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The map label for this gene is ywnE [H]

Identifier: 229915904

GI number: 229915904

Start: 148537

End: 149982

Strand: Reverse

Name: ywnE [H]

Synonym: EAT1b_0172

Alternate gene names: 229915904

Gene position: 149982-148537 (Counterclockwise)

Preceding gene: 229915908

Following gene: 229915901

Centisome position: 5.0

GC content: 48.34

Gene sequence:

>1446_bases
ATGATTTTCAGTTGGGGACTTTATATCGTCCTCGGGTTAAACATGTTGTTCGCACTCGCCGTCATCTTCGCCGAAAGACG
TGACGTCGGCGCGACCTGGGCCTGGCTTCTTGTCCTGTTTTTCTTACCAGTGCTCGGCTTTATCGTCTATCTCTTCCTAG
GGCGACAACTTAAAAAGGATAACTTCTATAATTTAAGTGTAGAAGAACGTTCCTTCCATTCGCTTCAAGTCGAAGCGCAA
CTTGAACAAATTCATACGCGCCAAACCCAGCTCGCGCAGCCGCTGTTTGAAAAGTATGACCAACTGCTTCAAATGAACTT
ACGATCTTCTAAATCGTTGATCAGTGTACATAACCGTTCCCTCATCTTGCACGACGGCGAACAAAAGTTTAAAGCGTTGA
TGGATGACATTCGCTCGGCCGAGACGGAGATCAATATTCAATATTACATCATCCAGCGTGACGCTCTCGGTCAGGCACTG
TTGAAAGAGTTGATCGAGCGTGCGAAAGCCGGCGTCAAAGTTCGACTCCTTTACGATGCGGTCGGCTCACGTAGTCTTCG
GAACTCGGACTTTAAAGAGTTGATTGCGAACGATGGCGAGGTGCGCGTCTTCTTCCCACCGACACTCGGGATCATCAACT
TCAAGTTGAACAACCGAAATCACCGAAAGGTCGTCATCATCGATGGGAAAATCGGCTATGTCGGTGGATTCAATGTCGGT
ACCGAGTATTTAGGTTTAGTCGAAAAATTTGGCTATTGGCGTGACACGCACCTGCGTGTCGAAGGAGATGTCGTTTACAA
TTTACAACACCGATTCATATTGGACTGGAATTATTCTGGTCACCGTAAACATGATCCTGAAAATGCCTTCTGCTTTGCGC
GACATGACATTAAAGAAAATTCTCCGATGCAAATCGTCACGAGTGGACCAAACTCGGATACGGAACATTTGAAGAATATG
ATGATCAAGATGATCATGTCCGCCAAACGACGGGTCGTCATTCAAACGCCATACTTCATTCCAGATACAAGCTTTATGGA
CGCTTGCAAAATGGCACTCCATTCTGGCGTTCAGATGGATATCATGATCCCTGGAAAACCGGACCATCCATTCGTCATGT
GGGCGTCCCTCTCGTTCCTTGGGGAACTCCTCGACTACGGGGCAAACGTCTATCTGTATGATGAAGGGTTTCTCCACGCG
AAGACGCTCGTCGTCGATGATGAGATCGCGACCGTCGGGACGACAAATTTGGACGCTCGAAGCTTCCGTCTCAATTTTGA
AGTGAACGCACTCATCTATGATGAGAGGGTCGCCCTCGAACTCGTTTCGTTATTCGAGACCGACACTGCGGTCAGTCGCA
CCTATACAGTGGAAGACTATGAATCCCGTAAATGGACGGTGCGCGCCCGTGAAGGGGTGTCACGCCTTCTTAGTCCGATC
CTGTGA

Upstream 100 bases:

>100_bases
CTTCTCCCCCTATTTCATTTGATAGCATCCTATTCCCTCATTTGATGTATGATTAAGCTATGAATCGATTATAAAGGGGT
GTATGTCGTTGGATTGGTCT

Downstream 100 bases:

>100_bases
ACTACTCACCACTTATTTGCTTCGCAAATGGAAGGGGGAGCTTCTTGGGAAGACCGTGCTCTCACTAGCCACGGTTATGG
ACCAAGCTCGTCGGGCAGTC

Product: phospholipase D/transphosphatidylase

Products: NA

Alternate protein names: CL synthase 2 [H]

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MIFSWGLYIVLGLNMLFALAVIFAERRDVGATWAWLLVLFFLPVLGFIVYLFLGRQLKKDNFYNLSVEERSFHSLQVEAQ
LEQIHTRQTQLAQPLFEKYDQLLQMNLRSSKSLISVHNRSLILHDGEQKFKALMDDIRSAETEINIQYYIIQRDALGQAL
LKELIERAKAGVKVRLLYDAVGSRSLRNSDFKELIANDGEVRVFFPPTLGIINFKLNNRNHRKVVIIDGKIGYVGGFNVG
TEYLGLVEKFGYWRDTHLRVEGDVVYNLQHRFILDWNYSGHRKHDPENAFCFARHDIKENSPMQIVTSGPNSDTEHLKNM
MIKMIMSAKRRVVIQTPYFIPDTSFMDACKMALHSGVQMDIMIPGKPDHPFVMWASLSFLGELLDYGANVYLYDEGFLHA
KTLVVDDEIATVGTTNLDARSFRLNFEVNALIYDERVALELVSLFETDTAVSRTYTVEDYESRKWTVRAREGVSRLLSPI
L

Sequences:

>Translated_481_residues
MIFSWGLYIVLGLNMLFALAVIFAERRDVGATWAWLLVLFFLPVLGFIVYLFLGRQLKKDNFYNLSVEERSFHSLQVEAQ
LEQIHTRQTQLAQPLFEKYDQLLQMNLRSSKSLISVHNRSLILHDGEQKFKALMDDIRSAETEINIQYYIIQRDALGQAL
LKELIERAKAGVKVRLLYDAVGSRSLRNSDFKELIANDGEVRVFFPPTLGIINFKLNNRNHRKVVIIDGKIGYVGGFNVG
TEYLGLVEKFGYWRDTHLRVEGDVVYNLQHRFILDWNYSGHRKHDPENAFCFARHDIKENSPMQIVTSGPNSDTEHLKNM
MIKMIMSAKRRVVIQTPYFIPDTSFMDACKMALHSGVQMDIMIPGKPDHPFVMWASLSFLGELLDYGANVYLYDEGFLHA
KTLVVDDEIATVGTTNLDARSFRLNFEVNALIYDERVALELVSLFETDTAVSRTYTVEDYESRKWTVRAREGVSRLLSPI
L
>Mature_481_residues
MIFSWGLYIVLGLNMLFALAVIFAERRDVGATWAWLLVLFFLPVLGFIVYLFLGRQLKKDNFYNLSVEERSFHSLQVEAQ
LEQIHTRQTQLAQPLFEKYDQLLQMNLRSSKSLISVHNRSLILHDGEQKFKALMDDIRSAETEINIQYYIIQRDALGQAL
LKELIERAKAGVKVRLLYDAVGSRSLRNSDFKELIANDGEVRVFFPPTLGIINFKLNNRNHRKVVIIDGKIGYVGGFNVG
TEYLGLVEKFGYWRDTHLRVEGDVVYNLQHRFILDWNYSGHRKHDPENAFCFARHDIKENSPMQIVTSGPNSDTEHLKNM
MIKMIMSAKRRVVIQTPYFIPDTSFMDACKMALHSGVQMDIMIPGKPDHPFVMWASLSFLGELLDYGANVYLYDEGFLHA
KTLVVDDEIATVGTTNLDARSFRLNFEVNALIYDERVALELVSLFETDTAVSRTYTVEDYESRKWTVRAREGVSRLLSPI
L

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=483, Percent_Identity=27.1221532091097, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1787007, Length=324, Percent_Identity=29.6296296296296, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI87081826, Length=371, Percent_Identity=25.8760107816712, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR015679
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 55401; Mature: 55401

