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The map label for this gene is opuD [H]

Identifier: 229916122

GI number: 229916122

Start: 352862

End: 354427

Strand: Reverse

Name: opuD [H]

Synonym: EAT1b_0391

Alternate gene names: 229916122

Gene position: 354427-352862 (Counterclockwise)

Preceding gene: 229916123

Following gene: 229916121

Centisome position: 11.81

GC content: 47.25

Gene sequence:

>1566_bases
ATGGAACGAAAAAGCCGGGTGACGACCGTATTCGTCGTATCTGCAATCATAACTGGCTTATTTACGATTTGGGGACTTTT
CCCCGAGAGTTTATTAGGTAATGCCAGTCTATTAAACGTGACGAGTACGTTACAAGGCTGGCTCTCAAACGGGATGGGTT
GGTTCTATCTCTTGAGCGCCACCGGGTTTTTACTGGTTGCCATCTTTTTAATCTTTTCAAGATATGGTTCGATTCGACTT
GGAAAGGATACAGATCGTCCAGAATTTAGTTATTTATCATGGTTTGCCATGTTATTCAGTGCCGGGATGGGAATTGGACT
CATCTTCTGGGGAGCGGCTGAACCGTTGCTCCACTTCCACTCACCACCATTCGCTTCCCCTACACCTGAAGGAGATGCAC
GGACTGCGATGCGTTATGCATTTTTCCATTGGGGCCTCCATCCATGGGCGATTTACGCAATGATTGCGCTCGCCATCGCA
TATTCGACGTTTAGAAAAGGTCGTCCCGCGACGATTGGGGAGACAATCGGTTCGCTCGTCCATAACCGCTACGAACAACC
CGTGAAGCAAACGGTCGATATCCTCGCAGTCATTGCGACTGCATTCGGGGTAGCGACTTCACTTGGTTTTGGTGCTCAGC
AAATTGCCGGTGGCCTTCATTACCTCATCCCTGGGGTACCGAACGCTTTCGTGACACAGTTGATCATCATTGCCGTCGTA
ACGGTGCTCTACATGATCAGTGCTTCGACAGGTCTTGACAAAGGAATTCGAATCTTAAGTAATACGAATATATTCTTGGC
AATTGTCCTACTTGTAGCGACGATGTTTGCGGGACCGACAGCTTTCATTTTCGACTTGTTCACACAAACAGTCGGAACTT
ATTTGCAGCAGCTCCCGAGCATGAGTTTCCGTACAGCCGCATTTGAACCCGTTGAACGTGAATGGGTGAACGGTTGGACC
ATCTTCTACTGGGCTTGGTGGATTTCTTGGTCACCATTCGTCGGAACGTTTATCGCACGTGTATCGAAAGGGCGTACGAT
TCGGGAATTTATCATCGGAATCTTACTTGTCCCGACATCATTCGGATTATTATGGTTCTCCGTCTTCGGCGGTTCGGCCA
TCTGGGCTGATTTGTTCGGAGGATATAGCTTAATTGATCAAGTAAACGAAATCGGTACTGAAGTCGGGCTGTTCGCACTG
TTTGAAACGTTCGGTGGTTTTGGGACGCTTCTCAGTGTAATCGCCATCTTCTTGATTTCGACATTCTTTATTACATCGGC
CGACTCTGCGACATACGTCCTCGGTATGCTTACATCGAACGGGAAGCTTATCCCGCCGATGGGGATTAAAATGACTTGGG
GAATCATCCAATCATCAATCGCAGCCGTATTGTTGTATGCTGGTGGATTAGATGCTCTACAAGCTGTTGCGATTCTCGCA
GCCTTCCCATTCATCTTCGTGCTCATCTTTATGATGATTGCCTTATTCAAGGATCTTACCGACGAACCGGATGAACGAGA
TAAATGGCTAGAGATGCAAAAGAAAGATGAAGATAAACTAGGTTAA

Upstream 100 bases:

>100_bases
AAACAACACATTTATGTACACATAGGGTTGTGACTTGTTTCAAAAGTATGTTAGGGTGGAAAACGTATATGAAAATAGAA
AATTAAATAGGAGTGATTTT

Downstream 100 bases:

