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The map label for this gene is patB [H]

Identifier: 229916016

GI number: 229916016

Start: 251539

End: 252711

Strand: Reverse

Name: patB [H]

Synonym: EAT1b_0284

Alternate gene names: 229916016

Gene position: 252711-251539 (Counterclockwise)

Preceding gene: 229916018

Following gene: 229916015

Centisome position: 8.42

GC content: 51.41

Gene sequence:

>1173_bases
ATGAAATCAATTTTACAACAACAACTTGTACGTAAAGGTACGAATTCGGTGAAATGGGATGGGCTGGAACGATGGTTCCG
TTTATCTGAGGATGTGTTACCGCTTTGGGTAGCGGATATCGATATCCGGACTGCCCCGTCCATCTCCGAGGCGCTCGCCG
CAAGAGCCGCTCAAGGGGAATTCGGGTATACATTGTTCGCACCTGAAGCGCAGAAGGCGGTCAGTGATTGGTACGGCCGA
CGTCACGATTTGATTGTAGACCCGAACCATGTGTTATTCTCTAGCGGTGTTGTACCTGCGTTGACTCATATCGTCGAGGC
GTTGACTGAAGTCGGAGATGGCGTCGTCATCCAGTCACCTGTCTATCCACCATTCCATGAACTCGTTGTCGGCTCCAAAC
GACAAGTGTTCGATGCCCCGTTGAAAAAAACTGACGAACGCTACGAGATGGATTTTGTCGCACTCGAGGAAGCGATGAAG
CAAGCGAAACTCTTCTTACTCTGTTCCCCACATAATCCAGTTGGACGTGTCTGGACGAGAGCGGAACTAGAGAAGGTCGT
AGCACTCGCGAATCAATATGAGGTGATCCTCGTCGCTGACGAGATTCATGCAGACTTGACGTTCCCGTCTCATCAACATA
CCGCAATCGGCACACTCGACCCACGTGTCATCACGGTGAGTGCGCCATCAAAAGCATTTAACATCGCCGGATTATTTGCG
TCCTACATCATTTGTCCGGTCGATGAGTGGCGGAAGCAAATCACGAAAGTACAGAACAAACATCATGTGCTCCCGACACC
GTTCGCAAACACGGCTATCATCGCCGCGTATACCGATAACCGCGCGGAACGTTGGCTCGACCAGTTGCGAGAAGAATTGG
AGCAACAAATGAATGACGTGATCGATTCCATCGAATCAAACCTCCCGATGCTCCATACGACCCGTCCGGAAGCGAGTTAT
CTGCTTTGGCTCGATTTCTCGGAGTTACCGATGTCTGCCGAGGAGCGGAAGTCATGGCTTCATCATGAGGTTCGGATCTT
CTTGTCACCGGGTGGTCCGTTCGGTCCTCAAGGTGAAGCGTTCGAACGGATGAATATCGGGACTCGTCGTGAACTGTTGG
ACGAGGCGATTCATCGGCTGAGCGCGCAAATGAAGCGAAAAGCGTTTGTGTAA

Upstream 100 bases:

>100_bases
CTTATCTCGATCGAGTATAACGAAGTCGGCAAGAAACTGCTATGCTTTTTTGGCACGCAGTCGAATCAACTTTGATACAG
TAAGAGGAGGAGGGATGAAC

Downstream 100 bases:

>100_bases
AACGAGAATCGGGAAAAATTCACAATGAGATGATGAAAGAAAGTGAGTGGATGGGATGGAGACGATCAACTGGAAGAAAA
AGATGGAGGAACAGTTAAGG

Product: aminotransferase class I and II

Products: NA

Alternate protein names: CBL; Beta-cystathionase; Cysteine lyase [H]

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MKSILQQQLVRKGTNSVKWDGLERWFRLSEDVLPLWVADIDIRTAPSISEALAARAAQGEFGYTLFAPEAQKAVSDWYGR
RHDLIVDPNHVLFSSGVVPALTHIVEALTEVGDGVVIQSPVYPPFHELVVGSKRQVFDAPLKKTDERYEMDFVALEEAMK
QAKLFLLCSPHNPVGRVWTRAELEKVVALANQYEVILVADEIHADLTFPSHQHTAIGTLDPRVITVSAPSKAFNIAGLFA
SYIICPVDEWRKQITKVQNKHHVLPTPFANTAIIAAYTDNRAERWLDQLREELEQQMNDVIDSIESNLPMLHTTRPEASY
LLWLDFSELPMSAEERKSWLHHEVRIFLSPGGPFGPQGEAFERMNIGTRRELLDEAIHRLSAQMKRKAFV

Sequences:

>Translated_390_residues
MKSILQQQLVRKGTNSVKWDGLERWFRLSEDVLPLWVADIDIRTAPSISEALAARAAQGEFGYTLFAPEAQKAVSDWYGR
RHDLIVDPNHVLFSSGVVPALTHIVEALTEVGDGVVIQSPVYPPFHELVVGSKRQVFDAPLKKTDERYEMDFVALEEAMK
QAKLFLLCSPHNPVGRVWTRAELEKVVALANQYEVILVADEIHADLTFPSHQHTAIGTLDPRVITVSAPSKAFNIAGLFA
SYIICPVDEWRKQITKVQNKHHVLPTPFANTAIIAAYTDNRAERWLDQLREELEQQMNDVIDSIESNLPMLHTTRPEASY
LLWLDFSELPMSAEERKSWLHHEVRIFLSPGGPFGPQGEAFERMNIGTRRELLDEAIHRLSAQMKRKAFV
>Mature_390_residues
MKSILQQQLVRKGTNSVKWDGLERWFRLSEDVLPLWVADIDIRTAPSISEALAARAAQGEFGYTLFAPEAQKAVSDWYGR
RHDLIVDPNHVLFSSGVVPALTHIVEALTEVGDGVVIQSPVYPPFHELVVGSKRQVFDAPLKKTDERYEMDFVALEEAMK
QAKLFLLCSPHNPVGRVWTRAELEKVVALANQYEVILVADEIHADLTFPSHQHTAIGTLDPRVITVSAPSKAFNIAGLFA
SYIICPVDEWRKQITKVQNKHHVLPTPFANTAIIAAYTDNRAERWLDQLREELEQQMNDVIDSIESNLPMLHTTRPEASY
LLWLDFSELPMSAEERKSWLHHEVRIFLSPGGPFGPQGEAFERMNIGTRRELLDEAIHRLSAQMKRKAFV

