDAAIHIAPTLEEKVEIIHNAVE
AMHDIGYESPKVAAIGAVEVVNPQMNATLDAALLSQMNRRGQIKGCVVDGPLALDNAVDMVAAKQKGINSEVAGQADLLV
VPYIEVGNVLYKSIMYFAEASVAAIVVGASAPVVLTSRADTAEAKLYSLAFALLHAKH

Specific function: Catalyzes the conversion of butyryl-CoA through butyryl phosphate to butyrate [H]

COG id: COG0280

COG function: function code C; Phosphotransacetylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788635, Length=326, Percent_Identity=23.0061349693252, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788800, Length=286, Percent_Identity=25.8741258741259, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 1420 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1250 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012147
- InterPro:   IPR014079
- InterPro:   IPR002505 [H]

Pfam domain/function: PF01515 PTA_PTB [H]

EC number: =2.3.1.19 [H]

Molecular weight: Translated: 31988; Mature: 31988

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFERMLTNAKQLEGCTVSIAGAADEEVIRAVKLATDHGLSRFILFGEAREIRKLCEQHG
CCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHCC
IDESAVDILHAKGDVEIARRAALAVKNKEADVLMKGMVSTSTFMKAVLNREAGLRTKRTL
CCHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
SHIALFQIPNREKLIGVTDAAIHIAPTLEEKVEIIHNAVEAMHDIGYESPKVAAIGAVEV
HHHHEEECCCCCCEEEEECCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEE
VNPQMNATLDAALLSQMNRRGQIKGCVVDGPLALDNAVDMVAAKQKGINSEVAGQADLLV
ECCCCCHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCEEE
VPYIEVGNVLYKSIMYFAEASVAAIVVGASAPVVLTSRADTAEAKLYSLAFALLHAKH
ECHHHHHHHHHHHHHHHHHCCEEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNFERMLTNAKQLEGCTVSIAGAADEEVIRAVKLATDHGLSRFILFGEAREIRKLCEQHG
CCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHCC
IDESAVDILHAKGDVEIARRAALAVKNKEADVLMKGMVSTSTFMKAVLNREAGLRTKRTL
CCHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
SHIALFQIPNREKLIGVTDAAIHIAPTLEEKVEIIHNAVEAMHDIGYESPKVAAIGAVEV
HHHHEEECCCCCCEEEEECCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEE
VNPQMNATLDAALLSQMNRRGQIKGCVVDGPLALDNAVDMVAAKQKGINSEVAGQADLLV
ECCCCCHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCEEE
VPYIEVGNVLYKSIMYFAEASVAAIVVGASAPVVLTSRADTAEAKLYSLAFALLHAKH
ECHHHHHHHHHHHHHHHHHCCEEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]