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The map label for this gene is yhjG [H]

Identifier: 229915799

GI number: 229915799

Start: 47816

End: 49354

Strand: Reverse

Name: yhjG [H]

Synonym: EAT1b_0066

Alternate gene names: 229915799

Gene position: 49354-47816 (Counterclockwise)

Preceding gene: 229915813

Following gene: 229915797

Centisome position: 1.65

GC content: 49.06

Gene sequence:

>1539_bases
GTGGACGAACAAGTAGATGTATTGATTGTCGGTGCCGGACCAACCGGCCTGACGCTCGGACTCACCTTAGCCAGATATGG
CGTATCTTTTCAAATCATTGATCGTAAGACTACCCCGTCGGACAATTCACGAGCTATCGGGATTCAGCCGCGGACCATCG
AAGTGTTTTCTCGTTTAGATGTTGCGCAAGAAGTGCTAGAGCGTGCCCGAACAATCGAGCGGGGGAATCTATATTTTTCG
GGACAGTGGACAGCAAAGCTTGAGTTCTCCAAACTCGTGACCCCTTATCCGTTCGTGACGTTGTTACGTCAAAATGAAAC
CGAAGAGATTTTGGAACGCGCCCTACGAAAACATGGACATGTTGTGAAGCGTGGGGAAACCTTGTCATCCATTACCCAAT
ATCCATCCCGTACCATCGCACATTTGACCGCGACTGATGGGATGACTCGTGCCGTTGAAGCGAAATATGTTGTCGCCGCC
GATGGAGCAAACAGCTCGGTTCGTCGGATGCTCGCCCTTCCGTTTAGCGGTAAATCATTTAAAGAGGCTTGGGTGTTAGC
CGATTTAAAGGCGGATTGGCCGATATCCCGGGAAGAGGTTCATATCTTTTTTTCGGATCGAGGAGTGCTTGAAGTCTTCC
CGCTCACGGATGAAACAATTCGTATCACGGGAAACTTGAGAACGGATGAATCATTCCAAGAATCGGATCTTCAAGAATTG
GTGGAGCAACGCAGTCATATGCCGGTCCATATTCACGATGTTGAATGGTTCTCTTTATTCCGTGTGCACAATCGCATGGT
CGATACGTTCATCCATAATCGCATCATTCTCATGGGGGATGCGGCACATATTAACTCCCCGGTCGGCGGACAAGGGATGA
ACACCGGGATAGCGGATGCCTTCAACTTAGGTTGGAAACTGTGGTTACATCTCAAAACTGGCGCTCCCCATCACGTCGTG
TCCACCTATCGGGACGAGCGCGAACATGTTGCCCGCAATGTGCTACAGACGACGAATATCGCAACGGAGATGTTGCAGAC
GACCCTCCCGATGCTCTTGCCGTTCCAAGCAATCGGTGCGGATGTCTTCACTCGCGTCAATCTCGTCAACCAAAAGATCA
CCGAACGGATCTCTCAGCTCCATTTATCGTATCCGAATGCGCTTCGGTTACCGTTTCGTCGCGTCGCGGAAGGAAAGATC
TTTCCGGAGACTGAGATTTTACGAACGGATGACGGGTTTCGGACAAAAATATCCGAAATCGCGGACGGACGGTTTCAAGT
GCTCGTATTTGACGACGGGAAACGTGATTTGACGTCCATTTATGACTATTTGAACAAGAACCAGCCGTTCATTCAAACGA
TTACCTTATCTAAGACGAGTCGCCCGTTTTTCGATCAAGGAAACGAACTAGCCAAATCACTTTTTATGAAACGTGGCATA
TTAGTCATTCGTCCAGACGGTTATTTGTTGCATAAGCAACATCATGTTCGCTTAAAGCCGTTACAGGACAAGTTGGCCGT
CTGGCTGCCTTCCTCTTAA

Upstream 100 bases:

>100_bases
CCTTCCATCATCGAATATCATATATCGTTCTATACCCTACTTTTCGTTTAGTCGTTGCCAATGTCGGAAATATCATTATT
AATGATAGGAGTTGATCAGC

Downstream 100 bases:

>100_bases
ACGTGTATAAATGGAGAGCCTCACCGAATGGGTGAAGCTCTCCATGTCATGACTATTCAACGTTTACATATAGAATACCT
GGTTTCGAGAGCGTCAAATC

Product: monooxygenase FAD-binding

Products: H2O; 3-(2,3-dihydroxyphenyl)propionate; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MDEQVDVLIVGAGPTGLTLGLTLARYGVSFQIIDRKTTPSDNSRAIGIQPRTIEVFSRLDVAQEVLERARTIERGNLYFS
GQWTAKLEFSKLVTPYPFVTLLRQNETEEILERALRKHGHVVKRGETLSSITQYPSRTIAHLTATDGMTRAVEAKYVVAA
DGANSSVRRMLALPFSGKSFKEAWVLADLKADWPISREEVHIFFSDRGVLEVFPLTDETIRITGNLRTDESFQESDLQEL
VEQRSHMPVHIHDVEWFSLFRVHNRMVDTFIHNRIILMGDAAHINSPVGGQGMNTGIADAFNLGWKLWLHLKTGAPHHVV
STYRDEREHVARNVLQTTNIATEMLQTTLPMLLPFQAIGADVFTRVNLVNQKITERISQLHLSYPNALRLPFRRVAEGKI
FPETEILRTDDGFRTKISEIADGRFQVLVFDDGKRDLTSIYDYLNKNQPFIQTITLSKTSRPFFDQGNELAKSLFMKRGI
LVIRPDGYLLHKQHHVRLKPLQDKLAVWLPSS

Sequences:

>Translated_512_residues
MDEQVDVLIVGAGPTGLTLGLTLARYGVSFQIIDRKTTPSDNSRAIGIQPRTIEVFSRLDVAQEVLERARTIERGNLYFS
GQWTAKLEFSKLVTPYPFVTLLRQNETEEILERALRKHGHVVKRGETLSSITQYPSRTIAHLTATDGMTRAVEAKYVVAA
DGANSSVRRMLALPFSGKSFKEAWVLADLKADWPISREEVHIFFSDRGVLEVFPLTDETIRITGNLRTDESFQESDLQEL
VEQRSHMPVHIHDVEWFSLFRVHNRMVDTFIHNRIILMGDAAHINSPVGGQGMNTGIADAFNLGWKLWLHLKTGAPHHVV
STYRDEREHVARNVLQTTNIATEMLQTTLPMLLPFQAIGADVFTRVNLVNQKITERISQLHLSYPNALRLPFRRVAEGKI
FPETEILRTDDGFRTKISEIADGRFQVLVFDDGKRDLTSIYDYLNKNQPFIQTITLSKTSRPFFDQGNELAKSLFMKRGI
LVIRPDGYLLHKQHHVRLKPLQDKLAVWLPSS
>Mature_512_residues
MDEQVDVLIVGAGPTGLTLGLTLARYGVSFQIIDRKTTPSDNSRAIGIQPRTIEVFSRLDVAQEVLERARTIERGNLYFS
GQWTAKLEFSKLVTPYPFVTLLRQNETEEILERALRKHGHVVKRGETLSSITQYPSRTIAHLTATDGMTRAVEAKYVVAA
DGANSSVRRMLALPFSGKSFKEAWVLADLKADWPISREEVHIFFSDRGVLEVFPLTDETIRITGNLRTDESFQESDLQEL
VEQRSHMPVHIHDVEWFSLFRVHNRMVDTFIHNRIILMGDAAHINSPVGGQGMNTGIADAFNLGWKLWLHLKTGAPHHVV
STYRDEREHVARNVLQTTNIATEMLQTTLPMLLPFQAIGADVFTRVNLVNQKITERISQLHLSYPNALRLPFRRVAEGKI
FPETEILRTDDGFRTKISEIADGRFQVLVFDDGKRDLTSIYDYLNKNQPFIQTITLSKTSRPFFDQGNELAKSLFMKRGI
LVIRPDGYLLHKQHHVRLKPLQDKLAVWLPSS

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=364, Percent_Identity=26.6483516483516, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1789273, Length=322, Percent_Identity=23.9130434782609, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 58245; Mature: 58245

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEQVDVLIVGAGPTGLTLGLTLARYGVSFQIIDRKTTPSDNSRAIGIQPRTIEVFSRLD
CCCCEEEEEEECCCCCHHHHHHHHHHCCEEEEEECCCCCCCCCEEEECCCCHHHHHHHHH
VAQEVLERARTIERGNLYFSGQWTAKLEFSKLVTPYPFVTLLRQNETEEILERALRKHGH
HHHHHHHHHHHHHCCCEEECCEEEEEEEHHHCCCCCCHHHEEECCCHHHHHHHHHHHCCC
VVKRGETLSSITQYPSRTIAHLTATDGMTRAVEAKYVVAADGANSSVRRMLALPFSGKSF
EEECCCHHHHHHHCCCCCEEEEEECCCCCCEEEEEEEEEECCCCHHHHHEEECCCCCCCC
KEAWVLADLKADWPISREEVHIFFSDRGVLEVFPLTDETIRITGNLRTDESFQESDLQEL
CCEEEEEECCCCCCCCCCEEEEEEECCCEEEEEECCCCEEEEEECCCCCCCHHHHHHHHH
VEQRSHMPVHIHDVEWFSLFRVHNRMVDTFIHNRIILMGDAAHINSPVGGQGMNTGIADA
HHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHCEEEEEECHHHCCCCCCCCCCCCCCHHH
FNLGWKLWLHLKTGAPHHVVSTYRDEREHVARNVLQTTNIATEMLQTTLPMLLPFQAIGA
HCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
DVFTRVNLVNQKITERISQLHLSYPNALRLPFRRVAEGKIFPETEILRTDDGFRTKISEI
HHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCHHHHHCCCCCCCCEEEECCCCHHHHHHHH
ADGRFQVLVFDDGKRDLTSIYDYLNKNQPFIQTITLSKTSRPFFDQGNELAKSLFMKRGI
CCCEEEEEEEECCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCE
LVIRPDGYLLHKQHHVRLKPLQDKLAVWLPSS
EEECCCCEEEECCCCEEECCCCCCEEEECCCC
>Mature Secondary Structure
MDEQVDVLIVGAGPTGLTLGLTLARYGVSFQIIDRKTTPSDNSRAIGIQPRTIEVFSRLD
CCCCEEEEEEECCCCCHHHHHHHHHHCCEEEEEECCCCCCCCCEEEECCCCHHHHHHHHH
VAQEVLERARTIERGNLYFSGQWTAKLEFSKLVTPYPFVTLLRQNETEEILERALRKHGH
HHHHHHHHHHHHHCCCEEECCEEEEEEEHHHCCCCCCHHHEEECCCHHHHHHHHHHHCCC
VVKRGETLSSITQYPSRTIAHLTATDGMTRAVEAKYVVAADGANSSVRRMLALPFSGKSF
EEECCCHHHHHHHCCCCCEEEEEECCCCCCEEEEEEEEEECCCCHHHHHEEECCCCCCCC
KEAWVLADLKADWPISREEVHIFFSDRGVLEVFPLTDETIRITGNLRTDESFQESDLQEL
CCEEEEEECCCCCCCCCCEEEEEEECCCEEEEEECCCCEEEEEECCCCCCCHHHHHHHHH
VEQRSHMPVHIHDVEWFSLFRVHNRMVDTFIHNRIILMGDAAHINSPVGGQGMNTGIADA
HHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHCEEEEEECHHHCCCCCCCCCCCCCCHHH
FNLGWKLWLHLKTGAPHHVVSTYRDEREHVARNVLQTTNIATEMLQTTLPMLLPFQAIGA
HCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
DVFTRVNLVNQKITERISQLHLSYPNALRLPFRRVAEGKIFPETEILRTDDGFRTKISEI
HHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCHHHHHCCCCCCCCEEEECCCCHHHHHHHH
ADGRFQVLVFDDGKRDLTSIYDYLNKNQPFIQTITLSKTSRPFFDQGNELAKSLFMKRGI
CCCEEEEEEEECCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCE
LVIRPDGYLLHKQHHVRLKPLQDKLAVWLPSS
EEECCCCEEEECCCCEEECCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-(3-hydroxyphenyl)propionate; NADH; O2 [C]

Specific reaction: 3-(3-hydroxyphenyl)propionate + NADH + O2 = H2O + 3-(2,3-dihydroxyphenyl)propionate + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]