Specific function: Catalyzes the transformation of cystathionine to homocysteine. Also exhibits cysteine desulfhydrase activity in vitro, producing sulfide from cysteine [H]

COG id: COG1168

COG function: function code E; Bifunctional PLP-dependent enzyme with beta-cystathionase and maltose regulon repressor activities

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. MalY/PatB cystathionine beta-lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI56713254, Length=349, Percent_Identity=24.0687679083095, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI56713256, Length=345, Percent_Identity=24.3478260869565, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI95147551, Length=289, Percent_Identity=24.9134948096886, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI169881279, Length=289, Percent_Identity=24.9134948096886, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI169881281, Length=178, Percent_Identity=25.8426966292135, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1787909, Length=378, Percent_Identity=31.2169312169312, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI1788722, Length=370, Percent_Identity=25.6756756756757, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1787710, Length=304, Percent_Identity=24.0131578947368, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1790797, Length=398, Percent_Identity=20.8542713567839, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1786816, Length=353, Percent_Identity=25.2124645892351, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788627, Length=178, Percent_Identity=24.1573033707865, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17567663, Length=348, Percent_Identity=23.2758620689655, Blast_Score=92, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17567369, Length=319, Percent_Identity=24.4514106583072, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI71994476, Length=179, Percent_Identity=25.1396648044693, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71994472, Length=183, Percent_Identity=24.5901639344262, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322401, Length=279, Percent_Identity=24.0143369175627, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI28573069, Length=354, Percent_Identity=26.271186440678, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24646114, Length=354, Percent_Identity=26.271186440678, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI28573067, Length=354, Percent_Identity=26.271186440678, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI28573065, Length=354, Percent_Identity=26.271186440678, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI18859735, Length=335, Percent_Identity=24.1791044776119, Blast_Score=75, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004839
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =4.4.1.8 [H]

Molecular weight: Translated: 44189; Mature: 44189

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSILQQQLVRKGTNSVKWDGLERWFRLSEDVLPLWVADIDIRTAPSISEALAARAAQGE
CHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHCCCC
FGYTLFAPEAQKAVSDWYGRRHDLIVDPNHVLFSSGVVPALTHIVEALTEVGDGVVIQSP
CCEEEECCHHHHHHHHHCCCCCCEEECCCCEEECCCHHHHHHHHHHHHHHCCCCEEEECC
VYPPFHELVVGSKRQVFDAPLKKTDERYEMDFVALEEAMKQAKLFLLCSPHNPVGRVWTR
CCCCHHHHHCCCCHHHHHCHHHCCCHHHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHH
AELEKVVALANQYEVILVADEIHADLTFPSHQHTAIGTLDPRVITVSAPSKAFNIAGLFA
HHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCEEECCCCEEEEEECCCCHHHHHHHHH
SYIICPVDEWRKQITKVQNKHHVLPTPFANTAIIAAYTDNRAERWLDQLREELEQQMNDV
HHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHH
IDSIESNLPMLHTTRPEASYLLWLDFSELPMSAEERKSWLHHEVRIFLSPGGPFGPQGEA
HHHHHHCCCEEEECCCCCCEEEEEEHHHCCCCHHHHHHHHCEEEEEEECCCCCCCCCCCH
FERMNIGTRRELLDEAIHRLSAQMKRKAFV
HHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKSILQQQLVRKGTNSVKWDGLERWFRLSEDVLPLWVADIDIRTAPSISEALAARAAQGE
CHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHCCCC
FGYTLFAPEAQKAVSDWYGRRHDLIVDPNHVLFSSGVVPALTHIVEALTEVGDGVVIQSP
CCEEEECCHHHHHHHHHCCCCCCEEECCCCEEECCCHHHHHHHHHHHHHHCCCCEEEECC
VYPPFHELVVGSKRQVFDAPLKKTDERYEMDFVALEEAMKQAKLFLLCSPHNPVGRVWTR
CCCCHHHHHCCCCHHHHHCHHHCCCHHHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHH
AELEKVVALANQYEVILVADEIHADLTFPSHQHTAIGTLDPRVITVSAPSKAFNIAGLFA
HHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCEEECCCCEEEEEECCCCHHHHHHHHH
SYIICPVDEWRKQITKVQNKHHVLPTPFANTAIIAAYTDNRAERWLDQLREELEQQMNDV
HHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHH
IDSIESNLPMLHTTRPEASYLLWLDFSELPMSAEERKSWLHHEVRIFLSPGGPFGPQGEA
HHHHHHCCCEEEECCCCCCEEEEEEHHHCCCCHHHHHHHHCEEEEEEECCCCCCCCCCCH
FERMNIGTRRELLDEAIHRLSAQMKRKAFV
HHHHCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8497199; 9274030; 9384377 [H]