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The map label for this gene is dacF [H]

Identifier: 229916043

GI number: 229916043

Start: 279543

End: 280556

Strand: Direct

Name: dacF [H]

Synonym: EAT1b_0311

Alternate gene names: 229916043

Gene position: 279543-280556 (Clockwise)

Preceding gene: 229916037

Following gene: 229916044

Centisome position: 9.32

GC content: 47.83

Gene sequence:

>1014_bases
TTGTTGACCCGTAGAAGGAGACACCGAAGAAGAACGTACATCATGCGAAGAATGATTGCCGCAGTCGTGTTGCTCGGAAT
CATCGCACTGATCCCGACGTTGGTCGGATTTAAACAAGATAAAGAGGCAATGATACCAGCAGAGGCTTCCACGCCAAAGG
TCGAACCGATGAAGCAAGGCCCGGTTTTTCCGGAGATCGATATGGGTTCATGGCGGCCGAGTGAGGCGACATTGCTTTCA
AATGAAGAAATCCCTGTCGGCGCGTTGACCTTCGTCAATGTGACCAACCAAGAGATATTAATCGATAAAAATGCCAATGA
CCGTGTATTTCCAGCCAGTACGACCAAATTGATGACAGCACTTCTCGCCTATGAAAAGGCGTCTGCAGCGAATGAGCTCG
ACCAGCCGATCATCACGATCCAACAGTCGACACTCGACATTCCATGGGACAGCCACATCGCACATCTTGCCATCGGAGAT
ACATTGACAGTCCGGCAAGCGCTTTACGCCACGCTACTCGAATCTGGAAACGATGCTGCGAACAGCCTTGCAGAATACAC
GAGCGGGTCGACAAATGCATTCGTTCAATTGATGAATGACCGAGCAAAGGTTTTAGGATTGACTGGAACGAACTTCGTAA
ATGCCCATGGCTACTCGGATCCGAATCATTATACAACGGCGAACGACATGGCTAAAATCGCCTTCGCATTCAGTACATAC
CCGGAGTTGATTGAAATTGCCGGGACTTATGAATTTAAAGCCGAATTCAAAGATAAAGACGGTAACGGCAAGCGTCGTTG
GTGGTATCACCTCGGTTCATCCGTCAATGAACGAAGTGTCAATTACTCGAAGTTCGTGACCGCCACGAAGACGGGTTATA
CGGACGAATCCGGATATACGATGGTATTCATCATGAAACATAATAGCGAAACATATATCTTGGTTACGATGCAGGCGAAG
TCTGGACAAACGTTCCCGACGATGCGACTCGTTGAAGAAAAAGCATTCAAATAA

Upstream 100 bases:

>100_bases
TTCACCGCCGATCAATCATTGACCTAGTTCTTTATTTTATCAGCGAAAAAAATAAGCGTTTTCTATTATGTTTATAGAGT
AACCAAAAAAGGAGGGACTT

Downstream 100 bases:

>100_bases
GGAACTGAAGAGCATTTAAAGATGCTCTTTTTCTATTGATAAAAAATATGTTTTCTGAAAATTTTCCCATAAATATTACA
TTCGTGTTACACAGAAAAGT

Product: peptidase S11 D-alanyl-D-alanine carboxypeptidase 1

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MLTRRRRHRRRTYIMRRMIAAVVLLGIIALIPTLVGFKQDKEAMIPAEASTPKVEPMKQGPVFPEIDMGSWRPSEATLLS
NEEIPVGALTFVNVTNQEILIDKNANDRVFPASTTKLMTALLAYEKASAANELDQPIITIQQSTLDIPWDSHIAHLAIGD
TLTVRQALYATLLESGNDAANSLAEYTSGSTNAFVQLMNDRAKVLGLTGTNFVNAHGYSDPNHYTTANDMAKIAFAFSTY
PELIEIAGTYEFKAEFKDKDGNGKRRWWYHLGSSVNERSVNYSKFVTATKTGYTDESGYTMVFIMKHNSETYILVTMQAK
SGQTFPTMRLVEEKAFK

Sequences:

