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The map label for this gene is buk [H]

Identifier: 229916240

GI number: 229916240

Start: 462777

End: 463868

Strand: Reverse

Name: buk [H]

Synonym: EAT1b_0509

Alternate gene names: 229916240

Gene position: 463868-462777 (Counterclockwise)

Preceding gene: 229916241

Following gene: 229916239

Centisome position: 15.46

GC content: 51.74

Gene sequence:

>1092_bases
ATGACAAAACGGCTAATTCTTGCAATCAACCCAGGGTCGACCTCGACGAAAGTTGGATTGTTTGATGGGAAGGAGGAAGT
GATGACACGCACGGTCCGACATGCGATCGATGTGACGGGTCTCACACTTCCAGAGCAGTTGCCTCATCGTGAGCAAGAAG
TACGCGCCTTGTTAGAAGAGACGGGCATCGCGGTCAATGATTTGACCGCGATCGTCGGTCGTGGTGGCTTGCTTGCACCG
ATGACGTCAGGAACGTATACAGTCAACGATCTCATGCGTGAAGATTTGACGAGCGGACGTTTCGGGATGCATGCATCGAA
TCTCGGTGGCCTGATTGCTTCGAAAATCGCTGGGGCTGAAGGGATTCCTAGTTTCATAGTGGATCCAGTCGTCGTCGATG
AGATGGACGAGTTGGCCCGAAAGACCGGCATGCCAGAAATCAATCGTCGTAGCATTTTCCATGCCCTCAATCAAAAGGCT
GTCGCGAAACGATTCGCGGCTGAAAATGGAAGCGATTACAATGAGCTGAATCTTATCGTTGCGCATCTTGGTGGGGGAAT
CACAGTCGGTGCCCATGCGGCAGGTCGAGTCATCGACGTGAATAATGGTCTCGACGGAGATGGACCGCTCGCGCCGGAGC
GGGCAGGCTCGATTCCTGTTGGACAGGTAGTAGAACTATGTTATAGTACCAGGTATAAAGAATCGGAAATGAAAAAGAAA
ATTGTCGGTCAGGGCGGCCTATTTGCTCATCTCGGTACGTTCGATGCCATTGAGATCGAGCAGCGGGTCGAATCAGGTGA
TGATGAGGCGAGACTGTTGTATGAGACGATGGCTTATCGTGTCGCTCAAGAGATTTCAAAACACGGCGCGACATTATGCG
GTCGTGTGGATGCCATCTTACTGACGGGCGGGATCGCATATTCCGAGTGGCTGACTGAAAAAATCAAGGAGCGTGTCACT
TATCTCGCGCCGGTACACGTATATCCGGGTGAAGATGAGCTTAAAGCGTTGGCTGAAGGTGCACTTCGCGTCATCGAGAA
GGAAGAGACCGCACGAGTTTATGAGGGGGATACACATGGCACGAGAATTTGA

Upstream 100 bases:

>100_bases
GTTGCGGCCGATAAGATGGCGGAAGAGCGGATTCACATGATGTCGCGTTCACGCAGTCAGTTCCTTAAAGTTGAAAAAAG
CATCCTAGGGAGACGCTAAG

Downstream 100 bases:

>100_bases
TGTTGTCGTCATCGGTGGAGGACCAGGAGGATATGTGGCTGCAATCAAGGCGGCCCATGCAGGAAAATCTGTCGCAATCG
TCGAGGCGAGAAAGTTAGGC

Product: butyrate kinase

Products: NA

Alternate protein names: BK; Branched-chain carboxylic acid kinase [H]

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MTKRLILAINPGSTSTKVGLFDGKEEVMTRTVRHAIDVTGLTLPEQLPHREQEVRALLEETGIAVNDLTAIVGRGGLLAP
MTSGTYTVNDLMREDLTSGRFGMHASNLGGLIASKIAGAEGIPSFIVDPVVVDEMDELARKTGMPEINRRSIFHALNQKA
VAKRFAAENGSDYNELNLIVAHLGGGITVGAHAAGRVIDVNNGLDGDGPLAPERAGSIPVGQVVELCYSTRYKESEMKKK
IVGQGGLFAHLGTFDAIEIEQRVESGDDEARLLYETMAYRVAQEISKHGATLCGRVDAILLTGGIAYSEWLTEKIKERVT
YLAPVHVYPGEDELKALAEGALRVIEKEETARVYEGDTHGTRI

Sequences:

>Translated_363_residues
MTKRLILAINPGSTSTKVGLFDGKEEVMTRTVRHAIDVTGLTLPEQLPHREQEVRALLEETGIAVNDLTAIVGRGGLLAP
MTSGTYTVNDLMREDLTSGRFGMHASNLGGLIASKIAGAEGIPSFIVDPVVVDEMDELARKTGMPEINRRSIFHALNQKA
VAKRFAAENGSDYNELNLIVAHLGGGITVGAHAAGRVIDVNNGLDGDGPLAPERAGSIPVGQVVELCYSTRYKESEMKKK
IVGQGGLFAHLGTFDAIEIEQRVESGDDEARLLYETMAYRVAQEISKHGATLCGRVDAILLTGGIAYSEWLTEKIKERVT
YLAPVHVYPGEDELKALAEGALRVIEKEETARVYEGDTHGTRI
>Mature_362_residues
TKRLILAINPGSTSTKVGLFDGKEEVMTRTVRHAIDVTGLTLPEQLPHREQEVRALLEETGIAVNDLTAIVGRGGLLAPM
TSGTYTVNDLMREDLTSGRFGMHASNLGGLIASKIAGAEGIPSFIVDPVVVDEMDELARKTGMPEINRRSIFHALNQKAV
AKRFAAENGSDYNELNLIVAHLGGGITVGAHAAGRVIDVNNGLDGDGPLAPERAGSIPVGQVVELCYSTRYKESEMKKKI
VGQGGLFAHLGTFDAIEIEQRVESGDDEARLLYETMAYRVAQEISKHGATLCGRVDAILLTGGIAYSEWLTEKIKERVTY
LAPVHVYPGEDELKALAEGALRVIEKEETARVYEGDTHGTRI

Specific function: Anaerobic degradation of L-threonine to propionate. [C]

COG id: COG3426

COG function: function code C; Butyrate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI145693184, Length=337, Percent_Identity=25.2225519287834, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR011245 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.7 [H]

Molecular weight: Translated: 39202; Mature: 39071

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKRLILAINPGSTSTKVGLFDGKEEVMTRTVRHAIDVTGLTLPEQLPHREQEVRALLEE
CCCEEEEEECCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCHHCCCHHHHHHHHHHH
TGIAVNDLTAIVGRGGLLAPMTSGTYTVNDLMREDLTSGRFGMHASNLGGLIASKIAGAE
HCCCHHHHHHHHCCCCEEEECCCCCEEHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCC
GIPSFIVDPVVVDEMDELARKTGMPEINRRSIFHALNQKAVAKRFAAENGSDYNELNLIV
CCHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHEEE
AHLGGGITVGAHAAGRVIDVNNGLDGDGPLAPERAGSIPVGQVVELCYSTRYKESEMKKK
EECCCCEEECCCCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHH
IVGQGGLFAHLGTFDAIEIEQRVESGDDEARLLYETMAYRVAQEISKHGATLCGRVDAIL
HHCCCCCEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCEEE
LTGGIAYSEWLTEKIKERVTYLAPVHVYPGEDELKALAEGALRVIEKEETARVYEGDTHG
EECCHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCC
TRI
CCC
>Mature Secondary Structure 
TKRLILAINPGSTSTKVGLFDGKEEVMTRTVRHAIDVTGLTLPEQLPHREQEVRALLEE
CCEEEEEECCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCHHCCCHHHHHHHHHHH
TGIAVNDLTAIVGRGGLLAPMTSGTYTVNDLMREDLTSGRFGMHASNLGGLIASKIAGAE
HCCCHHHHHHHHCCCCEEEECCCCCEEHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCC
GIPSFIVDPVVVDEMDELARKTGMPEINRRSIFHALNQKAVAKRFAAENGSDYNELNLIV
CCHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHEEE
AHLGGGITVGAHAAGRVIDVNNGLDGDGPLAPERAGSIPVGQVVELCYSTRYKESEMKKK
EECCCCEEECCCCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHH
IVGQGGLFAHLGTFDAIEIEQRVESGDDEARLLYETMAYRVAQEISKHGATLCGRVDAIL
HHCCCCCEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCEEE
LTGGIAYSEWLTEKIKERVTYLAPVHVYPGEDELKALAEGALRVIEKEETARVYEGDTHG
EECCHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCC
TRI
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA