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The map label for this gene is lexA

Identifier: 229916139

GI number: 229916139

Start: 371706

End: 372326

Strand: Direct

Name: lexA

Synonym: EAT1b_0408

Alternate gene names: 229916139

Gene position: 371706-372326 (Clockwise)

Preceding gene: 229916131

Following gene: 229916140

Centisome position: 12.39

GC content: 50.08

Gene sequence:

>621_bases
ATGAAAAAAATGTCAGCGCGACAACAACAGATTCTTGATTTCATCAAAGACGAAGTACGGGCTAAAGGTTATCCGCCTTC
TGTTCGTGAAATTGGAGAAGCCGTCGGTTTAGCATCTAGTTCGACCGTACACGGTCATCTAGATCGTTTAGAGAAACGCG
GATTGATTCGTCGAGATAAGACGAAGCCTCGCGCGATTGAAATTTTGACTGACGATATGCCGACAATGGAAGAGCAAGAG
TCCGTCATGTACGTACCGGTCATCGGTAAGGTCACTGCCGGGACGCCGATCACCGCCATCGAGAACGTGGAAGAACACTT
CCCGCTCCCGGCACATATCGTCGGGTCAGATAATGTATACATGTTGTCTGTCTCTGGGGATTCGATGATCAACGCAGGCA
TCTTGGACGGGGACCGGGTGCTCGTGCGCCAACAGAGCACGGCCGACAATGGAGAGATTGTCGTCGCCATGACGGAAGAA
GGCGAAGCGACCGTGAAGCGGATTTACAAGGAAGCTTCAAAGGTCCGCCTTCAACCGGAGAACGACGAGTTAGAAGCGAT
GTATTTTGACAACGTCTCGATTTTAGGGAAGGTTATCGGTGTATACCGTACGATTCATTAA

Upstream 100 bases:

>100_bases
ACGAACGAATGTTTGTACGCTCATGTGTTCTTGTGTTACACTAGACACATAAAGAATAGGATGCGATTGCAGGTTATTCG
ATTATATCGAGGTGAACATA

Downstream 100 bases:

>100_bases
TGGCTAGTGGCAAGACCCATACCCGAACGAATCTCGTGGCAACCGGAGCACTCGCGGTTGCCACGCCTTTTCTTGAGATC
GATGTTCCATTCTCTTTGAT

Product: SOS-response transcriptional repressor, LexA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MKKMSARQQQILDFIKDEVRAKGYPPSVREIGEAVGLASSSTVHGHLDRLEKRGLIRRDKTKPRAIEILTDDMPTMEEQE
SVMYVPVIGKVTAGTPITAIENVEEHFPLPAHIVGSDNVYMLSVSGDSMINAGILDGDRVLVRQQSTADNGEIVVAMTEE
GEATVKRIYKEASKVRLQPENDELEAMYFDNVSILGKVIGVYRTIH

Sequences:

>Translated_206_residues
MKKMSARQQQILDFIKDEVRAKGYPPSVREIGEAVGLASSSTVHGHLDRLEKRGLIRRDKTKPRAIEILTDDMPTMEEQE
SVMYVPVIGKVTAGTPITAIENVEEHFPLPAHIVGSDNVYMLSVSGDSMINAGILDGDRVLVRQQSTADNGEIVVAMTEE
GEATVKRIYKEASKVRLQPENDELEAMYFDNVSILGKVIGVYRTIH
>Mature_206_residues
MKKMSARQQQILDFIKDEVRAKGYPPSVREIGEAVGLASSSTVHGHLDRLEKRGLIRRDKTKPRAIEILTDDMPTMEEQE
SVMYVPVIGKVTAGTPITAIENVEEHFPLPAHIVGSDNVYMLSVSGDSMINAGILDGDRVLVRQQSTADNGEIVVAMTEE
GEATVKRIYKEASKVRLQPENDELEAMYFDNVSILGKVIGVYRTIH

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family

Homologues:

Organism=Escherichia coli, GI1790476, Length=208, Percent_Identity=34.6153846153846, Blast_Score=124, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEXA_EXISA (C4L2T2)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002884785.1
- GeneID:   7871144
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_0408
- OMA:   IAENPDF
- ProtClustDB:   CLSK2489288
- GO:   GO:0006508
- GO:   GO:0006350
- HAMAP:   MF_00015
- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00726
- TIGRFAMs:   TIGR00498

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.88

Molecular weight: Translated: 22850; Mature: 22850

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: ACT_SITE 128-128 ACT_SITE 166-166

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKMSARQQQILDFIKDEVRAKGYPPSVREIGEAVGLASSSTVHGHLDRLEKRGLIRRDK
CCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCC
TKPRAIEILTDDMPTMEEQESVMYVPVIGKVTAGTPITAIENVEEHFPLPAHIVGSDNVY
CCCEEEEEEECCCCCCCCCCCEEEEEEECEECCCCCCHHHHHHHHHCCCCEEEECCCCEE
MLSVSGDSMINAGILDGDRVLVRQQSTADNGEIVVAMTEEGEATVKRIYKEASKVRLQPE
EEEECCCCEEECCEECCCEEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHEECCCC
NDELEAMYFDNVSILGKVIGVYRTIH
CCCEEEEEECCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKMSARQQQILDFIKDEVRAKGYPPSVREIGEAVGLASSSTVHGHLDRLEKRGLIRRDK
CCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCC
TKPRAIEILTDDMPTMEEQESVMYVPVIGKVTAGTPITAIENVEEHFPLPAHIVGSDNVY
CCCEEEEEEECCCCCCCCCCCEEEEEEECEECCCCCCHHHHHHHHHCCCCEEEECCCCEE
MLSVSGDSMINAGILDGDRVLVRQQSTADNGEIVVAMTEEGEATVKRIYKEASKVRLQPE
EEEECCCCEEECCEECCCEEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHEECCCC
NDELEAMYFDNVSILGKVIGVYRTIH
CCCEEEEEECCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA