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The map label for this gene is prfC [H]

Identifier: 229916125

GI number: 229916125

Start: 358070

End: 359668

Strand: Reverse

Name: prfC [H]

Synonym: EAT1b_0394

Alternate gene names: 229916125

Gene position: 359668-358070 (Counterclockwise)

Preceding gene: 229916129

Following gene: 229916124

Centisome position: 11.99

GC content: 49.03

Gene sequence:

>1599_bases
ATGAGCCAATTGAAACAGGAAGTCGAGAGACGTCGGATATTCGGCATTATCTCTCACCCGGATGCGGGGAAGACAACATT
AACAGAGAAATTACTTTTGCACGGTGGTGCGATTCGCGAGGCGGGTACGGTCAAAGCGCGCAAAAACTCAAAGCACGCGA
AATCCGACTGGATGGAAATCGAGAAACAACGTGGGATCTCGGTCACATCATCTGTCATGCAATTCATCTATCAAGATAAG
GTCGTATCGATCATGGACACACCAGGACACAACGACTTCGGTGAAGACACGTATCGCGTGTTGACATCGGTTGATAGCGC
GGTCATGGTCATCGATGCTGCGAAAGGGATTGAAACACAGACGAAAAAACTATTCCAAGTTTGTCGGATGCGTGGGATTC
CAATCTTCACATTTATGAACAAGTTGGATCGTCAGGCAAAAGATCCGCTTGAATTGATGGAAGAGCTCGAAGAAGTGTTA
GGCATGCCTTCTGTCGCGATTACATGGCCGATCGGGAGCGGAATGCAGTTTGAAGGTGTCTACGACCGAATGAAAAATGA
GGTCCACTTGTTCCGTGGGGAGAAGTCGACACTTCAACTGAGTGAGGACGGGGTAAACGACCCGGTGCTCGCTGATCATC
TTTCTGAAGAGAACTTGACGAACCTTCGCGATGAGATCGACCTTCTTGACGGAGCGGGGAACGAGATTGATGTCGAGGCG
ATTCAGAGCGGTAAATTGACACCGGTATTTTTCGGAACAGCGCTCGTCGACTTTGGCGTGACTGCGTTCTTAAATCACTA
TTTAGATATGTCTCCGGCTCCTGAGGCCCGTAAGTCGAACGTCGGTCCGATCAATCCGACATCAGAAGACTTCAGTGGAT
TCGTCTTCAAGATTCAAGCGAACATGAACCCGGCACACCGCGACCGCATCGCATTCGTCCGCATCTGTTCCGGTGTGTTC
GAGCGTGGGATGGACGTGACGCTCACCCGGACTGGGAAAAAGATTAAATTGAGCCAATCGACACAATTGATGGCGAACGA
TCGAGAAACAGTCGACCAAGCGTTTGCCGGTGATGTCATCGGGATCTACGATTCTGGGACGTATCAAATTGGAGATACGA
TCACCACGTCGAAGCAAAAGATTGCGTTTGAAGCCTTGCCGACTTTCCCACCGGAATTGTTCATGCGCGTTTCGCCAATC
AACTCGCTCAAGTCGAAACACTTCCATAAAGGGGTAGAACAGCTTGCGCAAGAAGGGGCGATTCAAGTGTATCGCAACGA
ATATAACGAGATTTATCTTGGCGCGGTCGGACAACTTCAATTCGAGGTATTCGAATATCGTTTAAACAATGAATATGGCG
TCGATATTCGAATGGAACCAGTCTCATATAGCGTTGCTCGTTGGGTCAAAGATAAAGAGTTGAAGGCATTGAAACCGTTC
CAAGATTCACGGAACATGCTCGTTACAGACCGTTGGGAACGTCCGGTCTTCCTGTTCGCGAACGAGTTCACATATGAACG
GTTCGCTGAGCGTCACGCAGATGACTTGACGCTTGTTGAGGCACTTGACGTCAACGAAGAGATCGGTACGGAAGAATAA

Upstream 100 bases:

>100_bases
TTCCATTAGGATAGACGAACGGATTTGATTTCGTTAGAATAGATAGCGTTGCTACGAGAATAAGCATGAACTGAGACGAG
AAAGGAAAGGTCTATCCTAT

Downstream 100 bases:

>100_bases
GATGTAAACACCTCGTTTCTGAGGTGTTTTTTTGTTGTGAAGTAAGTCATTTTAGTTTTGATAAGTTTTACTTATGACTA
ATTTGTGACGGAATGTATTT

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 532; Mature: 531

Protein sequence:

