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The map label for this gene is ldh [H]

Identifier: 229916241

GI number: 229916241

Start: 463870

End: 464991

Strand: Reverse

Name: ldh [H]

Synonym: EAT1b_0510

Alternate gene names: 229916241

Gene position: 464991-463870 (Counterclockwise)

Preceding gene: 229916242

Following gene: 229916240

Centisome position: 15.5

GC content: 50.45

Gene sequence:

>1122_bases
ATGATGGAAACAAACACAGAGCCACGCTTCCGTATTTTTGATGTCTTACAATCTGAGGACTACGAGCAAATCGTATTTTG
CCAAGACCAAGCGTCTGGACTAAAGGCGATCATCGCCATCCACGACACGACGCTCGGACCAGCTCTCGGCGGACTCCGTA
TGTGGAACTATGAATCTGAACAAGAGGCACTCACAGATGTGTTGCGTCTTGCAAAAGGGATGACATACAAGAATGCGGCA
GCGGGTCTCAACCTCGGGGGTGGGAAAGCCGTCATCATCGGAAACGCGAAGACAGATAAATCAGAGGCGCTCTTCCGTGC
GTTCGGACGATACGTTCAATCGCTCAGTGGCCGTTACATCACGGCTGAGGATGTGAATACGACAGTTGCCGACATGGACT
TCATTCACATGGAAACGGATTACGTCACGGGCGTCAGTCCTGCATTCGGTTCAAGTGGTAACCCTTCACCGGTCACGGCA
TATGGTGTGTATCGTGGAATGAAGGCTGCTGCGAAATGGAAATTTGGGACAGATTCGCTCGCAGGGAAGACCGTTGCTGT
ACAAGGTGTTGGTAACGTGGCCTACAACTTATGTCGCCACCTCCACGAAGAGGGTGCTCATTTGATTGTGACTGACATTA
ACGAGGAAGCACTCAAACGTGCCGAAGCAGACTTTGGTGCGACGGTCGTCAAACCGGATGAGATTTACGCGGTCGAATGT
GACATTTTCGCTCCATGTGCTCTCGGTGCCGTCATCAACGACAAAACAATTCCACAATTGAAAGCACAAATCGTCGCAGG
TGCTGCCAACAACCAATTGGCAGAAGATCGTCACGGAGACGTGCTCGACGAAAATGGAATTCTATACGCACCAGACTTCG
TCATTAATGCAGGTGGTGTCATCAACGTCGCAGACGAACTTGAAGGCTACAACCGTGAACGGGCAATGAAGAAAGTCGAA
CTCATCTATGACAACATGATGCGCGTCTTTGAAATCGCAGAACGTGATGGCGTACCAACGCACGTTGCGGCCGATAAGAT
GGCGGAAGAGCGGATTCACATGATGTCGCGTTCACGCAGTCAGTTCCTTAAAGTTGAAAAAAGCATCCTAGGGAGACGCT
AA

Upstream 100 bases:

>100_bases
TTGACAAGTCGTGCCGATACTGCAGAAGCGAAACTATATTCACTCGCATTCGCTTTGTTACATGCTAAACATTAATCGTA
CTTAACAGGAGGAATTAATC

Downstream 100 bases:

>100_bases
GATGACAAAACGGCTAATTCTTGCAATCAACCCAGGGTCGACCTCGACGAAAGTTGGATTGTTTGATGGGAAGGAGGAAG
TGATGACACGCACGGTCCGA

Product: Glu/Leu/Phe/Val dehydrogenase dimerisation region

Products: NA

Alternate protein names: LeuDH [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MMETNTEPRFRIFDVLQSEDYEQIVFCQDQASGLKAIIAIHDTTLGPALGGLRMWNYESEQEALTDVLRLAKGMTYKNAA
AGLNLGGGKAVIIGNAKTDKSEALFRAFGRYVQSLSGRYITAEDVNTTVADMDFIHMETDYVTGVSPAFGSSGNPSPVTA
YGVYRGMKAAAKWKFGTDSLAGKTVAVQGVGNVAYNLCRHLHEEGAHLIVTDINEEALKRAEADFGATVVKPDEIYAVEC
DIFAPCALGAVINDKTIPQLKAQIVAGAANNQLAEDRHGDVLDENGILYAPDFVINAGGVINVADELEGYNRERAMKKVE
LIYDNMMRVFEIAERDGVPTHVAADKMAEERIHMMSRSRSQFLKVEKSILGRR

Sequences:

>Translated_373_residues
MMETNTEPRFRIFDVLQSEDYEQIVFCQDQASGLKAIIAIHDTTLGPALGGLRMWNYESEQEALTDVLRLAKGMTYKNAA
AGLNLGGGKAVIIGNAKTDKSEALFRAFGRYVQSLSGRYITAEDVNTTVADMDFIHMETDYVTGVSPAFGSSGNPSPVTA
YGVYRGMKAAAKWKFGTDSLAGKTVAVQGVGNVAYNLCRHLHEEGAHLIVTDINEEALKRAEADFGATVVKPDEIYAVEC
DIFAPCALGAVINDKTIPQLKAQIVAGAANNQLAEDRHGDVLDENGILYAPDFVINAGGVINVADELEGYNRERAMKKVE
LIYDNMMRVFEIAERDGVPTHVAADKMAEERIHMMSRSRSQFLKVEKSILGRR
>Mature_373_residues
MMETNTEPRFRIFDVLQSEDYEQIVFCQDQASGLKAIIAIHDTTLGPALGGLRMWNYESEQEALTDVLRLAKGMTYKNAA
AGLNLGGGKAVIIGNAKTDKSEALFRAFGRYVQSLSGRYITAEDVNTTVADMDFIHMETDYVTGVSPAFGSSGNPSPVTA
YGVYRGMKAAAKWKFGTDSLAGKTVAVQGVGNVAYNLCRHLHEEGAHLIVTDINEEALKRAEADFGATVVKPDEIYAVEC
DIFAPCALGAVINDKTIPQLKAQIVAGAANNQLAEDRHGDVLDENGILYAPDFVINAGGVINVADELEGYNRERAMKKVE
LIYDNMMRVFEIAERDGVPTHVAADKMAEERIHMMSRSRSQFLKVEKSILGRR

Specific function: Functions catabolically in the bacterial metabolism of branched-chain L-amino acids, and plays an important role in spore germination in cooperation with alanine dehydrogenase [H]

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=304, Percent_Identity=28.9473684210526, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI4885281, Length=304, Percent_Identity=27.9605263157895, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI45549226, Length=305, Percent_Identity=28.8524590163934, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI45553475, Length=305, Percent_Identity=28.8524590163934, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24649283, Length=299, Percent_Identity=26.7558528428094, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24649460, Length=270, Percent_Identity=28.8888888888889, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR016211
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.9 [H]

Molecular weight: Translated: 40782; Mature: 40782

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMETNTEPRFRIFDVLQSEDYEQIVFCQDQASGLKAIIAIHDTTLGPALGGLRMWNYESE
CCCCCCCCCCHHHHHHHCCCCCEEEEECCCCCCCEEEEEEECCCCCHHHCCCEECCCCCH
QEALTDVLRLAKGMTYKNAAAGLNLGGGKAVIIGNAKTDKSEALFRAFGRYVQSLSGRYI
HHHHHHHHHHHHCCCCCHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCEE
TAEDVNTTVADMDFIHMETDYVTGVSPAFGSSGNPSPVTAYGVYRGMKAAAKWKFGTDSL
EECCCCCCHHCCEEEEEECCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCCCC
AGKTVAVQGVGNVAYNLCRHLHEEGAHLIVTDINEEALKRAEADFGATVVKPDEIYAVEC
CCCEEEEECCHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHCCCCEEECCCCEEEEEE
DIFAPCALGAVINDKTIPQLKAQIVAGAANNQLAEDRHGDVLDENGILYAPDFVINAGGV
CCCCCHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEECCCCEEECCCEEEECCCE
INVADELEGYNRERAMKKVELIYDNMMRVFEIAERDGVPTHVAADKMAEERIHMMSRSRS
EEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
QFLKVEKSILGRR
HHHHHHHHHHCCC
>Mature Secondary Structure
MMETNTEPRFRIFDVLQSEDYEQIVFCQDQASGLKAIIAIHDTTLGPALGGLRMWNYESE
CCCCCCCCCCHHHHHHHCCCCCEEEEECCCCCCCEEEEEEECCCCCHHHCCCEECCCCCH
QEALTDVLRLAKGMTYKNAAAGLNLGGGKAVIIGNAKTDKSEALFRAFGRYVQSLSGRYI
HHHHHHHHHHHHCCCCCHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCEE
TAEDVNTTVADMDFIHMETDYVTGVSPAFGSSGNPSPVTAYGVYRGMKAAAKWKFGTDSL
EECCCCCCHHCCEEEEEECCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCCCC
AGKTVAVQGVGNVAYNLCRHLHEEGAHLIVTDINEEALKRAEADFGATVVKPDEIYAVEC
CCCEEEEECCHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHCCCCEEECCCCEEEEEE
DIFAPCALGAVINDKTIPQLKAQIVAGAANNQLAEDRHGDVLDENGILYAPDFVINAGGV
CCCCCHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEECCCCEEECCCEEEECCCE
INVADELEGYNRERAMKKVELIYDNMMRVFEIAERDGVPTHVAADKMAEERIHMMSRSRS
EEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
QFLKVEKSILGRR
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3069133; 1400267 [H]