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The map label for this gene is hemK

Identifier: 229587214

GI number: 229587214

Start: 1226885

End: 1228459

Strand: Direct

Name: hemK

Synonym: RAF_ORF1201

Alternate gene names: 229587214

Gene position: 1226885-1228459 (Clockwise)

Preceding gene: 229587213

Following gene: 229587224

Centisome position: 95.96

GC content: 31.68

Gene sequence:

>1575_bases
ATGCAATGTTCTATAAAGCAAATTCTTAGTGACGCTACAGATAAATTAAATAAAATAGGTATTAGTTCGCCGCAATTAGA
AGCACGAATTTTACTACAGCATGTTATAAATAAACCTATTGAGTATTTACTTATTAATCTTGATGAACAGTTAAATGAAG
CCAAGATAGAAGCTTTTGAAAAACTGTTAGCGAGAAGATTAAAGCATGAACCGATAGTATATATTACAGGTGTTAAAGAA
TTTTACTCACGTGAATTTATTGTTAATAAACATGTATTAATTCCAAGAAGCGATACTGAAGTTTTGGTGGATGTTGTATT
TCAATGTCATTCCCGCGAAAGCGGGAATCCAGAAAAAAAGCAACCGAATCCCTGCTTTCGCGGGAATGACATAAGCGAAA
ACTGCAATGACAAATTCTTAAATATCCTAGAACTTGGCACAGGTAGCGGCTGCATTGCTATCAGTCTATTATGTGAGTTA
CCGAATGCTAATGTAATTGCAACCGATATAAGTCTTGATGCTATAGACATTATCAAAAGTAATGCGGCAAAATATGAGGT
AACGGATCGCATTCAGATTATTCACAGTAATTGGTTTGAGAATATTGAAACTCAGAAATTTGATTTTATCGTTAGCAACC
CACCATATATAGCTCACAGCGAAAAATCAGAGATGGCAATTGAAACAATCAATTATGAACCGTCTATTGCTTTATTTGCT
GAAAAAGACGGACTGCAAGCTTACTTTCTCATCGCTGAAAATGCCAAACAATTCTTAAAACCAAATGGTAAGATTATATT
AGAAATAGGGTTTAAACAGGAAGAAGCGGTAACCCAAATTTTCTTAGATCATGGTTATAATATTGAAAGCGTTTATAAGG
ATCTACAAGGTCACAGTAGGGTAATATTATTTTCTCCTATTAATCTCAATCGGTCATATGCAAGACGTATCGGTAAAAGC
TTATCAGGAGTGCAAAAGAATTTATTAGATAACAAACTACCAAAATATTTATTTTCTAAAGAAAAACTTGTAAATGAAAA
ACGTAAAGTATTTCTTGAGATAGGCTTTGGTATGGGTGAGCATTTCATTAATCAAGCTAAAATGAATCCTAATGCACTTT
TTATTGGGATAGAGGTATATTTAAACGGAGTTGCAAATGTTTTAAAGCTTGCAGGCGAACAAAATATCACGAATTTTTTA
TTATTCCCTAATAATTTGGATTTAATATTAAATGAGATACCAAGTAATAGCCTTGATGGAATTTATATTTTATTTCCCGA
TCCATGGATAAAAAATAAGCAAAAAAAGAAACGTATTTTCAATAAAGAACGACTTAAGCTTTTACAAGATAAGCTAAAAG
ACAATGGTAATTTAGTATTTGCTTCCGATATCGAAAATTATTTTTATGAAGCAATAGAGTTAATACAGCAGAACGGTAAT
TTTGAAATTATAAATAAAAATGATTATTTAAAACCGCACGATAACTATGTAATTACCAAATATCATCAAAAAGCTATTAA
GGCGAATAGAACGCCGAAATTTATGATTTTGCGGCACGTTTTAGGCGATCATTAA

Upstream 100 bases:

>100_bases
ATATATTTCGTGAATTTACTGAACTTAAAAAACGAGCACCGCTAGGTAGTGTAGATGAGATTTTAGCAATGAAAAATTTT
GGCAGAAAATAAAACTTTCC

Downstream 100 bases:

>100_bases
TTGCAACCCCTATATTCACTTGAGGTATAGGGCTAACTGCTATACCTCTTACATCATGAGATGCAGCATAATCGTCAAGT
ATTCTTAAATAAGCATAAAG

Product: bifunctional N5-glutamine S-adenosyl-L-methionine-dependent methyltransferase/tRNA (m7G46) methyltransferase

Products: NA

Alternate protein names: HemK protein homolog; M.RcoHemKP; tRNA (guanine-N(7)-)-methyltransferase; tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 524; Mature: 524

Protein sequence:

>524_residues
MQCSIKQILSDATDKLNKIGISSPQLEARILLQHVINKPIEYLLINLDEQLNEAKIEAFEKLLARRLKHEPIVYITGVKE
FYSREFIVNKHVLIPRSDTEVLVDVVFQCHSRESGNPEKKQPNPCFRGNDISENCNDKFLNILELGTGSGCIAISLLCEL
PNANVIATDISLDAIDIIKSNAAKYEVTDRIQIIHSNWFENIETQKFDFIVSNPPYIAHSEKSEMAIETINYEPSIALFA
EKDGLQAYFLIAENAKQFLKPNGKIILEIGFKQEEAVTQIFLDHGYNIESVYKDLQGHSRVILFSPINLNRSYARRIGKS
LSGVQKNLLDNKLPKYLFSKEKLVNEKRKVFLEIGFGMGEHFINQAKMNPNALFIGIEVYLNGVANVLKLAGEQNITNFL
LFPNNLDLILNEIPSNSLDGIYILFPDPWIKNKQKKKRIFNKERLKLLQDKLKDNGNLVFASDIENYFYEAIELIQQNGN
FEIINKNDYLKPHDNYVITKYHQKAIKANRTPKFMILRHVLGDH

Sequences:

>Translated_524_residues
MQCSIKQILSDATDKLNKIGISSPQLEARILLQHVINKPIEYLLINLDEQLNEAKIEAFEKLLARRLKHEPIVYITGVKE
FYSREFIVNKHVLIPRSDTEVLVDVVFQCHSRESGNPEKKQPNPCFRGNDISENCNDKFLNILELGTGSGCIAISLLCEL
PNANVIATDISLDAIDIIKSNAAKYEVTDRIQIIHSNWFENIETQKFDFIVSNPPYIAHSEKSEMAIETINYEPSIALFA
EKDGLQAYFLIAENAKQFLKPNGKIILEIGFKQEEAVTQIFLDHGYNIESVYKDLQGHSRVILFSPINLNRSYARRIGKS
LSGVQKNLLDNKLPKYLFSKEKLVNEKRKVFLEIGFGMGEHFINQAKMNPNALFIGIEVYLNGVANVLKLAGEQNITNFL
LFPNNLDLILNEIPSNSLDGIYILFPDPWIKNKQKKKRIFNKERLKLLQDKLKDNGNLVFASDIENYFYEAIELIQQNGN
FEIINKNDYLKPHDNYVITKYHQKAIKANRTPKFMILRHVLGDH
>Mature_524_residues
MQCSIKQILSDATDKLNKIGISSPQLEARILLQHVINKPIEYLLINLDEQLNEAKIEAFEKLLARRLKHEPIVYITGVKE
FYSREFIVNKHVLIPRSDTEVLVDVVFQCHSRESGNPEKKQPNPCFRGNDISENCNDKFLNILELGTGSGCIAISLLCEL
PNANVIATDISLDAIDIIKSNAAKYEVTDRIQIIHSNWFENIETQKFDFIVSNPPYIAHSEKSEMAIETINYEPSIALFA
EKDGLQAYFLIAENAKQFLKPNGKIILEIGFKQEEAVTQIFLDHGYNIESVYKDLQGHSRVILFSPINLNRSYARRIGKS
LSGVQKNLLDNKLPKYLFSKEKLVNEKRKVFLEIGFGMGEHFINQAKMNPNALFIGIEVYLNGVANVLKLAGEQNITNFL
LFPNNLDLILNEIPSNSLDGIYILFPDPWIKNKQKKKRIFNKERLKLLQDKLKDNGNLVFASDIENYFYEAIELIQQNGN
FEIINKNDYLKPHDNYVITKYHQKAIKANRTPKFMILRHVLGDH

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Homo sapiens, GI7705409, Length=310, Percent_Identity=30.6451612903226, Blast_Score=113, Evalue=4e-25,
Organism=Escherichia coli, GI1787463, Length=281, Percent_Identity=34.5195729537367, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI87082085, Length=257, Percent_Identity=30.3501945525292, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1789330, Length=209, Percent_Identity=32.5358851674641, Blast_Score=104, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24582226, Length=265, Percent_Identity=26.7924528301887, Blast_Score=102, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEMK_RICCN (Q92G13)

Other databases:

- EMBL:   AE006914
- PIR:   B97864
- RefSeq:   NP_360951.1
- ProteinModelPortal:   Q92G13
- GeneID:   928460
- GenomeReviews:   AE006914_GR
- KEGG:   rco:RC1314
- NMPDR:   fig|272944.1.peg.1314
- OMA:   IGVEVYL
- ProtClustDB:   PRK01544
- BioCyc:   RCON272944:RC1314-MONOMER
- BRENDA:   2.1.1.33
- HAMAP:   MF_01057
- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848
- InterPro:   IPR003358
- TIGRFAMs:   TIGR00091
- TIGRFAMs:   TIGR00536
- TIGRFAMs:   TIGR03534

Pfam domain/function: PF02390 Methyltransf_4; PF05175 MTS

EC number: =2.1.1.33

Molecular weight: Translated: 60080; Mature: 60080

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: PS00092 N6_MTASE

Important sites: BINDING 169-169 BINDING 198-198 BINDING 213-213 BINDING 353-353 BINDING 378-378 BINDING 405-405 BINDING 427-427 BINDING 431-431 BINDING 463-463

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQCSIKQILSDATDKLNKIGISSPQLEARILLQHVINKPIEYLLINLDEQLNEAKIEAFE
CCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHH
KLLARRLKHEPIVYITGVKEFYSREFIVNKHVLIPRSDTEVLVDVVFQCHSRESGNPEKK
HHHHHHHCCCCEEEEECCHHHHCCCEEEECEEEECCCCHHHHHHHHHHHHCCCCCCCCCC
QPNPCFRGNDISENCNDKFLNILELGTGSGCIAISLLCELPNANVIATDISLDAIDIIKS
CCCCCCCCCCCCCCCCHHHHHHEEECCCCCCEEEEEEHCCCCCCEEEEECCCCHHHHHHC
NAAKYEVTDRIQIIHSNWFENIETQKFDFIVSNPPYIAHSEKSEMAIETINYEPSIALFA
CCCCEEEHHHHEEEHHHHHCCCCCCEEEEEECCCCEEEECCCCCEEEEEECCCCCEEEEE
EKDGLQAYFLIAENAKQFLKPNGKIILEIGFKQEEAVTQIFLDHGYNIESVYKDLQGHSR
ECCCCEEEEEEECCHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHCCCCE
VILFSPINLNRSYARRIGKSLSGVQKNLLDNKLPKYLFSKEKLVNEKRKVFLEIGFGMGE
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCH
HFINQAKMNPNALFIGIEVYLNGVANVLKLAGEQNITNFLLFPNNLDLILNEIPSNSLDG
HHHHHCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHEECCCCCCCCE
IYILFPDPWIKNKQKKKRIFNKERLKLLQDKLKDNGNLVFASDIENYFYEAIELIQQNGN
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCCCC
FEIINKNDYLKPHDNYVITKYHQKAIKANRTPKFMILRHVLGDH
EEEECCCCCCCCCCCEEEEHHHHHHHHCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MQCSIKQILSDATDKLNKIGISSPQLEARILLQHVINKPIEYLLINLDEQLNEAKIEAFE
CCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHH
KLLARRLKHEPIVYITGVKEFYSREFIVNKHVLIPRSDTEVLVDVVFQCHSRESGNPEKK
HHHHHHHCCCCEEEEECCHHHHCCCEEEECEEEECCCCHHHHHHHHHHHHCCCCCCCCCC
QPNPCFRGNDISENCNDKFLNILELGTGSGCIAISLLCELPNANVIATDISLDAIDIIKS
CCCCCCCCCCCCCCCCHHHHHHEEECCCCCCEEEEEEHCCCCCCEEEEECCCCHHHHHHC
NAAKYEVTDRIQIIHSNWFENIETQKFDFIVSNPPYIAHSEKSEMAIETINYEPSIALFA
CCCCEEEHHHHEEEHHHHHCCCCCCEEEEEECCCCEEEECCCCCEEEEEECCCCCEEEEE
EKDGLQAYFLIAENAKQFLKPNGKIILEIGFKQEEAVTQIFLDHGYNIESVYKDLQGHSR
ECCCCEEEEEEECCHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHCCCCE
VILFSPINLNRSYARRIGKSLSGVQKNLLDNKLPKYLFSKEKLVNEKRKVFLEIGFGMGE
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCH
HFINQAKMNPNALFIGIEVYLNGVANVLKLAGEQNITNFLLFPNNLDLILNEIPSNSLDG
HHHHHCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHEECCCCCCCCE
IYILFPDPWIKNKQKKKRIFNKERLKLLQDKLKDNGNLVFASDIENYFYEAIELIQQNGN
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCCCC
FEIINKNDYLKPHDNYVITKYHQKAIKANRTPKFMILRHVLGDH
EEEECCCCCCCCCCCEEEEHHHHHHHHCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893