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The map label for this gene is rluB [H]

Identifier: 229916174

GI number: 229916174

Start: 406831

End: 407589

Strand: Reverse

Name: rluB [H]

Synonym: EAT1b_0443

Alternate gene names: 229916174

Gene position: 407589-406831 (Counterclockwise)

Preceding gene: 229916175

Following gene: 229916173

Centisome position: 13.59

GC content: 49.01

Gene sequence:

>759_bases
ATGGAACGTTTGCAAAAAGTAATCGCACAAGCGGGGGTCGCCTCGCGTCGGAAAGCGGAAGAAATGATTTTGGATGGTCG
CGTCCGCGTGAACGGAAAAGTCGTCCGTGAACTAGGGACGAAAGTTGGACCGAAGTCAGAGATTGAAGTCGATGGCGTCA
AAATCGAACAAGAAGAGCATGTCTACTATTTACTCTATAAACCTAGTGGCTACGTCTCGACGGTCGATGACGACAAAGGA
CGTAAAGTCATCACAGACCTCGTCCCGCCAGGAGCACGTGTATTCCCGGTCGGTCGTCTCGACTACAACACGACGGGACT
CATCATCTTGACGAACGATGGCGAGTTCTCGAACATGATGACGCACCCGAAATATAAAATTCAAAAGCGTTATGTGGCAA
AAGTGAAGAACATCCCATCATACTTGGCCATCAAACAGCTTGAGTCTGGCGTCGAACTCGATGACGGATTCAAAACGGGT
AGTGCGAAAGTGAAGATGAAGTCTGTGGACCAGAAGAAGAACACAGCCATCCTCGAAATCGTCATCTCGGAAGGTCACAA
TCGTCAAATCCGTCGGATGGTGGAAGCCATTGGTTCAGAGGTCATCAAACTGAAGCGCGAACAGTTCGGTTTCTTGACGC
TCGACGGTTTGAACACGGGCGACTGGCGTGAACTTTCACACAAAGAAATCAGTAAGCTTCGGGAACTTGCGAACCCTACC
GTTCCACCGAAACCACGAGGCAAAAAGAAGAAAAAATAA

Upstream 100 bases:

>100_bases
CCACCATTAACATTTGAGGATGAACTGACAGACGAAGGCGATTTGTTCTTCGGTCAGGAATCACCGAAGCAAGAAGAGAG
ATTGGAGAAGGATTAATCAT

Downstream 100 bases:

>100_bases
CAACAAAAAATCGTCCTGAAATAGGATGATTTTTTTGATTGTGTAGATTGTGTGACAAAAAAATGAAAAAAGTCTGTTAA
TGTGCGTTTTTAATGTTTTT

Product: pseudouridine synthase

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MERLQKVIAQAGVASRRKAEEMILDGRVRVNGKVVRELGTKVGPKSEIEVDGVKIEQEEHVYYLLYKPSGYVSTVDDDKG
RKVITDLVPPGARVFPVGRLDYNTTGLIILTNDGEFSNMMTHPKYKIQKRYVAKVKNIPSYLAIKQLESGVELDDGFKTG
SAKVKMKSVDQKKNTAILEIVISEGHNRQIRRMVEAIGSEVIKLKREQFGFLTLDGLNTGDWRELSHKEISKLRELANPT
VPPKPRGKKKKK

Sequences:

>Translated_252_residues
MERLQKVIAQAGVASRRKAEEMILDGRVRVNGKVVRELGTKVGPKSEIEVDGVKIEQEEHVYYLLYKPSGYVSTVDDDKG
RKVITDLVPPGARVFPVGRLDYNTTGLIILTNDGEFSNMMTHPKYKIQKRYVAKVKNIPSYLAIKQLESGVELDDGFKTG
SAKVKMKSVDQKKNTAILEIVISEGHNRQIRRMVEAIGSEVIKLKREQFGFLTLDGLNTGDWRELSHKEISKLRELANPT
VPPKPRGKKKKK
>Mature_252_residues
MERLQKVIAQAGVASRRKAEEMILDGRVRVNGKVVRELGTKVGPKSEIEVDGVKIEQEEHVYYLLYKPSGYVSTVDDDKG
RKVITDLVPPGARVFPVGRLDYNTTGLIILTNDGEFSNMMTHPKYKIQKRYVAKVKNIPSYLAIKQLESGVELDDGFKTG
SAKVKMKSVDQKKNTAILEIVISEGHNRQIRRMVEAIGSEVIKLKREQFGFLTLDGLNTGDWRELSHKEISKLRELANPT
VPPKPRGKKKKK

Specific function: Responsible for synthesis of pseudouridine from uracil- 2633 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=263, Percent_Identity=38.0228136882129, Blast_Score=148, Evalue=3e-37,
Organism=Escherichia coli, GI1788510, Length=234, Percent_Identity=33.3333333333333, Blast_Score=127, Evalue=9e-31,
Organism=Escherichia coli, GI1790453, Length=237, Percent_Identity=36.2869198312236, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI87081838, Length=185, Percent_Identity=32.4324324324324, Blast_Score=101, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70

Molecular weight: Translated: 28399; Mature: 28399

Theoretical pI: Translated: 10.39; Mature: 10.39

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERLQKVIAQAGVASRRKAEEMILDGRVRVNGKVVRELGTKVGPKSEIEVDGVKIEQEEH
CHHHHHHHHHHCHHHHHHHHHHHHCCEEEECHHHHHHHHHCCCCCCCEEECCEEEECCCC
VYYLLYKPSGYVSTVDDDKGRKVITDLVPPGARVFPVGRLDYNTTGLIILTNDGEFSNMM
EEEEEECCCCCEEECCCCCCCEEEECCCCCCCEEEEECCCCCCCCEEEEEECCCCCCCCC
THPKYKIQKRYVAKVKNIPSYLAIKQLESGVELDDGFKTGSAKVKMKSVDQKKNTAILEI
CCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEE
VISEGHNRQIRRMVEAIGSEVIKLKREQFGFLTLDGLNTGDWRELSHKEISKLRELANPT
EECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCC
VPPKPRGKKKKK
CCCCCCCCCCCC
>Mature Secondary Structure
MERLQKVIAQAGVASRRKAEEMILDGRVRVNGKVVRELGTKVGPKSEIEVDGVKIEQEEH
CHHHHHHHHHHCHHHHHHHHHHHHCCEEEECHHHHHHHHHCCCCCCCEEECCEEEECCCC
VYYLLYKPSGYVSTVDDDKGRKVITDLVPPGARVFPVGRLDYNTTGLIILTNDGEFSNMM
EEEEEECCCCCEEECCCCCCCEEEECCCCCCCEEEEECCCCCCCCEEEEEECCCCCCCCC
THPKYKIQKRYVAKVKNIPSYLAIKQLESGVELDDGFKTGSAKVKMKSVDQKKNTAILEI
CCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEE
VISEGHNRQIRRMVEAIGSEVIKLKREQFGFLTLDGLNTGDWRELSHKEISKLRELANPT
EECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCC
VPPKPRGKKKKK
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934829; 9384377; 9888802 [H]