>532_residues
MSQLKQEVERRRIFGIISHPDAGKTTLTEKLLLHGGAIREAGTVKARKNSKHAKSDWMEIEKQRGISVTSSVMQFIYQDK
VVSIMDTPGHNDFGEDTYRVLTSVDSAVMVIDAAKGIETQTKKLFQVCRMRGIPIFTFMNKLDRQAKDPLELMEELEEVL
GMPSVAITWPIGSGMQFEGVYDRMKNEVHLFRGEKSTLQLSEDGVNDPVLADHLSEENLTNLRDEIDLLDGAGNEIDVEA
IQSGKLTPVFFGTALVDFGVTAFLNHYLDMSPAPEARKSNVGPINPTSEDFSGFVFKIQANMNPAHRDRIAFVRICSGVF
ERGMDVTLTRTGKKIKLSQSTQLMANDRETVDQAFAGDVIGIYDSGTYQIGDTITTSKQKIAFEALPTFPPELFMRVSPI
NSLKSKHFHKGVEQLAQEGAIQVYRNEYNEIYLGAVGQLQFEVFEYRLNNEYGVDIRMEPVSYSVARWVKDKELKALKPF
QDSRNMLVTDRWERPVFLFANEFTYERFAERHADDLTLVEALDVNEEIGTEE

Sequences:

>Translated_532_residues
MSQLKQEVERRRIFGIISHPDAGKTTLTEKLLLHGGAIREAGTVKARKNSKHAKSDWMEIEKQRGISVTSSVMQFIYQDK
VVSIMDTPGHNDFGEDTYRVLTSVDSAVMVIDAAKGIETQTKKLFQVCRMRGIPIFTFMNKLDRQAKDPLELMEELEEVL
GMPSVAITWPIGSGMQFEGVYDRMKNEVHLFRGEKSTLQLSEDGVNDPVLADHLSEENLTNLRDEIDLLDGAGNEIDVEA
IQSGKLTPVFFGTALVDFGVTAFLNHYLDMSPAPEARKSNVGPINPTSEDFSGFVFKIQANMNPAHRDRIAFVRICSGVF
ERGMDVTLTRTGKKIKLSQSTQLMANDRETVDQAFAGDVIGIYDSGTYQIGDTITTSKQKIAFEALPTFPPELFMRVSPI
NSLKSKHFHKGVEQLAQEGAIQVYRNEYNEIYLGAVGQLQFEVFEYRLNNEYGVDIRMEPVSYSVARWVKDKELKALKPF
QDSRNMLVTDRWERPVFLFANEFTYERFAERHADDLTLVEALDVNEEIGTEE
>Mature_531_residues
SQLKQEVERRRIFGIISHPDAGKTTLTEKLLLHGGAIREAGTVKARKNSKHAKSDWMEIEKQRGISVTSSVMQFIYQDKV
VSIMDTPGHNDFGEDTYRVLTSVDSAVMVIDAAKGIETQTKKLFQVCRMRGIPIFTFMNKLDRQAKDPLELMEELEEVLG
MPSVAITWPIGSGMQFEGVYDRMKNEVHLFRGEKSTLQLSEDGVNDPVLADHLSEENLTNLRDEIDLLDGAGNEIDVEAI
QSGKLTPVFFGTALVDFGVTAFLNHYLDMSPAPEARKSNVGPINPTSEDFSGFVFKIQANMNPAHRDRIAFVRICSGVFE
RGMDVTLTRTGKKIKLSQSTQLMANDRETVDQAFAGDVIGIYDSGTYQIGDTITTSKQKIAFEALPTFPPELFMRVSPIN
SLKSKHFHKGVEQLAQEGAIQVYRNEYNEIYLGAVGQLQFEVFEYRLNNEYGVDIRMEPVSYSVARWVKDKELKALKPFQ
DSRNMLVTDRWERPVFLFANEFTYERFAERHADDLTLVEALDVNEEIGTEE

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=477, Percent_Identity=27.8825995807128, Blast_Score=178, Evalue=1e-44,
Organism=Homo sapiens, GI19923640, Length=557, Percent_Identity=23.1597845601436, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI25306283, Length=405, Percent_Identity=27.1604938271605, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI25306287, Length=305, Percent_Identity=28.8524590163934, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI157426893, Length=186, Percent_Identity=29.0322580645161, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI94966754, Length=148, Percent_Identity=29.0540540540541, Blast_Score=73, Evalue=6e-13,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=28.8590604026846, Blast_Score=66, Evalue=9e-11,
Organism=Escherichia coli, GI1790835, Length=519, Percent_Identity=47.7842003853565, Blast_Score=495, Evalue=1e-141,
Organism=Escherichia coli, GI1789738, Length=488, Percent_Identity=27.6639344262295, Blast_Score=148, Evalue=9e-37,
Organism=Escherichia coli, GI48994988, Length=142, Percent_Identity=35.2112676056338, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI1788922, Length=183, Percent_Identity=26.775956284153, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17533571, Length=486, Percent_Identity=26.5432098765432, Blast_Score=177, Evalue=8e-45,
Organism=Caenorhabditis elegans, GI17556745, Length=483, Percent_Identity=23.6024844720497, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17557151, Length=178, Percent_Identity=26.4044943820225, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71988819, Length=136, Percent_Identity=28.6764705882353, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71988811, Length=136, Percent_Identity=28.6764705882353, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323098, Length=478, Percent_Identity=26.7782426778243, Blast_Score=172, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6322359, Length=529, Percent_Identity=24.7637051039698, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6323320, Length=178, Percent_Identity=29.7752808988764, Blast_Score=80, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6324707, Length=160, Percent_Identity=27.5, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320593, Length=160, Percent_Identity=27.5, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24582462, Length=482, Percent_Identity=28.6307053941909, Blast_Score=204, Evalue=1e-52,
Organism=Drosophila melanogaster, GI221458488, Length=517, Percent_Identity=24.9516441005803, Blast_Score=135, Evalue=1e-31,
Organism=Drosophila melanogaster, GI78706572, Length=159, Percent_Identity=32.0754716981132, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24585711, Length=168, Percent_Identity=27.9761904761905, Blast_Score=66, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24585713, Length=168, Percent_Identity=27.9761904761905, Blast_Score=66, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24585709, Length=168, Percent_Identity=27.9761904761905, Blast_Score=66, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 60099; Mature: 59968

