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The map label for this gene is bfmBB [H]

Identifier: 229916236

GI number: 229916236

Start: 458055

End: 459374

Strand: Reverse

Name: bfmBB [H]

Synonym: EAT1b_0505

Alternate gene names: 229916236

Gene position: 459374-458055 (Counterclockwise)

Preceding gene: 229916237

Following gene: 229916232

Centisome position: 15.31

GC content: 52.12

Gene sequence:

>1320_bases
ATGGAACAAACGATTACCATGCCCCAACTTGGGGAGAGTGTGACTGAAGGCACGATCACGACCTATCTCGTCAAACCGGG
CGACCGTGTGGAAGAGTATGAACCACTCGCGGAAGTCATGACGGATAAAGTGACGGCCGAGATTCCGGCGACTAGTGCGG
GAGTCGTCAAAGAGTTTCTCATTCCGGAAGGGGAGACTGTCAGCGTCGGGACACCGGTATTGACGATGGAAGTTGAAAGT
GCGGAGGAAGCTGTAGTTGAGACGAAGACCGAACCGATTGCAGAAACGACACCTGCAGAACCCGTCTCTAAGCAAGCGGT
CGCCACTACTCCGAAAAAACAGAGTGGAAACGGTCGATACTCACCTGCTGTCATCCGACTCGCCAATGAGAATGATATCG
ATTTGAATGAACTGAGCGGAAGCGGTCTAGGCGGTCGGATCACGCGGAAAGATATTCTTCGTTACTTATCAGAAGGACGT
CCGGCATCGACTCCTGATAAAGCGACACAAGCACCTGTCCAAGAGACGATGGTACAGACGAAGTTGGACGTTCCGACTGA
AGCGCCACGTCCTGTCGTTGAACCGTCACAACCGGTCGCATCATCATCGACGTCATCTGGACGTTATGAATCGATTCCGA
CAGCCGGGGTACGTCAAGCGATTGCGAACAATATGATCCGCTCGAAGCATGAGGCGCCACACGCATGGCTCATGATTGAA
GTGGATGTGACGAACCTGGTTGAAGCCCGTGCGAAATTGAAAGACGAGTTCATGAAACGGGAAGGTGTCAAATTGACGTT
CATGCCGTTCTTCATGAAGGCAGCCATCGAAGCATTGAAGAAGTATCCGATGATGAACTCGGAATGGGCAGGAGATCACA
TCAAAGTTCACCAAGACATCCATCTCTCGGTAGCCGTCGCGGCAAACGACGCCCTTTACGTACCGGTCATTCGCCAAGCG
GATGAGAAGAACATCAAAGGTCTCGCCGTGGCCCTTCAAGACGTTGCAACGCGAGCGCGTGAGAACCGTTTGAAGGCAGA
AGAGATGCGTGGTGGGACGTTCACCATCAACAACACAGGTGCATTTGGTTCGATTCAATCGGCACCGATTCTGAACTACC
CACAAGCGGCAATCCTATCAGTCGAGTCAATCGTGAAGCGTCCAGTATGGATGAATGGAATGTTTGCGGCACGCGATATG
GTCAACCTTTGTATGTCGGTCGACCACCGTGTCCTCGACGGTCTCGTCGCAGGTCAGTTCTTGCAAGCAATCAAACAGTC
GCTCGAGTCGATTGACCCGAACACATATACCGTATATTGA

Upstream 100 bases:

>100_bases
AATGCCGTATGCACCACCGATGGAAAAATTCTTTATCGTATCCCCAGAAAAAATTGCCGAGCGGGCGCGTCAGCTCGCGG
CGTTTTAAGGAGGCATCTCC

Downstream 100 bases:

>100_bases
TTAAAAAATGGAGGCGCTCTCGATCGAGTGCCTCCATTTTTTATGAGTTATAGCCAAGGAATACCGTGAAGACGAAGCTT
GCGACCGAGATCAGTAAGAG

Product: catalytic domain of components of various dehydrogenase complexes

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase [H]