>Translated_337_residues
MLTRRRRHRRRTYIMRRMIAAVVLLGIIALIPTLVGFKQDKEAMIPAEASTPKVEPMKQGPVFPEIDMGSWRPSEATLLS
NEEIPVGALTFVNVTNQEILIDKNANDRVFPASTTKLMTALLAYEKASAANELDQPIITIQQSTLDIPWDSHIAHLAIGD
TLTVRQALYATLLESGNDAANSLAEYTSGSTNAFVQLMNDRAKVLGLTGTNFVNAHGYSDPNHYTTANDMAKIAFAFSTY
PELIEIAGTYEFKAEFKDKDGNGKRRWWYHLGSSVNERSVNYSKFVTATKTGYTDESGYTMVFIMKHNSETYILVTMQAK
SGQTFPTMRLVEEKAFK
>Mature_337_residues
MLTRRRRHRRRTYIMRRMIAAVVLLGIIALIPTLVGFKQDKEAMIPAEASTPKVEPMKQGPVFPEIDMGSWRPSEATLLS
NEEIPVGALTFVNVTNQEILIDKNANDRVFPASTTKLMTALLAYEKASAANELDQPIITIQQSTLDIPWDSHIAHLAIGD
TLTVRQALYATLLESGNDAANSLAEYTSGSTNAFVQLMNDRAKVLGLTGTNFVNAHGYSDPNHYTTANDMAKIAFAFSTY
PELIEIAGTYEFKAEFKDKDGNGKRRWWYHLGSSVNERSVNYSKFVTATKTGYTDESGYTMVFIMKHNSETYILVTMQAK
SGQTFPTMRLVEEKAFK

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=243, Percent_Identity=30.0411522633745, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1786851, Length=228, Percent_Identity=29.3859649122807, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI87082022, Length=247, Percent_Identity=27.9352226720648, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI87082054, Length=242, Percent_Identity=30.1652892561983, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 37656; Mature: 37656

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRRRRHRRRTYIMRRMIAAVVLLGIIALIPTLVGFKQDKEAMIPAEASTPKVEPMKQG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHCC
PVFPEIDMGSWRPSEATLLSNEEIPVGALTFVNVTNQEILIDKNANDRVFPASTTKLMTA
CCCCCCCCCCCCCCCCEEECCCCCCEEEEEEEEECCCEEEEECCCCCCCCCCHHHHHHHH
LLAYEKASAANELDQPIITIQQSTLDIPWDSHIAHLAIGDTLTVRQALYATLLESGNDAA
HHHHHHHHHHHHCCCCEEEEECCEECCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCHHH
NSLAEYTSGSTNAFVQLMNDRAKVLGLTGTNFVNAHGYSDPNHYTTANDMAKIAFAFSTY
HHHHHHCCCCHHHHHHHHCCCEEEEEECCCCEEECCCCCCCCCCCCHHHHHHHHHHHHCC
PELIEIAGTYEFKAEFKDKDGNGKRRWWYHLGSSVNERSVNYSKFVTATKTGYTDESGYT
HHHHHHHCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCHHHEEEEECCCCCCCCCCE
MVFIMKHNSETYILVTMQAKSGQTFPTMRLVEEKAFK
EEEEEEECCCEEEEEEEECCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MLTRRRRHRRRTYIMRRMIAAVVLLGIIALIPTLVGFKQDKEAMIPAEASTPKVEPMKQG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHCC
PVFPEIDMGSWRPSEATLLSNEEIPVGALTFVNVTNQEILIDKNANDRVFPASTTKLMTA
CCCCCCCCCCCCCCCCEEECCCCCCEEEEEEEEECCCEEEEECCCCCCCCCCHHHHHHHH
LLAYEKASAANELDQPIITIQQSTLDIPWDSHIAHLAIGDTLTVRQALYATLLESGNDAA
HHHHHHHHHHHHCCCCEEEEECCEECCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCHHH
NSLAEYTSGSTNAFVQLMNDRAKVLGLTGTNFVNAHGYSDPNHYTTANDMAKIAFAFSTY
HHHHHHCCCCHHHHHHHHCCCEEEEEECCCCEEECCCCCCCCCCCCHHHHHHHHHHHHCC
PELIEIAGTYEFKAEFKDKDGNGKRRWWYHLGSSVNERSVNYSKFVTATKTGYTDESGYT
HHHHHHHCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCHHHEEEEECCCCCCCCCCE
MVFIMKHNSETYILVTMQAKSGQTFPTMRLVEEKAFK
EEEEEEECCCEEEEEEEECCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]