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQLKQEVERRRIFGIISHPDAGKTTLTEKLLLHGGAIREAGTVKARKNSKHAKSDWMEI
CCHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHH
EKQRGISVTSSVMQFIYQDKVVSIMDTPGHNDFGEDTYRVLTSVDSAVMVIDAAKGIETQ
HHHCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCHH
TKKLFQVCRMRGIPIFTFMNKLDRQAKDPLELMEELEEVLGMPSVAITWPIGSGMQFEGV
HHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEECCCCCCEEHHH
YDRMKNEVHLFRGEKSTLQLSEDGVNDPVLADHLSEENLTNLRDEIDLLDGAGNEIDVEA
HHHCCCCEEEEECCCCEEEECCCCCCCCHHHHHCCCCHHHHHHHHHHHHCCCCCEEEHHH
IQSGKLTPVFFGTALVDFGVTAFLNHYLDMSPAPEARKSNVGPINPTSEDFSGFVFKIQA
HCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCCCCCCCCCEEEEEEE
NMNPAHRDRIAFVRICSGVFERGMDVTLTRTGKKIKLSQSTQLMANDRETVDQAFAGDVI
CCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEECCCHHHHHCCHHHHHHHHCCCEE
GIYDSGTYQIGDTITTSKQKIAFEALPTFPPELFMRVSPINSLKSKHFHKGVEQLAQEGA
EEECCCCEEECCCEECCCHHEEEECCCCCCHHHHEEECCHHHHHHHHHHHHHHHHHHCCC
IQVYRNEYNEIYLGAVGQLQFEVFEYRLNNEYGVDIRMEPVSYSVARWVKDKELKALKPF
CEEECCCCCEEEEECCCCEEEEEEEEEECCCCCCEEEECCCHHHHHHHHCCCHHHHCCCC
QDSRNMLVTDRWERPVFLFANEFTYERFAERHADDLTLVEALDVNEEIGTEE
CCCCCEEEEECCCCCEEEEECCHHHHHHHHHCCCCEEEHHHCCCCHHCCCCC
>Mature Secondary Structure 
SQLKQEVERRRIFGIISHPDAGKTTLTEKLLLHGGAIREAGTVKARKNSKHAKSDWMEI
CHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHH
EKQRGISVTSSVMQFIYQDKVVSIMDTPGHNDFGEDTYRVLTSVDSAVMVIDAAKGIETQ
HHHCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCHH
TKKLFQVCRMRGIPIFTFMNKLDRQAKDPLELMEELEEVLGMPSVAITWPIGSGMQFEGV
HHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEECCCCCCEEHHH
YDRMKNEVHLFRGEKSTLQLSEDGVNDPVLADHLSEENLTNLRDEIDLLDGAGNEIDVEA
HHHCCCCEEEEECCCCEEEECCCCCCCCHHHHHCCCCHHHHHHHHHHHHCCCCCEEEHHH
IQSGKLTPVFFGTALVDFGVTAFLNHYLDMSPAPEARKSNVGPINPTSEDFSGFVFKIQA
HCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCCCCCCCCCEEEEEEE
NMNPAHRDRIAFVRICSGVFERGMDVTLTRTGKKIKLSQSTQLMANDRETVDQAFAGDVI
CCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEECCCHHHHHCCHHHHHHHHCCCEE
GIYDSGTYQIGDTITTSKQKIAFEALPTFPPELFMRVSPINSLKSKHFHKGVEQLAQEGA
EEECCCCEEECCCEECCCHHEEEECCCCCCHHHHEEECCHHHHHHHHHHHHHHHHHHCCC
IQVYRNEYNEIYLGAVGQLQFEVFEYRLNNEYGVDIRMEPVSYSVARWVKDKELKALKPF
CEEECCCCCEEEEECCCCEEEEEEEEEECCCCCCEEEECCCHHHHHHHHCCCHHHHCCCC
QDSRNMLVTDRWERPVFLFANEFTYERFAERHADDLTLVEALDVNEEIGTEE
CCCCCEEEEECCCCCEEEEECCHHHHHHHHHCCCCEEEHHHCCCCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA