BacMap

Definition	Exiguobacterium sp. AT1b, complete genome.
Accession	NC_012673
Length	2,999,895

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The map label for this gene is fabG [C]

Identifier: 229916091

GI number: 229916091

Start: 322245

End: 322952

Strand: Reverse

Name: fabG [C]

Synonym: EAT1b_0360

Alternate gene names: 229916091

Gene position: 322952-322245 (Counterclockwise)

Preceding gene: 229916092

Following gene: 229916090

Centisome position: 10.77

GC content: 44.77

Gene sequence:

>708_bases
ATGATGAAAGACGTGATGATTGTAACAGGGGTCAGTCAAGGAATAGGTTACGCGATTGCACAAGCTTTCGTAAATCAGTA
TGAAGTGATCGGGTTAGATATTGGAGATGACCCGGACATTAAAGGGTTACGCTTTTATCAAGTAGACCTTGGAGACACGT
CTGAACTTAAGCGTGTCTTTGACGATATTCACAACGAATTTGGTCGAGCCCATGTGTTGATCAACAATGGAGGAATCGCT
TCGCTGGTCAAGCCGATTGATGAGCTTGAGATTGACACGTTCAAACGAGTCATCGATGTCAATTTAGTCGGGACATTTAG
TTGTGCGAAATACTTTTTAGAACTGAATCAAGATGAGTCATATGGAAGAATCGTTAACATGGCCTCGACACGTGCTTTCC
AGAACGAACCGCATTGGGATGCATATGGTGCATCTAAAGGAGGCATCGTCGGCTTGACCCATTCACTTGCCGTCTCATTA
GGCGACCGCCCTGTGACGGTGAATGCAATCAGCCCAGGATGGATTCATACGTCTGATGAGTCGTTACGTGAGATTGATCA
TGAACAACATCCTTCGGGACGGGTCGGTGAGCCAGAAGATATCGTCCGCGCTGTGAGATACTTGATCGATCGTCAAAACA
GTTTTGTGAACGGGCATAATCATGTCGTAGATGGAGGAATGACGAAAAAAATGATTTATGAAGAATAA

Upstream 100 bases:

>100_bases
AGCGGCACTTGCTGCTGGAGCGAGTGTGATCACGACTTCGAATCGAAAACTATGGTCCGACTAATCGGGATTTGGGTATA
CAGTCTTTTAGGAGGGATGA

Downstream 100 bases:

>100_bases
TGTGACCATATACCTAAATTCGAGATGTTTTTAGGTTATATGTACCTAATTCCGGGTAATGAATAGGAGAGTTTTTATCC
GGAAGAAGGAGGATGGCGAC

Product: short-chain dehydrogenase/reductase SDR

Products: (3R)-hydroxyacyl-[acyl-carrier-protein]; NADP; NADPH; Proton; beta-ketoacyl-ACP [C]

Alternate protein names: NA

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MMKDVMIVTGVSQGIGYAIAQAFVNQYEVIGLDIGDDPDIKGLRFYQVDLGDTSELKRVFDDIHNEFGRAHVLINNGGIA
SLVKPIDELEIDTFKRVIDVNLVGTFSCAKYFLELNQDESYGRIVNMASTRAFQNEPHWDAYGASKGGIVGLTHSLAVSL
GDRPVTVNAISPGWIHTSDESLREIDHEQHPSGRVGEPEDIVRAVRYLIDRQNSFVNGHNHVVDGGMTKKMIYEE

Sequences:

>Translated_235_residues
MMKDVMIVTGVSQGIGYAIAQAFVNQYEVIGLDIGDDPDIKGLRFYQVDLGDTSELKRVFDDIHNEFGRAHVLINNGGIA
SLVKPIDELEIDTFKRVIDVNLVGTFSCAKYFLELNQDESYGRIVNMASTRAFQNEPHWDAYGASKGGIVGLTHSLAVSL
GDRPVTVNAISPGWIHTSDESLREIDHEQHPSGRVGEPEDIVRAVRYLIDRQNSFVNGHNHVVDGGMTKKMIYEE
>Mature_235_residues
MMKDVMIVTGVSQGIGYAIAQAFVNQYEVIGLDIGDDPDIKGLRFYQVDLGDTSELKRVFDDIHNEFGRAHVLINNGGIA
SLVKPIDELEIDTFKRVIDVNLVGTFSCAKYFLELNQDESYGRIVNMASTRAFQNEPHWDAYGASKGGIVGLTHSLAVSL
GDRPVTVNAISPGWIHTSDESLREIDHEQHPSGRVGEPEDIVRAVRYLIDRQNSFVNGHNHVVDGGMTKKMIYEE

Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI4758504, Length=248, Percent_Identity=31.0483870967742, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI40254992, Length=239, Percent_Identity=28.4518828451883, Blast_Score=92, Evalue=3e-19,
Organism=Homo sapiens, GI32483357, Length=252, Percent_Identity=24.2063492063492, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI19923817, Length=255, Percent_Identity=27.0588235294118, Blast_Score=84, Evalue=8e-17,
Organism=Homo sapiens, GI83715985, Length=243, Percent_Identity=29.6296296296296, Blast_Score=84, Evalue=9e-17,
Organism=Homo sapiens, GI31542939, Length=222, Percent_Identity=28.3783783783784, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI10190704, Length=248, Percent_Identity=27.0161290322581, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI59889578, Length=200, Percent_Identity=27, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI15277342, Length=252, Percent_Identity=24.6031746031746, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI7705925, Length=236, Percent_Identity=26.6949152542373, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI126723750, Length=252, Percent_Identity=23.015873015873, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI5031737, Length=250, Percent_Identity=24, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1787335, Length=244, Percent_Identity=28.6885245901639, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI1789208, Length=243, Percent_Identity=28.3950617283951, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI87082160, Length=239, Percent_Identity=28.4518828451883, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788459, Length=247, Percent_Identity=29.5546558704453, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI87082100, Length=248, Percent_Identity=30.241935483871, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1790717, Length=241, Percent_Identity=26.5560165975104, Blast_Score=88, Evalue=4e-19,
Organism=Escherichia coli, GI1786812, Length=237, Percent_Identity=27.4261603375527, Blast_Score=81, Evalue=5e-17,
Organism=Escherichia coli, GI2367175, Length=236, Percent_Identity=26.271186440678, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1789057, Length=259, Percent_Identity=27.027027027027, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789378, Length=244, Percent_Identity=23.7704918032787, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787905, Length=244, Percent_Identity=27.8688524590164, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI2367365, Length=233, Percent_Identity=27.8969957081545, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17561402, Length=249, Percent_Identity=27.710843373494, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17538182, Length=246, Percent_Identity=26.8292682926829, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17560676, Length=245, Percent_Identity=24.8979591836735, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI25147288, Length=245, Percent_Identity=28.5714285714286, Blast_Score=80, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17536651, Length=245, Percent_Identity=26.530612244898, Blast_Score=77, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17562910, Length=204, Percent_Identity=30.3921568627451, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17555706, Length=240, Percent_Identity=29.5833333333333, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71994604, Length=247, Percent_Identity=26.3157894736842, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17559104, Length=263, Percent_Identity=26.2357414448669, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17560220, Length=261, Percent_Identity=26.4367816091954, Blast_Score=70, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI72000259, Length=258, Percent_Identity=27.906976744186, Blast_Score=68, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI115534694, Length=239, Percent_Identity=27.1966527196653, Blast_Score=67, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17563726, Length=263, Percent_Identity=26.2357414448669, Blast_Score=67, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17551412, Length=269, Percent_Identity=26.3940520446097, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17536025, Length=245, Percent_Identity=24.8979591836735, Blast_Score=67, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17538480, Length=268, Percent_Identity=25, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17562906, Length=187, Percent_Identity=27.807486631016, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324126, Length=193, Percent_Identity=29.5336787564767, Blast_Score=80, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6322861, Length=232, Percent_Identity=27.5862068965517, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320089, Length=185, Percent_Identity=27.027027027027, Blast_Score=63, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6322226, Length=216, Percent_Identity=26.8518518518519, Blast_Score=62, Evalue=1e-10,
Organism=Drosophila melanogaster, GI17737361, Length=255, Percent_Identity=29.4117647058824, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21355319, Length=245, Percent_Identity=24.8979591836735, Blast_Score=91, Evalue=5e-19,
Organism=Drosophila melanogaster, GI23397609, Length=244, Percent_Identity=29.0983606557377, Blast_Score=91, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24643142, Length=244, Percent_Identity=29.5081967213115, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24644339, Length=248, Percent_Identity=25.8064516129032, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24644337, Length=170, Percent_Identity=32.3529411764706, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24639444, Length=243, Percent_Identity=26.7489711934156, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21357041, Length=249, Percent_Identity=25.7028112449799, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI28571526, Length=248, Percent_Identity=27.4193548387097, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI116007236, Length=229, Percent_Identity=27.0742358078603, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100 [C]

Molecular weight: Translated: 26092; Mature: 26092

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKDVMIVTGVSQGIGYAIAQAFVNQYEVIGLDIGDDPDIKGLRFYQVDLGDTSELKRVF
CCCCEEEEECCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCEEEEEEECCCHHHHHHHH
DDIHNEFGRAHVLINNGGIASLVKPIDELEIDTFKRVIDVNLVGTFSCAKYFLELNQDES
HHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHCCCCCC
YGRIVNMASTRAFQNEPHWDAYGASKGGIVGLTHSLAVSLGDRPVTVNAISPGWIHTSDE
CHHEEHHHHHHHHCCCCCCCCCCCCCCCEEEEHHHHEEECCCCCEEEEECCCCEEECCHH
SLREIDHEQHPSGRVGEPEDIVRAVRYLIDRQNSFVNGHNHVVDGGMTKKMIYEE
HHHHCCHHCCCCCCCCCHHHHHHHHHHHHHCCCCEECCCCEEECCCCCCHHCCCC
>Mature Secondary Structure
MMKDVMIVTGVSQGIGYAIAQAFVNQYEVIGLDIGDDPDIKGLRFYQVDLGDTSELKRVF
CCCCEEEEECCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCEEEEEEECCCHHHHHHHH
DDIHNEFGRAHVLINNGGIASLVKPIDELEIDTFKRVIDVNLVGTFSCAKYFLELNQDES
HHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHCCCCCC
YGRIVNMASTRAFQNEPHWDAYGASKGGIVGLTHSLAVSLGDRPVTVNAISPGWIHTSDE
CHHEEHHHHHHHHCCCCCCCCCCCCCCCEEEEHHHHEEECCCCCEEEEECCCCEEECCHH
SLREIDHEQHPSGRVGEPEDIVRAVRYLIDRQNSFVNGHNHVVDGGMTKKMIYEE
HHHHCCHHCCCCCCCCCHHHHHHHHHHHHHCCCCEECCCCEEECCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Oxoacyl-[acyl-carrier-protein]; NADPH; D-3-hydroxy-acyl-ACP; NADP [C]

Specific reaction: Oxoacyl-[acyl-carrier-protein] + NADPH = (3R)-hydroxyacyl-[acyl-carrier-protein] + NADP+ D-3-hydroxy-acyl-ACP + NADP = NADPH + Proton + beta-ketoacyl-ACP [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550425; 10360571 [H]