>100_bases
TTCGAAAGGGTCTCAGATGAGGCCCTTTTTCGGTCAAAAATATTTTGCTTGTCCTTCTCGCTAGTGTAGGATAGTAAGTG
AAGAAACGAGGAGGATAGGC

Product: choline/carnitine/betaine transporter

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 521; Mature: 521

Protein sequence:

>521_residues
MERKSRVTTVFVVSAIITGLFTIWGLFPESLLGNASLLNVTSTLQGWLSNGMGWFYLLSATGFLLVAIFLIFSRYGSIRL
GKDTDRPEFSYLSWFAMLFSAGMGIGLIFWGAAEPLLHFHSPPFASPTPEGDARTAMRYAFFHWGLHPWAIYAMIALAIA
YSTFRKGRPATIGETIGSLVHNRYEQPVKQTVDILAVIATAFGVATSLGFGAQQIAGGLHYLIPGVPNAFVTQLIIIAVV
TVLYMISASTGLDKGIRILSNTNIFLAIVLLVATMFAGPTAFIFDLFTQTVGTYLQQLPSMSFRTAAFEPVEREWVNGWT
IFYWAWWISWSPFVGTFIARVSKGRTIREFIIGILLVPTSFGLLWFSVFGGSAIWADLFGGYSLIDQVNEIGTEVGLFAL
FETFGGFGTLLSVIAIFLISTFFITSADSATYVLGMLTSNGKLIPPMGIKMTWGIIQSSIAAVLLYAGGLDALQAVAILA
AFPFIFVLIFMMIALFKDLTDEPDERDKWLEMQKKDEDKLG

Sequences:

>Translated_521_residues
MERKSRVTTVFVVSAIITGLFTIWGLFPESLLGNASLLNVTSTLQGWLSNGMGWFYLLSATGFLLVAIFLIFSRYGSIRL
GKDTDRPEFSYLSWFAMLFSAGMGIGLIFWGAAEPLLHFHSPPFASPTPEGDARTAMRYAFFHWGLHPWAIYAMIALAIA
YSTFRKGRPATIGETIGSLVHNRYEQPVKQTVDILAVIATAFGVATSLGFGAQQIAGGLHYLIPGVPNAFVTQLIIIAVV
TVLYMISASTGLDKGIRILSNTNIFLAIVLLVATMFAGPTAFIFDLFTQTVGTYLQQLPSMSFRTAAFEPVEREWVNGWT
IFYWAWWISWSPFVGTFIARVSKGRTIREFIIGILLVPTSFGLLWFSVFGGSAIWADLFGGYSLIDQVNEIGTEVGLFAL
FETFGGFGTLLSVIAIFLISTFFITSADSATYVLGMLTSNGKLIPPMGIKMTWGIIQSSIAAVLLYAGGLDALQAVAILA
AFPFIFVLIFMMIALFKDLTDEPDERDKWLEMQKKDEDKLG
>Mature_521_residues
MERKSRVTTVFVVSAIITGLFTIWGLFPESLLGNASLLNVTSTLQGWLSNGMGWFYLLSATGFLLVAIFLIFSRYGSIRL
GKDTDRPEFSYLSWFAMLFSAGMGIGLIFWGAAEPLLHFHSPPFASPTPEGDARTAMRYAFFHWGLHPWAIYAMIALAIA
YSTFRKGRPATIGETIGSLVHNRYEQPVKQTVDILAVIATAFGVATSLGFGAQQIAGGLHYLIPGVPNAFVTQLIIIAVV
TVLYMISASTGLDKGIRILSNTNIFLAIVLLVATMFAGPTAFIFDLFTQTVGTYLQQLPSMSFRTAAFEPVEREWVNGWT
IFYWAWWISWSPFVGTFIARVSKGRTIREFIIGILLVPTSFGLLWFSVFGGSAIWADLFGGYSLIDQVNEIGTEVGLFAL
FETFGGFGTLLSVIAIFLISTFFITSADSATYVLGMLTSNGKLIPPMGIKMTWGIIQSSIAAVLLYAGGLDALQAVAILA
AFPFIFVLIFMMIALFKDLTDEPDERDKWLEMQKKDEDKLG

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=511, Percent_Identity=37.7690802348337, Blast_Score=342, Evalue=3e-95,
Organism=Escherichia coli, GI1786224, Length=469, Percent_Identity=30.4904051172708, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI1788102, Length=453, Percent_Identity=28.6975717439294, Blast_Score=181, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 57297; Mature: 57297

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS01303 BCCT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]