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MEQTITMPQLGESVTEGTITTYLVKPGDRVEEYEPLAEVMTDKVTAEIPATSAGVVKEFLIPEGETVSVGTPVLTMEVES
AEEAVVETKTEPIAETTPAEPVSKQAVATTPKKQSGNGRYSPAVIRLANENDIDLNELSGSGLGGRITRKDILRYLSEGR
PASTPDKATQAPVQETMVQTKLDVPTEAPRPVVEPSQPVASSSTSSGRYESIPTAGVRQAIANNMIRSKHEAPHAWLMIE
VDVTNLVEARAKLKDEFMKREGVKLTFMPFFMKAAIEALKKYPMMNSEWAGDHIKVHQDIHLSVAVAANDALYVPVIRQA
DEKNIKGLAVALQDVATRARENRLKAEEMRGGTFTINNTGAFGSIQSAPILNYPQAAILSVESIVKRPVWMNGMFAARDM
VNLCMSVDHRVLDGLVAGQFLQAIKQSLESIDPNTYTVY

Sequences:

>Translated_439_residues
MEQTITMPQLGESVTEGTITTYLVKPGDRVEEYEPLAEVMTDKVTAEIPATSAGVVKEFLIPEGETVSVGTPVLTMEVES
AEEAVVETKTEPIAETTPAEPVSKQAVATTPKKQSGNGRYSPAVIRLANENDIDLNELSGSGLGGRITRKDILRYLSEGR
PASTPDKATQAPVQETMVQTKLDVPTEAPRPVVEPSQPVASSSTSSGRYESIPTAGVRQAIANNMIRSKHEAPHAWLMIE
VDVTNLVEARAKLKDEFMKREGVKLTFMPFFMKAAIEALKKYPMMNSEWAGDHIKVHQDIHLSVAVAANDALYVPVIRQA
DEKNIKGLAVALQDVATRARENRLKAEEMRGGTFTINNTGAFGSIQSAPILNYPQAAILSVESIVKRPVWMNGMFAARDM
VNLCMSVDHRVLDGLVAGQFLQAIKQSLESIDPNTYTVY
>Mature_439_residues
MEQTITMPQLGESVTEGTITTYLVKPGDRVEEYEPLAEVMTDKVTAEIPATSAGVVKEFLIPEGETVSVGTPVLTMEVES
AEEAVVETKTEPIAETTPAEPVSKQAVATTPKKQSGNGRYSPAVIRLANENDIDLNELSGSGLGGRITRKDILRYLSEGR
PASTPDKATQAPVQETMVQTKLDVPTEAPRPVVEPSQPVASSSTSSGRYESIPTAGVRQAIANNMIRSKHEAPHAWLMIE
VDVTNLVEARAKLKDEFMKREGVKLTFMPFFMKAAIEALKKYPMMNSEWAGDHIKVHQDIHLSVAVAANDALYVPVIRQA
DEKNIKGLAVALQDVATRARENRLKAEEMRGGTFTINNTGAFGSIQSAPILNYPQAAILSVESIVKRPVWMNGMFAARDM
VNLCMSVDHRVLDGLVAGQFLQAIKQSLESIDPNTYTVY