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFSWGLYIVLGLNMLFALAVIFAERRDVGATWAWLLVLFFLPVLGFIVYLFLGRQLKKD
CEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NFYNLSVEERSFHSLQVEAQLEQIHTRQTQLAQPLFEKYDQLLQMNLRSSKSLISVHNRS
CCEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCE
LILHDGEQKFKALMDDIRSAETEINIQYYIIQRDALGQALLKELIERAKAGVKVRLLYDA
EEEECCHHHHHHHHHHHHCCCCEEEEEEEEEECHHHHHHHHHHHHHHHHCCEEEEEEEEC
VGSRSLRNSDFKELIANDGEVRVFFPPTLGIINFKLNNRNHRKVVIIDGKIGYVGGFNVG
CCCCCCCCCHHHHHHCCCCCEEEEECCCEEEEEEEECCCCCCEEEEEECCEEEEECCCCC
TEYLGLVEKFGYWRDTHLRVEGDVVYNLQHRFILDWNYSGHRKHDPENAFCFARHDIKEN
HHHHHHHHHHCCCCCCEEEEECCEEEEEEEEEEEEECCCCCCCCCCCCCEEEEECCCCCC
SPMQIVTSGPNSDTEHLKNMMIKMIMSAKRRVVIQTPYFIPDTSFMDACKMALHSGVQMD
CCEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEECCEECCCCHHHHHHHHHHHCCCEEE
IMIPGKPDHPFVMWASLSFLGELLDYGANVYLYDEGFLHAKTLVVDDEIATVGTTNLDAR
EEECCCCCCCEEEEHHHHHHHHHHHCCCCEEEEECCCEEEEEEEEECCEEEECCCCCCCE
SFRLNFEVNALIYDERVALELVSLFETDTAVSRTYTVEDYESRKWTVRAREGVSRLLSPI
EEEEEEEEEEEEECCHHHHHHHHHHHCCCHHCEEEEECCCCCCEEEEEHHHHHHHHHHHC
L
C
>Mature Secondary Structure
MIFSWGLYIVLGLNMLFALAVIFAERRDVGATWAWLLVLFFLPVLGFIVYLFLGRQLKKD
CEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NFYNLSVEERSFHSLQVEAQLEQIHTRQTQLAQPLFEKYDQLLQMNLRSSKSLISVHNRS
CCEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCE
LILHDGEQKFKALMDDIRSAETEINIQYYIIQRDALGQALLKELIERAKAGVKVRLLYDA
EEEECCHHHHHHHHHHHHCCCCEEEEEEEEEECHHHHHHHHHHHHHHHHCCEEEEEEEEC
VGSRSLRNSDFKELIANDGEVRVFFPPTLGIINFKLNNRNHRKVVIIDGKIGYVGGFNVG
CCCCCCCCCHHHHHHCCCCCEEEEECCCEEEEEEEECCCCCCEEEEEECCEEEEECCCCC
TEYLGLVEKFGYWRDTHLRVEGDVVYNLQHRFILDWNYSGHRKHDPENAFCFARHDIKEN
HHHHHHHHHHCCCCCCEEEEECCEEEEEEEEEEEEECCCCCCCCCCCCCEEEEECCCCCC
SPMQIVTSGPNSDTEHLKNMMIKMIMSAKRRVVIQTPYFIPDTSFMDACKMALHSGVQMD
CCEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEECCEECCCCHHHHHHHHHHHCCCEEE
IMIPGKPDHPFVMWASLSFLGELLDYGANVYLYDEGFLHAKTLVVDDEIATVGTTNLDAR
EEECCCCCCCEEEEHHHHHHHHHHHCCCCEEEEECCCEEEEEEEEECCEEEECCCCCCCE
SFRLNFEVNALIYDERVALELVSLFETDTAVSRTYTVEDYESRKWTVRAREGVSRLLSPI
EEEEEEEEEEEEECCHHHHHHHHHHHCCCHHCEEEEECCCCCCEEEEEHHHHHHHHHHHC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9150240; 9384377 [H]