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The map label for this gene is opuE [H]

Identifier: 229916044

GI number: 229916044

Start: 280902

End: 282383

Strand: Direct

Name: opuE [H]

Synonym: EAT1b_0312

Alternate gene names: 229916044

Gene position: 280902-282383 (Clockwise)

Preceding gene: 229916043

Following gene: 229916052

Centisome position: 9.36

GC content: 47.98

Gene sequence:

>1482_bases
ATGCTATGGCAAGAATGGGTATCCATCGCGCTTTATCTCGTGATGATGCTTCTTATCGGATACGTTGCGTATAAGCGAAC
GACCGATTCATCAGATTATATGTTAGGTGGCCGTAAAATGGGGCCAGGCGTTACAGCCATGTCAGCCGGAGCTTCGGATA
TGAGTGGTTGGATGTTAATGGGGTTACCGGGTGCAATGTATGCAACTGGACTATCAGCAATTTGGTTAGCCGTTGGATTG
GTCATCGGAGCTTACTTGAACTACTTGTTAGTTGCTCCACGACTTCGCGTCTTCACAGAGATGGCGAACGATTCGATTAC
GATTCCAGACTTCTTAGAGAATCGGTTCCGAGATCACTCGAACTGGCTACGGATCCTATCTGCATCCGTCATCATCATCT
TCTTCACGTTCTACACGTCAGCCGGACTTGTATCTGGCGGGAAGTTGTTTGAAAGCTCATTCGGATTAAACTATCAGACA
GGTGTTATCGTCACAATTAGTGTCGTGATCTTGTACACCCTCTTCGGTGGGTTCACGGCAGTAAGTTGGACGGACTTTGC
ACAAGGTGTCATCATGTTCTTGGCACTCGTACTTGTACCACTTGTCGCTTTAACAGATATCGGCGGAGTAGACACTGCTG
TCAACTCTGCGACGACAATCAATGCTGATTTGTTTGATGTCTTCAAAGGGACGTCAACACTTGGGATCATCGGTCTTCTT
GCATGGGGACTTGGTTACTTCGGTCAACCACACATCATCGTTCGCTTCATGGCAATCCGTGATGTGAAATCATTGAAAAC
GGCTCGTCGAATCGGGATGAGCTGGATGACCGTGTCAATCATCGGTGCAGTCATGACTGGTTTCGTCGGAATCGCGTATT
TCCGCGGACAAGGAAATCCACTCGGGGACCCGGAAACAGTCTTCATTCGATTCTCTGAAGTCTTGTTCCACCCGTTCATC
ACTGGATTCTTGTTAGCCGCAATTCTTGCAGCCGTCATGTCTACCGTATCATCACAGTTGCTCGTCACGTCATCTGCGTT
GACAGAAGACTTCTATCGTAAGTTCTTCAACAAAGATGCAAGTGAGAAGACGATGGTCCTTGCCGGACGTGTTGGTGTCT
TGCTTGTCGGAGTTGTAGCGACGCTCATGTCACTCAATCCATCAAGCACAATCCTCACACTCGTCGGATATGCATGGGCT
GGATTCGGTTCTGCATTTGGACCAGCCATCCTCTTGTCACTTTACTGGAGCAAGATGACACGTCAAGGTGCCCTCGCTGG
TATCCTCGTCGGTGCCATCACCGTCATTGTCTGGATCGTCACTGGACTTTCGACAGTCCTTTATGAGATGGTCCCTGGCT
TCTTCCTAAGTATGTTGGCAATCGTACTTGTCAGTAACTTTACTAGCCAGAAAGAACAAAAACGTATCAACCGTGAATTC
GGTGAAATGGAAGCTGAATTGGCAGCTGCGAAAAAAGAGTAA

Upstream 100 bases:

>100_bases
AGTTCGCATTTTCAACTTTTGTTTTTAACTGTGTGATGTCTTCACACAATCGCGAACGAGTTGATCAAATAGATCTTTTA
ACTTAATAGGAGGTTATTCA

Downstream 100 bases:

>100_bases
CGATTCACACAAAAACTCGGTTCCTGATCAAGGGACCGAGTTTTTGCTTTACATCGATGCTGCAATTAACCAAACACGAA
TCACGAACGGTACGAGAACC

Product: sodium/proline symporter

Products: NA

Alternate protein names: Sodium/proline symporter [H]

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MLWQEWVSIALYLVMMLLIGYVAYKRTTDSSDYMLGGRKMGPGVTAMSAGASDMSGWMLMGLPGAMYATGLSAIWLAVGL
VIGAYLNYLLVAPRLRVFTEMANDSITIPDFLENRFRDHSNWLRILSASVIIIFFTFYTSAGLVSGGKLFESSFGLNYQT
GVIVTISVVILYTLFGGFTAVSWTDFAQGVIMFLALVLVPLVALTDIGGVDTAVNSATTINADLFDVFKGTSTLGIIGLL
AWGLGYFGQPHIIVRFMAIRDVKSLKTARRIGMSWMTVSIIGAVMTGFVGIAYFRGQGNPLGDPETVFIRFSEVLFHPFI
TGFLLAAILAAVMSTVSSQLLVTSSALTEDFYRKFFNKDASEKTMVLAGRVGVLLVGVVATLMSLNPSSTILTLVGYAWA
GFGSAFGPAILLSLYWSKMTRQGALAGILVGAITVIVWIVTGLSTVLYEMVPGFFLSMLAIVLVSNFTSQKEQKRINREF
GEMEAELAAAKKE

