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The map label for this gene is yckB [H]

Identifier: 229916003

GI number: 229916003

Start: 237553

End: 238401

Strand: Reverse

Name: yckB [H]

Synonym: EAT1b_0271

Alternate gene names: 229916003

Gene position: 238401-237553 (Counterclockwise)

Preceding gene: 229916005

Following gene: 229916002

Centisome position: 7.95

GC content: 47.7

Gene sequence:

>849_bases
GTGAAACGAACGACTAAAGGTTTGGCAGCAACTTTATTTTTAGCAATCCCACTTGCCGCTTGTGGTGATGATAGCACGGA
TCAAGCAGATACGACACGCTGGGAAGAGATTCAAGAAGAAGGCACATTGACGGTCGGTACGGCCGGTACCCTCTACCCCG
CTTCTTTCCGTGAAGAAGAGAGTGACACGTTGACAGGATTTGATGTCGAGCTCATGAAAGAAATCGCTAAGCGTCTTGAC
CTCGAGATCGAATTCAAAGAAATGGCATTTGACAACATGTTGACGAGTGTGCAGAACGGTCAAATCGACGTGGCAGCGAA
TGATATCTCGGTAACAGAAGACCGTAAAGAGAAGTTTGCCTTCTCAAAACCGTATAAATACACGTACGGGACGGCCATCG
TTCGCAAGAGCGACCTCTCTGGAATCGAATCACTCGAGGACTTAAAAGGTAAGAAAGCCGCTGGTGAGGCGACGACTGTC
TTCATGGACGTGGCTCGAGAATATGGTGCAGAAGAAGTCATCTATGACAACGCGACGAACGATCAATACTTGCGCGACGT
TTCAACAGGACGGACCGATGTCATCTTGAATGACTATTACCTCCAAACGTTGGCCCTTGCCTTCTTCCCTGAGTTTGATA
TCACGATTCACCCGGACATCGCCTATAACCCACAAGAAGTCGCTTTCTTGATGGACAAAGAAAATGACGAACTCCAGGAG
AATATCGACCGTGTACTAGATGAGATGCTCGAAGATGGGACGGTCAAGGAACTATCTGAGCAATTCTACAATGGCGCGGA
TGTTTCGGTAGAATCAGACGTAGACGCAACAATCGTCGAAACGAAGTAA

Upstream 100 bases:

>100_bases
TCAAATTTTAATTTCACCTGTTTAATACATGCTTCACAGTTGCTAGACCGCTGAAGTCATGCTATTTTTAGACAGATGAA
CCATCTAAGGAGGAATTCTT

Downstream 100 bases:

>100_bases
TGACGGCATGACGTTCTCAGAAATTAAATGGGACGCCATCTTTGACGTAGACCTGGCGATTCAATCATTTCCTTATCTAT
TGGGTGGATTACCGAATACA

Product: extracellular solute-binding protein family 3

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MKRTTKGLAATLFLAIPLAACGDDSTDQADTTRWEEIQEEGTLTVGTAGTLYPASFREEESDTLTGFDVELMKEIAKRLD
LEIEFKEMAFDNMLTSVQNGQIDVAANDISVTEDRKEKFAFSKPYKYTYGTAIVRKSDLSGIESLEDLKGKKAAGEATTV
FMDVAREYGAEEVIYDNATNDQYLRDVSTGRTDVILNDYYLQTLALAFFPEFDITIHPDIAYNPQEVAFLMDKENDELQE
NIDRVLDEMLEDGTVKELSEQFYNGADVSVESDVDATIVETK

Sequences:

>Translated_282_residues
MKRTTKGLAATLFLAIPLAACGDDSTDQADTTRWEEIQEEGTLTVGTAGTLYPASFREEESDTLTGFDVELMKEIAKRLD
LEIEFKEMAFDNMLTSVQNGQIDVAANDISVTEDRKEKFAFSKPYKYTYGTAIVRKSDLSGIESLEDLKGKKAAGEATTV
FMDVAREYGAEEVIYDNATNDQYLRDVSTGRTDVILNDYYLQTLALAFFPEFDITIHPDIAYNPQEVAFLMDKENDELQE
NIDRVLDEMLEDGTVKELSEQFYNGADVSVESDVDATIVETK
>Mature_282_residues
MKRTTKGLAATLFLAIPLAACGDDSTDQADTTRWEEIQEEGTLTVGTAGTLYPASFREEESDTLTGFDVELMKEIAKRLD
LEIEFKEMAFDNMLTSVQNGQIDVAANDISVTEDRKEKFAFSKPYKYTYGTAIVRKSDLSGIESLEDLKGKKAAGEATTV
FMDVAREYGAEEVIYDNATNDQYLRDVSTGRTDVILNDYYLQTLALAFFPEFDITIHPDIAYNPQEVAFLMDKENDELQE
NIDRVLDEMLEDGTVKELSEQFYNGADVSVESDVDATIVETK

Specific function: Probably part of a binding-protein-dependent transport system [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=258, Percent_Identity=33.3333333333333, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1787031, Length=226, Percent_Identity=26.9911504424779, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788648, Length=238, Percent_Identity=25.2100840336134, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787085, Length=225, Percent_Identity=24.4444444444444, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788649, Length=232, Percent_Identity=24.1379310344828, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787088, Length=216, Percent_Identity=23.1481481481481, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 31563; Mature: 31563

Theoretical pI: Translated: 3.92; Mature: 3.92

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRTTKGLAATLFLAIPLAACGDDSTDQADTTRWEEIQEEGTLTVGTAGTLYPASFREEE
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCCC
SDTLTGFDVELMKEIAKRLDLEIEFKEMAFDNMLTSVQNGQIDVAANDISVTEDRKEKFA
CCCCCCCCHHHHHHHHHHCCCEEEHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHC
FSKPYKYTYGTAIVRKSDLSGIESLEDLKGKKAAGEATTVFMDVAREYGAEEVIYDNATN
CCCCCCEECCEEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCC
DQYLRDVSTGRTDVILNDYYLQTLALAFFPEFDITIHPDIAYNPQEVAFLMDKENDELQE
HHHHHHHCCCCCEEEEHHHHHHHHHHHHCCCCCEEECCCCCCCHHHEEEEECCCCHHHHH
NIDRVLDEMLEDGTVKELSEQFYNGADVSVESDVDATIVETK
HHHHHHHHHHCCCCHHHHHHHHCCCCCCEECCCCCEEEEECC
>Mature Secondary Structure
MKRTTKGLAATLFLAIPLAACGDDSTDQADTTRWEEIQEEGTLTVGTAGTLYPASFREEE
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCCC
SDTLTGFDVELMKEIAKRLDLEIEFKEMAFDNMLTSVQNGQIDVAANDISVTEDRKEKFA
CCCCCCCCHHHHHHHHHHCCCEEEHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHC
FSKPYKYTYGTAIVRKSDLSGIESLEDLKGKKAAGEATTVFMDVAREYGAEEVIYDNATN
CCCCCCEECCEEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCC
DQYLRDVSTGRTDVILNDYYLQTLALAFFPEFDITIHPDIAYNPQEVAFLMDKENDELQE
HHHHHHHCCCCCEEEEHHHHHHHHHHHHCCCCCEEECCCCCCCHHHEEEEECCCCHHHHH
NIDRVLDEMLEDGTVKELSEQFYNGADVSVESDVDATIVETK
HHHHHHHHHHCCCCHHHHHHHHCCCCCCEECCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7704255; 9384377 [H]