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=430, Percent_Identity=30.6976744186047, Blast_Score=179, Evalue=7e-45,
Organism=Homo sapiens, GI31711992, Length=448, Percent_Identity=29.9107142857143, Blast_Score=178, Evalue=8e-45,
Organism=Homo sapiens, GI203098816, Length=459, Percent_Identity=29.4117647058824, Blast_Score=169, Evalue=6e-42,
Organism=Homo sapiens, GI203098753, Length=459, Percent_Identity=29.4117647058824, Blast_Score=168, Evalue=9e-42,
Organism=Homo sapiens, GI19923748, Length=252, Percent_Identity=38.0952380952381, Blast_Score=152, Evalue=8e-37,
Organism=Homo sapiens, GI260898739, Length=157, Percent_Identity=35.031847133758, Blast_Score=93, Evalue=4e-19,
Organism=Escherichia coli, GI1786946, Length=430, Percent_Identity=31.3953488372093, Blast_Score=215, Evalue=4e-57,
Organism=Escherichia coli, GI1786305, Length=439, Percent_Identity=31.8906605922551, Blast_Score=178, Evalue=8e-46,
Organism=Caenorhabditis elegans, GI17537937, Length=429, Percent_Identity=28.2051282051282, Blast_Score=171, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17560088, Length=458, Percent_Identity=27.9475982532751, Blast_Score=152, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI25146366, Length=194, Percent_Identity=37.6288659793814, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17538894, Length=333, Percent_Identity=26.7267267267267, Blast_Score=117, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6320352, Length=439, Percent_Identity=31.8906605922551, Blast_Score=184, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6324258, Length=457, Percent_Identity=25.382932166302, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI18859875, Length=442, Percent_Identity=30.316742081448, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI24645909, Length=191, Percent_Identity=39.2670157068063, Blast_Score=145, Evalue=7e-35,
Organism=Drosophila melanogaster, GI20129315, Length=230, Percent_Identity=33.0434782608696, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24582497, Length=231, Percent_Identity=32.9004329004329, Blast_Score=144, Evalue=1e-34,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.168 [H]

Molecular weight: Translated: 47836; Mature: 47836

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQTITMPQLGESVTEGTITTYLVKPGDRVEEYEPLAEVMTDKVTAEIPATSAGVVKEFL
CCCCCCCHHHCCHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
IPEGETVSVGTPVLTMEVESAEEAVVETKTEPIAETTPAEPVSKQAVATTPKKQSGNGRY
CCCCCEEECCCCEEEEEECCHHHHHHHCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCC
SPAVIRLANENDIDLNELSGSGLGGRITRKDILRYLSEGRPASTPDKATQAPVQETMVQT
CCEEEEECCCCCCCHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHCHHHHHHHHH
KLDVPTEAPRPVVEPSQPVASSSTSSGRYESIPTAGVRQAIANNMIRSKHEAPHAWLMIE
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEE
VDVTNLVEARAKLKDEFMKREGVKLTFMPFFMKAAIEALKKYPMMNSEWAGDHIKVHQDI
ECHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCE
HLSVAVAANDALYVPVIRQADEKNIKGLAVALQDVATRARENRLKAEEMRGGTFTINNTG
EEEEEEECCCEEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEECCCC
AFGSIQSAPILNYPQAAILSVESIVKRPVWMNGMFAARDMVNLCMSVDHRVLDGLVAGQF
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQAIKQSLESIDPNTYTVY
HHHHHHHHHHCCCCCEEEC
>Mature Secondary Structure
MEQTITMPQLGESVTEGTITTYLVKPGDRVEEYEPLAEVMTDKVTAEIPATSAGVVKEFL
CCCCCCCHHHCCHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
IPEGETVSVGTPVLTMEVESAEEAVVETKTEPIAETTPAEPVSKQAVATTPKKQSGNGRY
CCCCCEEECCCCEEEEEECCHHHHHHHCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCC
SPAVIRLANENDIDLNELSGSGLGGRITRKDILRYLSEGRPASTPDKATQAPVQETMVQT
CCEEEEECCCCCCCHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHCHHHHHHHHH
KLDVPTEAPRPVVEPSQPVASSSTSSGRYESIPTAGVRQAIANNMIRSKHEAPHAWLMIE
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEE
VDVTNLVEARAKLKDEFMKREGVKLTFMPFFMKAAIEALKKYPMMNSEWAGDHIKVHQDI
ECHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCE
HLSVAVAANDALYVPVIRQADEKNIKGLAVALQDVATRARENRLKAEEMRGGTFTINNTG
EEEEEEECCCEEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEECCCC
AFGSIQSAPILNYPQAAILSVESIVKRPVWMNGMFAARDMVNLCMSVDHRVLDGLVAGQF
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQAIKQSLESIDPNTYTVY
HHHHHHHHHHCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8504804; 8969508; 9384377; 7961792 [H]