Sequences:

>Translated_493_residues
MLWQEWVSIALYLVMMLLIGYVAYKRTTDSSDYMLGGRKMGPGVTAMSAGASDMSGWMLMGLPGAMYATGLSAIWLAVGL
VIGAYLNYLLVAPRLRVFTEMANDSITIPDFLENRFRDHSNWLRILSASVIIIFFTFYTSAGLVSGGKLFESSFGLNYQT
GVIVTISVVILYTLFGGFTAVSWTDFAQGVIMFLALVLVPLVALTDIGGVDTAVNSATTINADLFDVFKGTSTLGIIGLL
AWGLGYFGQPHIIVRFMAIRDVKSLKTARRIGMSWMTVSIIGAVMTGFVGIAYFRGQGNPLGDPETVFIRFSEVLFHPFI
TGFLLAAILAAVMSTVSSQLLVTSSALTEDFYRKFFNKDASEKTMVLAGRVGVLLVGVVATLMSLNPSSTILTLVGYAWA
GFGSAFGPAILLSLYWSKMTRQGALAGILVGAITVIVWIVTGLSTVLYEMVPGFFLSMLAIVLVSNFTSQKEQKRINREF
GEMEAELAAAKKE
>Mature_493_residues
MLWQEWVSIALYLVMMLLIGYVAYKRTTDSSDYMLGGRKMGPGVTAMSAGASDMSGWMLMGLPGAMYATGLSAIWLAVGL
VIGAYLNYLLVAPRLRVFTEMANDSITIPDFLENRFRDHSNWLRILSASVIIIFFTFYTSAGLVSGGKLFESSFGLNYQT
GVIVTISVVILYTLFGGFTAVSWTDFAQGVIMFLALVLVPLVALTDIGGVDTAVNSATTINADLFDVFKGTSTLGIIGLL
AWGLGYFGQPHIIVRFMAIRDVKSLKTARRIGMSWMTVSIIGAVMTGFVGIAYFRGQGNPLGDPETVFIRFSEVLFHPFI
TGFLLAAILAAVMSTVSSQLLVTSSALTEDFYRKFFNKDASEKTMVLAGRVGVLLVGVVATLMSLNPSSTILTLVGYAWA
GFGSAFGPAILLSLYWSKMTRQGALAGILVGAITVIVWIVTGLSTVLYEMVPGFFLSMLAIVLVSNFTSQKEQKRINREF
GEMEAELAAAKKE

Specific function: Catalyzes the sodium-dependent uptake of extracellular proline [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI310128183, Length=493, Percent_Identity=54.158215010142, Blast_Score=535, Evalue=1e-152,
Organism=Homo sapiens, GI14140236, Length=474, Percent_Identity=24.2616033755274, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI157671931, Length=445, Percent_Identity=24.7191011235955, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI109659836, Length=384, Percent_Identity=24.4791666666667, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI110835708, Length=487, Percent_Identity=22.7926078028747, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI4507031, Length=364, Percent_Identity=24.1758241758242, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI4507035, Length=353, Percent_Identity=24.0793201133144, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI109659839, Length=400, Percent_Identity=23.5, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1787251, Length=491, Percent_Identity=48.8798370672098, Blast_Score=466, Evalue=1e-132,
Organism=Escherichia coli, GI1790503, Length=454, Percent_Identity=26.8722466960352, Blast_Score=153, Evalue=3e-38,
Organism=Escherichia coli, GI87082237, Length=433, Percent_Identity=28.4064665127021, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1790113, Length=391, Percent_Identity=25.3196930946292, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17539284, Length=458, Percent_Identity=24.4541484716157, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI115533094, Length=382, Percent_Identity=24.869109947644, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24645928, Length=381, Percent_Identity=25.7217847769029, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24640370, Length=353, Percent_Identity=26.3456090651558, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI221459584, Length=374, Percent_Identity=23.2620320855615, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24651739, Length=465, Percent_Identity=24.0860215053763, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24651741, Length=260, Percent_Identity=27.3076923076923, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI221459588, Length=384, Percent_Identity=23.1770833333333, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI281362918, Length=197, Percent_Identity=27.4111675126904, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI221459586, Length=384, Percent_Identity=23.6979166666667, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI221459582, Length=389, Percent_Identity=23.9074550128535, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI28573698, Length=469, Percent_Identity=25.5863539445629, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 53397; Mature: 53397

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: PS00456 NA_SOLUT_SYMP_1 ; PS00457 NA_SOLUT_SYMP_2 ; PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHCCC
>Mature Secondary Structure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HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969499; 9384377 [H]